Characterization of fluoroquinolone-resistant Shigella flexneri in Hangzhou area of China
Objectives The aim of this study was to characterize fluoroquinolone-resistant Shigella and determine whether the qnr and aac(6′)-Ib-cr genes could contribute to sporadic shigellosis at the clinic in the Hangzhou area of China. Methods A total of 202 strains of Shigella (79 Shigella sonnei and 123 S...
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| creator | Pu, Xiao-Ying Pan, Jing-Cao Wang, Hao-Qiu Zhang, Wei Huang, Zhi-Cheng Gu, Yang-Ming |
| description | Objectives The aim of this study was to characterize fluoroquinolone-resistant Shigella and determine whether the qnr and aac(6′)-Ib-cr genes could contribute to sporadic shigellosis at the clinic in the Hangzhou area of China. Methods A total of 202 strains of Shigella (79 Shigella sonnei and 123 Shigella flexneri ) isolated from sporadic cases of shigellosis from 1998 to 2007 were analysed for their antimicrobial susceptibility. The gyrA, gyrB, parC, parE, qnr and aac(6′)-Ib-cr genes and the profiles and incompatibility of plasmids were characterized. Chromosomal DNA fingerprinting was determined by XbaI-based digestion and PFGE. Results All strains of S. sonnei were susceptible to fluoroquinolones (ciprofloxacin and levofloxacin) while 15 out of 123 strains of S. flexneri were resistant. All of the 15 resistant strains displayed common mutations in the gyrA and parC genes and formed eight distinct groups with unique molecular characteristics. Notably, 10 isolates showed mutations at codon 87 of gyrA, and the other 5 were qnrS-positive. Two strains were positive for the aac(6′)-Ib-cr gene. Importantly, this is the first report of qnrS- and aac(6′)-Ib-cr-positive Shigella in China, the qnrS-positive S. flexneri serotypes 1a, 2a and 4c and the aac(6’)-Ib-cr-positive S. flexneri serotypes 2a and 4c worldwide. Conclusions The common mutations at position 83 of gyrA and position 80 of parC were crucial for resistance to nalidixic acid in S. flexneri. The mutation at position 87 of gyrA or the presence of the qnrS gene is necessary for high-level resistance to fluoroquinolones in Shigella isolates from China. |
| doi_str_mv | 10.1093/jac/dkp087 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67116436</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><oup_id>10.1093/jac/dkp087</oup_id><sourcerecordid>67116436</sourcerecordid><originalsourceid>FETCH-LOGICAL-c543t-2a756e40245120a7a43f4cfa83c2c4c99ece827903e50a7696db7d8d7b503b2b3</originalsourceid><addsrcrecordid>eNqF0c9rFDEUB_Agil2rF_8AGQQ9CGPzO5NjXawr1ApWQb2EN5lMN9vZZE1moPavN8MuLXjQUw75vJf38kXoOcFvCdbsZAP2pLve4UY9QAvCJa4p1uQhWmCGRa24YEfoSc4bjLEUsnmMjoimWjHVLNCP5RoS2NElfwujj6GKfdUPU0zx1-RDHGJwdXLZ5xHCWF2u_ZUbBijE3YRSVPlQrSBc3a7jVEFyMNcv1z7AU_SohyG7Z4fzGH07e_91uarPP3_4uDw9r63gbKwpKCEdx5QLQjEo4KzntoeGWWq51dpZ11ClMXOiXEstu1Z1TadagVlLW3aMXu_77uaRXR7N1mc7DxlcnLKRihDJmfwvpFhoKqgq8OVfcBOnFMoShhIlJcdkRm_2yKaYc3K92SW_hfTbEGzmWEyJxexjKfjFoePUbl13Tw85FPDqACBbGPoEwfp85yjhiuFG37s47f79YL13JTd3cychXZf_YEqY1fef5p38pM6-XFyYS_YHOiqxnA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>217664017</pqid></control><display><type>article</type><title>Characterization of fluoroquinolone-resistant Shigella flexneri in Hangzhou area of China</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Oxford University Press Journals All Titles (1996-Current)</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Pu, Xiao-Ying ; Pan, Jing-Cao ; Wang, Hao-Qiu ; Zhang, Wei ; Huang, Zhi-Cheng ; Gu, Yang-Ming</creator><creatorcontrib>Pu, Xiao-Ying ; Pan, Jing-Cao ; Wang, Hao-Qiu ; Zhang, Wei ; Huang, Zhi-Cheng ; Gu, Yang-Ming</creatorcontrib><description>Objectives The aim of this study was to characterize fluoroquinolone-resistant Shigella and determine whether the qnr and aac(6′)-Ib-cr genes could contribute to sporadic shigellosis at the clinic in the Hangzhou area of China. Methods A total of 202 strains of Shigella (79 Shigella sonnei and 123 Shigella flexneri ) isolated from sporadic cases of shigellosis from 1998 to 2007 were analysed for their antimicrobial susceptibility. The gyrA, gyrB, parC, parE, qnr and aac(6′)-Ib-cr genes and the profiles and incompatibility of plasmids were characterized. Chromosomal DNA fingerprinting was determined by XbaI-based digestion and PFGE. Results All strains of S. sonnei were susceptible to fluoroquinolones (ciprofloxacin and levofloxacin) while 15 out of 123 strains of S. flexneri were resistant. All of the 15 resistant strains displayed common mutations in the gyrA and parC genes and formed eight distinct groups with unique molecular characteristics. Notably, 10 isolates showed mutations at codon 87 of gyrA, and the other 5 were qnrS-positive. Two strains were positive for the aac(6′)-Ib-cr gene. Importantly, this is the first report of qnrS- and aac(6′)-Ib-cr-positive Shigella in China, the qnrS-positive S. flexneri serotypes 1a, 2a and 4c and the aac(6’)-Ib-cr-positive S. flexneri serotypes 2a and 4c worldwide. Conclusions The common mutations at position 83 of gyrA and position 80 of parC were crucial for resistance to nalidixic acid in S. flexneri. The mutation at position 87 of gyrA or the presence of the qnrS gene is necessary for high-level resistance to fluoroquinolones in Shigella isolates from China.</description><identifier>ISSN: 0305-7453</identifier><identifier>EISSN: 1460-2091</identifier><identifier>DOI: 10.1093/jac/dkp087</identifier><identifier>PMID: 19297378</identifier><identifier>CODEN: JACHDX</identifier><language>eng</language><publisher>Oxford: Oxford University Press</publisher><subject>aac(6′)-Ib-cr ; Anti-Bacterial Agents - pharmacology ; antibiotic resistance ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Bacteria ; Bacterial Proteins - genetics ; Bacterial Typing Techniques ; Biological and medical sciences ; China ; Cluster Analysis ; Deoxyribonucleic acid ; DNA ; DNA Fingerprinting ; DNA Gyrase - genetics ; DNA Topoisomerase IV - genetics ; Drug resistance ; Drug Resistance, Bacterial ; Dysentery, Bacillary - microbiology ; Electrophoresis, Gel, Pulsed-Field ; Fluoroquinolones - pharmacology ; Genetics ; Genotype ; Humans ; Medical sciences ; Microbial Sensitivity Tests ; Mutation ; Mutation, Missense ; Pharmacology. Drug treatments ; plasmid replicon ; Plasmids ; qnrS ; Shigella flexneri ; Shigella flexneri - drug effects ; Shigella flexneri - isolation & purification ; Shigella sonnei ; Shigella sonnei - drug effects ; Shigella sonnei - isolation & purification</subject><ispartof>Journal of antimicrobial chemotherapy, 2009-05, Vol.63 (5), p.917-920</ispartof><rights>The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org 2009</rights><rights>2009 INIST-CNRS</rights><rights>The Author 2009. Published by Oxford University Press on behalf of the British Society for Antimicrobial Chemotherapy. All rights reserved. For Permissions, please e-mail: journals.permissions@oxfordjournals.org</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c543t-2a756e40245120a7a43f4cfa83c2c4c99ece827903e50a7696db7d8d7b503b2b3</citedby><cites>FETCH-LOGICAL-c543t-2a756e40245120a7a43f4cfa83c2c4c99ece827903e50a7696db7d8d7b503b2b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,1579,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21473089$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19297378$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pu, Xiao-Ying</creatorcontrib><creatorcontrib>Pan, Jing-Cao</creatorcontrib><creatorcontrib>Wang, Hao-Qiu</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Huang, Zhi-Cheng</creatorcontrib><creatorcontrib>Gu, Yang-Ming</creatorcontrib><title>Characterization of fluoroquinolone-resistant Shigella flexneri in Hangzhou area of China</title><title>Journal of antimicrobial chemotherapy</title><addtitle>J Antimicrob Chemother</addtitle><description>Objectives The aim of this study was to characterize fluoroquinolone-resistant Shigella and determine whether the qnr and aac(6′)-Ib-cr genes could contribute to sporadic shigellosis at the clinic in the Hangzhou area of China. Methods A total of 202 strains of Shigella (79 Shigella sonnei and 123 Shigella flexneri ) isolated from sporadic cases of shigellosis from 1998 to 2007 were analysed for their antimicrobial susceptibility. The gyrA, gyrB, parC, parE, qnr and aac(6′)-Ib-cr genes and the profiles and incompatibility of plasmids were characterized. Chromosomal DNA fingerprinting was determined by XbaI-based digestion and PFGE. Results All strains of S. sonnei were susceptible to fluoroquinolones (ciprofloxacin and levofloxacin) while 15 out of 123 strains of S. flexneri were resistant. All of the 15 resistant strains displayed common mutations in the gyrA and parC genes and formed eight