Development of a loop-mediated isothermal amplification assay for sensitive and rapid detection of Campylobacter fetus

We developed a sensitive and rapid loop-mediated isothermal amplification (LAMP) assay for detection of Campylobacter fetus. This assay provides simpler and more rapid detection of C. fetus than conventional biochemical and PCR assays. The assay correctly detected 60 C. fetus strains but not 55 non-...

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Veröffentlicht in:	Veterinary microbiology 2009-05, Vol.136 (3), p.393-396
Hauptverfasser:	Yamazaki, Wataru, Taguchi, Masumi, Ishibashi, Masanori, Nukina, Masafumi, Misawa, Naoaki, Inoue, Kiyoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	accuracy animal tissues Animals Bacteriology bioassays Biological and medical sciences Campylobacter fetus Campylobacter fetus - genetics Campylobacter fetus - isolation & purification Campylobacter Infections - diagnosis Campylobacter Infections - microbiology campylobacteriosis Cattle cattle diseases Cattle Diseases - diagnosis Cattle Diseases - microbiology disease detection disease diagnosis DNA - chemistry DNA - genetics Female food pathogens Fundamental and applied biological sciences. Psychology human diseases Liver - microbiology loop-mediated isothermal amplification Loop-mediated isothermal amplification (LAMP) assay Microbiology Miscellaneous molecular epidemiology new methods Nucleic Acid Amplification Techniques - methods Nucleic Acid Amplification Techniques - veterinary nucleic acids pathogen identification polymerase chain reaction rapid methods Sensitive and rapid detection Sensitivity and Specificity Vagina - microbiology validity
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