Spred-1 Negatively Regulates Interleukin-3-mediated ERK/Mitogen-activated Protein (MAP) Kinase Activation in Hematopoietic Cells
Sprouty/Spred family proteins have been identified as negative regulators of growth factor-induced ERK/mitogen-activated protein (MAP) kinase activation. However, it has not been clarified whether these proteins regulate cytokine-induced ERK activity. We found that Spred-1 is highly expressed in int...
Gespeichert in:
| Veröffentlicht in: | The Journal of biological chemistry 2004-12, Vol.279 (50), p.52543-52551 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 52551 |
|---|---|
| container_issue | 50 |
| container_start_page | 52543 |
| container_title | The Journal of biological chemistry |
| container_volume | 279 |
| creator | Nonami, Atsushi Kato, Reiko Taniguchi, Koji Yoshiga, Daigo Taketomi, Takaharu Fukuyama, Satoru Harada, Mine Sasaki, Atsuo Yoshimura, Akihiko |
| description | Sprouty/Spred family proteins have been identified as negative regulators of growth factor-induced ERK/mitogen-activated protein
(MAP) kinase activation. However, it has not been clarified whether these proteins regulate cytokine-induced ERK activity.
We found that Spred-1 is highly expressed in interleukin-3 (IL-3)-dependent hematopoietic cell lines and bone marrow-derived
mast cells. To investigate the roles of Spred-1 in hematopoiesis, we expressed wild-type Spred-1 and a dominant negative form
of Spred-1, ÎC-Spred, in IL-3- and stem cell factor (SCF)-dependent cell lines as well as hematopoietic progenitor cells from
mouse bone marrow by retrovirus gene transfer. In IL-3-dependent Ba/F3 cells expressing c-kit, forced expression of Spred-1
resulted in a reduced proliferation rate and ERK activation in response to not only SCF but also IL-3. In contrast, ÎC-Spred
augmented IL-3-induced cell proliferation and ERK activation. Wild-type Spred-1 inhibited colony formation of bone marrow
cells in the presence of cytokines, whereas ÎC-Spred-1 expression enhanced colony formation. Augmentation of ERK activation
and proliferation in response to IL-3 was also observed in Spred-1-deficient bone marrow-derived mast cells. These data suggest
that Spred-1 negatively regulates hematopoiesis by suppressing not only SCF-induced but also IL-3-induced ERK activation. |
| doi_str_mv | 10.1074/jbc.M405189200 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67146139</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>17793514</sourcerecordid><originalsourceid>FETCH-LOGICAL-c459t-d52e12e7ff42e28d4154134fa16dd51c4f694dd88fe5f8f37aafd5f941241a6b3</originalsourceid><addsrcrecordid>eNqFkU1v1DAQhi0EokvhyhH5gBA9ZOvxRxIfV6tCq3ZLVUDiZnnj8a5LEi9xAuqtPx3DrtRj5zLSvM-MZuYl5C2wObBKnt6tm_lKMgW15ow9IzNgtSiEgh_PyYwxDoXmqj4ir1K6YzmkhpfkCJQsVQ1qRh6-7gZ0BdBr3Ngx_Mb2nt7iZmrtiIle9CMOLU4_Q1-IokMXctnRs9vL01UY4wb7wja563_1Zogjhp5-XC1uTuhl6G1CutjLIfY0S-fY2THuYsAxNHSJbZtekxfetgnfHPIx-f7p7NvyvLj68vliubgqGqn0WDjFEThW3kuOvHYynwBCegulcwoa6Ustnatrj8rXXlTWeqe8lsAl2HItjsmH_dzdEH9NmEbThdTkDWyPcUqmrECWIPSTIFSVzv-VGZzvwWaIKQ3ozW4InR3uDTDzzxyTzTGP5uSGd4fJ0zr_8hE_uJGB93tgGzbbP2FAsw6x2WJneKWNYkZxJYX4Cy_SlxY</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17793514</pqid></control><display><type>article</type><title>Spred-1 Negatively Regulates Interleukin-3-mediated ERK/Mitogen-activated Protein (MAP) Kinase Activation in Hematopoietic Cells</title><source>MEDLINE</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Nonami, Atsushi ; Kato, Reiko ; Taniguchi, Koji ; Yoshiga, Daigo ; Taketomi, Takaharu ; Fukuyama, Satoru ; Harada, Mine ; Sasaki, Atsuo ; Yoshimura, Akihiko</creator><creatorcontrib>Nonami, Atsushi ; Kato, Reiko ; Taniguchi, Koji ; Yoshiga, Daigo ; Taketomi, Takaharu ; Fukuyama, Satoru ; Harada, Mine ; Sasaki, Atsuo ; Yoshimura, Akihiko</creatorcontrib><description>Sprouty/Spred family proteins have been identified as negative regulators of growth factor-induced ERK/mitogen-activated protein
(MAP) kinase activation. However, it has not been clarified whether these proteins regulate cytokine-induced ERK activity.
