Morphological quality and nucleic acid preservation in cytopathology
Background:Fixation is a chemical or physical procedure to prevent the degradation of proteins and tissue morphology. To optimise molecular analysis of archival tissues, it is essential that fixation preserves morphology along with protein epitopes and DNA/RNA integrity.Methods:A new formalin-free a...
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| Veröffentlicht in: | Journal of clinical pathology 2009-05, Vol.62 (5), p.429-434 |
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| creator | Gazziero, A Guzzardo, V Aldighieri, E Fassina, A |
| description | Background:Fixation is a chemical or physical procedure to prevent the degradation of proteins and tissue morphology. To optimise molecular analysis of archival tissues, it is essential that fixation preserves morphology along with protein epitopes and DNA/RNA integrity.Methods:A new formalin-free alcoholic-based fixative, FineFIX, was used to fix 15 serous effusions and 38 fine-needle aspirates, and cellular morphology and nucleic acid quality were evaluated.Results:The cytomorphology of the effusions and fine-needle aspirates obtained with FineFIX fixation was similar to that obtained with formalin-fixed counterparts. Immunocytochemistry showed comparable results with the traditional fixative, but FineFIX preserved higher-molecular-mass DNA and RNA, as demonstrated by successful PCR of large amplification products of >2000 bp.Conclusions:The formalin-free fixative produced not only satisfactory results for immunocytochemistry on cytological smears and cell blocks, but also excellent preservation of DNA and RNA, which can also be efficiently used for sophisticated molecular techniques. |
| doi_str_mv | 10.1136/jcp.2008.059808 |
| format | Article |
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To optimise molecular analysis of archival tissues, it is essential that fixation preserves morphology along with protein epitopes and DNA/RNA integrity.Methods:A new formalin-free alcoholic-based fixative, FineFIX, was used to fix 15 serous effusions and 38 fine-needle aspirates, and cellular morphology and nucleic acid quality were evaluated.Results:The cytomorphology of the effusions and fine-needle aspirates obtained with FineFIX fixation was similar to that obtained with formalin-fixed counterparts. Immunocytochemistry showed comparable results with the traditional fixative, but FineFIX preserved higher-molecular-mass DNA and RNA, as demonstrated by successful PCR of large amplification products of >2000 bp.Conclusions:The formalin-free fixative produced not only satisfactory results for immunocytochemistry on cytological smears and cell blocks, but also excellent preservation of DNA and RNA, which can also be efficiently used for sophisticated molecular techniques.</description><identifier>ISSN: 0021-9746</identifier><identifier>EISSN: 1472-4146</identifier><identifier>DOI: 10.1136/jcp.2008.059808</identifier><identifier>PMID: 18818264</identifier><identifier>CODEN: JCPAAK</identifier><language>eng</language><publisher>London: BMJ Publishing Group Ltd and Association of Clinical Pathologists</publisher><subject>Biological and medical sciences ; Biopsy, Fine-Needle ; DNA - analysis ; Fixatives - pharmacology ; Formaldehyde ; Humans ; Immunoenzyme Techniques ; Investigative techniques, diagnostic techniques (general aspects) ; Medical sciences ; Pathology, Clinical - methods ; Pathology. 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Immunocytochemistry showed comparable results with the traditional fixative, but FineFIX preserved higher-molecular-mass DNA and RNA, as demonstrated by successful PCR of large amplification products of >2000 bp.Conclusions:The formalin-free fixative produced not only satisfactory results for immunocytochemistry on cytological smears and cell blocks, but also excellent preservation of DNA and RNA, which can also be efficiently used for sophisticated molecular techniques.