Detection of pathogenic Vibrio spp. in shellfish by using multiplex PCR and DNA microarrays

This study describes the development of a gene-specific DNA microarray coupled with multiplex PCR for the comprehensive detection of pathogenic vibrios that are natural inhabitants of warm coastal waters and shellfish. Multiplex PCR with vvh and viuB for Vibrio vulnificus, with ompU, toxR, tcpI, and...

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Veröffentlicht in:	Applied and Environmental Microbiology 2004-12, Vol.70 (12), p.7436-7444
Hauptverfasser:	Panicker, G, Call, D.R, Krug, M.J, Bej, A.K
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Bacteria Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Coasts Deoxyribonucleic acid DNA DNA probes Fish and seafood industries food contamination Food industries food microbiology Fundamental and applied biological sciences. Psychology Humans Marine Methods microarray technology microbial detection Microbiology Mollusca multiplex polymerase chain reaction Oligonucleotide Array Sequence Analysis - methods Ostreidae - microbiology oysters Pathogens polymerase chain reaction Polymerase Chain Reaction - methods Sensitivity and Specificity Shellfish Shellfish - microbiology Vibrio Vibrio - classification Vibrio - genetics Vibrio - isolation & purification Vibrio - pathogenicity Vibrio cholerae Vibrio cholerae - classification Vibrio cholerae - genetics Vibrio cholerae - isolation & purification Vibrio parahaemolyticus Vibrio parahaemolyticus - classification Vibrio parahaemolyticus - genetics Vibrio parahaemolyticus - isolation & purification Vibrio vulnificus Vibrio vulnificus - classification Vibrio vulnificus - genetics Vibrio vulnificus - isolation & purification
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