Storage of Fresh Bovine Semen in a Diluent Based on the Ionic Composition of Cauda Epididymal Plasma
Contents For artificial insemination (AI) in cattle, much lower insemination doses can be applied when fresh semen is used instead of frozen–thawed semen. However, a particular disadvantage of fresh semen is its limited shelf life. As bovine spermatozoa can be stored for several weeks in the cauda e...
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| Veröffentlicht in: | Reproduction in domestic animals 2004-12, Vol.39 (6), p.410-416 |
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| container_title | Reproduction in domestic animals |
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| creator | Verberckmoes, S Van Soom, A Dewulf, J De Pauw, I De Kruif, A |
| description | Contents
For artificial insemination (AI) in cattle, much lower insemination doses can be applied when fresh semen is used instead of frozen–thawed semen. However, a particular disadvantage of fresh semen is its limited shelf life. As bovine spermatozoa can be stored for several weeks in the cauda epididymis without negative effects on their fertilizing capacity, it is an interesting organ to serve as a model in order to prolong the shelf life of fresh semen. First, the storage capacity of a diluent [cauda epididymal plasma (CEP‐1)] with the same ionic composition, pH and osmolarity as the bovine CEP was compared with a Tris diluent for extended preservation of fresh ejaculated bovine semen. Secondly, the ionic composition of the CEP‐1 diluent was modified (CEP‐2) and its storage capacity was compared with this of the CEP‐1 and Tris diluent. Finally, the effect of addition of different polyols (sorbitol, glycerol, mannitol) and egg yolk concentrations (5, 10 and 20%) to the CEP‐2 diluent was assessed. Sperm quality decreased rapidly in the CEP‐1 diluent. The quality and especially progressive motility of spermatozoa stored in the CEP‐2 diluent were better those in the CEP‐1 and Tris diluent. No significant effects of different sugars or egg yolk concentrations on the quality of fresh bovine semen in the CEP‐2 diluent were observed. In conclusion, the CEP‐2 diluent with 10% egg yolk and 1 g/l sorbitol may be used for extended preservation of fresh bovine semen at 5°C up to 6 days. |
| doi_str_mv | 10.1111/j.1439-0531.2004.00521.x |
| format | Article |
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For artificial insemination (AI) in cattle, much lower insemination doses can be applied when fresh semen is used instead of frozen–thawed semen. However, a particular disadvantage of fresh semen is its limited shelf life. As bovine spermatozoa can be stored for several weeks in the cauda epididymis without negative effects on their fertilizing capacity, it is an interesting organ to serve as a model in order to prolong the shelf life of fresh semen. First, the storage capacity of a diluent [cauda epididymal plasma (CEP‐1)] with the same ionic composition, pH and osmolarity as the bovine CEP was compared with a Tris diluent for extended preservation of fresh ejaculated bovine semen. Secondly, the ionic composition of the CEP‐1 diluent was modified (CEP‐2) and its storage capacity was compared with this of the CEP‐1 and Tris diluent. Finally, the effect of addition of different polyols (sorbitol, glycerol, mannitol) and egg yolk concentrations (5, 10 and 20%) to the CEP‐2 diluent was assessed. Sperm quality decreased rapidly in the CEP‐1 diluent. The quality and especially progressive motility of spermatozoa stored in the CEP‐2 diluent were better those in the CEP‐1 and Tris diluent. No significant effects of different sugars or egg yolk concentrations on the quality of fresh bovine semen in the CEP‐2 diluent were observed. In conclusion, the CEP‐2 diluent with 10% egg yolk and 1 g/l sorbitol may be used for extended preservation of fresh bovine semen at 5°C up to 6 days.