</description><subject>Biological and medical sciences</subject><subject>Biopsy, Fine-Needle</subject><subject>DNA - analysis</subject><subject>Fixatives - pharmacology</subject><subject>Formaldehyde</subject><subject>Humans</subject><subject>Immunoenzyme Techniques</subject><subject>Investigative techniques, diagnostic techniques (general aspects)</subject><subject>Medical sciences</subject><subject>Pathology, Clinical - methods</subject><subject>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA - analysis</subject><subject>Tissue Fixation - methods</subject><issn>0021-9746</issn><issn>1472-4146</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkc9PHCEYhknTpq62Z29mkqYemszKBwwwx2bVWrVt0l9XwgCrbGeHEWaM-9-XdTaa9FIuHL7ne_PygNAh4DkA5Scr088JxnKOq1pi-QLNgAlSMmD8JZphTKCsBeN7aD-lFcZABdDXaA-kBEk4m6HTLyH2t6ENN97otrgbdeuHTaE7W3SjaZ03hTbeFn10ycV7PfjQFb4rzGYIvR4eNzdv0KulbpN7u7sP0K_zs5-Li_L626fPi4_XZcMIH0oBjFoHWFhOmqVlBiwXjnEJ1NVAK9pYUkHtdCPzYUazPLCEScqJoLaiB-h4yu1juBtdGtTaJ-PaVncujElxAQJqUWfw3T_gKoyxy90UCAmYVpLLTJ1MlIkhpeiWqo9-reNGAVZbvSrrVVu9atKbN452uWOzdvaZ3_nMwPsdoFP2uYy6Mz49cSR_QMXrbcFy4nwa3MPTXMc_-RGZUV9_L9TV5en3K_FDqG3uh4lv1qv_tvwLlJmdyw</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>Gazziero, A</creator><creator>Guzzardo, V</creator><creator>Aldighieri, E</creator><creator>Fassina, A</creator><general>BMJ Publishing Group Ltd and Association of Clinical Pathologists</general><general>BMJ Publishing Group</general><general>BMJ Publishing Group LTD</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8AF</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>BTHHO</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20090501</creationdate><title>Morphological quality and nucleic acid preservation in cytopathology</title><author>Gazziero, A ; Guzzardo, V ; Aldighieri, E ; Fassina, A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b426t-7143de107d62bfd4c1d67e46813e91353bd2519eab88884ca413ed24836273d53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biological and medical sciences</topic><topic>Biopsy, Fine-Needle</topic><topic>DNA - analysis</topic><topic>Fixatives - pharmacology</topic><topic>Formaldehyde</topic><topic>Humans</topic><topic>Immunoenzyme Techniques</topic><topic>Investigative techniques, diagnostic techniques (general aspects)</topic><topic>Medical sciences</topic><topic>Pathology, Clinical - methods</topic><topic>Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RNA - analysis</topic><topic>Tissue Fixation - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gazziero, A</creatorcontrib><creatorcontrib>Guzzardo, V</creatorcontrib><creatorcontrib>Aldighieri, E</creatorcontrib><creatorcontrib>Fassina, A</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>STEM Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>BMJ Journals</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of clinical pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gazziero, A</au><au>Guzzardo, V</au><au>Aldighieri, E</au><au>Fassina, A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Morphological quality and nucleic acid preservation in cytopathology</atitle><jtitle>Journal of clinical pathology</jtitle><addtitle>J Clin Pathol</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>62</volume><issue>5</issue><spage>429</spage><epage>434</epage><pages>429-434</pages><issn>0021-9746</issn><eissn>1472-4146</eissn><coden>JCPAAK</coden><abstract>Background:Fixation is a chemical or physical procedure to prevent the degradation of proteins and tissue morphology. 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| subjects | Biological and medical sciences Biopsy, Fine-Needle DNA - analysis Fixatives - pharmacology Formaldehyde Humans Immunoenzyme Techniques Investigative techniques, diagnostic techniques (general aspects) Medical sciences Pathology, Clinical - methods Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques Reverse Transcriptase Polymerase Chain Reaction - methods RNA - analysis Tissue Fixation - methods |
| title | Morphological quality and nucleic acid preservation in cytopathology |
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