TGF-beta1, -beta2 and -beta3 cooperate to facilitate tubulogenesis in the explanted quail heart
Transforming growth factor-beta (TGF-beta) isoforms have been implicated as both pro- and anti-angiogenic modulators. In this study we addressed the roles of TGF-beta isoforms on coronary tubulogenesis. Embryonic (E6) quail ventricular specimens were explanted onto collagen gels allowing endothelial...
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| Veröffentlicht in: | Journal of vascular research 2004-11, Vol.41 (6), p.491-498 |
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| creator | Holifield, Jennifer S Arlen, Angela M Runyan, Raymond B Tomanek, Robert J |
| description | Transforming growth factor-beta (TGF-beta) isoforms have been implicated as both pro- and anti-angiogenic modulators. In this study we addressed the roles of TGF-beta isoforms on coronary tubulogenesis.
Embryonic (E6) quail ventricular specimens were explanted onto collagen gels allowing endothelial cells to migrate and form vascular tubes. Growth factors and/or neutralizing growth factor antibodies were added to the cultures. Endothelial cells were identified using a quail endothelial cell marker, QH1. Image analysis was used to quantify aggregate tube length.
Addition of any isoform (TGF-beta(1), TGF-beta(2) or TGF-beta(3)) virtually prevented tubulogenesis (>95% inhibition), while stimulation of tubulogenesis occurred by adding neutralizing antibodies to TGF-beta(3), but not to TGF-beta(1) or -beta(2). When all three isoforms were added, tubulogenesis was enhanced, indicating the key role of TGF-beta(3). Documentation of the inhibitory effect of TGF-beta isoforms on tubulogenesis is further supported by our experiments in which the marked enhancement of tube formation by bFGF and VEGF was negated when exogenous TGF-beta(1), -beta(2), or -beta(3) were added to the cultures.
(1) TGF-beta(1), -beta(2) and -beta(3) each inhibits angiogenesis; (2) cooperation between the three TGF-beta isoforms and other angiogenic factors is essential for the regulation of normal tubulogenesis and (3) the stimulatory effect of VEGF or bFGF on tubulogenesis is negated by exogenous TGB-betas. |
| format | Article |
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Embryonic (E6) quail ventricular specimens were explanted onto collagen gels allowing endothelial cells to migrate and form vascular tubes. Growth factors and/or neutralizing growth factor antibodies were added to the cultures. Endothelial cells were identified using a quail endothelial cell marker, QH1. Image analysis was used to quantify aggregate tube length.
Addition of any isoform (TGF-beta(1), TGF-beta(2) or TGF-beta(3)) virtually prevented tubulogenesis (>95% inhibition), while stimulation of tubulogenesis occurred by adding neutralizing antibodies to TGF-beta(3), but not to TGF-beta(1) or -beta(2). When all three isoforms were added, tubulogenesis was enhanced, indicating the key role of TGF-beta(3). Documentation of the inhibitory effect of TGF-beta isoforms on tubulogenesis is further supported by our experiments in which the marked enhancement of tube formation by bFGF and VEGF was negated when exogenous TGF-beta(1), -beta(2), or -beta(3) were added to the cultures.
(1) TGF-beta(1), -beta(2) and -beta(3) each inhibits angiogenesis; (2) cooperation between the three TGF-beta isoforms and other angiogenic factors is essential for the regulation of normal tubulogenesis and (3) the stimulatory effect of VEGF or bFGF on tubulogenesis is negated by exogenous TGB-betas.</description><identifier>ISSN: 1018-1172</identifier><identifier>PMID: 15528931</identifier><language>eng</language><publisher>Switzerland</publisher><subject>Animals ; Antibodies - pharmacology ; Coturnix ; Fibroblast Growth Factor 2 - pharmacology ; Heart - drug effects ; Heart - embryology ; Heart - physiology ; Neovascularization, Physiologic - drug effects ; Organ Culture Techniques ; Transforming Growth Factor beta - immunology ; Transforming Growth Factor beta - pharmacology ; Transforming Growth Factor beta1 ; Transforming Growth Factor beta2 ; Transforming Growth Factor beta3 ; Vascular Endothelial Growth Factor A - pharmacology</subject><ispartof>Journal of vascular research, 2004-11, Vol.41 (6), p.491-498</ispartof><rights>Copyright 2004 S. Karger AG, Basel.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15528931$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Holifield, Jennifer S</creatorcontrib><creatorcontrib>Arlen, Angela M</creatorcontrib><creatorcontrib>Runyan, Raymond B</creatorcontrib><creatorcontrib>Tomanek, Robert J</creatorcontrib><title>TGF-beta1, -beta2 and -beta3 cooperate to facilitate tubulogenesis in the explanted quail heart</title><title>Journal of vascular research</title><addtitle>J Vasc Res</addtitle><description>Transforming growth factor-beta (TGF-beta) isoforms have been implicated as both pro- and anti-angiogenic modulators. In this study we addressed the roles of TGF-beta isoforms on coronary tubulogenesis.
