Monoclonal antibodies preventing invasion of Neospora caninum tachyzoites into host cells
Twelve monoclonal antibodies (MAbs) against Neospora caninum tachyzoites were produced to specify the antigens related to the invasion of tachyzoites into host cells. In the assay to evaluate the inhibition activity, all these MAbS prevented the cultured Vero cells from the invading by the tachyzoit...
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| Veröffentlicht in: | Journal of Veterinary Medical Science 2004, Vol.66(11), pp.1355-1358 |
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| container_issue | 11 |
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| container_title | Journal of Veterinary Medical Science |
| container_volume | 66 |
| creator | Uchida, Y. (Nippon Veterinary and Animal Science Univ., Musashino, Tokyo (Japan)) Ike, K Kurotaki, T Ito, A Imai, S |
| description | Twelve monoclonal antibodies (MAbs) against Neospora caninum tachyzoites were produced to specify the antigens related to the invasion of tachyzoites into host cells. In the assay to evaluate the inhibition activity, all these MAbS prevented the cultured Vero cells from the invading by the tachyzoites. These MAbs recognized approximately a 73 kDa antigen in Western blot analysis. Immunofluorescence assay and immune electron microscopy revealed that this 73 kDa antigen is a part of the surface antigens of N. caninum tachyzoite, and that the tachyzoite antigen identified plays an important role for invasion of host cells. |
| doi_str_mv | 10.1292/jvms.66.1355 |
| format | Article |
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(Nippon Veterinary and Animal Science Univ., Musashino, Tokyo (Japan)) ; Ike, K ; Kurotaki, T ; Ito, A ; Imai, S</creator><creatorcontrib>Uchida, Y. (Nippon Veterinary and Animal Science Univ., Musashino, Tokyo (Japan)) ; Ike, K ; Kurotaki, T ; Ito, A ; Imai, S</creatorcontrib><description>Twelve monoclonal antibodies (MAbs) against Neospora caninum tachyzoites were produced to specify the antigens related to the invasion of tachyzoites into host cells. In the assay to evaluate the inhibition activity, all these MAbS prevented the cultured Vero cells from the invading by the tachyzoites. These MAbs recognized approximately a 73 kDa antigen in Western blot analysis. 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Immunofluorescence assay and immune electron microscopy revealed that this 73 kDa antigen is a part of the surface antigens of N. caninum tachyzoite, and that the tachyzoite antigen identified plays an important role for invasion of host cells.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antigens, Protozoan</subject><subject>CELLS</subject><subject>Cercopithecus aethiops</subject><subject>COWS</subject><subject>DOGS</subject><subject>Female</subject><subject>HOST PARASITE RELATIONS</subject><subject>invasion process</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>monoclonal antibody</subject><subject>Neospora - immunology</subject><subject>Neospora - pathogenicity</subject><subject>NEOSPORA CANINUM</subject><subject>surface antigen</subject><subject>Vero Cells</subject><issn>0916-7250</issn><issn>1347-7439</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkcuLFDEQh4Mo7uzqzavSIOzJHvPu5CiDT9bHQQ-eQnU6PZOhO5lN0gPrX283M-yCFy8pQn31UdQPoRcErwnV9O3-OOa1lGvChHiEVoTxpm4404_RCmsi64YKfIEuc95jTAmX-im6IEIooblaod9fY4h2iAGGCkLxbey8y9UhuaObv2Fb-XCE7GOoYl99czEfYoLKQvBhGqsCdnf3J_oyz_hQYrWLuVTWDUN-hp70MGT3_Fyv0K8P739uPtU33z9-3ry7qe28RKmhFS3VTluluO6hl71gXAgipHKSWi551zFlCafQMgodA9y4DrcdJZZ0RLErdH3yHlK8nVwuZvR52QCCi1M2siGSY07-C1LcYIW1nsHX_4D7OKX5QtnM91NSY0UX6s2JsinmnFxvDsmPkO4MwWZJxizJGCnNksyMvzpLp3Z03QN8jmIGNidgnwts3T0AqXg7uAcbOb-L9r5rd5CMC7Pl5cnSQzSwTT6bLz8oxgJjrjRjfwFzcquH</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Uchida, Y. 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| subjects | Animals Antibodies, Monoclonal - immunology Antigens, Protozoan CELLS Cercopithecus aethiops COWS DOGS Female HOST PARASITE RELATIONS invasion process Mice Mice, Inbred BALB C MONOCLONAL ANTIBODIES monoclonal antibody Neospora - immunology Neospora - pathogenicity NEOSPORA CANINUM surface antigen Vero Cells |
| title | Monoclonal antibodies preventing invasion of Neospora caninum tachyzoites into host cells |
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