Alternative splicing of mRNA of mouse interleukin-4 and interleukin-6
Interleukin-4 and interleukin-6 are multifunctional regulatory proteins, which participate both in haemopoiesis and in immunopoiesis. The alternative splicing of these interleukins in humans is known to proceed in a tissue-specific manner. Additionally, changes in splicing can also be dependent on t...
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| Veröffentlicht in: | Cytokine (Philadelphia, Pa.) Pa.), 2004-11, Vol.28 (4), p.190-196 |
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| creator | Yatsenko, Olga P. Filipenko, Maxim L. Khrapov, Eugene A. Voronina, Elena N. Kozlov, Vladimir A. Sennikov, Sergey V. |
| description | Interleukin-4 and interleukin-6 are multifunctional regulatory proteins, which participate both in haemopoiesis and in immunopoiesis. The alternative splicing of these interleukins in humans is known to proceed in a tissue-specific manner. Additionally, changes in splicing can also be dependent on tissue pathology.
In this work, we report on the presence of alternatively spliced mRNA (IL-4δ2mRNA), lacking exon 2, in mouse bone marrow and spleen cells. We find that in unstimulated cells IL-4mRNA levels strongly dominate over IL-4δ2mRNA levels. Both increase in response to stimulation, with the concentration of the alternative variant rising earlier and faster than that of the full-length variant. In all other tissues studied dominance of IL-4δ2mRNA over the full-length variant was not observed.
In addition, we find expression of three forms of IL-6 mRNA: the full-length IL-6 mRNA, IL-6Δ3 mRNA, and IL-6Δ5 mRNA in the second and third trimester placenta tissue and in the spleen of mice immunized with a high dose of sheep erythrocytes. It is anticipated that translation of these mRNA variants can generate proteins capable of binding to some subunits of the IL-6 receptor, thus possessing effector function.
Alternative splicing is discussed as a source of cytokines with new regulatory properties. |
| doi_str_mv | 10.1016/j.cyto.2004.08.009 |
| format | Article |
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In this work, we report on the presence of alternatively spliced mRNA (IL-4δ2mRNA), lacking exon 2, in mouse bone marrow and spleen cells. We find that in unstimulated cells IL-4mRNA levels strongly dominate over IL-4δ2mRNA levels. Both increase in response to stimulation, with the concentration of the alternative variant rising earlier and faster than that of the full-length variant. In all other tissues studied dominance of IL-4δ2mRNA over the full-length variant was not observed.
In addition, we find expression of three forms of IL-6 mRNA: the full-length IL-6 mRNA, IL-6Δ3 mRNA, and IL-6Δ5 mRNA in the second and third trimester placenta tissue and in the spleen of mice immunized with a high dose of sheep erythrocytes. It is anticipated that translation of these mRNA variants can generate proteins capable of binding to some subunits of the IL-6 receptor, thus possessing effector function.
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In this work, we report on the presence of alternatively spliced mRNA (IL-4δ2mRNA), lacking exon 2, in mouse bone marrow and spleen cells. We find that in unstimulated cells IL-4mRNA levels strongly dominate over IL-4δ2mRNA levels. Both increase in response to stimulation, with the concentration of the alternative variant rising earlier and faster than that of the full-length variant. In all other tissues studied dominance of IL-4δ2mRNA over the full-length variant was not observed.
In addition, we find expression of three forms of IL-6 mRNA: the full-length IL-6 mRNA, IL-6Δ3 mRNA, and IL-6Δ5 mRNA in the second and third trimester placenta tissue and in the spleen of mice immunized with a high dose of sheep erythrocytes. It is anticipated that translation of these mRNA variants can generate proteins capable of binding to some subunits of the IL-6 receptor, thus possessing effector function.
