Viral Membrane Protein Topology Is Dictated by Multiple Determinants in Its Sequence

The targeting, insertion, and topology of membrane proteins have been extensively studied in both prokaryotes and eukaryotes. However, the mechanisms used by viral membrane proteins to generate the correct topology within cellular membranes are less well understood. Here, the effect of flanking char...

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Veröffentlicht in:Journal of molecular biology 2009-03, Vol.387 (1), p.113-128
Hauptverfasser: Saurí, Ana, Tamborero, Silvia, Martínez-Gil, Luis, Johnson, Arthur E., Mingarro, Ismael
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container_end_page 128
container_issue 1
container_start_page 113
container_title Journal of molecular biology
container_volume 387
creator Saurí, Ana
Tamborero, Silvia
Martínez-Gil, Luis
Johnson, Arthur E.
Mingarro, Ismael
description The targeting, insertion, and topology of membrane proteins have been extensively studied in both prokaryotes and eukaryotes. However, the mechanisms used by viral membrane proteins to generate the correct topology within cellular membranes are less well understood. Here, the effect of flanking charges and the hydrophobicity of the N-terminal hydrophobic segment on viral membrane protein topogenesis are examined systematically. Experimental data reveal that the classical topological determinants have only a minor effect on the overall topology of p9, a plant viral movement protein. Since only a few individual sequence alterations cause an inversion of p9 topology, its topological stability is robust. This result further indicates that the protein has multiple, and perhaps redundant, structural features that ensure that it always adopts the same topology. These critical topogenic sequences appear to be recognized and acted upon from the initial stages of protein biosynthesis, even before the ribosome ends protein translation.
doi_str_mv 10.1016/j.jmb.2009.01.063
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subjects Amino Acid Sequence
endoplasmic reticulum
Glycosylation
Membrane Proteins - chemistry
membrane topology
Molecular Sequence Data
Protein Conformation
translocon
transmembrane segment
viral protein
Viral Proteins - chemistry
title Viral Membrane Protein Topology Is Dictated by Multiple Determinants in Its Sequence
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