No evidence for recombination between HIV type 1 and HIV type 2 within the envelope region in dually seropositive individuals from Senegal
To investigate the frequency of recombination between HIV-1 and HIV-2 in vivo during dual infection, we performed a retrospective analysis of blood samples from 46 dual HIV-1/HIV-2-seropositive adults for evidence of recombination. HIV viral DNA from peripheral blood mononuclear cells (PBMC) was sub...
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| Veröffentlicht in: | AIDS research and human retroviruses 2004-09, Vol.20 (9), p.958-963 |
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| container_title | AIDS research and human retroviruses |
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| creator | CURLIN, Marcel E GOTTLIEB, Geoffrey S HAWES, Stephen E SOW, Papa Salif NDOYE, Ibra CRITCHLOW, Cathy W KIVIAT, Nancy B MULLINS, James I |
| description | To investigate the frequency of recombination between HIV-1 and HIV-2 in vivo during dual infection, we performed a retrospective analysis of blood samples from 46 dual HIV-1/HIV-2-seropositive adults for evidence of recombination. HIV viral DNA from peripheral blood mononuclear cells (PBMC) was subjected to two separate nested polymerase chain reaction (PCR) assays using opposing HIV-1 and HIV-2 primer pairs selected to flank a approximately 650-base pair region including the V3 loop of the envelope gene. In the first assay, primers were chosen to amplify recombinants with HIV-1 on the 5' end and HIV-2 on the 3' end, and in the second assay, primers were chosen to amplify recombinants with the opposite orientation. All PCR experiments were run in parallel with positive controls consisting of partial-length env fragments bearing a single central HIV-1/2 recombination site, and appropriate primer-binding sites on each end. The limit of detection for both assays was |
| doi_str_mv | 10.1089/aid.2004.20.958 |
| format | Article |
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HIV viral DNA from peripheral blood mononuclear cells (PBMC) was subjected to two separate nested polymerase chain reaction (PCR) assays using opposing HIV-1 and HIV-2 primer pairs selected to flank a approximately 650-base pair region including the V3 loop of the envelope gene. In the first assay, primers were chosen to amplify recombinants with HIV-1 on the 5' end and HIV-2 on the 3' end, and in the second assay, primers were chosen to amplify recombinants with the opposite orientation. All PCR experiments were run in parallel with positive controls consisting of partial-length env fragments bearing a single central HIV-1/2 recombination site, and appropriate primer-binding sites on each end. The limit of detection for both assays was <10 copies of recombinant product per 150,000 cell equivalents of input PBMC DNA. In all 46 dually seropositive patients in this study, PCR screening of PBMC failed to detect evidence of HIV-1/HIV-2 recombinants in the C2-V5 env region. Although genetic recombination between HIV-1 and HIV-2 may occur, we conclude that any such events within env are exceedingly rare, and do not result in the outgrowth of recombinant strains.</description><identifier>ISSN: 0889-2229</identifier><identifier>EISSN: 1931-8405</identifier><identifier>DOI: 10.1089/aid.2004.20.958</identifier><identifier>PMID: 15585083</identifier><identifier>CODEN: ARHRE7</identifier><language>eng</language><publisher>Larchmont, NY: Liebert</publisher><subject>Adult ; AIDS/HIV ; Biological and medical sciences ; DNA, Viral - analysis ; DNA, Viral - blood ; Female ; Fundamental and applied biological sciences. Psychology ; Genes, env - genetics ; HIV Seropositivity - complications ; HIV Seropositivity - virology ; HIV-1 - genetics ; HIV-1 - isolation & purification ; HIV-2 - genetics ; HIV-2 - isolation & purification ; Human immunodeficiency virus 1 ; Human immunodeficiency virus 2 ; Human viral diseases ; Humans ; Infectious diseases ; Male ; Medical sciences ; Microbiology ; Middle Aged ; Miscellaneous ; Polymerase Chain Reaction ; Recombination, Genetic ; Senegal ; Viral diseases ; Viral diseases of the lymphoid tissue and the blood. Aids ; Virology</subject><ispartof>AIDS research and human retroviruses, 2004-09, Vol.20 (9), p.958-963</ispartof><rights>2004 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c354t-cb5756554f0aafa8508f15a956cdf49e88da60d66d1ee32be7c6aa391875595b3</citedby><cites>FETCH-LOGICAL-c354t-cb5756554f0aafa8508f15a956cdf49e88da60d66d1ee32be7c6aa391875595b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3042,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16193451$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15585083$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CURLIN, Marcel