Electrophoresis microchip fabricated by a direct-printing process with end-channel amperometric detection
We describe the development of an electrophoresis microchip fabricated by a direct‐printing process, based on lamination of printed polyester films with end‐channel amperometric detection. The channel structures are defined by polyester (base and cover) and by a toner layer (walls). The polyester‐to...
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| Veröffentlicht in: | Electrophoresis 2004-11, Vol.25 (21-22), p.3832-3839 |
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| container_title | Electrophoresis |
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| creator | Tomazelli Coltro, Wendell K. Fracassi da Silva, José A. Torres da Silva, Heron D. Richter, Eduardo M. Furlan, Rogério Angnes, Lúcio do Lago, Claudimir L. Mazo, Luiz H. Carrilho, Emanuel |
| description | We describe the development of an electrophoresis microchip fabricated by a direct‐printing process, based on lamination of printed polyester films with end‐channel amperometric detection. The channel structures are defined by polyester (base and cover) and by a toner layer (walls). The polyester‐toner devices presented an electroosmotic flow (EOF) magnitude of ∼10‐5 cm2V‐1s‐1, which is generated by a polymeric mixture of the toner and polyester composition. The microelectrodes used for detection were produced combining this laser‐printer technology to compact discs. The performance of this device was evaluated by amperometric detection of iodide and ascorbate. The detection limits found were 500 nmol·L‐1 (135 amol) and 1.8 μmol·L‐1 (486 amol) for iodide and ascorbate, respectively. |
| doi_str_mv | 10.1002/elps.200406091 |
| format | Article |
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The channel structures are defined by polyester (base and cover) and by a toner layer (walls). The polyester‐toner devices presented an electroosmotic flow (EOF) magnitude of ∼10‐5 cm2V‐1s‐1, which is generated by a polymeric mixture of the toner and polyester composition. The microelectrodes used for detection were produced combining this laser‐printer technology to compact discs. The performance of this device was evaluated by amperometric detection of iodide and ascorbate. The detection limits found were 500 nmol·L‐1 (135 amol) and 1.8 μmol·L‐1 (486 amol) for iodide and ascorbate, respectively.</description><identifier>ISSN: 0173-0835</identifier><identifier>EISSN: 1522-2683</identifier><identifier>DOI: 10.1002/elps.200406091</identifier><identifier>PMID: 15565680</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Amperometric detection ; Ascorbic Acid - analysis ; Electrophoresis, Microchip - instrumentation ; Equipment Design ; Iodides - analysis ; Manufactured Materials ; Microchip ; Microelectrodes ; Microfluidic Analytical Techniques - instrumentation ; Miniaturization ; Polyesters</subject><ispartof>Electrophoresis, 2004-11, Vol.25 (21-22), p.3832-3839</ispartof><rights>Copyright © 2004 WILEY‐VCH Verlag GmbH & Co. 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The channel structures are defined by polyester (base and cover) and by a toner layer (walls). The polyester‐toner devices presented an electroosmotic flow (EOF) magnitude of ∼10‐5 cm2V‐1s‐1, which is generated by a polymeric mixture of the toner and polyester composition. The microelectrodes used for detection were produced combining this laser‐printer technology to compact discs. The performance of this device was evaluated by amperometric detection of iodide and ascorbate. The detection limits found were 500 nmol·L‐1 (135 amol) and 1.8 μmol·L‐1 (486 amol) for iodide and ascorbate, respectively.</description><subject>Amperometric detection</subject><subject>Ascorbic Acid - analysis</subject><subject>Electrophoresis, Microchip - instrumentation</subject><subject>Equipment Design</subject><subject>Iodides - analysis</subject><subject>Manufactured Materials</subject><subject>Microchip</subject><subject>Microelectrodes</subject><subject>Microfluidic Analytical Techniques - instrumentation</subject><subject>Miniaturization</subject><subject>Polyesters</subject><issn>0173-0835</issn><issn>1522-2683</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PGzEURa0KVFLKtkvkFbsJz-PxeGZZaBqQQlupIJaWx35uTOcLeyKaf1-jRMCO1ducc3XfJeQLgzkDyM-xHeM8ByighJp9IDMm8jzLy4ofkBkwyTOouDgin2J8gITVRfGRHDEhSlFWMCN-0aKZwjCuh4DRR9p5Ewaz9iN1ugne6AktbbZUU-tDQrMx-H7y_R86Jg5jpE9-WlPsbWbWuu-xpbobMQwdTkmnFqdk-aH_TA6dbiOe7O8xufu-uL28ylY_l9eXX1eZ4RVjmWNMg7M501bXRQmGuzLXtSxlASz1tyClBG0aa1xRV1I6VwnmOGCDtkHBj8nZLjf1e9xgnFTno8G21T0Om6hKyYoKeJ7A-Q5MD8cY0Kn0WqfDVjFQz-Oq53HVy7hJON0nb5oO7Su-XzMB9Q548i1u34lTi9Wv32_Ds53r44T_Xlwd_qbGXAp1_2Opbu6r4vbi5psS_D_TPJgU</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Tomazelli Coltro, Wendell K.