Alternative splicing is discussed as a source of cytokines with new regulatory properties.</description><subject>Alternative splicing</subject><subject>Alternative Splicing - genetics</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Concanavalin A - pharmacology</subject><subject>Exons - genetics</subject><subject>Female</subject><subject>Gene Expression Profiling</subject><subject>Humans</subject><subject>IL-4</subject><subject>IL-6</subject><subject>Interleukin-4 - genetics</subject><subject>Interleukin-6 - chemistry</subject><subject>Interleukin-6 - genetics</subject><subject>Isoform</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Protein Isoforms - chemistry</subject><subject>Protein Isoforms - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><issn>1043-4666</issn><issn>1096-0023</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkF1LwzAYhYMobk7_gBfSK-9a3zRtmoA3Y8wPGAqi16FN3kpmP2bTDvbvTd1AvBGvTgjPOSQPIZcUIgqU36wjvevbKAZIIhARgDwiUwqShwAxOx7PCQsTzvmEnDm3Bk-wLDslE5qmQnDJp2Q5r3rsmry3WwzcprLaNu9BWwb1y9P8O9vBYWAbT1U4fNgmTIK8Mb9u-Dk5KfPK4cUhZ-Ttbvm6eAhXz_ePi_kq1EywPkylKZAyzCU1oCHLSx-C5ah5SmWc8gJ0bDJuZCFZmWSUxyiQSd-m_scZm5Hr_e6maz8HdL2qrdNYVXmD_p2K-4oU_wBpJgQkceLBeA_qrnWuw1JtOlvn3U5RUKNltVajZTVaViDU6HBGrg7rQ1Gj-akctHrgdg-gl7G12CmnLTYaje1Q98q09q_9L0qPjQ8</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Yatsenko, Olga P.</creator><creator>Filipenko, Maxim L.</creator><creator>Khrapov, Eugene A.</creator><creator>Voronina, Elena N.</creator><creator>Kozlov, Vladimir A.</creator><creator>Sennikov, Sergey V.</creator><general>Elsevier Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Alternative splicing of mRNA of mouse interleukin-4 and interleukin-6</title><author>Yatsenko, Olga P. ; 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The alternative splicing of these interleukins in humans is known to proceed in a tissue-specific manner. Additionally, changes in splicing can also be dependent on tissue pathology.
In this work, we report on the presence of alternatively spliced mRNA (IL-4δ2mRNA), lacking exon 2, in mouse bone marrow and spleen cells. We find that in unstimulated cells IL-4mRNA levels strongly dominate over IL-4δ2mRNA levels. Both increase in response to stimulation, with the concentration of the alternative variant rising earlier and faster than that of the full-length variant. In all other tissues studied dominance of IL-4δ2mRNA over the full-length variant was not observed.
In addition, we find expression of three forms of IL-6 mRNA: the full-length IL-6 mRNA, IL-6Δ3 mRNA, and IL-6Δ5 mRNA in the second and third trimester placenta tissue and in the spleen of mice immunized with a high dose of sheep erythrocytes. It is anticipated that translation of these mRNA variants can generate proteins capable of binding to some subunits of the IL-6 receptor, thus possessing effector function.
Alternative splicing is discussed as a source of cytokines with new regulatory properties.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>15588696</pmid><doi>10.1016/j.cyto.2004.08.009</doi><tpages>7</tpages></addata></record> |
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| ispartof | Cytokine (Philadelphia, Pa.), 2004-11, Vol.28 (4), p.190-196 |
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| subjects | Alternative splicing Alternative Splicing - genetics Amino Acid Sequence Animals Concanavalin A - pharmacology Exons - genetics Female Gene Expression Profiling Humans IL-4 IL-6 Interleukin-4 - genetics Interleukin-6 - chemistry Interleukin-6 - genetics Isoform Male Mice Mice, Inbred C57BL Molecular Sequence Data Polymerase Chain Reaction Protein Isoforms - chemistry Protein Isoforms - genetics RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Homology, Amino Acid |
| title | Alternative splicing of mRNA of mouse interleukin-4 and interleukin-6 |
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