Embryonic (E6) quail ventricular specimens were explanted onto collagen gels allowing endothelial cells to migrate and form vascular tubes. Growth factors and/or neutralizing growth factor antibodies were added to the cultures. Endothelial cells were identified using a quail endothelial cell marker, QH1. Image analysis was used to quantify aggregate tube length.
Addition of any isoform (TGF-beta(1), TGF-beta(2) or TGF-beta(3)) virtually prevented tubulogenesis (>95% inhibition), while stimulation of tubulogenesis occurred by adding neutralizing antibodies to TGF-beta(3), but not to TGF-beta(1) or -beta(2). When all three isoforms were added, tubulogenesis was enhanced, indicating the key role of TGF-beta(3). Documentation of the inhibitory effect of TGF-beta isoforms on tubulogenesis is further supported by our experiments in which the marked enhancement of tube formation by bFGF and VEGF was negated when exogenous TGF-beta(1), -beta(2), or -beta(3) were added to the cultures.
(1) TGF-beta(1), -beta(2) and -beta(3) each inhibits angiogenesis; (2) cooperation between the three TGF-beta isoforms and other angiogenic factors is essential for the regulation of normal tubulogenesis and (3) the stimulatory effect of VEGF or bFGF on tubulogenesis is negated by exogenous TGB-betas.</description><subject>Animals</subject><subject>Antibodies - pharmacology</subject><subject>Coturnix</subject><subject>Fibroblast Growth Factor 2 - pharmacology</subject><subject>Heart - drug effects</subject><subject>Heart - embryology</subject><subject>Heart - physiology</subject><subject>Neovascularization, Physiologic - drug effects</subject><subject>Organ Culture Techniques</subject><subject>Transforming Growth Factor beta - immunology</subject><subject>Transforming Growth Factor beta - pharmacology</subject><subject>Transforming Growth Factor beta1</subject><subject>Transforming Growth Factor beta2</subject><subject>Transforming Growth Factor beta3</subject><subject>Vascular Endothelial Growth Factor A - pharmacology</subject><issn>1018-1172</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMFLwzAYxXNQ3Jz-C5KTJwv9kjRtjjLcFAZeei9J-8VF2qZrEtD_3rLN03sPfjwe74asIYcqAyjZityH8J3nIFQl78gKioJVisOaNPV-lxmMGl7oWRnVY3exnLbeTzjriDR6anXrehfPKZnU-y8cMbhA3UjjESn-TL0eI3b0lLTr6RH1HB_IrdV9wMerbki9e6u379nhc_-xfT1kUyEgs8iNlWAlKrM404EyUiIAKs6kwbwSrEQmFDOgjbYyN4ILRKM6xlou-YY8X2qn2Z8ShtgMLrTYL4PQp9DIEgpZSbaAT1cwmQG7ZprdoOff5v8R_gdfclvk</recordid><startdate>200411</startdate><enddate>200411</enddate><creator>Holifield, Jennifer S</creator><creator>Arlen, Angela M</creator><creator>Runyan, Raymond B</creator><creator>Tomanek, Robert J</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200411</creationdate><title>TGF-beta1, -beta2 and -beta3 cooperate to facilitate tubulogenesis in the explanted quail heart</title><author>Holifield, Jennifer S ; Arlen, Angela M ; Runyan, Raymond B ; Tomanek, Robert J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p541-fe3bf61f6e9b3bfbd19b66e11e9326be08427e2492b1abaf60b434eeb9d22c363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Antibodies - pharmacology</topic><topic>Coturnix</topic><topic>Fibroblast Growth Factor 2 - pharmacology</topic><topic>Heart - drug effects</topic><topic>Heart - embryology</topic><topic>Heart - physiology</topic><topic>Neovascularization, Physiologic - drug effects</topic><topic>Organ Culture Techniques</topic><topic>Transforming Growth Factor beta - immunology</topic><topic>Transforming Growth Factor beta - pharmacology</topic><topic>Transforming