Phosphodiester hydrolysis and specific DNA binding and cleavage promoted by guanidinium-functionalized zinc complexes
Two new Zn(II) complexes containing guanidinium groups, [Zn(L 1)Cl 2](ClO 4) 2 · H 2O · CH 3OH ( 1) and [Zn(L 2)Cl 2](ClO 4) 2 · 0.5H 2O ( 2), were synthesized and characterized (L 1 = 5,5′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication and L 2 = 6,6′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication)....
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| Veröffentlicht in: | Journal of inorganic biochemistry 2009-05, Vol.103 (5), p.851-858 |
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| creator | He, Juan Sun, Jing Mao, Zong-Wan Ji, Liang-Nian Sun, Hongzhe |
| description | Two new Zn(II) complexes containing guanidinium groups, [Zn(L
1)Cl
2](ClO
4)
2
·
H
2O
·
CH
3OH (
1) and [Zn(L
2)Cl
2](ClO
4)
2
·
0.5H
2O (
2), were synthesized and characterized (L
1
=
5,5′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication and L
2
=
6,6′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication). Both complexes are able to catalyze bis(
p-nitrophenyl) phosphate (BNPP) hydrolysis efficiently. Obtained kinetic data reveal that both
1 and
2 show nearly 300- and 600-fold rate enhancement of BNPP hydrolysis, respectively, compared to their simple analogue without the guanidinium groups [Zn(bpy)Cl
2] (bpy
=
2,2′-bipyridy) (
3). Enhanced acceleration for cleavage of BNPP could be attributed to cooperative interaction between the Zn(II) ion and the guanidinium groups by electrostatic interaction and H-bonding. Studies on inhibition of sequence-specific endonucleases (
DraI and
SmaI) by complexes show that
1 and
2 are able to recognize nucleotide sequence, -TTT^AAA-, and highly effectively cleave the plasmid DNA in the presence of hydrogen peroxide, while
3 has no specific binding to the DNA target sequences and only shows low DNA cleavage activity. |
| doi_str_mv | 10.1016/j.jinorgbio.2009.02.010 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67138823</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0162013409000506</els_id><sourcerecordid>20224212</sourcerecordid><originalsourceid>FETCH-LOGICAL-c466t-c3cf71c664fa0413762408a69adc663dc6e68bfdd255da223fd909869830b8ad3</originalsourceid><addsrcrecordid>eNqFkUtv1DAQgC1ERZfCXwCfuCX4kTjJcVWeUtVygLPl2JPdWSV2sJOK7a-vy67g2Isteb7xPD5C3nNWcsbVx0N5QB_irsdQCsa6komScfaCbHjbyELKqnpJNpkUBeOyuiSvUzowxuq6al6RS95loKvlhqw_9iHN--AQ0gKR7o8uhvGYMFHjHU0zWBzQ0k-3W9qjd-h3fwN2BHNvdkDnGKawgKP9ke5W4zEjuE7FsHq7YPBmxIccfUBvqQ3TPMIfSG_IxWDGBG_P9xX59eXzz-tvxc3d1-_X25vCVkothZV2aLhVqhoMq7hslKhYa1RnXH6U-QDV9oNzoq6dEUIOrmNdq7pWsr41Tl6RD6d_c5e_1zyhnjBZGEfjIaxJq4bLthXyWVAwISrBRQabE2hjSCnCoOeIk4lHzZl-UqMP-p8a_aRGM6Gzmpz57lxi7Sdw__POLjKwPQGQN3KPEHWyCN6Cwwh20S7gs0UeAVUjprM</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20224212</pqid></control><display><type>article</type><title>Phosphodiester hydrolysis and specific DNA binding and cleavage promoted by guanidinium-functionalized zinc complexes</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>He, Juan ; Sun, Jing ; Mao, Zong-Wan ; Ji, Liang-Nian ; Sun, Hongzhe</creator><creatorcontrib>He, Juan ; Sun, Jing ; Mao, Zong-Wan ; Ji, Liang-Nian ; Sun, Hongzhe</creatorcontrib><description>Two new Zn(II) complexes containing guanidinium groups, [Zn(L
1)Cl
2](ClO
4)
2
·
H
2O
·
CH
3OH (
1) and [Zn(L
2)Cl
2](ClO
4)
2
·
0.5H
2O (
2), were synthesized and characterized (L
1
=
5,5′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication and L
2
=
6,6′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication). Both complexes are able to catalyze bis(
p-nitrophenyl) phosphate (BNPP) hydrolysis efficiently. Obtained kinetic data reveal that both
1 and
2 show nearly 300- and 600-fold rate enhancement of BNPP hydrolysis, respectively, compared to their simple analogue without the guanidinium groups [Zn(bpy)Cl
2] (bpy
=
2,2′-bipyridy) (
3). Enhanced acceleration for cleavage of BNPP could be attributed to cooperative interaction between the Zn(II) ion and the guanidinium groups by electrostatic interaction and H-bonding. Studies on inhibition of sequence-specific endonucleases (
