Reversible Transdifferentiation of Secretory Epithelial Cells into Adipocytes in the Mammary Gland
Mammalian breast adipose tissue is replaced by a milk-secreting gland during pregnancy; the reverse process takes place upon interruption of lactation. Morphological and bromodeoxyuridine studies provide indirect evidence that mouse mammary adipocytes transform into secretory epithelial cells during...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2004-11, Vol.101 (48), p.16801-16806 |
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| container_issue | 48 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Morroni, Manrico Giordano, Antonio Zingaretti, Maria Cristina Boiani, Romina de Matteis, Rita Kahn, Barbara B. Nisoli, Enzo Tonello, Cristina Pisoschi, Catalina Luchetti, Michele M. Marelli, Mariella Cinti, Saverio Hay, Elizabeth D. |
| description | Mammalian breast adipose tissue is replaced by a milk-secreting gland during pregnancy; the reverse process takes place upon interruption of lactation. Morphological and bromodeoxyuridine studies provide indirect evidence that mouse mammary adipocytes transform into secretory epithelial cells during pregnancy and revert to adipocytes after lactation. By using the Cre-loxP recombination system we show that the mammary gland of whey acidic protein (WAP)-Cre/R26R mice, in which secretory epithelial cells express the lacZ gene during pregnancy, contains labeled adipocytes during involution. Conversely, adipocyte P2-Cre/R26R mice, in which adipocytes are labeled before pregnancy, contain labeled secretory epithelial cells during pregnancy. We conclude that reversible adipocyte-to-epithelium and epithelium-to-adipocyte transdifferentiation occurs in the mammary gland of adult mice during pregnancy and lactation. |
| doi_str_mv | 10.1073/pnas.0407647101 |
| format | Article |
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Morphological and bromodeoxyuridine studies provide indirect evidence that mouse mammary adipocytes transform into secretory epithelial cells during pregnancy and revert to adipocytes after lactation. By using the Cre-loxP recombination system we show that the mammary gland of whey acidic protein (WAP)-Cre/R26R mice, in which secretory epithelial cells express the lacZ gene during pregnancy, contains labeled adipocytes during involution. Conversely, adipocyte P2-Cre/R26R mice, in which adipocytes are labeled before pregnancy, contain labeled secretory epithelial cells during pregnancy. We conclude that reversible adipocyte-to-epithelium and epithelium-to-adipocyte transdifferentiation occurs in the mammary gland of adult mice during pregnancy and lactation.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0407647101</identifier><identifier>PMID: 15556998</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Adipocytes ; Adipocytes - cytology ; Adipocytes - ultrastructure ; Animals ; Base Sequence ; Biological Sciences ; Breastfeeding & lactation ; Breasts ; Cell Differentiation ; Cellular biology ; DNA Primers ; Electron microscopy ; Epithelial cells ; Epithelial Cells - cytology ; Female ; Genes, Reporter ; Immunohistochemistry ; Lactation ; Lipids ; Mammary glands ; Mammary Glands, Animal - cytology ; Mammary Glands, Animal - ultrastructure ; Mice ; Mice, Transgenic ; Microscopy, Electron, Transmission ; Pregnancy ; Pulmonary alveoli ; Tissues ; White adipocytes</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2004-11, Vol.101 (48), p.16801-16806</ispartof><rights>Copyright 1993/2004 The National Academy of Sciences of the United States of America</rights><rights>Copyright National Academy of Sciences Nov 30, 2004</rights><rights>Copyright © 2004, The National Academy of Sciences 2004</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c528t-725b7c173545e1fedef33662dc1097e2f2373ed82b4d0b5cc15a17de106345a83</citedby><cites>FETCH-LOGICAL-c528t-725b7c173545e1fedef33662dc1097e2f2373ed82b4d0b5cc15a17de106345a83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/101/48.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/3373910$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/3373910$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,723,776,780,799,881,27901,27902,53766,53768,57992,58225</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15556998$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Morroni, Manrico</creatorcontrib><creatorcontrib>Giordano, Antonio</creatorcontrib><creatorcontrib>Zingaretti, Maria Cristina</creatorcontrib><creatorcontrib>Boiani, Romina</creatorcontrib><creatorcontrib>de Matteis, Rita</creatorcontrib><creatorcontrib>Kahn, Barbara B.</creatorcontrib><creatorcontrib>Nisoli, Enzo</creatorcontrib><creatorcontrib>Tonello, Cristina</creatorcontrib><creatorcontrib>Pisoschi, Catalina</creatorcontrib><creatorcontrib>Luchetti, Michele M.</creatorcontrib><creatorcontrib>Marelli, Mariella</creatorcontrib><creatorcontrib>Cinti, Saverio</creatorcontrib><creatorcontrib>Hay, Elizabeth D.</creatorcontrib><title>Reversible Transdifferentiation of Secretory Epithelial Cells into Adipocytes in the Mammary Gland</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Mammalian breast adipose tissue is replaced by a milk-secreting gland during pregnancy; the reverse process takes place upon interruption of lactation. Morphological and bromodeoxyuridine studies provide indirect evidence that mouse mammary adipocytes transform into secretory epithelial cells during pregnancy and revert to adipocytes after lactation. By using the Cre-loxP recombination system we show that the mammary gland of whey acidic protein (WAP)-Cre/R26R mice, in which secretory epithelial cells express the lacZ gene during pregnancy, contains labeled adipocytes during involution. Conversely, adipocyte P2-Cre/R26R mice, in which adipocytes are labeled before pregnancy, contain labeled secretory epithelial cells during pregnancy. 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the reverse process takes place upon interruption of lactation. Morphological and bromodeoxyuridine studies provide indirect evidence that mouse mammary adipocytes transform into secretory epithelial cells during pregnancy and revert to adipocytes after lactation. By using the Cre-loxP recombination system we show that the mammary gland of whey acidic protein (WAP)-Cre/R26R mice, in which secretory epithelial cells express the lacZ gene during pregnancy, contains labeled adipocytes during involution. Conversely, adipocyte P2-Cre/R26R mice, in which adipocytes are labeled before pregnancy, contain labeled secretory epithelial cells during pregnancy. We conclude that reversible adipocyte-to-epithelium and epithelium-to-adipocyte transdifferentiation occurs in the mammary gland of adult mice during pregnancy and lactation.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>15556998</pmid><doi>10.1073/pnas.0407647101</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| source | Jstor Complete Legacy; MEDLINE; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Adipocytes Adipocytes - cytology Adipocytes - ultrastructure Animals Base Sequence Biological Sciences Breastfeeding & lactation Breasts Cell Differentiation Cellular biology DNA Primers Electron microscopy Epithelial cells Epithelial Cells - cytology Female Genes, Reporter Immunohistochemistry Lactation Lipids Mammary glands Mammary Glands, Animal - cytology Mammary Glands, Animal - ultrastructure Mice Mice, Transgenic Microscopy, Electron, Transmission Pregnancy Pulmonary alveoli Tissues White adipocytes |
| title | Reversible Transdifferentiation of Secretory Epithelial Cells into Adipocytes in the Mammary Gland |
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