Effects of Aspirin on Clot Structure and Fibrinolysis Using a Novel In Vitro Cellular System

The purpose of this study was to investigate the direct effects of aspirin on fibrin structure/function. Chinese Hamster Ovary cell lines stably transfected with fibrinogen were grown in the absence (0) and presence of increasing concentrations of aspirin. Fibrinogen was purified from the media usin...

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Veröffentlicht in:	Arteriosclerosis, thrombosis, and vascular biology thrombosis, and vascular biology, 2009-05, Vol.29 (5), p.712-717
Hauptverfasser:	AJJAN, R. A, STANDEVEN, K. F, KHANBHAI, M, PHOENIX, F, GERSH, K. C, WEISEL, J. W, KEARNEY, M. T, ARIËNS, R. A. S, GRANT, P. J
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Aspirin - pharmacology Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Blood Coagulation - drug effects Cardiology. Vascular system Cardiovascular system CHO Cells Cricetinae Cricetulus Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Fibrinogen - drug effects Fibrinolysis - drug effects Medical sciences Pharmacology. Drug treatments Vasodilator agents. Cerebral vasodilators
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Zusammenfassung:	The purpose of this study was to investigate the direct effects of aspirin on fibrin structure/function. Chinese Hamster Ovary cell lines stably transfected with fibrinogen were grown in the absence (0) and presence of increasing concentrations of aspirin. Fibrinogen was purified from the media using affinity chromatography, and clots were made from recombinant protein. Mean final turbidity [OD(+/-SEM)] was 0.083(+/-0.03), 0.093(+/-0.002), 0.101(+/-0.005), and 0.125(+/-0.003) in clots made from 0, 1, 10, and 100 mg/L aspirin-treated fibrinogen, respectively (P
ISSN:	1079-5642 1524-4636
DOI:	10.1161/ATVBAHA.109.183707