The stimulant cathartic, emodin, contracts the rat isolated ileum by triggering release of endogenous acetylcholine
Summary 1 Anthraquinone stimulant cathartics, such as emodin, are believed to increase the rate of contraction of ileum tissue in vitro via multiple mechanisms. The aim of this study was to probe the effects of emodin on acetylcholine (ACh)‐induced contraction of the rat isolated ileum preparation....
Gespeichert in:
| Veröffentlicht in: | Autonomic & autacoid pharmacology 2004-10, Vol.24 (4), p.103-105 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 105 |
|---|---|
| container_issue | 4 |
| container_start_page | 103 |
| container_title | Autonomic & autacoid pharmacology |
| container_volume | 24 |
| creator | Ali, S. Watson, M. S. Osborne, R. H. |
| description | Summary
1 Anthraquinone stimulant cathartics, such as emodin, are believed to increase the rate of contraction of ileum tissue in vitro via multiple mechanisms. The aim of this study was to probe the effects of emodin on acetylcholine (ACh)‐induced contraction of the rat isolated ileum preparation.
2 Ileal sections were incubated in Tyrode's solution and responses to methacholine, ACh and emodin obtained in the absence and presence of the muscarinic antagonist atropine and the choline uptake inhibitor hemicholinium (HC‐3). Depletion of endogenous ACh in the presence of HC‐3 was achieved by construction of an ACh dose–response curve, using exogenous ACh, prior to re‐testing the effects of emodin in the presence of HC‐3.
3 Emodin caused dose‐dependent tissue contraction that was abolished by inclusion of atropine (1 μm) in the buffer. Atropine (1 μm) antagonized the response caused by methacholine.
4 Incubation of tissues with HC‐3 (1 and 10 μm) reduced the maximum response caused by emodin by 45% and 71% respectively, but had no effect on ACh‐induced tissue contraction.
5 These data suggest that, emodin causes contraction of the ileum by triggering the release of endogenous ACh which acts on muscarinic receptors to cause contraction of the rat isolated ileum preparation. |
| doi_str_mv | 10.1111/j.1474-8673.2004.00321.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67179866</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67179866</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3851-f6059eae7874242a82a1fdb7715d11efc71a56d8c99cb4c407ddb0a5676d2c853</originalsourceid><addsrcrecordid>eNqNkF1v2yAUhtG0aW3T_YWJq17VLtjG2FJvoq5fUpV9KNV2hzAcJ6S2yQCryb8faaL0dtxwBM97ODwIYUpSGtfVKqUFL5Kq5HmaEVKkhOQZTTcf0Onx4uOxLtkJOvN-RQjlecE-oxPKWM3qrD5Ffr4E7IPpx04OASsZltIFoy4x9Fab4RIrOwQnVfA4RNTJgI23nQygselg7HGzxcGZxQKcGRbYQQfSA7YthkHbBQx29FgqCNtOLW1nBjhHn1rZefhy2Cfo-e52fvOQPH2_f7yZPiUqrxhN2pKwGiTwihdZkckqk7TVDeeUaUqhVZxKVupK1bVqClUQrnVD4hEvdaYqlk_Qxb7v2tm_I_ggeuMVdPGnEIcSJae8jnoiWO1B5az3DlqxdqaXbisoETvhYiV2LsXOq9gJF2_CxSZGvx7eGJse9HvwYDgC13vgNdra_ndjMZ3-iEWMJ_u48QE2x7h0L3H8nDPxe3Yv_vzi828_ORWz_B8CcKAP</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67179866</pqid></control><display><type>article</type><title>The stimulant cathartic, emodin, contracts the rat isolated ileum by triggering release of endogenous acetylcholine</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><creator>Ali, S. ; Watson, M. S. ; Osborne, R. H.</creator><creatorcontrib>Ali, S. ; Watson, M. S. ; Osborne, R. H.</creatorcontrib><description>Summary
1 Anthraquinone stimulant cathartics, such as emodin, are believed to increase the rate of contraction of ileum tissue in vitro via multiple mechanisms. The aim of this study was to probe the effects of emodin on acetylcholine (ACh)‐induced contraction of the rat isolated ileum preparation.
