Glutamate Is the Major Anaplerotic Substrate in the Tricarboxylic Acid Cycle of Isolated Rumen Epithelial and Duodenal Mucosal Cells from Beef Cattle

In this study, we aimed to determine the contribution of substrates to tricarboxylic acid (TCA) cycle fluxes in rumen epithelial cells (REC) and duodenal mucosal cells (DMC) isolated from Angus bulls (n = 6) fed either a 75% forage (HF) or 75% concentrate (HC) diet. In separate incubations, [¹³C₆]gl...

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Veröffentlicht in:	The Journal of nutrition 2009-05, Vol.139 (5), p.869-875
Hauptverfasser:	El-Kadi, Samer W, Baldwin, Ransom L. VI, McLeod, Kyle R, Sunny, Nishanth E, Bequette, Brian J
Format:	Artikel
Sprache:	eng
Schlagworte:	amino acid metabolism Animals beef cattle Biological and medical sciences Carbon Isotopes Cattle - metabolism Citric Acid Cycle Diet Duodenum epithelial cells Epithelial Cells - metabolism Feeding. Feeding behavior Fundamental and applied biological sciences. Psychology Gas Chromatography-Mass Spectrometry Glucose - metabolism glutamic acid Glutamic Acid - metabolism Glutamine - metabolism intestinal mucosa Intestinal Mucosa - cytology Lactic Acid - metabolism Leucine - metabolism Male nutrition physiology Pyruvic Acid - metabolism rumen Rumen - cytology ruminant nutrition tricarboxylic acid cycle Valine - metabolism Vertebrates: anatomy and physiology, studies on body, several organs or systems
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