Sphingosine-1-phosphate regulates RGS2 and RGS16 mRNA expression in vascular smooth muscle cells
Regulator of G protein signalling (RGS) protein expression is altered under growth promoting conditions in vascular smooth muscle cells (VSMCs). Since sphingosine-1-phosphate (S1P) is an important growth stimulatory factor, we investigated whether stimulation of VSMCs with S1P results in alterations...
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| Veröffentlicht in: | European journal of pharmacology 2009-03, Vol.606 (1), p.25-31 |
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| container_title | European journal of pharmacology |
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| creator | Hendriks-Balk, Mariëlle C. Hajji, Najat van Loenen, Pieter B. Michel, Martin C. Peters, Stephan L.M. Alewijnse, Astrid E. |
| description | Regulator of G protein signalling (RGS) protein expression is altered under growth promoting conditions in vascular smooth muscle cells (VSMCs). Since sphingosine-1-phosphate (S1P) is an important growth stimulatory factor, we investigated whether stimulation of VSMCs with S1P results in alterations in mRNA expression levels of several RGS proteins and which signalling components are involved.
VSMCs were stimulated with S1P and mRNA expression levels of RGS2, RGS3, RGS4, RGS5 and RGS16 were measured by real-time polymerase chain reaction. S1P caused a time-dependent up-regulation of RGS2 and RGS16 mRNA expression. FTY720-P, a S1P
1/S1P
3–5 agonist, did not regulate RGS2 mRNA levels although it did up-regulate RGS16 mRNA expression. Pertussis toxin treatment revealed that the S1P-induced RGS16 expression was G
i/o-dependent whereas up-regulation of RGS2 mRNA was not. Phosphatidylinositol 3-kinase, protein kinase C and mitogen-activated protein kinase kinase apparently were not involved in the S1P-induced up-regulation of both RGS proteins.
The present study demonstrates that S1P induces RGS2 and RGS16 mRNA expression but uses distinct S1P receptor subtypes and signalling pathways to regulate expression of these RGS proteins. |
| doi_str_mv | 10.1016/j.ejphar.2009.01.018 |
| format | Article |
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VSMCs were stimulated with S1P and mRNA expression levels of RGS2, RGS3, RGS4, RGS5 and RGS16 were measured by real-time polymerase chain reaction. S1P caused a time-dependent up-regulation of RGS2 and RGS16 mRNA expression. FTY720-P, a S1P
1/S1P
3–5 agonist, did not regulate RGS2 mRNA levels although it did up-regulate RGS16 mRNA expression. Pertussis toxin treatment revealed that the S1P-induced RGS16 expression was G
i/o-dependent whereas up-regulation of RGS2 mRNA was not. Phosphatidylinositol 3-kinase, protein kinase C and mitogen-activated protein kinase kinase apparently were not involved in the S1P-induced up-regulation of both RGS proteins.
The present study demonstrates that S1P induces RGS2 and RGS16 mRNA expression but uses distinct S1P receptor subtypes and signalling pathways to regulate expression of these RGS proteins.</description><identifier>ISSN: 0014-2999</identifier><identifier>EISSN: 1879-0712</identifier><identifier>DOI: 10.1016/j.ejphar.2009.01.018</identifier><identifier>PMID: 19374869</identifier><identifier>CODEN: EJPHAZ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; FTY720-P ; Gene Expression Regulation - drug effects ; Lysophospholipids - metabolism ; Lysophospholipids - pharmacology ; Male ; Medical sciences ; Muscle, Smooth, Vascular - cytology ; Muscle, Smooth, Vascular - drug effects ; Muscle, Smooth, Vascular - metabolism ; Pharmacology. Drug treatments ; Rats ; Rats, Wistar ; Receptors, Lysosphingolipid - metabolism ; Regulator of G protein signalling ; RGS Proteins - genetics ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Signal Transduction - drug effects ; Sphingosine - analogs & derivatives ; Sphingosine - metabolism ; Sphingosine - pharmacology ; Sphingosine-1-phosphate receptor ; Up-Regulation - drug effects ; VSMC</subject><ispartof>European journal of pharmacology, 2009-03, Vol.606 (1), p.25-31</ispartof><rights>2008 Elsevier B.V.</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c390t-378ec9c7c02899c09d2d31e340c9a601f42c54ea6381a57444f5c0a9b5785c713</citedby><cites>FETCH-LOGICAL-c390t-378ec9c7c02899c09d2d31e340c9a601f42c54ea6381a57444f5c0a9b5785c713</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ejphar.2009.01.018$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21295190$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19374869$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hendriks-Balk, Mariëlle C.</creatorcontrib><creatorcontrib>Hajji, Najat</creatorcontrib><creatorcontrib>van Loenen, Pieter B.</creatorcontrib><creatorcontrib>Michel, Martin C.</creatorcontrib><creatorcontrib>Peters, Stephan L.M.</creatorcontrib><creatorcontrib>Alewijnse, Astrid E.</creatorcontrib><title>Sphingosine-1-phosphate regulates RGS2 and RGS16 mRNA expression in vascular smooth muscle cells</title><title>European journal of pharmacology</title><addtitle>Eur J Pharmacol</addtitle><description>Regulator of G protein signalling (RGS) protein expression is altered under growth promoting conditions in vascular smooth muscle cells (VSMCs). Since sphingosine-1-phosphate (S1P) is an important growth stimulatory factor, we investigated whether stimulation of VSMCs with S1P results in alterations in mRNA expression levels of several RGS proteins and which signalling components are involved.