2 Ileal sections were incubated in Tyrode's solution and responses to methacholine, ACh and emodin obtained in the absence and presence of the muscarinic antagonist atropine and the choline uptake inhibitor hemicholinium (HC‐3). Depletion of endogenous ACh in the presence of HC‐3 was achieved by construction of an ACh dose–response curve, using exogenous ACh, prior to re‐testing the effects of emodin in the presence of HC‐3.
3 Emodin caused dose‐dependent tissue contraction that was abolished by inclusion of atropine (1 μm) in the buffer. Atropine (1 μm) antagonized the response caused by methacholine.
4 Incubation of tissues with HC‐3 (1 and 10 μm) reduced the maximum response caused by emodin by 45% and 71% respectively, but had no effect on ACh‐induced tissue contraction.
5 These data suggest that, emodin causes contraction of the ileum by triggering the release of endogenous ACh which acts on muscarinic receptors to cause contraction of the rat isolated ileum preparation.</description><identifier>ISSN: 1474-8665</identifier><identifier>EISSN: 1474-8673</identifier><identifier>DOI: 10.1111/j.1474-8673.2004.00321.x</identifier><identifier>PMID: 15595929</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>acetylcholine ; Acetylcholine - metabolism ; Animals ; Atropine - pharmacology ; Catecholamines - metabolism ; Cathartics - pharmacology ; Dose-Response Relationship, Drug ; emodin ; Emodin - antagonists & inhibitors ; Emodin - pharmacology ; Hemicholinium 3 - pharmacology ; ileum ; Ileum - drug effects ; Ileum - metabolism ; In Vitro Techniques ; Male ; Methacholine Chloride - pharmacology ; Muscarinic Agonists - pharmacology ; Muscarinic Antagonists - pharmacology ; Muscle Contraction - drug effects ; Muscle, Smooth - drug effects ; Muscle, Smooth - metabolism ; Neurotransmitter Uptake Inhibitors - pharmacology ; rat ; Rats ; Rats, Wistar</subject><ispartof>Autonomic & autacoid pharmacology, 2004-10, Vol.24 (4), p.103-105</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3851-f6059eae7874242a82a1fdb7715d11efc71a56d8c99cb4c407ddb0a5676d2c853</citedby><cites>FETCH-LOGICAL-c3851-f6059eae7874242a82a1fdb7715d11efc71a56d8c99cb4c407ddb0a5676d2c853</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1474-8673.2004.00321.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1474-8673.2004.00321.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15595929$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ali, S.</creatorcontrib><creatorcontrib>Watson, M. S.</creatorcontrib><creatorcontrib>Osborne, R. H.</creatorcontrib><title>The stimulant cathartic, emodin, contracts the rat isolated ileum by triggering release of endogenous acetylcholine</title><title>Autonomic & autacoid pharmacology</title><addtitle>Auton Autacoid Pharmacol</addtitle><description>Summary
1 Anthraquinone stimulant cathartics, such as emodin, are believed to increase the rate of contraction of ileum tissue in vitro via multiple mechanisms. The aim of this study was to probe the effects of emodin on acetylcholine (ACh)‐induced contraction of the rat isolated ileum preparation.
2 Ileal sections were incubated in Tyrode's solution and responses to methacholine, ACh and emodin obtained in the absence and presence of the muscarinic antagonist atropine and the choline uptake inhibitor hemicholinium (HC‐3). Depletion of endogenous ACh in the presence of HC‐3 was achieved by construction of an ACh dose–response curve, using exogenous ACh, prior to re‐testing the effects of emodin in the presence of HC‐3.
3 Emodin caused dose‐dependent tissue contraction that was abolished by inclusion of atropine (1 μm) in the buffer. Atropine (1 μm) antagonized the response caused by methacholine.