distinct groups with unique molecular characteristics. Notably, 10 isolates showed mutations at codon 87 of gyrA, and the other 5 were qnrS-positive. Two strains were positive for the aac(6′)-Ib-cr gene. Importantly, this is the first report of qnrS- and aac(6′)-Ib-cr-positive Shigella in China, the qnrS-positive S. flexneri serotypes 1a, 2a and 4c and the aac(6’)-Ib-cr-positive S. flexneri serotypes 2a and 4c worldwide. Conclusions The common mutations at position 83 of gyrA and position 80 of parC were crucial for resistance to nalidixic acid in S. flexneri. The mutation at position 87 of gyrA or the presence of the qnrS gene is necessary for high-level resistance to fluoroquinolones in Shigella isolates from China.</description><subject>aac(6′)-Ib-cr</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>antibiotic resistance</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Bacteria</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Typing Techniques</subject><subject>Biological and medical sciences</subject><subject>China</subject><subject>Cluster Analysis</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Fingerprinting</subject><subject>DNA Gyrase - genetics</subject><subject>DNA Topoisomerase IV - genetics</subject><subject>Drug resistance</subject><subject>Drug Resistance, Bacterial</subject><subject>Dysentery, Bacillary - microbiology</subject><subject>Electrophoresis, Gel, Pulsed-Field</subject><subject>Fluoroquinolones - pharmacology</subject><subject>Genetics</subject><subject>Genotype</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Microbial Sensitivity Tests</subject><subject>Mutation</subject><subject>Mutation, Missense</subject><subject>Pharmacology. Drug treatments</subject><subject>plasmid replicon</subject><subject>Plasmids</subject><subject>qnrS</subject><subject>Shigella flexneri</subject><subject>Shigella flexneri - drug effects</subject><subject>Shigella flexneri - isolation & purification</subject><subject>Shigella sonnei</subject><subject>Shigella sonnei - drug effects</subject><subject>Shigella sonnei - isolation & purification</subject><issn>0305-7453</issn><issn>1460-2091</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c9rFDEUB_Agil2rF_8AGQQ9CGPzO5NjXawr1ApWQb2EN5lMN9vZZE1moPavN8MuLXjQUw75vJf38kXoOcFvCdbsZAP2pLve4UY9QAvCJa4p1uQhWmCGRa24YEfoSc4bjLEUsnmMjoimWjHVLNCP5RoS2NElfwujj6GKfdUPU0zx1-RDHGJwdXLZ5xHCWF2u_ZUbBijE3YRSVPlQrSBc3a7jVEFyMNcv1z7AU_SohyG7Z4fzGH07e_91uarPP3_4uDw9r63gbKwpKCEdx5QLQjEo4KzntoeGWWq51dpZ11ClMXOiXEstu1Z1TadagVlLW3aMXu_77uaRXR7N1mc7DxlcnLKRihDJmfwvpFhoKqgq8OVfcBOnFMoShhIlJcdkRm_2yKaYc3K92SW_hfTbEGzmWEyJxexjKfjFoePUbl13Tw85FPDqACBbGPoEwfp85yjhiuFG37s47f79YL13JTd3cychXZf_YEqY1fef5p38pM6-XFyYS_YHOiqxnA</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>Pu, Xiao-Ying</creator><creator>Pan, Jing-Cao</creator><creator>Wang, Hao-Qiu</creator><creator>Zhang, Wei</creator><creator>Huang, Zhi-Cheng</creator><creator>Gu, Yang-Ming</creator><general>Oxford University Press</general><general>Oxford Publishing Limited (England)</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7T7</scope><scope>7U7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>NAPCQ</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090501</creationdate><title>Characterization of fluoroquinolone-resistant Shigella flexneri in Hangzhou area of China</title><author>Pu, Xiao-Ying ; Pan, Jing-Cao ; Wang, Hao-Qiu ; Zhang, Wei ; Huang, Zhi-Cheng ; Gu, Yang-Ming</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c543t-2a756e40245120a7a43f4cfa83c2c4c99ece827903e50a7696db7d8d7b503b2b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>aac(6′)-Ib-cr</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>antibiotic resistance</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Bacteria</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Typing Techniques</topic><topic>Biological and medical sciences</topic><topic>China</topic><topic>Cluster Analysis</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>DNA Fingerprinting</topic><topic>DNA Gyrase - genetics</topic><topic>DNA Topoisomerase IV - genetics</topic><topic>Drug resistance</topic><topic>Drug Resistance, Bacterial</topic><topic>Dysentery, Bacillary - microbiology</topic><topic>Electrophoresis, Gel, Pulsed-Field</topic><topic>Fluoroquinolones - pharmacology</topic><topic>Genetics</topic><topic>Genotype</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Microbial