Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical Cord
The Wharton's jelly of the umbilical cord contains mucoid connective tissue and fibroblast‐like cells. Using flow cytometric analysis, we found that mesenchymal cells isolated from the umbilical cord express matrix receptors (CD44, CD105) and integrin markers (CD29, CD51) but not hematopoietic...
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| Veröffentlicht in: | Stem cells (Dayton, Ohio) Ohio), 2004-12, Vol.22 (7), p.1330-1337 |
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| creator | Wang, Hwai‐Shi Hung, Shih‐Chieh Peng, Shu‐Tine Huang, Chun‐Chieh Wei, Hung‐Mu Guo, Yi‐Jhih Fu, Yu‐Show Lai, Mei‐Chun Chen, Chin‐Chang |
| description | The Wharton's jelly of the umbilical cord contains mucoid connective tissue and fibroblast‐like cells. Using flow cytometric analysis, we found that mesenchymal cells isolated from the umbilical cord express matrix receptors (CD44, CD105) and integrin markers (CD29, CD51) but not hematopoietic lineage markers (CD34, CD45). Interestingly, these cells also express significant amounts of mesenchymal stem cell markers (SH2, SH3). We therefore investigated the potential of these cells to differentiate into cardiomyocytes by treating them with 5‐azacytidine or by culturing them in cardiomyocyte‐conditioned medium and found that both sets of conditions resulted in the expression of cardiomyocyte markers, namely N‐cadherin and cardiac troponin I. We also showed that these cells have multilineage potential and that, under suitable culture conditions, are able to differentiate into cells of the adipogenic and osteogenic lineages. These findings may have a significant impact on studies of early human cardiac differentiation, functional genomics, pharmacological testing, cell therapy, and tissue engineering by helping to eliminate worrying ethical and technical issues. |
| doi_str_mv | 10.1634/stemcells.2004-0013 |
| format | Article |
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Using flow cytometric analysis, we found that mesenchymal cells isolated from the umbilical cord express matrix receptors (CD44, CD105) and integrin markers (CD29, CD51) but not hematopoietic lineage markers (CD34, CD45). Interestingly, these cells also express significant amounts of mesenchymal stem cell markers (SH2, SH3). We therefore investigated the potential of these cells to differentiate into cardiomyocytes by treating them with 5‐azacytidine or by culturing them in cardiomyocyte‐conditioned medium and found that both sets of conditions resulted in the expression of cardiomyocyte markers, namely N‐cadherin and cardiac troponin I. We also showed that these cells have multilineage potential and that, under suitable culture conditions, are able to differentiate into cells of the adipogenic and osteogenic lineages. These findings may have a significant impact on studies of early human cardiac differentiation, functional genomics, pharmacological testing, cell therapy, and tissue engineering by helping to eliminate worrying ethical and technical issues.</description><identifier>ISSN: 1066-5099</identifier><identifier>EISSN: 1549-4918</identifier><identifier>DOI: 10.1634/stemcells.2004-0013</identifier><identifier>PMID: 15579650</identifier><language>eng</language><publisher>Bristol: John Wiley & Sons, Ltd</publisher><subject>Adipocytes - cytology ; Adipocytes - metabolism ; Antigens, CD ; Antigens, CD34 - biosynthesis ; Blotting, Western ; Cell Differentiation ; Cells, Cultured ; Chondrocytes - metabolism ; Endoglin ; Flow Cytometry ; Humans ; Hyaluronan Receptors - biosynthesis ; Immunohistochemistry ; Leukocyte Common Antigens - biosynthesis ; Mesenchymal stem cells ; Mesenchymal Stromal Cells - cytology ; Multipotent cells ; Receptors, Cell Surface ; Reverse Transcriptase Polymerase Chain Reaction ; RNA - metabolism ; Umbilical cord ; Umbilical Cord - cytology ; Vascular Cell Adhesion Molecule-1 - biosynthesis ; Wharton's jelly</subject><ispartof>Stem cells (Dayton, Ohio), 2004-12, Vol.22 (7), p.1330-1337</ispartof><rights>Copyright © 2004 AlphaMed