VSMCs were stimulated with S1P and mRNA expression levels of RGS2, RGS3, RGS4, RGS5 and RGS16 were measured by real-time polymerase chain reaction. S1P caused a time-dependent up-regulation of RGS2 and RGS16 mRNA expression. FTY720-P, a S1P
1/S1P
3–5 agonist, did not regulate RGS2 mRNA levels although it did up-regulate RGS16 mRNA expression. Pertussis toxin treatment revealed that the S1P-induced RGS16 expression was G
i/o-dependent whereas up-regulation of RGS2 mRNA was not. Phosphatidylinositol 3-kinase, protein kinase C and mitogen-activated protein kinase kinase apparently were not involved in the S1P-induced up-regulation of both RGS proteins.
The present study demonstrates that S1P induces RGS2 and RGS16 mRNA expression but uses distinct S1P receptor subtypes and signalling pathways to regulate expression of these RGS proteins.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>FTY720-P</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Lysophospholipids - metabolism</subject><subject>Lysophospholipids - pharmacology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Muscle, Smooth, Vascular - cytology</subject><subject>Muscle, Smooth, Vascular - drug effects</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Pharmacology. Drug treatments</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Receptors, Lysosphingolipid - metabolism</subject><subject>Regulator of G protein signalling</subject><subject>RGS Proteins - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Signal Transduction - drug effects</subject><subject>Sphingosine - analogs & derivatives</subject><subject>Sphingosine - metabolism</subject><subject>Sphingosine - pharmacology</subject><subject>Sphingosine-1-phosphate receptor</subject><subject>Up-Regulation - drug effects</subject><subject>VSMC</subject><issn>0014-2999</issn><issn>1879-0712</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1rFEEQhhsxmDX6D0T6ordZq3o--yKEEKMQEkj03HZ6arK9zEyPXTMh_nt72EVvQkHV4am3ikeIdwhbBKw-7be0n3Y2bhWA3gKmal6IDTa1zqBG9VJsALDIlNb6VLxm3gNAqVX5SpyizuuiqfRG_Lyfdn58DOxHyjCbdoFT6Ewy0uPSp4Hl3dW9knZs1wErOdzdnEt6niIx-zBKP8onyy7BUfIQwryTw8KuJ-mo7_mNOOlsz_T22M_Ejy-X3y--Zte3V98uzq8zl2uYs7xuyGlXO1CN1g50q9ocKS_AaVsBdoVyZUG2yhu0ZV0URVc6sPqhrJvS1ZifiY-H3CmGXwvxbAbP6wd2pLCwqWosoaoggcUBdDEwR-rMFP1g42-DYFazZm8OZs1q1gCmatLa-2P-8jBQ-2_pqDIBH45AsmH7LtrRef7LKVS6RL3e_3zgKNl48hQNO0-jo9ZHcrNpg___J38A0SuXqA</recordid><startdate>20090315</startdate><enddate>20090315</enddate><creator>Hendriks-Balk, Mariëlle C.</creator><creator>Hajji, Najat</creator><creator>van Loenen, Pieter B.</creator><creator>Michel, Martin C.</creator><creator>Peters, Stephan L.M.</creator><creator>Alewijnse, Astrid E.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090315</creationdate><title>Sphingosine-1-phosphate regulates RGS2 and RGS16 mRNA expression in vascular smooth muscle cells</title><author>Hendriks-Balk, Mariëlle C. ; Hajji, Najat ; van Loenen, Pieter B. ; Michel, Martin C. ; Peters, Stephan L.M. ; Alewijnse, Astrid E.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c390t-378ec9c7c02899c09d2d31e340c9a601f42c54ea6381a57444f5c0a9b5785c713</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>FTY720-P</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Lysophospholipids - metabolism</topic><topic>Lysophospholipids - pharmacology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Muscle, Smooth, Vascular - cytology</topic><topic>Muscle, Smooth, Vascular - drug effects</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Pharmacology. Drug treatments</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Receptors, Lysosphingolipid - metabolism</topic><topic>Regulator of G protein signalling</topic><topic>RGS Proteins - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Signal Transduction - drug effects</topic><topic>Sphingosine - analogs & derivatives</topic><topic>Sphingosine - metabolism</topic><topic>Sphingosine - pharmacology</topic><topic>Sphingosine-1-phosphate receptor</topic><topic>Up-Regulation - drug effects</topic><topic>VSMC</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hendriks-Balk, Mariëlle C.