</description><identifier>ISSN: 0936-6768</identifier><identifier>EISSN: 1439-0531</identifier><identifier>DOI: 10.1111/j.1439-0531.2004.00521.x</identifier><identifier>PMID: 15598230</identifier><language>eng</language><publisher>Berlin, Germany: Blackwell Verlag GmbH</publisher><subject>Animal reproduction ; Animals ; Artificial insemination ; Biological and medical sciences ; Cattle ; Egg Yolk ; Embryology: invertebrates and vertebrates. Teratology ; Epididymis ; Fundamental and applied biological sciences. Psychology ; Hydrogen-Ion Concentration ; Ions ; Male ; Osmolar Concentration ; Plasma ; Polymers ; Semen ; Semen Preservation - methods ; Semen Preservation - veterinary ; Solutions ; Sorbitol ; Sperm Motility ; Spermatozoa - physiology ; Time Factors</subject><ispartof>Reproduction in domestic animals, 2004-12, Vol.39 (6), p.410-416</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright Blackwell Publishing Dec 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4611-e743e658130d1e46d880d0d7f0122302b7357bf57e719399979b37a04e7bd8b13</citedby><cites>FETCH-LOGICAL-c4611-e743e658130d1e46d880d0d7f0122302b7357bf57e719399979b37a04e7bd8b13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1439-0531.2004.00521.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1439-0531.2004.00521.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16343729$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15598230$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Verberckmoes, S</creatorcontrib><creatorcontrib>Van Soom, A</creatorcontrib><creatorcontrib>Dewulf, J</creatorcontrib><creatorcontrib>De Pauw, I</creatorcontrib><creatorcontrib>De Kruif, A</creatorcontrib><title>Storage of Fresh Bovine Semen in a Diluent Based on the Ionic Composition of Cauda Epididymal Plasma</title><title>Reproduction in domestic animals</title><addtitle>Reprod Domest Anim</addtitle><description>Contents
For artificial insemination (AI) in cattle, much lower insemination doses can be applied when fresh semen is used instead of frozen–thawed semen. However, a particular disadvantage of fresh semen is its limited shelf life. As bovine spermatozoa can be stored for several weeks in the cauda epididymis without negative effects on their fertilizing capacity, it is an interesting organ to serve as a model in order to prolong the shelf life of fresh semen. First, the storage capacity of a diluent [cauda epididymal plasma (CEP‐1)] with the same ionic composition, pH and osmolarity as the bovine CEP was compared with a Tris diluent for extended preservation of fresh ejaculated bovine semen. Secondly, the ionic composition of the CEP‐1 diluent was modified (CEP‐2) and its storage capacity was compared with this of the CEP‐1 and Tris diluent. Finally, the effect of addition of different polyols (sorbitol, glycerol, mannitol) and egg yolk concentrations (5, 10 and 20%) to the CEP‐2 diluent was assessed. Sperm quality decreased rapidly in the CEP‐1 diluent. The quality and especially progressive motility of spermatozoa stored in the CEP‐2 diluent were better those in the CEP‐1 and Tris diluent. No significant effects of different sugars or egg yolk concentrations on the quality of fresh bovine semen in the CEP‐2 diluent were observed. In conclusion, the CEP‐2 diluent with 10% egg yolk and 1 g/l sorbitol may be used for extended preservation of fresh bovine semen at 5°C up to 6 days.</description><subject>Animal reproduction</subject><subject>Animals</subject><subject>Artificial insemination</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Egg Yolk</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Epididymis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Ions</subject><subject>Male</subject><subject>Osmolar Concentration</subject><subject>Plasma</subject><subject>Polymers</subject><subject>Semen</subject><subject>Semen Preservation - methods</subject><subject>Semen Preservation - veterinary</subject><subject>Solutions</subject><subject>Sorbitol</subject><subject>Sperm Motility</subject><subject>Spermatozoa - physiology</subject><subject>Time Factors</subject><issn>0936-6768</issn><issn>1439-0531</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkV1v0zAYhS0EYmXwF5CFxO5S7Di2Y4mbNd2XNAZiICRuLCd-w1ySuNgJtP9-Lq02iSt8Y8t-ztHxeRHClMxpWu9Wc1owlRHO6DwnpJgTwnM63zxBs4eHp2hGFBOZkKI8Qi9iXBFCeSnlc3REOVdlzsgM2dvRB_MDsG_xeYB4hxf-txsA30IPA3YDNnjpugmGES9MBIv9gMc7wFd-cA2ufL_20Y0u3SaHykzW4LO1s85ue9PhT52JvXmJnrWmi_DqsB-jr-dnX6rL7PrjxVV1ep01haA0A1kwELykjFgKhbBlSSyxsiU0T2HzWjIu65ZLkFQxpZRUNZOGFCBrW9aUHaOTve86-F8TxFH3LjbQdWYAP0UtJE2_VjyBb_4BV34KQ8qmc8pkUZZSJKjcQ03wMQZo9Tq43oStpkTvxqBXete23rWtd2PQf8egN0n6-uA_1T3YR-Gh9wS8PQAmNqZrgxkaFx85wQomc5W493vuj-tg-98B9OflaTokebaXuzjC5kFuws_UBZNcf7u50N_FcrGs8hv9gd0Dsr2uyA</recordid><startdate>200412</startdate><enddate>200412</enddate><creator>Verberckmoes, S</creator><creator>Van Soom, A</creator><creator>Dewulf, J</creator><creator>De Pauw, I</creator><creator>De Kruif, A</creator><general>Blackwell Verlag