Press</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4970-ec8959c2c948736b8c5c5c3fba0542b2c42da02dead7269bc5eb429548ffb93c3</citedby><cites>FETCH-LOGICAL-c4970-ec8959c2c948736b8c5c5c3fba0542b2c42da02dead7269bc5eb429548ffb93c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15579650$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wang, Hwai‐Shi</creatorcontrib><creatorcontrib>Hung, Shih‐Chieh</creatorcontrib><creatorcontrib>Peng, Shu‐Tine</creatorcontrib><creatorcontrib>Huang, Chun‐Chieh</creatorcontrib><creatorcontrib>Wei, Hung‐Mu</creatorcontrib><creatorcontrib>Guo, Yi‐Jhih</creatorcontrib><creatorcontrib>Fu, Yu‐Show</creatorcontrib><creatorcontrib>Lai, Mei‐Chun</creatorcontrib><creatorcontrib>Chen, Chin‐Chang</creatorcontrib><title>Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical Cord</title><title>Stem cells (Dayton, Ohio)</title><addtitle>Stem Cells</addtitle><description>The Wharton's jelly of the umbilical cord contains mucoid connective tissue and fibroblast‐like cells. Using flow cytometric analysis, we found that mesenchymal cells isolated from the umbilical cord express matrix receptors (CD44, CD105) and integrin markers (CD29, CD51) but not hematopoietic lineage markers (CD34, CD45). Interestingly, these cells also express significant amounts of mesenchymal stem cell markers (SH2, SH3). We therefore investigated the potential of these cells to differentiate into cardiomyocytes by treating them with 5‐azacytidine or by culturing them in cardiomyocyte‐conditioned medium and found that both sets of conditions resulted in the expression of cardiomyocyte markers, namely N‐cadherin and cardiac troponin I. We also showed that these cells have multilineage potential and that, under suitable culture conditions, are able to differentiate into cells of the adipogenic and osteogenic lineages. These findings may have a significant impact on studies of early human cardiac differentiation, functional genomics, pharmacological testing, cell therapy, and tissue engineering by helping to eliminate worrying ethical and technical issues.</description><subject>Adipocytes - cytology</subject><subject>Adipocytes - metabolism</subject><subject>Antigens, CD</subject><subject>Antigens, CD34 - biosynthesis</subject><subject>Blotting, Western</subject><subject>Cell Differentiation</subject><subject>Cells, Cultured</subject><subject>Chondrocytes - metabolism</subject><subject>Endoglin</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Hyaluronan Receptors - biosynthesis</subject><subject>Immunohistochemistry</subject><subject>Leukocyte Common Antigens - biosynthesis</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stromal Cells - cytology</subject><subject>Multipotent cells</subject><subject>Receptors, Cell Surface</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA - metabolism</subject><subject>Umbilical cord</subject><subject>Umbilical Cord - cytology</subject><subject>Vascular Cell Adhesion Molecule-1 - biosynthesis</subject><subject>Wharton's jelly</subject><issn>1066-5099</issn><issn>1549-4918</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkE1LwzAYgIMobk5_gSA56akz323wJGW6yYaHbXgMaZqySj9m0yL996Zu6FHJ4Q0vz_scHgCuMZpiQdm9a21pbFG4KUGIBQhhegLGmDMZMImjU_9HQgQcSTkCF869e4LxKDoHI8x5KAVHY7BYWWcrs-tLXcC1N8J4UMK8gu3Owredbtq6unPwxa97WGff63lX6gpuyyQvcuMP47pJL8FZpgtnr45zArZPs008D5avz4v4cRkYJkMUWBNJLg0xkkUhFUlkuH80SzTijCTEMJJqRFKr05AImRhuE0YkZ1GWJZIaOgG3B---qT8661pV5m7ooCtbd06JEDNBMf0TxFJSGknkQXoATVM719hM7Zu81E2vMFJDavWTWg2p1ZDaX90c9V1S2vT35tjWAw8H4DMvbP8fp1pvZitCvBzRL37Yjo0</recordid><startdate>200412</startdate><enddate>200412</enddate><creator>Wang, Hwai‐Shi</creator><creator>Hung, Shih‐Chieh</creator><creator>Peng, Shu‐Tine</creator><creator>Huang, Chun‐Chieh</creator><creator>Wei, Hung‐Mu</creator><creator>Guo, Yi‐Jhih</creator><creator>Fu, Yu‐Show</creator><creator>Lai, Mei‐Chun</creator><creator>Chen, Chin‐Chang</creator><general>John Wiley & Sons, Ltd</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>200412</creationdate><title>Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical Cord</title><author>Wang, Hwai‐Shi ; Hung, Shih‐Chieh ; Peng, Shu‐Tine ; Huang, Chun‐Chieh ; Wei, Hung‐Mu ; Guo, Yi‐Jhih ; Fu, Yu‐Show ; Lai, Mei‐Chun ; Chen, Chin‐Chang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4970-ec8959c2c948736b8c5c5c3fba0542b2c42da02dead7269bc5eb429548ffb93c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adipocytes - cytology</topic><topic>Adipocytes - metabolism</topic><topic>Antigens, CD</topic><topic>Antigens, CD34 - biosynthesis</topic><topic>Blotting, Western</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Chondrocytes - metabolism</topic><topic>Endoglin</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Hyaluronan Receptors - biosynthesis</topic><topic>Immunohistochemistry</topic><topic>Leukocyte Common Antigens - biosynthesis</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stromal Cells - cytology</topic><topic>Multipotent cells</topic><topic>Receptors, Cell Surface</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA - metabolism</topic><topic>Umbilical cord</topic><topic>Umbilical Cord - cytology</topic><topic>Vascular Cell Adhesion Molecule-1 - biosynthesis</topic><topic>Wharton's jelly</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wang, Hwai‐Shi</creatorcontrib><creatorcontrib>Hung, Shih‐Chieh</creatorcontrib><creatorcontrib>Peng, Shu‐Tine</creatorcontrib><creatorcontrib>Huang, Chun‐Chieh</creatorcontrib><creatorcontrib>Wei, Hung‐Mu</creatorcontrib><creatorcontrib>Guo, Yi‐Jhih</creatorcontrib><creatorcontrib>Fu, Yu‐Show</creatorcontrib><creatorcontrib>Lai, Mei‐Chun</creatorcontrib><creatorcontrib>Chen, Chin‐Chang</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Stem cells (Dayton, Ohio)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wang, Hwai‐Shi</au><au>Hung, Shih‐Chieh</au><au>Peng, Shu‐Tine</au><au>Huang, Chun‐Chieh</au><au>Wei, Hung‐Mu</au><au>Guo, Yi‐Jhih</au><au>Fu, Yu‐Show</au><au>Lai, Mei‐Chun</au><au>Chen, Chin‐Chang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical Cord</atitle><jtitle>Stem cells (Dayton, Ohio)</jtitle><addtitle>Stem Cells</addtitle><date>2004-12</date><risdate>2004</risdate><volume>22</volume><issue>7</issue><spage>1330</spage><epage>1337</epage><pages>1330-1337</pages><issn>1066-5099</issn><eissn>1549-4918</eissn><abstract>The Wharton's jelly of the umbilical cord contains mucoid connective tissue and fibroblast‐like cells. Using flow cytometric analysis, we found that mesenchymal cells isolated from the umbilical cord express matrix receptors (CD44, CD105) and integrin markers (CD29, CD51) but not hematopoietic lineage markers (CD34, CD45). Interestingly, these cells also express significant amounts of mesenchymal stem cell markers (SH2, SH3). We therefore investigated the potential of these cells to differentiate into cardiomyocytes by treating them with 5‐azacytidine or by culturing them in cardiomyocyte‐conditioned medium and found that both sets of conditions resulted in the expression of cardiomyocyte markers, namely N‐cadherin and cardiac troponin I. We also showed that these cells have multilineage potential and that, under suitable culture conditions, are able to differentiate into cells of the adipogenic and osteogenic lineages. These findings may have a significant impact on studies of early human cardiac differentiation, functional genomics, pharmacological testing, cell therapy, and tissue engineering by helping to eliminate worrying ethical and technical issues.</abstract><cop>Bristol</cop><pub>John Wiley & Sons, Ltd</pub><pmid>15579650</pmid><doi>10.1634/stemcells.2004-0013</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | Adipocytes - cytology Adipocytes - metabolism Antigens, CD Antigens, CD34 - biosynthesis Blotting, Western Cell Differentiation Cells, Cultured Chondrocytes - metabolism Endoglin Flow Cytometry Humans Hyaluronan Receptors - biosynthesis Immunohistochemistry Leukocyte Common Antigens - biosynthesis Mesenchymal stem cells Mesenchymal Stromal Cells - cytology Multipotent cells Receptors, Cell Surface Reverse Transcriptase Polymerase Chain Reaction RNA - metabolism Umbilical cord Umbilical Cord - cytology Vascular Cell Adhesion Molecule-1 - biosynthesis Wharton's jelly |
| title | Mesenchymal Stem Cells in the Wharton's Jelly of the Human Umbilical Cord |
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