</creator><creator>Fracassi da Silva, José A.</creator><creator>Torres da Silva, Heron D.</creator><creator>Richter, Eduardo M.</creator><creator>Furlan, Rogério</creator><creator>Angnes, Lúcio</creator><creator>do Lago, Claudimir L.</creator><creator>Mazo, Luiz H.</creator><creator>Carrilho, Emanuel</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Electrophoresis microchip fabricated by a direct-printing process with end-channel amperometric detection</title><author>Tomazelli Coltro, Wendell K. ; Fracassi da Silva, José A. ; Torres da Silva, Heron D. ; Richter, Eduardo M. ; Furlan, Rogério ; Angnes, Lúcio ; do Lago, Claudimir L. ; Mazo, Luiz H. ; Carrilho, Emanuel</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3811-f11a0fd21ada9460c3f62a9767401049d07770acbdcf49877ff851f30ebedbe53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amperometric detection</topic><topic>Ascorbic Acid - analysis</topic><topic>Electrophoresis, Microchip - instrumentation</topic><topic>Equipment Design</topic><topic>Iodides - analysis</topic><topic>Manufactured Materials</topic><topic>Microchip</topic><topic>Microelectrodes</topic><topic>Microfluidic Analytical Techniques - instrumentation</topic><topic>Miniaturization</topic><topic>Polyesters</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tomazelli Coltro, Wendell K.</creatorcontrib><creatorcontrib>Fracassi da Silva, José A.</creatorcontrib><creatorcontrib>Torres da Silva, Heron D.</creatorcontrib><creatorcontrib>Richter, Eduardo M.</creatorcontrib><creatorcontrib>Furlan, Rogério</creatorcontrib><creatorcontrib>Angnes, Lúcio</creatorcontrib><creatorcontrib>do Lago, Claudimir L.</creatorcontrib><creatorcontrib>Mazo, Luiz H.</creatorcontrib><creatorcontrib>Carrilho, Emanuel</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Electrophoresis</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tomazelli Coltro, Wendell K.</au><au>Fracassi da Silva, José A.</au><au>Torres da Silva, Heron D.</au><au>Richter, Eduardo M.</au><au>Furlan, Rogério</au><au>Angnes, Lúcio</au><au>do Lago, Claudimir L.</au><au>Mazo, Luiz H.</au><au>Carrilho, Emanuel</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Electrophoresis microchip fabricated by a direct-printing process with end-channel amperometric detection</atitle><jtitle>Electrophoresis</jtitle><addtitle>ELECTROPHORESIS</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>25</volume><issue>21-22</issue><spage>3832</spage><epage>3839</epage><pages>3832-3839</pages><issn>0173-0835</issn><eissn>1522-2683</eissn><abstract>We describe the development of an electrophoresis microchip fabricated by a direct‐printing process, based on lamination of printed polyester films with end‐channel amperometric detection. The channel structures are defined by polyester (base and cover) and by a toner layer (walls). The polyester‐toner devices presented an electroosmotic flow (EOF) magnitude of ∼10‐5 cm2V‐1s‐1, which is generated by a polymeric mixture of the toner and polyester composition. The microelectrodes used for detection were produced combining this laser‐printer technology to compact discs. The performance of this device was evaluated by amperometric detection of iodide and ascorbate. The detection limits found were 500 nmol·L‐1 (135 amol) and 1.8 μmol·L‐1 (486 amol) for iodide and ascorbate, respectively.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>15565680</pmid><doi>10.1002/elps.200406091</doi><tpages>8</tpages></addata></record> |
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| ispartof | Electrophoresis, 2004-11, Vol.25 (21-22), p.3832-3839 |
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| language | eng |
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| source | Wiley Online Library - AutoHoldings Journals; MEDLINE |
| subjects | Amperometric detection Ascorbic Acid - analysis Electrophoresis, Microchip - instrumentation Equipment Design Iodides - analysis Manufactured Materials Microchip Microelectrodes Microfluidic Analytical Techniques - instrumentation Miniaturization Polyesters |
| title | Electrophoresis microchip fabricated by a direct-printing process with end-channel amperometric detection |
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