Immunohistochemical study on GABAergic system in salivary glands

Gamma-aminobutyric acid (GABA) and its receptors are found in the central nervous system and several peripheral tissues. The purpose of this study was to determine the expression and distribution of GABA and glutamate decarboxylase (GAD), a GABA biosynthetic enzyme, in rat salivary gland. Western bl...

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Veröffentlicht in:European journal of pharmacology 2009-05, Vol.610 (1), p.18-22
Hauptverfasser: Kosuge, Yasuhiro, Kawaguchi, Mitsuru, Sawaki, Kohei, Okubo, Migiwa, Shinomiya, Takashi, Sakai, Takayuki
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container_title European journal of pharmacology
container_volume 610
creator Kosuge, Yasuhiro
Kawaguchi, Mitsuru
Sawaki, Kohei
Okubo, Migiwa
Shinomiya, Takashi
Sakai, Takayuki
description Gamma-aminobutyric acid (GABA) and its receptors are found in the central nervous system and several peripheral tissues. The purpose of this study was to determine the expression and distribution of GABA and glutamate decarboxylase (GAD), a GABA biosynthetic enzyme, in rat salivary gland. Western blot and real time quantitative RT-PCR revealed that GAD67 was the major isoform of GAD in the salivary glands. Furthermore, both GABA and GAD were detected around the acinar cells in the submandibular glands by immunohistochemical analysis. When both sympathetic and parasympathetic nerves related to the submandibular glands were denervated, the immunoreactivities of GABA and GAD were dramatically depressed, and levels of GAD67 and GABA significantly decreased. However, no morphological changes in the glands were observed after denervation. These results indicate that GAD67 is present around acinar cells in the salivary glands, and suggest that the GABAergic system in the glands is closely related to the autonomic nervous system.
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subjects Animals
Autonomic Nervous System - metabolism
Biological and medical sciences
Gamma-aminobutyric acid
gamma-Aminobutyric Acid - genetics
gamma-Aminobutyric Acid - metabolism
Gene Expression
Glutamate decarboxylase
Glutamate Decarboxylase - analysis
Glutamate Decarboxylase - genetics
Glutamate Decarboxylase - metabolism
Immunohistochemistry
Innervation
Isoenzymes - analysis
Isoenzymes - genetics
Isoenzymes - metabolism
Male
Medical sciences
Peripheral nervous system
Pharmacology. Drug treatments
Rats
Rats, Wistar
Salivary gland
Salivary Glands - metabolism
Submandibular Gland - enzymology
Submandibular Gland - metabolism
Tissue Distribution
title Immunohistochemical study on GABAergic system in salivary glands
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