We found that Spred-1 is highly expressed in interleukin-3 (IL-3)-dependent hematopoietic cell lines and bone marrow-derived
mast cells. To investigate the roles of Spred-1 in hematopoiesis, we expressed wild-type Spred-1 and a dominant negative form
of Spred-1, ÎC-Spred, in IL-3- and stem cell factor (SCF)-dependent cell lines as well as hematopoietic progenitor cells from
mouse bone marrow by retrovirus gene transfer. In IL-3-dependent Ba/F3 cells expressing c-kit, forced expression of Spred-1
resulted in a reduced proliferation rate and ERK activation in response to not only SCF but also IL-3. In contrast, ÎC-Spred
augmented IL-3-induced cell proliferation and ERK activation. Wild-type Spred-1 inhibited colony formation of bone marrow
cells in the presence of cytokines, whereas ÎC-Spred-1 expression enhanced colony formation. Augmentation of ERK activation
and proliferation in response to IL-3 was also observed in Spred-1-deficient bone marrow-derived mast cells. These data suggest
that Spred-1 negatively regulates hematopoiesis by suppressing not only SCF-induced but also IL-3-induced ERK activation.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M405189200</identifier><identifier>PMID: 15465815</identifier><language>eng</language><publisher>United States: American Society for Biochemistry and Molecular Biology</publisher><subject>Adaptor Proteins, Signal Transducing ; Animals ; Cell Line ; Colony-Forming Units Assay ; Enzyme Activation ; Extracellular Signal-Regulated MAP Kinases - metabolism ; Gene Expression ; Hematopoiesis ; Hematopoietic Stem Cells - metabolism ; Humans ; In Vitro Techniques ; Interleukin-3 - metabolism ; MAP Kinase Signaling System ; Mice ; Mice, Inbred C57BL ; Mice, Knockout ; Repressor Proteins - genetics ; Repressor Proteins - metabolism ; Stem Cell Factor - metabolism</subject><ispartof>The Journal of biological chemistry, 2004-12, Vol.279 (50), p.52543-52551</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c459t-d52e12e7ff42e28d4154134fa16dd51c4f694dd88fe5f8f37aafd5f941241a6b3</citedby><cites>FETCH-LOGICAL-c459t-d52e12e7ff42e28d4154134fa16dd51c4f694dd88fe5f8f37aafd5f941241a6b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15465815$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Nonami, Atsushi</creatorcontrib><creatorcontrib>Kato, Reiko</creatorcontrib><creatorcontrib>Taniguchi, Koji</creatorcontrib><creatorcontrib>Yoshiga, Daigo</creatorcontrib><creatorcontrib>Taketomi, Takaharu</creatorcontrib><creatorcontrib>Fukuyama, Satoru</creatorcontrib><creatorcontrib>Harada, Mine</creatorcontrib><creatorcontrib>Sasaki, Atsuo</creatorcontrib><creatorcontrib>Yoshimura, Akihiko</creatorcontrib><title>Spred-1 Negatively Regulates Interleukin-3-mediated ERK/Mitogen-activated Protein (MAP) Kinase Activation in Hematopoietic Cells</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Sprouty/Spred family proteins have been identified as negative regulators of growth factor-induced ERK/mitogen-activated protein
(MAP) kinase activation. However, it has not been clarified whether these proteins regulate cytokine-induced ERK activity.