Growth Factor beta1</topic><topic>Transforming Growth Factor beta2</topic><topic>Transforming Growth Factor beta3</topic><topic>Vascular Endothelial Growth Factor A - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Holifield, Jennifer S</creatorcontrib><creatorcontrib>Arlen, Angela M</creatorcontrib><creatorcontrib>Runyan, Raymond B</creatorcontrib><creatorcontrib>Tomanek, Robert J</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of vascular research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Holifield, Jennifer S</au><au>Arlen, Angela M</au><au>Runyan, Raymond B</au><au>Tomanek, Robert J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>TGF-beta1, -beta2 and -beta3 cooperate to facilitate tubulogenesis in the explanted quail heart</atitle><jtitle>Journal of vascular research</jtitle><addtitle>J Vasc Res</addtitle><date>2004-11</date><risdate>2004</risdate><volume>41</volume><issue>6</issue><spage>491</spage><epage>498</epage><pages>491-498</pages><issn>1018-1172</issn><abstract>Transforming growth factor-beta (TGF-beta) isoforms have been implicated as both pro- and anti-angiogenic modulators. In this study we addressed the roles of TGF-beta isoforms on coronary tubulogenesis.
Embryonic (E6) quail ventricular specimens were explanted onto collagen gels allowing endothelial cells to migrate and form vascular tubes. Growth factors and/or neutralizing growth factor antibodies were added to the cultures. Endothelial cells were identified using a quail endothelial cell marker, QH1. Image analysis was used to quantify aggregate tube length.
Addition of any isoform (TGF-beta(1), TGF-beta(2) or TGF-beta(3)) virtually prevented tubulogenesis (>95% inhibition), while stimulation of tubulogenesis occurred by adding neutralizing antibodies to TGF-beta(3), but not to TGF-beta(1) or -beta(2). When all three isoforms were added, tubulogenesis was enhanced, indicating the key role of TGF-beta(3). Documentation of the inhibitory effect of TGF-beta isoforms on tubulogenesis is further supported by our experiments in which the marked enhancement of tube formation by bFGF and VEGF was negated when exogenous TGF-beta(1), -beta(2), or -beta(3) were added to the cultures.
(1) TGF-beta(1), -beta(2) and -beta(3) each inhibits angiogenesis; (2) cooperation between the three TGF-beta isoforms and other angiogenic factors is essential for the regulation of normal tubulogenesis and (3) the stimulatory effect of VEGF or bFGF on tubulogenesis is negated by exogenous TGB-betas.</abstract><cop>Switzerland</cop><pmid>15528931</pmid><tpages>8</tpages></addata></record> |
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| ispartof | Journal of vascular research, 2004-11, Vol.41 (6), p.491-498 |
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| source | Karger e-journals Complete Collection; MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Antibodies - pharmacology Coturnix Fibroblast Growth Factor 2 - pharmacology Heart - drug effects Heart - embryology Heart - physiology Neovascularization, Physiologic - drug effects Organ Culture Techniques Transforming Growth Factor beta - immunology Transforming Growth Factor beta - pharmacology Transforming Growth Factor beta1 Transforming Growth Factor beta2 Transforming Growth Factor beta3 Vascular Endothelial Growth Factor A - pharmacology |
| title | TGF-beta1, -beta2 and -beta3 cooperate to facilitate tubulogenesis in the explanted quail heart |
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