Generating and characterizing monoclonal and polyclonal antibodies against avian H5N1 hemagglutinin protein

Accurate and timely diagnoses are central to H5N1 infection control. Here we describe the cloning and expression of the HA1 protein of the A/Vietnam/1203/04 strain in a bacterial system to generate mono-/polyclonal antibodies. All of the eight generated monoclonal antibodies recognized the same line...

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Veröffentlicht in:	Biochemical and biophysical research communications 2009-05, Vol.382 (4), p.691-696
Hauptverfasser:	Wang, Sheng-Fan, Chen, Kuan-Hsuan, Thitithanyanont, Arunee, Yao, Ling, Lee, Yuan-Ming, Chan, Yu-Jiun, Liu, Shih-Jen, Chong, Pele, Liu, Wu-Tse, Huang, Jason C., Chen, Yi-Ming Arthur
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - biosynthesis Antibodies, Monoclonal - immunology Antibodies, Viral - biosynthesis Antibodies, Viral - immunology Birds Cell Line Clade Cloning, Molecular Dogs Epitope Epitope Mapping H5N1 virus Hemagglutinin Hemagglutinin Glycoproteins, Influenza Virus - biosynthesis Hemagglutinin Glycoproteins, Influenza Virus - immunology Humans Hybridomas Influenza A Virus, H5N1 Subtype - immunology Influenza in Birds - immunology Influenza in Birds - prevention & control Monoclonal antibodies Polyclonal antibodies
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