Transgenic resistance by N gene of a Peanut bud necrosis virus isolate of characteristic phylogeny
The nucleocapsid protein ( N ) gene of a Tospovirus devastating tomato crop in the south Indian state of Tamil Nadu was cloned and characterized. The high identity of the cloned sequence to a Peanut bud necrosis virus (PBNV) tomato isolate (97.8/99.6% nucleotide/amino acid) and a PBNV peanut isolate...
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| Veröffentlicht in: | Virus genes 2009-06, Vol.38 (3), p.445-454 |
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| creator | Venkatesan, S. Raja, J. A. J. Maruthasalam, S. Kumar, K. K. Ramanathan, A. Sudhakar, D. Balasubramanian, P. |
| description | The nucleocapsid protein (
N
) gene of a Tospovirus devastating tomato crop in the south Indian state of Tamil Nadu was cloned and characterized. The high identity of the cloned sequence to a
Peanut bud necrosis virus
(PBNV) tomato isolate (97.8/99.6% nucleotide/amino acid) and a PBNV peanut isolate (94.4/96.3% nucleotide/amino acid) identified the Tospovirus as an isolate of PBNV, designated PBNV Coimbatore tomato (PBNV CT) isolate. Phylogenetic analysis of
PBNV CT N
gene provided useful insights into the movement and evolution of PBNV within Indian Territory. The characteristic phylogeny of
PBNV CT N
gene implied its potential to be an efficient transgene to confer effective PBNV resistance on crop plants. The efficacy of
PBNV CT N
gene in conferring PBNV resistance was studied by generating tobacco (
Nicotiana tabacum
L. cv Wisconsin) lines transgenic to the sense or antisense version of the gene. Several transgenic lines showed transgenic mRNA and/or protein accumulation, ranging from very high to undetectable levels, accompanied by different degrees of PBNV resistance. The undetectable or very low levels of transgene transcripts in certain PBNV-resistant sense or antisense
N
gene transgenic lines suggested RNA-mediated resistance by post-transcriptional gene silencing (PTGS) mechanism. However, PBNV resistance of certain transgenic lines with high levels of
N
gene transcripts was suggestive of possible operation of RNA-mediated non-PTGS mechanism(s) of resistance in those lines. Moreover, the high levels of N protein in certain PBNV-resistant sense
N
gene transgenic lines suggested protein-mediated resistance. The results predict the potential of
PBNV CT N
gene to confer effective PBNV resistance on tomato and other economically important crops. |
| doi_str_mv | 10.1007/s11262-009-0342-5 |
| format | Article |
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N
) gene of a Tospovirus devastating tomato crop in the south Indian state of Tamil Nadu was cloned and characterized. The high identity of the cloned sequence to a
Peanut bud necrosis virus
(PBNV) tomato isolate (97.8/99.6% nucleotide/amino acid) and a PBNV peanut isolate (94.4/96.3% nucleotide/amino acid) identified the Tospovirus as an isolate of PBNV, designated PBNV Coimbatore tomato (PBNV CT) isolate. Phylogenetic analysis of
PBNV CT N
gene provided useful insights into the movement and evolution of PBNV within Indian Territory. The characteristic phylogeny of
PBNV CT N
gene implied its potential to be an efficient transgene to confer effective PBNV resistance on crop plants. The efficacy of
PBNV CT N
gene in conferring PBNV resistance was studied by generating tobacco (
Nicotiana tabacum
L. cv Wisconsin) lines transgenic to the sense or antisense version of the gene. Several transgenic lines showed transgenic mRNA and/or protein accumulation, ranging from very high to undetectable levels, accompanied by different degrees of PBNV resistance. The undetectable or very low levels of transgene transcripts in certain PBNV-resistant sense or antisense
N
gene transgenic lines suggested RNA-mediated resistance by post-transcriptional gene silencing (PTGS) mechanism. However, PBNV resistance of certain transgenic lines with high levels of
N
gene transcripts was suggestive of possible operation of RNA-mediated non-PTGS mechanism(s) of resistance in those lines. Moreover, the high levels of N protein in certain PBNV-resistant sense
N
gene transgenic lines suggested protein-mediated resistance. The results predict the potential of
PBNV CT N