DraI and
SmaI) by complexes show that
1 and
2 are able to recognize nucleotide sequence, -TTT^AAA-, and highly effectively cleave the plasmid DNA in the presence of hydrogen peroxide, while
3 has no specific binding to the DNA target sequences and only shows low DNA cleavage activity.</description><identifier>ISSN: 0162-0134</identifier><identifier>EISSN: 1873-3344</identifier><identifier>DOI: 10.1016/j.jinorgbio.2009.02.010</identifier><identifier>PMID: 19344953</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>DNA - chemistry ; DNA Cleavage ; Guanidine - chemistry ; Guanidinium ; Hydrolysis ; Inhibition ; Kinetics ; Models, Molecular ; Molecular Structure ; Organophosphates - chemistry ; Phosphate diester ; Zinc Compounds - chemistry</subject><ispartof>Journal of inorganic biochemistry, 2009-05, Vol.103 (5), p.851-858</ispartof><rights>2009 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c466t-c3cf71c664fa0413762408a69adc663dc6e68bfdd255da223fd909869830b8ad3</citedby><cites>FETCH-LOGICAL-c466t-c3cf71c664fa0413762408a69adc663dc6e68bfdd255da223fd909869830b8ad3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0162013409000506$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19344953$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>He, Juan</creatorcontrib><creatorcontrib>Sun, Jing</creatorcontrib><creatorcontrib>Mao, Zong-Wan</creatorcontrib><creatorcontrib>Ji, Liang-Nian</creatorcontrib><creatorcontrib>Sun, Hongzhe</creatorcontrib><title>Phosphodiester hydrolysis and specific DNA binding and cleavage promoted by guanidinium-functionalized zinc complexes</title><title>Journal of inorganic biochemistry</title><addtitle>J Inorg Biochem</addtitle><description>Two new Zn(II) complexes containing guanidinium groups, [Zn(L
1)Cl
2](ClO
4)
2
·
H
2O
·
CH
3OH (
1) and [Zn(L
2)Cl
2](ClO
4)
2
·
0.5H
2O (
2), were synthesized and characterized (L
1
=
5,5′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication and L
2
=
6,6′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication). Both complexes are able to catalyze bis(
p-nitrophenyl) phosphate (BNPP) hydrolysis efficiently. Obtained kinetic data reveal that both
1 and
2 show nearly 300- and 600-fold rate enhancement of BNPP hydrolysis, respectively, compared to their simple analogue without the guanidinium groups [Zn(bpy)Cl
2] (bpy
=
2,2′-bipyridy) (
3). Enhanced acceleration for cleavage of BNPP could be attributed to cooperative interaction between the Zn(II) ion and the guanidinium groups by electrostatic interaction and H-bonding. Studies on inhibition of sequence-specific endonucleases (
DraI and
SmaI) by complexes show that
1 and
2 are able to recognize nucleotide sequence, -TTT^AAA-, and highly effectively cleave the plasmid DNA in the presence of hydrogen peroxide, while
3 has no specific binding to the DNA target sequences and only shows low DNA cleavage activity.</description><subject>DNA - chemistry</subject><subject>DNA Cleavage</subject><subject>Guanidine - chemistry</subject><subject>Guanidinium</subject><subject>Hydrolysis</subject><subject>Inhibition</subject><subject>Kinetics</subject><subject>Models, Molecular</subject><subject>Molecular Structure</subject><subject>Organophosphates - chemistry</subject><subject>Phosphate diester</subject><subject>Zinc Compounds - chemistry</subject><issn>0162-0134</issn><issn>1873-3344</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAQgC1ERZfCXwCfuCX4kTjJcVWeUtVygLPl2JPdWSV2sJOK7a-vy67g2Isteb7xPD5C3nNWcsbVx0N5QB_irsdQCsa6komScfaCbHjbyELKqnpJNpkUBeOyuiSvUzowxuq6al6RS95loKvlhqw_9iHN--AQ0gKR7o8uhvGYMFHjHU0zWBzQ0k-3W9qjd-h3fwN2BHNvdkDnGKawgKP9ke5W4zEjuE7FsHq7YPBmxIccfUBvqQ3TPMIfSG_IxWDGBG_P9xX59eXzz-tvxc3d1-_X25vCVkothZV2aLhVqhoMq7hslKhYa1RnXH6U-QDV9oNzoq6dEUIOrmNdq7pWsr41Tl6RD6d_c5e_1zyhnjBZGEfjIaxJq4bLthXyWVAwISrBRQabE2hjSCnCoOeIk4lHzZl-UqMP-p8a_aRGM6Gzmpz57lxi7Sdw__POLjKwPQGQN3KPEHWyCN6Cwwh20S7gs0UeAVUjprM</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>He, Juan</creator><creator>Sun, Jing</creator><creator>Mao, Zong-Wan</creator><creator>Ji, Liang-Nian</creator><creator>Sun, Hongzhe</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>F1W</scope><scope>H96</scope><scope>L.G</scope><scope>7X8</scope></search><sort><creationdate>20090501</creationdate><title>Phosphodiester