Immunohistochemical study on GABAergic system in salivary glands
Gamma-aminobutyric acid (GABA) and its receptors are found in the central nervous system and several peripheral tissues. The purpose of this study was to determine the expression and distribution of GABA and glutamate decarboxylase (GAD), a GABA biosynthetic enzyme, in rat salivary gland. Western bl...
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Veröffentlicht in: | European journal of pharmacology 2009-05, Vol.610 (1), p.18-22 |
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creator | Kosuge, Yasuhiro Kawaguchi, Mitsuru Sawaki, Kohei Okubo, Migiwa Shinomiya, Takashi Sakai, Takayuki |
description | Gamma-aminobutyric acid (GABA) and its receptors are found in the central nervous system and several peripheral tissues. The purpose of this study was to determine the expression and distribution of GABA and glutamate decarboxylase (GAD), a GABA biosynthetic enzyme, in rat salivary gland. Western blot and real time quantitative RT-PCR revealed that GAD67 was the major isoform of GAD in the salivary glands. Furthermore, both GABA and GAD were detected around the acinar cells in the submandibular glands by immunohistochemical analysis. When both sympathetic and parasympathetic nerves related to the submandibular glands were denervated, the immunoreactivities of GABA and GAD were dramatically depressed, and levels of GAD67 and GABA significantly decreased. However, no morphological changes in the glands were observed after denervation. These results indicate that GAD67 is present around acinar cells in the salivary glands, and suggest that the GABAergic system in the glands is closely related to the autonomic nervous system. |
doi_str_mv | 10.1016/j.ejphar.2009.02.043 |
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The purpose of this study was to determine the expression and distribution of GABA and glutamate decarboxylase (GAD), a GABA biosynthetic enzyme, in rat salivary gland. Western blot and real time quantitative RT-PCR revealed that GAD67 was the major isoform of GAD in the salivary glands. Furthermore, both GABA and GAD were detected around the acinar cells in the submandibular glands by immunohistochemical analysis. When both sympathetic and parasympathetic nerves related to the submandibular glands were denervated, the immunoreactivities of GABA and GAD were dramatically depressed, and levels of GAD67 and GABA significantly decreased. However, no morphological changes in the glands were observed after denervation. These results indicate that GAD67 is present around acinar cells in the salivary glands, and suggest that the GABAergic system in the glands is closely related to the autonomic nervous system.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/j.ejphar.2009.02.043</identifier><identifier>PMID: 19292982</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Autonomic Nervous System - metabolism ; Biological and medical sciences ; Gamma-aminobutyric acid ; gamma-Aminobutyric Acid - genetics ; gamma-Aminobutyric Acid - metabolism ; Gene Expression ; Glutamate decarboxylase ; Glutamate Decarboxylase - analysis ; Glutamate Decarboxylase - genetics ; Glutamate Decarboxylase - metabolism ; Immunohistochemistry ; Innervation ; Isoenzymes - analysis ; Isoenzymes - genetics ; Isoenzymes - metabolism ; Male ; Medical sciences ; Peripheral nervous system ; Pharmacology. Drug treatments ; Rats ; Rats, Wistar ; Salivary gland ; Salivary Glands - metabolism ; Submandibular Gland - enzymology ; Submandibular Gland - metabolism ; Tissue Distribution</subject><ispartof>European journal of pharmacology, 2009-05, Vol.610 (1), p.18-22</ispartof><rights>2009 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c546t-b9233b07e13ffaebc074c0d57253fd50c47004c4723d110fd4c45adc5d10585b3</citedby><cites>FETCH-LOGICAL-c546t-b9233b07e13ffaebc074c0d57253fd50c47004c4723d110fd4c45adc5d10585b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ejphar.2009.02.043$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21500676$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19292982$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kosuge, Yasuhiro</creatorcontrib><creatorcontrib>Kawaguchi, Mitsuru</creatorcontrib><creatorcontrib>Sawaki, Kohei</creatorcontrib><creatorcontrib>Okubo, Migiwa</creatorcontrib><creatorcontrib>Shinomiya, Takashi</creatorcontrib><creatorcontrib>Sakai, Takayuki</creatorcontrib><title>Immunohistochemical study on GABAergic system in salivary glands</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>Gamma-aminobutyric acid (GABA) and its receptors are found in the central nervous system and several peripheral tissues. The purpose of this study was to determine the expression and distribution of GABA and glutamate decarboxylase (GAD), a GABA biosynthetic enzyme, in rat salivary gland. Western blot and real time quantitative RT-PCR revealed that GAD67 was the major isoform of GAD in the salivary glands. Furthermore, both GABA and GAD were detected around the acinar cells in the submandibular glands by immunohistochemical analysis. When both sympathetic and parasympathetic nerves related to the submandibular glands were denervated, the immunoreactivities of GABA and GAD were dramatically depressed, and levels of GAD67 and GABA significantly decreased. However, no morphological changes in the glands were observed after denervation. These results indicate that GAD67 is present around acinar cells in the salivary glands, and suggest that the GABAergic system in the glands is closely related to the autonomic nervous system.