gene to confer effective PBNV resistance on tomato and other economically important crops.</description><identifier>ISSN: 0920-8569</identifier><identifier>EISSN: 1572-994X</identifier><identifier>DOI: 10.1007/s11262-009-0342-5</identifier><identifier>PMID: 19255836</identifier><language>eng</language><publisher>New York: Springer US</publisher><subject>Arachis hypogaea ; Biomedical and Life Sciences ; Biomedicine ; Immunity, Innate ; Lycopersicon esculentum ; Medical Microbiology ; Nicotiana - genetics ; Nicotiana tabacum ; Nucleocapsid Proteins - biosynthesis ; Nucleocapsid Proteins - genetics ; Phylogeny ; Plant Diseases - immunology ; Plant Diseases - virology ; Plant Sciences ; Plants, Genetically Modified - genetics ; Plants, Genetically Modified - immunology ; Plants, Genetically Modified - virology ; RNA Interference ; RNA, Viral - genetics ; Sequence Analysis, DNA ; Sequence Homology ; Solanum lycopersicum - virology ; Tospovirus ; Tospovirus - genetics ; Tospovirus - isolation & purification ; Virology ; Wisconsin</subject><ispartof>Virus genes, 2009-06, Vol.38 (3), p.445-454</ispartof><rights>Springer Science+Business Media, LLC 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c400t-94c9bd813dfc13e6775c8ed683ed1a767dbd8fb3f63f0e4e81d5707f9224f8183</citedby><cites>FETCH-LOGICAL-c400t-94c9bd813dfc13e6775c8ed683ed1a767dbd8fb3f63f0e4e81d5707f9224f8183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11262-009-0342-5$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11262-009-0342-5$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,780,784,27924,27925,41488,42557,51319</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19255836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Venkatesan, S.</creatorcontrib><creatorcontrib>Raja, J. A. J.</creatorcontrib><creatorcontrib>Maruthasalam, S.</creatorcontrib><creatorcontrib>Kumar, K. K.</creatorcontrib><creatorcontrib>Ramanathan, A.</creatorcontrib><creatorcontrib>Sudhakar, D.</creatorcontrib><creatorcontrib>Balasubramanian, P.</creatorcontrib><title>Transgenic resistance by N gene of a Peanut bud necrosis virus isolate of characteristic phylogeny</title><title>Virus genes</title><addtitle>Virus Genes</addtitle><addtitle>Virus Genes</addtitle><description>The nucleocapsid protein (
N
) gene of a Tospovirus devastating tomato crop in the south Indian state of Tamil Nadu was cloned and characterized. The high identity of the cloned sequence to a
Peanut bud necrosis virus
(PBNV) tomato isolate (97.8/99.6% nucleotide/amino acid) and a PBNV peanut isolate (94.4/96.3% nucleotide/amino acid) identified the Tospovirus as an isolate of PBNV, designated PBNV Coimbatore tomato (PBNV CT) isolate. Phylogenetic analysis of
PBNV CT N
gene provided useful insights into the movement and evolution of PBNV within Indian Territory. The characteristic phylogeny of
PBNV CT N
gene implied its potential to be an efficient transgene to confer effective PBNV resistance on crop plants. The efficacy of
PBNV CT N
gene in conferring PBNV resistance was studied by generating tobacco (
Nicotiana tabacum
L. cv Wisconsin) lines transgenic to the sense or antisense version of the gene. Several transgenic lines showed transgenic mRNA and/or protein accumulation, ranging from very high to undetectable levels, accompanied by different degrees of PBNV resistance. The undetectable or very low levels of transgene transcripts in certain PBNV-resistant sense or antisense
N
gene transgenic lines suggested RNA-mediated resistance by post-transcriptional gene silencing (PTGS) mechanism. However, PBNV resistance of certain transgenic lines with high levels of
N
gene transcripts was suggestive of possible operation of RNA-mediated non-PTGS mechanism(s) of resistance in those lines. Moreover, the high levels of N protein in certain PBNV-resistant sense
N
gene transgenic lines suggested protein-mediated resistance. The results predict the potential of
PBNV CT N
gene to confer effective PBNV resistance on tomato and other economically important crops.</description><subject>Arachis hypogaea</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Immunity, Innate</subject><subject>Lycopersicon esculentum</subject><subject>Medical Microbiology</subject><subject>Nicotiana - genetics</subject><subject>Nicotiana tabacum</subject><subject>Nucleocapsid Proteins - biosynthesis</subject><subject>Nucleocapsid Proteins - genetics</subject><subject>Phylogeny</subject><subject>Plant Diseases - immunology</subject><subject>Plant Diseases - virology</subject><subject>Plant Sciences</subject><subject>Plants, Genetically Modified - genetics</subject><subject>Plants, Genetically Modified - immunology</subject><subject>Plants, Genetically Modified - virology</subject><subject>RNA Interference</subject><subject>RNA, Viral - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology</subject><subject>Solanum lycopersicum - virology</subject><subject>Tospovirus</subject><subject>Tospovirus - genetics</subject><subject>Tospovirus - isolation & purification</subject><subject>Virology</subject><subject>Wisconsin</subject><issn>0920-8569</issn><issn>1572-994X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqNkV1LHDEUhkNR6mr7A3pTQi96N_UkmXxdFrFakOqFgnchkznZHZmdWZOZwv77Zt0FQSh4Fch5zhPyvoR8YfCDAejzzBhXvAKwFYiaV_IDWTCpeWVt_XhEFmA5VEYqe0JOc34CAGN4_ZGcMMulNEItSHOf_JCXOHSBJsxdnvwQkDZb-oeWW6RjpJ7eoR_miTZzSwcMaSwc_dulOdMuj72fXrCw8smHCVORFNtmte3Hoth-IsfR9xk_H84z8vDr8v7iurq5vfp98fOmCjXAVNk62KY1TLQxMIFKaxkMtsoIbJnXSrdlGhsRlYiANRrWSg06Ws7raJgRZ-T73rtJ4_OMeXLrLgfsez_gOGenNONGvgPkjJeALBTw2xvwaZzTUD7hOBdKlRBFgdge2uWSE0a3Sd3ap61j4HY1uX1NrtTkdjU5WXa-HsRzs8b2dePQSwH4HshlNCwxvb78f-s_eJ6dfg</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Venkatesan, S.