A high-yield and scaleable adenovirus vector production process based on high density perfusion culture of HEK 293 cells as suspended aggregates

Cells of the human embryonic kidney cell line (HEK 293) were grown as suspended aggregates in stirred vessels and infected with a recombinant adenovirus vector (Ad-TH-GFP). Regular spherical aggregates with the mean diameter less than 300 μm and a viable cell density greater than 5 × 10 6 cells·ml −...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Journal of bioscience and bioengineering 2009-05, Vol.107 (5), p.524-529
Hauptverfasser:	Liu, Hong, Liu, Xing-Mao, Li, Shi-Chong, Wu, Ben-Chuan, Ye, Ling-Ling, Wang, Qi-Wei, Chen, Zhao-Lie
Format:	Artikel
Sprache:	eng
Schlagworte:	ADENOVIRIDAE Adenoviridae - growth & development Adenoviridae - isolation & purification Adenovirus Aggregates Biological and medical sciences BIOREACTEUR BIOREACTORS BIORREACTORES Biotechnology CELL CULTURE Cell Culture Techniques - methods Cell Line CULTIVO DE CELULAS CULTURE DE CELLULE Fundamental and applied biological sciences. Psychology Gene therapy GENETIC TRANSFORMATION Genetic Vectors - isolation & purification Genetic Vectors - physiology HEK 293 cells Humans Kidney - physiology Kidney - virology Perfusion - methods Perfusion culture Production Recombinant adenoviral vector TERAPIA THERAPEUTIQUE THERAPY TRANSFORMACION GENETICA TRANSFORMATION GENETIQUE VACCINATION VACUNACION VECTEUR DE MALADIE VECTORES VECTORS Virus Cultivation - methods
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!