4 Incubation of tissues with HC‐3 (1 and 10 μm) reduced the maximum response caused by emodin by 45% and 71% respectively, but had no effect on ACh‐induced tissue contraction.
5 These data suggest that, emodin causes contraction of the ileum by triggering the release of endogenous ACh which acts on muscarinic receptors to cause contraction of the rat isolated ileum preparation.</description><subject>acetylcholine</subject><subject>Acetylcholine - metabolism</subject><subject>Animals</subject><subject>Atropine - pharmacology</subject><subject>Catecholamines - metabolism</subject><subject>Cathartics - pharmacology</subject><subject>Dose-Response Relationship, Drug</subject><subject>emodin</subject><subject>Emodin - antagonists & inhibitors</subject><subject>Emodin - pharmacology</subject><subject>Hemicholinium 3 - pharmacology</subject><subject>ileum</subject><subject>Ileum - drug effects</subject><subject>Ileum - metabolism</subject><subject>In Vitro Techniques</subject><subject>Male</subject><subject>Methacholine Chloride - pharmacology</subject><subject>Muscarinic Agonists - pharmacology</subject><subject>Muscarinic Antagonists - pharmacology</subject><subject>Muscle Contraction - drug effects</subject><subject>Muscle, Smooth - drug effects</subject><subject>Muscle, Smooth - metabolism</subject><subject>Neurotransmitter Uptake Inhibitors - pharmacology</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Wistar</subject><issn>1474-8665</issn><issn>1474-8673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF1v2yAUhtG0aW3T_YWJq17VLtjG2FJvoq5fUpV9KNV2hzAcJ6S2yQCryb8faaL0dtxwBM97ODwIYUpSGtfVKqUFL5Kq5HmaEVKkhOQZTTcf0Onx4uOxLtkJOvN-RQjlecE-oxPKWM3qrD5Ffr4E7IPpx04OASsZltIFoy4x9Fab4RIrOwQnVfA4RNTJgI23nQygselg7HGzxcGZxQKcGRbYQQfSA7YthkHbBQx29FgqCNtOLW1nBjhHn1rZefhy2Cfo-e52fvOQPH2_f7yZPiUqrxhN2pKwGiTwihdZkckqk7TVDeeUaUqhVZxKVupK1bVqClUQrnVD4hEvdaYqlk_Qxb7v2tm_I_ggeuMVdPGnEIcSJae8jnoiWO1B5az3DlqxdqaXbisoETvhYiV2LsXOq9gJF2_CxSZGvx7eGJse9HvwYDgC13vgNdra_ndjMZ3-iEWMJ_u48QE2x7h0L3H8nDPxe3Yv_vzi828_ORWz_B8CcKAP</recordid><startdate>200410</startdate><enddate>200410</enddate><creator>Ali, S.</creator><creator>Watson, M. S.</creator><creator>Osborne, R. H.</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200410</creationdate><title>The stimulant cathartic, emodin, contracts the rat isolated ileum by triggering release of endogenous acetylcholine</title><author>Ali, S. ; Watson, M. S. ; Osborne, R. H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3851-f6059eae7874242a82a1fdb7715d11efc71a56d8c99cb4c407ddb0a5676d2c853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>acetylcholine</topic><topic>Acetylcholine - metabolism</topic><topic>Animals</topic><topic>Atropine - pharmacology</topic><topic>Catecholamines - metabolism</topic><topic>Cathartics - pharmacology</topic><topic>Dose-Response Relationship, Drug</topic><topic>emodin</topic><topic>Emodin - antagonists & inhibitors</topic><topic>Emodin - pharmacology</topic><topic>Hemicholinium 3 - pharmacology</topic><topic>ileum</topic><topic>Ileum - drug effects</topic><topic>Ileum - metabolism</topic><topic>In Vitro Techniques</topic><topic>Male</topic><topic>Methacholine Chloride - pharmacology</topic><topic>Muscarinic Agonists - pharmacology</topic><topic>Muscarinic Antagonists - pharmacology</topic><topic>Muscle Contraction - drug effects</topic><topic>Muscle, Smooth - drug effects</topic><topic>Muscle, Smooth - metabolism</topic><topic>Neurotransmitter Uptake Inhibitors - pharmacology</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Wistar</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ali, S.</creatorcontrib><creatorcontrib>Watson, M. S.</creatorcontrib><creatorcontrib>Osborne, R. H.