</creator><creator>Raja, J. A. J.</creator><creator>Maruthasalam, S.</creator><creator>Kumar, K. K.</creator><creator>Ramanathan, A.</creator><creator>Sudhakar, D.</creator><creator>Balasubramanian, P.</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TM</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>20090601</creationdate><title>Transgenic resistance by N gene of a Peanut bud necrosis virus isolate of characteristic phylogeny</title><author>Venkatesan, S. ; Raja, J. 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A. J.</creatorcontrib><creatorcontrib>Maruthasalam, S.</creatorcontrib><creatorcontrib>Kumar, K. 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A. J.</au><au>Maruthasalam, S.</au><au>Kumar, K. K.</au><au>Ramanathan, A.</au><au>Sudhakar, D.</au><au>Balasubramanian, P.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transgenic resistance by N gene of a Peanut bud necrosis virus isolate of characteristic phylogeny</atitle><jtitle>Virus genes</jtitle><stitle>Virus Genes</stitle><addtitle>Virus Genes</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>38</volume><issue>3</issue><spage>445</spage><epage>454</epage><pages>445-454</pages><issn>0920-8569</issn><eissn>1572-994X</eissn><abstract>The nucleocapsid protein (
N
) gene of a Tospovirus devastating tomato crop in the south Indian state of Tamil Nadu was cloned and characterized. The high identity of the cloned sequence to a
Peanut bud necrosis virus
(PBNV) tomato isolate (97.8/99.6% nucleotide/amino acid) and a PBNV peanut isolate (94.4/96.3% nucleotide/amino acid) identified the Tospovirus as an isolate of PBNV, designated PBNV Coimbatore tomato (PBNV CT) isolate. Phylogenetic analysis of
PBNV CT N
gene provided useful insights into the movement and evolution of PBNV within Indian Territory. The characteristic phylogeny of
PBNV CT N
gene implied its potential to be an efficient transgene to confer effective PBNV resistance on crop plants. The efficacy of
PBNV CT N
gene in conferring PBNV resistance was studied by generating tobacco (
Nicotiana tabacum
L. cv Wisconsin) lines transgenic to the sense or antisense version of the gene. Several transgenic lines showed transgenic mRNA and/or protein accumulation, ranging from very high to undetectable levels, accompanied by different degrees of PBNV resistance. The undetectable or very low levels of transgene transcripts in certain PBNV-resistant sense or antisense
N
gene transgenic lines suggested RNA-mediated resistance by post-transcriptional gene silencing (PTGS) mechanism. However, PBNV resistance of certain transgenic lines with high levels of
N
gene transcripts was suggestive of possible operation of RNA-mediated non-PTGS mechanism(s) of resistance in those lines. Moreover, the high levels of N protein in certain PBNV-resistant sense
N
gene transgenic lines suggested protein-mediated resistance. The results predict the potential of
PBNV CT N
gene to confer effective PBNV resistance on tomato and other economically important crops.</abstract><cop>New York</cop><pub>Springer US</pub><pmid>19255836</pmid><doi>10.1007/s11262-009-0342-5</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Virus genes, 2009-06, Vol.38 (3), p.445-454 |
| issn | 0920-8569 1572-994X |
| language | eng |
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| source | MEDLINE; SpringerNature Journals |
| subjects | Arachis hypogaea Biomedical and Life Sciences Biomedicine Immunity, Innate Lycopersicon esculentum Medical Microbiology Nicotiana - genetics Nicotiana tabacum Nucleocapsid Proteins - biosynthesis Nucleocapsid Proteins - genetics Phylogeny Plant Diseases - immunology Plant Diseases - virology Plant Sciences Plants, Genetically Modified - genetics Plants, Genetically Modified - immunology Plants, Genetically Modified - virology RNA Interference RNA, Viral - genetics Sequence Analysis, DNA Sequence Homology Solanum lycopersicum - virology Tospovirus Tospovirus - genetics Tospovirus - isolation & purification Virology Wisconsin |
| title | Transgenic resistance by N gene of a Peanut bud necrosis virus isolate of characteristic phylogeny |
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