GmbH</general><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200412</creationdate><title>Storage of Fresh Bovine Semen in a Diluent Based on the Ionic Composition of Cauda Epididymal Plasma</title><author>Verberckmoes, S ; Van Soom, A ; Dewulf, J ; De Pauw, I ; De Kruif, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4611-e743e658130d1e46d880d0d7f0122302b7357bf57e719399979b37a04e7bd8b13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animal reproduction</topic><topic>Animals</topic><topic>Artificial insemination</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Egg Yolk</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Epididymis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Ions</topic><topic>Male</topic><topic>Osmolar Concentration</topic><topic>Plasma</topic><topic>Polymers</topic><topic>Semen</topic><topic>Semen Preservation - methods</topic><topic>Semen Preservation - veterinary</topic><topic>Solutions</topic><topic>Sorbitol</topic><topic>Sperm Motility</topic><topic>Spermatozoa - physiology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Verberckmoes, S</creatorcontrib><creatorcontrib>Van Soom, A</creatorcontrib><creatorcontrib>Dewulf, J</creatorcontrib><creatorcontrib>De Pauw, I</creatorcontrib><creatorcontrib>De Kruif, A</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Reproduction in domestic animals</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Verberckmoes, S</au><au>Van Soom, A</au><au>Dewulf, J</au><au>De Pauw, I</au><au>De Kruif, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Storage of Fresh Bovine Semen in a Diluent Based on the Ionic Composition of Cauda Epididymal Plasma</atitle><jtitle>Reproduction in domestic animals</jtitle><addtitle>Reprod Domest Anim</addtitle><date>2004-12</date><risdate>2004</risdate><volume>39</volume><issue>6</issue><spage>410</spage><epage>416</epage><pages>410-416</pages><issn>0936-6768</issn><eissn>1439-0531</eissn><abstract>Contents
For artificial insemination (AI) in cattle, much lower insemination doses can be applied when fresh semen is used instead of frozen–thawed semen. However, a particular disadvantage of fresh semen is its limited shelf life. As bovine spermatozoa can be stored for several weeks in the cauda epididymis without negative effects on their fertilizing capacity, it is an interesting organ to serve as a model in order to prolong the shelf life of fresh semen. First, the storage capacity of a diluent [cauda epididymal plasma (CEP‐1)] with the same ionic composition, pH and osmolarity as the bovine CEP was compared with a Tris diluent for extended preservation of fresh ejaculated bovine semen. Secondly, the ionic composition of the CEP‐1 diluent was modified (CEP‐2) and its storage capacity was compared with this of the CEP‐1 and Tris diluent. Finally, the effect of addition of different polyols (sorbitol, glycerol, mannitol) and egg yolk concentrations (5, 10 and 20%) to the CEP‐2 diluent was assessed. Sperm quality decreased rapidly in the CEP‐1 diluent. The quality and especially progressive motility of spermatozoa stored in the CEP‐2 diluent were better those in the CEP‐1 and Tris diluent. No significant effects of different sugars or egg yolk concentrations on the quality of fresh bovine semen in the CEP‐2 diluent were observed. In conclusion, the CEP‐2 diluent with 10% egg yolk and 1 g/l sorbitol may be used for extended preservation of fresh bovine semen at 5°C up to 6 days.</abstract><cop>Berlin, Germany</cop><pub>Blackwell Verlag GmbH</pub><pmid>15598230</pmid><doi>10.1111/j.1439-0531.2004.00521.x</doi><tpages>7</tpages></addata></record> |
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| identifier | ISSN: 0936-6768 |
| ispartof | Reproduction in domestic animals, 2004-12, Vol.39 (6), p.410-416 |
| issn | 0936-6768 1439-0531 |
| language | eng |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animal reproduction Animals Artificial insemination Biological and medical sciences Cattle Egg Yolk Embryology: invertebrates and vertebrates. Teratology Epididymis Fundamental and applied biological sciences. Psychology Hydrogen-Ion Concentration Ions Male Osmolar Concentration Plasma Polymers Semen Semen Preservation - methods Semen Preservation - veterinary Solutions Sorbitol Sperm Motility Spermatozoa - physiology Time Factors |
| title | Storage of Fresh Bovine Semen in a Diluent Based on the Ionic Composition of Cauda Epididymal Plasma |
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