Redox Buffering of Hypochlorous Acid by Thiocyanate in Physiologic Fluids
The major antimicrobial products of neutrophilic myeloperoxidase (MPO) in physiologic fluids are hypochlorous acid (HOCl) and hypothiocyanite (OSCN-), and the former is generally believed to be the killing agent. However, we have determined that HOCl oxidizes SCN- in a facile nonenzymic reaction. Th...
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| Veröffentlicht in: | Journal of the American Chemical Society 2004-12, Vol.126 (49), p.15976-15977 |
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| container_end_page | 15977 |
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| container_issue | 49 |
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| container_title | Journal of the American Chemical Society |
| container_volume | 126 |
| creator | Ashby, Michael T Carlson, Amy C Scott, M. Jared |
| description | The major antimicrobial products of neutrophilic myeloperoxidase (MPO) in physiologic fluids are hypochlorous acid (HOCl) and hypothiocyanite (OSCN-), and the former is generally believed to be the killing agent. However, we have determined that HOCl oxidizes SCN- in a facile nonenzymic reaction. The observed kinetics and computational models substantiate the hypothesis that SCN- serves to moderate the potential autotoxicity of HOCl by restricting its lifetime in physiologic fluids. Furthermore, the oxidizing equivalents of HOCl are preserved in OSCN-, a more discriminate biocide that is not lethal to mammalian cells. |
| doi_str_mv | 10.1021/ja0438361 |
| format | Article |
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Furthermore, the oxidizing equivalents of HOCl are preserved in OSCN-, a more discriminate biocide that is not lethal to mammalian cells.</description><subject>Biological and medical sciences</subject><subject>Buffers</subject><subject>Electrochemistry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydrogen Peroxide - chemistry</subject><subject>Hydrogen Peroxide - metabolism</subject><subject>Hypochlorous Acid - blood</subject><subject>Hypochlorous Acid - chemistry</subject><subject>Hypochlorous Acid - metabolism</subject><subject>Kinetics</subject><subject>Molecular biophysics</subject><subject>Oxidation-Reduction</subject><subject>Peroxidase - metabolism</subject><subject>Physical chemistry in biology</subject><subject>Serum Albumin - chemistry</subject><subject>Serum Albumin - metabolism</subject><subject>Substrate Specificity</subject><subject>Thiocyanates - blood</subject><subject>Thiocyanates - chemistry</subject><subject>Thiocyanates - metabolism</subject><issn>0002-7863</issn><issn>1520-5126</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNptkEtrGzEURkVoSJzHIn-gaNNCFpPqLc0ydfOClIbEgeyERnMnljseuZIHMv8-E2ziTVfiosPh4yB0RskFJYz-WDgiuOGK7qEJlYwUkjL1BU0IIazQRvFDdJTzYjwFM_QAHVIpjdBMT9DdI9TxDf_smwZS6F5xbPDtsIp-3sYU-4wvfahxNeDZPEQ_uM6tAYcOP8yHHGIbX4PH120f6nyC9hvXZjjdvsfo-fpqNr0t7v_c3E0v7wsnhF4XpTSaKKdLBQQ8-JKyRgjQtapqKSovGADXpRESKkMrkIJJpbn3Vem1IZwfo-8b7yrFfz3ktV2G7KFtXQfjYKs0VcSUZATPN6BPMecEjV2lsHRpsJTYj272s9vIft1K-2oJ9Y7chhqBb1vAZe_aJrnOh7zjFBeakw9RseFCXsPb579Lf8dhXEs7e3iyL2p68-vxN7d853U-20XsUze2-8_AdwBCjpw</recordid><startdate>20041215</startdate><enddate>20041215</enddate><creator>Ashby, Michael T</creator><creator>Carlson, Amy C</creator><creator>Scott, M. 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Psychology</topic><topic>Humans</topic><topic>Hydrogen Peroxide - chemistry</topic><topic>Hydrogen Peroxide - metabolism</topic><topic>Hypochlorous Acid - blood</topic><topic>Hypochlorous Acid - chemistry</topic><topic>Hypochlorous Acid - metabolism</topic><topic>Kinetics</topic><topic>Molecular biophysics</topic><topic>Oxidation-Reduction</topic><topic>Peroxidase - metabolism</topic><topic>Physical chemistry in biology</topic><topic>Serum Albumin - chemistry</topic><topic>Serum Albumin - metabolism</topic><topic>Substrate Specificity</topic><topic>Thiocyanates - blood</topic><topic>Thiocyanates - chemistry</topic><topic>Thiocyanates - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ashby, Michael T</creatorcontrib><creatorcontrib>Carlson, Amy C</creatorcontrib><creatorcontrib>Scott, M. Jared</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of the American Chemical Society</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ashby, Michael T</au><au>Carlson, Amy C</au><au>Scott, M. Jared</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Redox Buffering of Hypochlorous Acid by Thiocyanate in Physiologic Fluids</atitle><jtitle>Journal of the American Chemical Society</jtitle><addtitle>J. Am. Chem. Soc</addtitle><date>2004-12-15</date><risdate>2004</risdate><volume>126</volume><issue>49</issue><spage>15976</spage><epage>15977</epage><pages>15976-15977</pages><issn>0002-7863</issn><eissn>1520-5126</eissn><coden>JACSAT</coden><abstract>The major antimicrobial products of neutrophilic myeloperoxidase (MPO) in physiologic fluids are hypochlorous acid (HOCl) and hypothiocyanite (OSCN-), and the former is generally believed to be the killing agent. However, we have determined that HOCl oxidizes SCN- in a facile nonenzymic reaction. The observed kinetics and computational models substantiate the hypothesis that SCN- serves to moderate the potential autotoxicity of HOCl by restricting its lifetime in physiologic fluids. Furthermore, the oxidizing equivalents of HOCl are preserved in OSCN-, a more discriminate biocide that is not lethal to mammalian cells.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>15584727</pmid><doi>10.1021/ja0438361</doi><tpages>2</tpages></addata></record> |
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| language | eng |
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| subjects | Biological and medical sciences Buffers Electrochemistry Fundamental and applied biological sciences. Psychology Humans Hydrogen Peroxide - chemistry Hydrogen Peroxide - metabolism Hypochlorous Acid - blood Hypochlorous Acid - chemistry Hypochlorous Acid - metabolism Kinetics Molecular biophysics Oxidation-Reduction Peroxidase - metabolism Physical chemistry in biology Serum Albumin - chemistry Serum Albumin - metabolism Substrate Specificity Thiocyanates - blood Thiocyanates - chemistry Thiocyanates - metabolism |
| title | Redox Buffering of Hypochlorous Acid by Thiocyanate in Physiologic Fluids |
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