We found that Spred-1 is highly expressed in interleukin-3 (IL-3)-dependent hematopoietic cell lines and bone marrow-derived
mast cells. To investigate the roles of Spred-1 in hematopoiesis, we expressed wild-type Spred-1 and a dominant negative form
of Spred-1, ÎC-Spred, in IL-3- and stem cell factor (SCF)-dependent cell lines as well as hematopoietic progenitor cells from
mouse bone marrow by retrovirus gene transfer. In IL-3-dependent Ba/F3 cells expressing c-kit, forced expression of Spred-1
resulted in a reduced proliferation rate and ERK activation in response to not only SCF but also IL-3. In contrast, ÎC-Spred
augmented IL-3-induced cell proliferation and ERK activation. Wild-type Spred-1 inhibited colony formation of bone marrow
cells in the presence of cytokines, whereas ÎC-Spred-1 expression enhanced colony formation. Augmentation of ERK activation
and proliferation in response to IL-3 was also observed in Spred-1-deficient bone marrow-derived mast cells. These data suggest
that Spred-1 negatively regulates hematopoiesis by suppressing not only SCF-induced but also IL-3-induced ERK activation.</description><subject>Adaptor Proteins, Signal Transducing</subject><subject>Animals</subject><subject>Cell Line</subject><subject>Colony-Forming Units Assay</subject><subject>Enzyme Activation</subject><subject>Extracellular Signal-Regulated MAP Kinases - metabolism</subject><subject>Gene Expression</subject><subject>Hematopoiesis</subject><subject>Hematopoietic Stem Cells - metabolism</subject><subject>Humans</subject><subject>In Vitro Techniques</subject><subject>Interleukin-3 - metabolism</subject><subject>MAP Kinase Signaling System</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Knockout</subject><subject>Repressor Proteins - genetics</subject><subject>Repressor Proteins - metabolism</subject><subject>Stem Cell Factor - metabolism</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EokvhyhH5gBA9ZOvxRxIfV6tCq3ZLVUDiZnnj8a5LEi9xAuqtPx3DrtRj5zLSvM-MZuYl5C2wObBKnt6tm_lKMgW15ow9IzNgtSiEgh_PyYwxDoXmqj4ir1K6YzmkhpfkCJQsVQ1qRh6-7gZ0BdBr3Ngx_Mb2nt7iZmrtiIle9CMOLU4_Q1-IokMXctnRs9vL01UY4wb7wja563_1Zogjhp5-XC1uTuhl6G1CutjLIfY0S-fY2THuYsAxNHSJbZtekxfetgnfHPIx-f7p7NvyvLj68vliubgqGqn0WDjFEThW3kuOvHYynwBCegulcwoa6Ustnatrj8rXXlTWeqe8lsAl2HItjsmH_dzdEH9NmEbThdTkDWyPcUqmrECWIPSTIFSVzv-VGZzvwWaIKQ3ozW4InR3uDTDzzxyTzTGP5uSGd4fJ0zr_8hE_uJGB93tgGzbbP2FAsw6x2WJneKWNYkZxJYX4Cy_SlxY</recordid><startdate>20041210</startdate><enddate>20041210</enddate><creator>Nonami, Atsushi</creator><creator>Kato, Reiko</creator><creator>Taniguchi, Koji</creator><creator>Yoshiga, Daigo</creator><creator>Taketomi, Takaharu</creator><creator>Fukuyama, Satoru</creator><creator>Harada, Mine</creator><creator>Sasaki, Atsuo</creator><creator>Yoshimura, Akihiko</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20041210</creationdate><title>Spred-1 Negatively Regulates Interleukin-3-mediated ERK/Mitogen-activated Protein (MAP) Kinase Activation in Hematopoietic Cells</title><author>Nonami, Atsushi ; Kato, Reiko ; Taniguchi, Koji ; Yoshiga, Daigo ; Taketomi, Takaharu ; Fukuyama, Satoru ; Harada, Mine ; Sasaki, Atsuo ; Yoshimura, Akihiko</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c459t-d52e12e7ff42e28d4154134fa16dd51c4f694dd88fe5f8f37aafd5f941241a6b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adaptor Proteins, Signal Transducing</topic><topic>Animals</topic><topic>Cell Line</topic><topic>Colony-Forming Units Assay</topic><topic>Enzyme Activation</topic><topic>Extracellular Signal-Regulated MAP Kinases - metabolism</topic><topic>Gene Expression</topic><topic>Hematopoiesis</topic><topic>Hematopoietic Stem Cells - metabolism</topic><topic>Humans</topic><topic>In Vitro Techniques</topic><topic>Interleukin-3 - metabolism</topic><topic>MAP Kinase Signaling System</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Knockout</topic><topic>Repressor Proteins - genetics</topic><topic>Repressor Proteins - metabolism</topic><topic>Stem Cell Factor - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nonami, Atsushi</creatorcontrib><creatorcontrib>Kato, Reiko</creatorcontrib><creatorcontrib>Taniguchi, Koji</creatorcontrib><creatorcontrib>Yoshiga, Daigo</creatorcontrib><creatorcontrib>Taketomi, Takaharu</creatorcontrib><creatorcontrib>Fukuyama, Satoru</creatorcontrib><creatorcontrib>Harada, Mine</creatorcontrib><creatorcontrib>Sasaki, Atsuo</creatorcontrib><creatorcontrib>Yoshimura, Akihiko</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nonami, Atsushi</au><au>Kato, Reiko</au><au>Taniguchi, Koji</au><au>Yoshiga, Daigo</au><au>Taketomi, Takaharu</au><au>Fukuyama, Satoru</au><au>Harada, Mine</au><au>Sasaki, Atsuo</au><au>Yoshimura, Akihiko</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Spred-1 Negatively Regulates Interleukin-3-mediated ERK/Mitogen-activated Protein (MAP) Kinase Activation in Hematopoietic Cells</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2004-12-10</date><risdate>2004</risdate><volume>279</volume><issue>50</issue><spage>52543</spage><epage>52551</epage><pages>52543-52551</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Sprouty/Spred family proteins have been identified as negative regulators of growth factor-induced ERK/mitogen-activated protein
(MAP) kinase activation. However, it has not been clarified whether these proteins regulate cytokine-induced ERK activity.
We found that Spred-1 is highly expressed in interleukin-3 (IL-3)-dependent hematopoietic cell lines and bone marrow-derived
mast cells. To investigate the roles of Spred-1 in hematopoiesis, we expressed wild-type Spred-1 and a dominant negative form
of Spred-1, ÎC-Spred, in IL-3- and stem cell factor (SCF)-dependent cell lines as well as hematopoietic progenitor cells from
mouse bone marrow by retrovirus gene transfer. In IL-3-dependent Ba/F3 cells expressing c-kit, forced expression of Spred-1
resulted in a reduced proliferation rate and ERK activation in response to not only SCF but also IL-3. In contrast, ÎC-Spred
augmented IL-3-induced cell proliferation and ERK activation. Wild-type Spred-1 inhibited colony formation of bone marrow
cells in the presence of cytokines, whereas ÎC-Spred-1 expression enhanced colony formation. Augmentation of ERK activation
and proliferation in response to IL-3 was also observed in Spred-1-deficient bone marrow-derived mast cells. These data suggest
that Spred-1 negatively regulates hematopoiesis by suppressing not only SCF-induced but also IL-3-induced ERK activation.</abstract><cop>United States</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>15465815</pmid><doi>10.1074/jbc.M405189200</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 2004-12, Vol.279 (50), p.52543-52551 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67146139 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Adaptor Proteins, Signal Transducing Animals Cell Line Colony-Forming Units Assay Enzyme Activation Extracellular Signal-Regulated MAP Kinases - metabolism Gene Expression Hematopoiesis Hematopoietic Stem Cells - metabolism Humans In Vitro Techniques Interleukin-3 - metabolism MAP Kinase Signaling System Mice Mice, Inbred C57BL Mice, Knockout Repressor Proteins - genetics Repressor Proteins - metabolism Stem Cell Factor - metabolism |
| title | Spred-1 Negatively Regulates Interleukin-3-mediated ERK/Mitogen-activated Protein (MAP) Kinase Activation in Hematopoietic Cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T15%3A08%3A21IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Spred-1%20Negatively%20Regulates%20Interleukin-3-mediated%20ERK/Mitogen-activated%20Protein%20(MAP)%20Kinase%20Activation%20in%20Hematopoietic%20Cells&rft.jtitle=The%20Journal%20of%20biological%20chemistry&rft.au=Nonami,%20Atsushi&rft.date=2004-12-10&rft.volume=279&rft.issue=50&rft.spage=52543&rft.epage=52551&rft.pages=52543-52551&rft.issn=0021-9258&rft.eissn=1083-351X&rft_id=info:doi/10.1074/jbc.M405189200&rft_dat=%3Cproquest_cross%3E17793514%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17793514&rft_id=info:pmid/15465815&rfr_iscdi=true |