E</creatorcontrib><creatorcontrib>GOTTLIEB, Geoffrey S</creatorcontrib><creatorcontrib>HAWES, Stephen E</creatorcontrib><creatorcontrib>SOW, Papa Salif</creatorcontrib><creatorcontrib>NDOYE, Ibra</creatorcontrib><creatorcontrib>CRITCHLOW, Cathy W</creatorcontrib><creatorcontrib>KIVIAT, Nancy B</creatorcontrib><creatorcontrib>MULLINS, James I</creatorcontrib><title>No evidence for recombination between HIV type 1 and HIV type 2 within the envelope region in dually seropositive individuals from Senegal</title><title>AIDS research and human retroviruses</title><addtitle>AIDS Res Hum Retroviruses</addtitle><description>To investigate the frequency of recombination between HIV-1 and HIV-2 in vivo during dual infection, we performed a retrospective analysis of blood samples from 46 dual HIV-1/HIV-2-seropositive adults for evidence of recombination. HIV viral DNA from peripheral blood mononuclear cells (PBMC) was subjected to two separate nested polymerase chain reaction (PCR) assays using opposing HIV-1 and HIV-2 primer pairs selected to flank a approximately 650-base pair region including the V3 loop of the envelope gene. In the first assay, primers were chosen to amplify recombinants with HIV-1 on the 5' end and HIV-2 on the 3' end, and in the second assay, primers were chosen to amplify recombinants with the opposite orientation. All PCR experiments were run in parallel with positive controls consisting of partial-length env fragments bearing a single central HIV-1/2 recombination site, and appropriate primer-binding sites on each end. The limit of detection for both assays was <10 copies of recombinant product per 150,000 cell equivalents of input PBMC DNA. In all 46 dually seropositive patients in this study, PCR screening of PBMC failed to detect evidence of HIV-1/HIV-2 recombinants in the C2-V5 env region. Although genetic recombination between HIV-1 and HIV-2 may occur, we conclude that any such events within env are exceedingly rare, and do not result in the outgrowth of recombinant strains.</description><subject>Adult</subject><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - blood</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes, env - genetics</subject><subject>HIV Seropositivity - complications</subject><subject>HIV Seropositivity - virology</subject><subject>HIV-1 - genetics</subject><subject>HIV-1 - isolation & purification</subject><subject>HIV-2 - genetics</subject><subject>HIV-2 - isolation & purification</subject><subject>Human immunodeficiency virus 1</subject><subject>Human immunodeficiency virus 2</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Middle Aged</subject><subject>Miscellaneous</subject><subject>Polymerase Chain Reaction</subject><subject>Recombination, Genetic</subject><subject>Senegal</subject><subject>Viral diseases</subject><subject>Viral diseases of the lymphoid tissue and the blood. Aids</subject><subject>Virology</subject><issn>0889-2229</issn><issn>1931-8405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9vFCEUx4nR2LV69ma42Nts-TFvBo6mUduk0YOt1wkDjxYzAyvMbrP_gn912XSTPcrhEb583iPkQ8hHztacKX1pglsLxtpa1hrUK7LiWvJGtQxekxVTSjdCCH1G3pXyhzGmhYC35IwDKGBKrsi_H4niLjiMFqlPmWa0aR5DNEtIkY64PCFGen3zmy77DVJOTXSno6BPYXkMkS6PSDHucEo1zfhwaK6x25pp2tOCOW1SCUvYYY1dqC_Wm0J9TjP9hREfzPSevPE1ww_H_Zzcf_t6d3Xd3P78fnP15baxEtqlsSP00AG0nhnjzeEfnoPR0FnnW41KOdMx13WOI0oxYm87Y6TmqgfQMMpzcvEyd5PT3y2WZZhDsThNJmLalqHreSdkXf8Ded9L6KWu4OULaHMqJaMfNjnMJu8HzoaDp6F6Gg6eahmqp9rx6Th6O87oTvxRTAU-HwFTrJl8NtGGcuK66rkFLp8BE1Ccxg</recordid><startdate>20040901</startdate><enddate>20040901</enddate><creator>CURLIN, Marcel E</creator><creator>GOTTLIEB, Geoffrey S</creator><creator>HAWES, Stephen E</creator><creator>SOW, Papa Salif</creator><creator>NDOYE, Ibra</creator><creator>CRITCHLOW, Cathy W</creator><creator>KIVIAT, Nancy B</creator><creator>MULLINS, James I</creator><general>Liebert</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20040901</creationdate><title>No evidence for recombination between HIV type 1 and HIV type 2 within the envelope region in dually seropositive individuals from Senegal</title><author>CURLIN, Marcel E ; GOTTLIEB, Geoffrey S ; HAWES, Stephen E ; SOW, Papa Salif ; NDOYE, Ibra ; CRITCHLOW, Cathy W ; KIVIAT, Nancy B ; MULLINS, James I</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c354t-cb5756554f0aafa8508f15a956cdf49e88da60d66d1ee32be7c6aa391875595b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adult</topic><topic>AIDS/HIV</topic><topic>Biological and medical sciences</topic><topic>DNA, Viral - analysis</topic><topic>DNA, Viral - blood</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes, env - genetics</topic><topic>HIV Seropositivity - complications</topic><topic>HIV Seropositivity - virology</topic><topic>HIV-1 - genetics</topic><topic>HIV-1 - isolation & purification</topic><topic>HIV-2 - genetics</topic><topic>HIV-2 - isolation & purification</topic><topic>Human immunodeficiency virus 1</topic><topic>Human immunodeficiency virus 2</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Middle Aged</topic><topic>Miscellaneous</topic><topic>Polymerase Chain Reaction</topic><topic>Recombination, Genetic</topic><topic>Senegal</topic><topic>Viral diseases</topic><topic>Viral diseases of the lymphoid tissue and the blood. Aids</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CURLIN, Marcel E</creatorcontrib><creatorcontrib>GOTTLIEB, Geoffrey S</creatorcontrib><creatorcontrib>HAWES, Stephen E</creatorcontrib><creatorcontrib>SOW, Papa Salif</creatorcontrib><creatorcontrib>NDOYE, Ibra</creatorcontrib><creatorcontrib>CRITCHLOW, Cathy W</creatorcontrib><creatorcontrib>KIVIAT, Nancy B</creatorcontrib><creatorcontrib>MULLINS, James I</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>AIDS research and human retroviruses</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>CURLIN, Marcel E</au><au>GOTTLIEB, Geoffrey S</au><au>HAWES, Stephen E</au><au>SOW, Papa Salif</au><au>NDOYE, Ibra</au><au>CRITCHLOW, Cathy W</au><au>KIVIAT, Nancy B</au><au>MULLINS, James I</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>No evidence for recombination between HIV type 1 and HIV type 2 within the envelope region in dually seropositive individuals from Senegal</atitle><jtitle>AIDS research and human retroviruses</jtitle><addtitle>AIDS Res Hum Retroviruses</addtitle><date>2004-09-01</date><risdate>2004</risdate><volume>20</volume><issue>9</issue><spage>958</spage><epage>963</epage><pages>958-963</pages><issn>0889-2229</issn><eissn>1931-8405</eissn><coden>ARHRE7</coden><abstract>To investigate the frequency of recombination between HIV-1 and HIV-2 in vivo during dual infection, we performed a retrospective analysis of blood samples from 46 dual HIV-1/HIV-2-seropositive adults for evidence of recombination. HIV viral DNA from peripheral blood mononuclear cells (PBMC) was subjected to two separate nested polymerase chain reaction (PCR) assays using opposing HIV-1 and HIV-2 primer pairs selected to flank a approximately 650-base pair region including the V3 loop of the envelope gene. In the first assay, primers were chosen to amplify recombinants with HIV-1 on the 5' end and HIV-2 on the 3' end, and in the second assay, primers were chosen to amplify recombinants with the opposite orientation. All PCR experiments were run in parallel with positive controls consisting of partial-length env fragments bearing a single central HIV-1/2 recombination site, and appropriate primer-binding sites on each end. The limit of detection for both assays was <10 copies of recombinant product per 150,000 cell equivalents of input PBMC DNA. In all 46 dually seropositive patients in this study, PCR screening of PBMC failed to detect evidence of HIV-1/HIV-2 recombinants in the C2-V5 env region. Although genetic recombination between HIV-1 and HIV-2 may occur, we conclude that any such events within env are exceedingly rare, and do not result in the outgrowth of recombinant strains.</abstract><cop>Larchmont, NY</cop><pub>Liebert</pub><pmid>15585083</pmid><doi>10.1089/aid.2004.20.958</doi><tpages>6</tpages></addata></record> |
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| ispartof | AIDS research and human retroviruses, 2004-09, Vol.20 (9), p.958-963 |
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| source | Mary Ann Liebert Online Subscription; MEDLINE; Alma/SFX Local Collection |
| subjects | Adult AIDS/HIV Biological and medical sciences DNA, Viral - analysis DNA, Viral - blood Female Fundamental and applied biological sciences. Psychology Genes, env - genetics HIV Seropositivity - complications HIV Seropositivity - virology HIV-1 - genetics HIV-1 - isolation & purification HIV-2 - genetics HIV-2 - isolation & purification Human immunodeficiency virus 1 Human immunodeficiency virus 2 Human viral diseases Humans Infectious diseases Male Medical sciences Microbiology Middle Aged Miscellaneous Polymerase Chain Reaction Recombination, Genetic Senegal Viral diseases Viral diseases of the lymphoid tissue and the blood. Aids Virology |
| title | No evidence for recombination between HIV type 1 and HIV type 2 within the envelope region in dually seropositive individuals from Senegal |
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