</creatorcontrib><creatorcontrib>Hajji, Najat</creatorcontrib><creatorcontrib>van Loenen, Pieter B.</creatorcontrib><creatorcontrib>Michel, Martin C.</creatorcontrib><creatorcontrib>Peters, Stephan L.M.</creatorcontrib><creatorcontrib>Alewijnse, Astrid E.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>European journal of pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hendriks-Balk, Mariëlle C.</au><au>Hajji, Najat</au><au>van Loenen, Pieter B.</au><au>Michel, Martin C.</au><au>Peters, Stephan L.M.</au><au>Alewijnse, Astrid E.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sphingosine-1-phosphate regulates RGS2 and RGS16 mRNA expression in vascular smooth muscle cells</atitle><jtitle>European journal of pharmacology</jtitle><addtitle>Eur J Pharmacol</addtitle><date>2009-03-15</date><risdate>2009</risdate><volume>606</volume><issue>1</issue><spage>25</spage><epage>31</epage><pages>25-31</pages><issn>0014-2999</issn><eissn>1879-0712</eissn><coden>EJPHAZ</coden><abstract>Regulator of G protein signalling (RGS) protein expression is altered under growth promoting conditions in vascular smooth muscle cells (VSMCs). Since sphingosine-1-phosphate (S1P) is an important growth stimulatory factor, we investigated whether stimulation of VSMCs with S1P results in alterations in mRNA expression levels of several RGS proteins and which signalling components are involved.
VSMCs were stimulated with S1P and mRNA expression levels of RGS2, RGS3, RGS4, RGS5 and RGS16 were measured by real-time polymerase chain reaction. S1P caused a time-dependent up-regulation of RGS2 and RGS16 mRNA expression. FTY720-P, a S1P
1/S1P
3–5 agonist, did not regulate RGS2 mRNA levels although it did up-regulate RGS16 mRNA expression. Pertussis toxin treatment revealed that the S1P-induced RGS16 expression was G
i/o-dependent whereas up-regulation of RGS2 mRNA was not. Phosphatidylinositol 3-kinase, protein kinase C and mitogen-activated protein kinase kinase apparently were not involved in the S1P-induced up-regulation of both RGS proteins.
The present study demonstrates that S1P induces RGS2 and RGS16 mRNA expression but uses distinct S1P receptor subtypes and signalling pathways to regulate expression of these RGS proteins.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>19374869</pmid><doi>10.1016/j.ejphar.2009.01.018</doi><tpages>7</tpages></addata></record> |
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| issn | 0014-2999 1879-0712 |
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| subjects | Animals Biological and medical sciences FTY720-P Gene Expression Regulation - drug effects Lysophospholipids - metabolism Lysophospholipids - pharmacology Male Medical sciences Muscle, Smooth, Vascular - cytology Muscle, Smooth, Vascular - drug effects Muscle, Smooth, Vascular - metabolism Pharmacology. Drug treatments Rats Rats, Wistar Receptors, Lysosphingolipid - metabolism Regulator of G protein signalling RGS Proteins - genetics RNA, Messenger - genetics RNA, Messenger - metabolism Signal Transduction - drug effects Sphingosine - analogs & derivatives Sphingosine - metabolism Sphingosine - pharmacology Sphingosine-1-phosphate receptor Up-Regulation - drug effects VSMC |
| title | Sphingosine-1-phosphate regulates RGS2 and RGS16 mRNA expression in vascular smooth muscle cells |
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