</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Autonomic & autacoid pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ali, S.</au><au>Watson, M. S.</au><au>Osborne, R. H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The stimulant cathartic, emodin, contracts the rat isolated ileum by triggering release of endogenous acetylcholine</atitle><jtitle>Autonomic & autacoid pharmacology</jtitle><addtitle>Auton Autacoid Pharmacol</addtitle><date>2004-10</date><risdate>2004</risdate><volume>24</volume><issue>4</issue><spage>103</spage><epage>105</epage><pages>103-105</pages><issn>1474-8665</issn><eissn>1474-8673</eissn><abstract>Summary
1 Anthraquinone stimulant cathartics, such as emodin, are believed to increase the rate of contraction of ileum tissue in vitro via multiple mechanisms. The aim of this study was to probe the effects of emodin on acetylcholine (ACh)‐induced contraction of the rat isolated ileum preparation.
2 Ileal sections were incubated in Tyrode's solution and responses to methacholine, ACh and emodin obtained in the absence and presence of the muscarinic antagonist atropine and the choline uptake inhibitor hemicholinium (HC‐3). Depletion of endogenous ACh in the presence of HC‐3 was achieved by construction of an ACh dose–response curve, using exogenous ACh, prior to re‐testing the effects of emodin in the presence of HC‐3.
3 Emodin caused dose‐dependent tissue contraction that was abolished by inclusion of atropine (1 μm) in the buffer. Atropine (1 μm) antagonized the response caused by methacholine.
4 Incubation of tissues with HC‐3 (1 and 10 μm) reduced the maximum response caused by emodin by 45% and 71% respectively, but had no effect on ACh‐induced tissue contraction.
5 These data suggest that, emodin causes contraction of the ileum by triggering the release of endogenous ACh which acts on muscarinic receptors to cause contraction of the rat isolated ileum preparation.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15595929</pmid><doi>10.1111/j.1474-8673.2004.00321.x</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1474-8665 |
| ispartof | Autonomic & autacoid pharmacology, 2004-10, Vol.24 (4), p.103-105 |
| issn | 1474-8665 1474-8673 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67179866 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | acetylcholine Acetylcholine - metabolism Animals Atropine - pharmacology Catecholamines - metabolism Cathartics - pharmacology Dose-Response Relationship, Drug emodin Emodin - antagonists & inhibitors Emodin - pharmacology Hemicholinium 3 - pharmacology ileum Ileum - drug effects Ileum - metabolism In Vitro Techniques Male Methacholine Chloride - pharmacology Muscarinic Agonists - pharmacology Muscarinic Antagonists - pharmacology Muscle Contraction - drug effects Muscle, Smooth - drug effects Muscle, Smooth - metabolism Neurotransmitter Uptake Inhibitors - pharmacology rat Rats Rats, Wistar |
| title | The stimulant cathartic, emodin, contracts the rat isolated ileum by triggering release of endogenous acetylcholine |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T02%3A30%3A26IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20stimulant%20cathartic,%20emodin,%20contracts%20the%20rat%20isolated%20ileum%20by%20triggering%20release%20of%20endogenous%20acetylcholine&rft.jtitle=Autonomic%20&%20autacoid%20pharmacology&rft.au=Ali,%20S.&rft.date=2004-10&rft.volume=24&rft.issue=4&rft.spage=103&rft.epage=105&rft.pages=103-105&rft.issn=1474-8665&rft.eissn=1474-8673&rft_id=info:doi/10.1111/j.1474-8673.2004.00321.x&rft_dat=%3Cproquest_cross%3E67179866%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67179866&rft_id=info:pmid/15595929&rfr_iscdi=true |