Skeletal Muscle Aging in F344BN F1-Hybrid Rats: I. Mitochondrial Dysfunction Contributes to the Age-Associated Reduction in VO2max

Although mitochondrial DNA damage accumulates in aging skeletal muscles, how this relates to the decline in muscle mass-specific skeletal muscle aerobic function is unknown. We used a pump-perfused rat hind-limb model to examine maximal aerobic performance (V̇O2max) in young adult (YA; 8–9-month-old...

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Veröffentlicht in:The journals of gerontology. Series A, Biological sciences and medical sciences Biological sciences and medical sciences, 2004-11, Vol.59 (11), p.1099-1110
Hauptverfasser: Hagen, Jason L., Krause, Daniel J., Baker, David J., Fu, Ming Hua, Tarnopolsky, Mark A., Hepple, Russell T.
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container_issue 11
container_start_page 1099
container_title The journals of gerontology. Series A, Biological sciences and medical sciences
container_volume 59
creator Hagen, Jason L.
Krause, Daniel J.
Baker, David J.
Fu, Ming Hua
Tarnopolsky, Mark A.
Hepple, Russell T.
description Although mitochondrial DNA damage accumulates in aging skeletal muscles, how this relates to the decline in muscle mass-specific skeletal muscle aerobic function is unknown. We used a pump-perfused rat hind-limb model to examine maximal aerobic performance (V̇O2max) in young adult (YA; 8–9-month-old), late middle aged (LMA; 28–30-month-old) and senescent (SEN; 36-month-old) Fischer 344 × Brown Norway F1-hybrid rats at matched rates of convective O2 delivery (QO2). Despite similar muscle QO2 during a 4-minute contraction bout, muscle mass-specific V̇O2max was reduced in LMA (15%) and SEN (52%) versus YA. In plantaris muscle homogenates, nested polymerase chain reaction revealed an increased frequency of mitochondrial DNA deletions in the older animals. A greater reduction in the flux through electron transport chain complexes I–III than citrate synthase activity in the older animals suggests mitochondrial dysfunction consequent to mitochondrial DNA damage with aging. These results support the hypothesis that a reduced oxidative capacity, due in part to age-related mitochondrial dysfunction, contributes to the decline in aerobic performance in aging skeletal muscles.
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source MEDLINE; Oxford University Press Journals All Titles (1996-Current)
subjects Aerobiosis
Aging - metabolism
Animals
DNA Damage
DNA, Mitochondrial - genetics
Electron Transport
Hindlimb
Male
Mitochondria, Muscle - metabolism
Muscle Contraction
Muscle, Skeletal - blood supply
Muscle, Skeletal - metabolism
Muscle, Skeletal - physiology
Oxygen Consumption
Rats
Rats, Inbred BN
Rats, Inbred F344
Sequence Deletion
title Skeletal Muscle Aging in F344BN F1-Hybrid Rats: I. Mitochondrial Dysfunction Contributes to the Age-Associated Reduction in VO2max
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