hydrolysis and specific DNA binding and cleavage promoted by guanidinium-functionalized zinc complexes</title><author>He, Juan ; Sun, Jing ; Mao, Zong-Wan ; Ji, Liang-Nian ; Sun, Hongzhe</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c466t-c3cf71c664fa0413762408a69adc663dc6e68bfdd255da223fd909869830b8ad3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>DNA - chemistry</topic><topic>DNA Cleavage</topic><topic>Guanidine - chemistry</topic><topic>Guanidinium</topic><topic>Hydrolysis</topic><topic>Inhibition</topic><topic>Kinetics</topic><topic>Models, Molecular</topic><topic>Molecular Structure</topic><topic>Organophosphates - chemistry</topic><topic>Phosphate diester</topic><topic>Zinc Compounds - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>He, Juan</creatorcontrib><creatorcontrib>Sun, Jing</creatorcontrib><creatorcontrib>Mao, Zong-Wan</creatorcontrib><creatorcontrib>Ji, Liang-Nian</creatorcontrib><creatorcontrib>Sun, Hongzhe</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 2: Ocean Technology, Policy & Non-Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of inorganic biochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>He, Juan</au><au>Sun, Jing</au><au>Mao, Zong-Wan</au><au>Ji, Liang-Nian</au><au>Sun, Hongzhe</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Phosphodiester hydrolysis and specific DNA binding and cleavage promoted by guanidinium-functionalized zinc complexes</atitle><jtitle>Journal of inorganic biochemistry</jtitle><addtitle>J Inorg Biochem</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>103</volume><issue>5</issue><spage>851</spage><epage>858</epage><pages>851-858</pages><issn>0162-0134</issn><eissn>1873-3344</eissn><abstract>Two new Zn(II) complexes containing guanidinium groups, [Zn(L
1)Cl
2](ClO
4)
2
·
H
2O
·
CH
3OH (
1) and [Zn(L
2)Cl
2](ClO
4)
2
·
0.5H
2O (
2), were synthesized and characterized (L
1
=
5,5′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication and L
2
=
6,6′-di[1-(guanidyl)methyl]-2,2′-bipyridyl bication). Both complexes are able to catalyze bis(
p-nitrophenyl) phosphate (BNPP) hydrolysis efficiently. Obtained kinetic data reveal that both
1 and
2 show nearly 300- and 600-fold rate enhancement of BNPP hydrolysis, respectively, compared to their simple analogue without the guanidinium groups [Zn(bpy)Cl
2] (bpy
=
2,2′-bipyridy) (
3). Enhanced acceleration for cleavage of BNPP could be attributed to cooperative interaction between the Zn(II) ion and the guanidinium groups by electrostatic interaction and H-bonding. Studies on inhibition of sequence-specific endonucleases (
DraI and
SmaI) by complexes show that
1 and
2 are able to recognize nucleotide sequence, -TTT^AAA-, and highly effectively cleave the plasmid DNA in the presence of hydrogen peroxide, while
3 has no specific binding to the DNA target sequences and only shows low DNA cleavage activity.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>19344953</pmid><doi>10.1016/j.jinorgbio.2009.02.010</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0162-0134 |
| ispartof | Journal of inorganic biochemistry, 2009-05, Vol.103 (5), p.851-858 |
| issn | 0162-0134 1873-3344 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67138823 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | DNA - chemistry DNA Cleavage Guanidine - chemistry Guanidinium Hydrolysis Inhibition Kinetics Models, Molecular Molecular Structure Organophosphates - chemistry Phosphate diester Zinc Compounds - chemistry |
| title | Phosphodiester hydrolysis and specific DNA binding and cleavage promoted by guanidinium-functionalized zinc complexes |
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