</description><subject>Animals</subject><subject>Autonomic Nervous System - metabolism</subject><subject>Biological and medical sciences</subject><subject>Gamma-aminobutyric acid</subject><subject>gamma-Aminobutyric Acid - genetics</subject><subject>gamma-Aminobutyric Acid - metabolism</subject><subject>Gene Expression</subject><subject>Glutamate decarboxylase</subject><subject>Glutamate Decarboxylase - analysis</subject><subject>Glutamate Decarboxylase - genetics</subject><subject>Glutamate Decarboxylase - metabolism</subject><subject>Immunohistochemistry</subject><subject>Innervation</subject><subject>Isoenzymes - analysis</subject><subject>Isoenzymes - genetics</subject><subject>Isoenzymes - metabolism</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Peripheral nervous system</subject><subject>Pharmacology. Drug treatments</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Salivary gland</subject><subject>Salivary Glands - metabolism</subject><subject>Submandibular Gland - enzymology</subject><subject>Submandibular Gland - metabolism</subject><subject>Tissue Distribution</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1P4zAQhq0VaOl29x-sUC5wSxg7cdxcEAUtUAmJC3u2HHtCXeWjeJJK_fcYtYIbGmlmDs-MXj2M_eWQceDl1SbDzXZtQiYAqgxEBkX-g834QlUpKC5O2AyAF6moquqM_SLaAICshPzJznglYi3EjN2sum7qh7WncbBr7Lw1bULj5PbJ0CcPy9slhldvE9rTiF3i-4RM63cm7JPX1vSOfrPTxrSEf45zzv7f_3u5e0yfnh9Wd8un1MqiHNO6Enleg0KeN43B2oIqLDiphMwbJ8EWCqCIXeSOc2hc3KVxVjoOciHrfM4uD3-3YXibkEbdebLYxhA4TKRLxZXMpYpgcQBtGIgCNnobfBcDaw76w5ze6IM5_WFOg9DRXDw7P_6f6g7d19FRVQQujoChKKkJpreePjnBJUCpyshdHziMNnYegybrsbfofEA7ajf475O8A_gMjZo</recordid><startdate>20090521</startdate><enddate>20090521</enddate><creator>Kosuge, Yasuhiro</creator><creator>Kawaguchi, Mitsuru</creator><creator>Sawaki, Kohei</creator><creator>Okubo, Migiwa</creator><creator>Shinomiya, Takashi</creator><creator>Sakai, Takayuki</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090521</creationdate><title>Immunohistochemical study on GABAergic system in salivary glands</title><author>Kosuge, Yasuhiro ; Kawaguchi, Mitsuru ; Sawaki, Kohei ; Okubo, Migiwa ; Shinomiya, Takashi ; Sakai, Takayuki</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c546t-b9233b07e13ffaebc074c0d57253fd50c47004c4723d110fd4c45adc5d10585b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Autonomic Nervous System - metabolism</topic><topic>Biological and medical sciences</topic><topic>Gamma-aminobutyric acid</topic><topic>gamma-Aminobutyric Acid - genetics</topic><topic>gamma-Aminobutyric Acid - metabolism</topic><topic>Gene Expression</topic><topic>Glutamate decarboxylase</topic><topic>Glutamate Decarboxylase - analysis</topic><topic>Glutamate Decarboxylase - genetics</topic><topic>Glutamate Decarboxylase - metabolism</topic><topic>Immunohistochemistry</topic><topic>Innervation</topic><topic>Isoenzymes - analysis</topic><topic>Isoenzymes - genetics</topic><topic>Isoenzymes - metabolism</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Peripheral nervous system</topic><topic>Pharmacology. Drug treatments</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Salivary gland</topic><topic>Salivary Glands - metabolism</topic><topic>Submandibular Gland - enzymology</topic><topic>Submandibular Gland - metabolism</topic><topic>Tissue Distribution</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kosuge, Yasuhiro</creatorcontrib><creatorcontrib>Kawaguchi, Mitsuru</creatorcontrib><creatorcontrib>Sawaki, Kohei</creatorcontrib><creatorcontrib>Okubo, Migiwa</creatorcontrib><creatorcontrib>Shinomiya, Takashi</creatorcontrib><creatorcontrib>Sakai, Takayuki</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kosuge, Yasuhiro</au><au>Kawaguchi, Mitsuru</au><au>Sawaki, Kohei</au><au>Okubo, Migiwa</au><au>Shinomiya, Takashi</au><au>Sakai, Takayuki</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunohistochemical study on GABAergic system in salivary glands</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>2009-05-21</date><risdate>2009</risdate><volume>610</volume><issue>1</issue><spage>18</spage><epage>22</epage><pages>18-22</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>Gamma-aminobutyric acid (GABA) and its receptors are found in the central nervous system and several peripheral tissues. The purpose of this study was to determine the expression and distribution of GABA and glutamate decarboxylase (GAD), a GABA biosynthetic enzyme, in rat salivary gland. Western blot and real time quantitative RT-PCR revealed that GAD67 was the major isoform of GAD in the salivary glands. Furthermore, both GABA and GAD were detected around the acinar cells in the submandibular glands by immunohistochemical analysis. When both sympathetic and parasympathetic nerves related to the submandibular glands were denervated, the immunoreactivities of GABA and GAD were dramatically depressed, and levels of GAD67 and GABA significantly decreased. However, no morphological changes in the glands were observed after denervation. These results indicate that GAD67 is present around acinar cells in the salivary glands, and suggest that the GABAergic system in the glands is closely related to the autonomic nervous system.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19292982</pmid><doi>10.1016/j.ejphar.2009.02.043</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Autonomic Nervous System - metabolism Biological and medical sciences Gamma-aminobutyric acid gamma-Aminobutyric Acid - genetics gamma-Aminobutyric Acid - metabolism Gene Expression Glutamate decarboxylase Glutamate Decarboxylase - analysis Glutamate Decarboxylase - genetics Glutamate Decarboxylase - metabolism Immunohistochemistry Innervation Isoenzymes - analysis Isoenzymes - genetics Isoenzymes - metabolism Male Medical sciences Peripheral nervous system Pharmacology. Drug treatments Rats Rats, Wistar Salivary gland Salivary Glands - metabolism Submandibular Gland - enzymology Submandibular Gland - metabolism Tissue Distribution |
title | Immunohistochemical study on GABAergic system in salivary glands |
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