Sensitivity Tests</topic><topic>Mutation</topic><topic>Mutation, Missense</topic><topic>Pharmacology. Drug treatments</topic><topic>plasmid replicon</topic><topic>Plasmids</topic><topic>qnrS</topic><topic>Shigella flexneri</topic><topic>Shigella flexneri - drug effects</topic><topic>Shigella flexneri - isolation & purification</topic><topic>Shigella sonnei</topic><topic>Shigella sonnei - drug effects</topic><topic>Shigella sonnei - isolation & purification</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pu, Xiao-Ying</creatorcontrib><creatorcontrib>Pan, Jing-Cao</creatorcontrib><creatorcontrib>Wang, Hao-Qiu</creatorcontrib><creatorcontrib>Zhang, Wei</creatorcontrib><creatorcontrib>Huang, Zhi-Cheng</creatorcontrib><creatorcontrib>Gu, Yang-Ming</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Nursing & Allied Health Premium</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of antimicrobial chemotherapy</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pu, Xiao-Ying</au><au>Pan, Jing-Cao</au><au>Wang, Hao-Qiu</au><au>Zhang, Wei</au><au>Huang, Zhi-Cheng</au><au>Gu, Yang-Ming</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of fluoroquinolone-resistant Shigella flexneri in Hangzhou area of China</atitle><jtitle>Journal of antimicrobial chemotherapy</jtitle><addtitle>J Antimicrob Chemother</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>63</volume><issue>5</issue><spage>917</spage><epage>920</epage><pages>917-920</pages><issn>0305-7453</issn><eissn>1460-2091</eissn><coden>JACHDX</coden><abstract>Objectives The aim of this study was to characterize fluoroquinolone-resistant Shigella and determine whether the qnr and aac(6′)-Ib-cr genes could contribute to sporadic shigellosis at the clinic in the Hangzhou area of China. Methods A total of 202 strains of Shigella (79 Shigella sonnei and 123 Shigella flexneri ) isolated from sporadic cases of shigellosis from 1998 to 2007 were analysed for their antimicrobial susceptibility. The gyrA, gyrB, parC, parE, qnr and aac(6′)-Ib-cr genes and the profiles and incompatibility of plasmids were characterized. Chromosomal DNA fingerprinting was determined by XbaI-based digestion and PFGE. Results All strains of S. sonnei were susceptible to fluoroquinolones (ciprofloxacin and levofloxacin) while 15 out of 123 strains of S. flexneri were resistant. All of the 15 resistant strains displayed common mutations in the gyrA and parC genes and formed eight distinct groups with unique molecular characteristics. Notably, 10 isolates showed mutations at codon 87 of gyrA, and the other 5 were qnrS-positive. Two strains were positive for the aac(6′)-Ib-cr gene. Importantly, this is the first report of qnrS- and aac(6′)-Ib-cr-positive Shigella in China, the qnrS-positive S. flexneri serotypes 1a, 2a and 4c and the aac(6’)-Ib-cr-positive S. flexneri serotypes 2a and 4c worldwide. Conclusions The common mutations at position 83 of gyrA and position 80 of parC were crucial for resistance to nalidixic acid in S. flexneri. The mutation at position 87 of gyrA or the presence of the qnrS gene is necessary for high-level resistance to fluoroquinolones in Shigella isolates from China.</abstract><cop>Oxford</cop><pub>Oxford University Press</pub><pmid>19297378</pmid><doi>10.1093/jac/dkp087</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | aac(6′)-Ib-cr Anti-Bacterial Agents - pharmacology antibiotic resistance Antibiotics. Antiinfectious agents. Antiparasitic agents Bacteria Bacterial Proteins - genetics Bacterial Typing Techniques Biological and medical sciences China Cluster Analysis Deoxyribonucleic acid DNA DNA Fingerprinting DNA Gyrase - genetics DNA Topoisomerase IV - genetics Drug resistance Drug Resistance, Bacterial Dysentery, Bacillary - microbiology Electrophoresis, Gel, Pulsed-Field Fluoroquinolones - pharmacology Genetics Genotype Humans Medical sciences Microbial Sensitivity Tests Mutation Mutation, Missense Pharmacology. Drug treatments plasmid replicon Plasmids qnrS Shigella flexneri Shigella flexneri - drug effects Shigella flexneri - isolation & purification Shigella sonnei Shigella sonnei - drug effects Shigella sonnei - isolation & purification |
| title | Characterization of fluoroquinolone-resistant Shigella flexneri in Hangzhou area of China |
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