Influence of culture conditions on recombinant Drosophila melanogaster S2 cells producing rabies virus glycoprotein cultivated in serum-free medium

The recombinant G glycoprotein from the surface of the rabies virus (RVGP) is a promising candidate as a rabies vaccine component and also for diagnostic purposes. In this study, RVGP production by transfected Drosophila melanogaster S2 cells cultivated in a serum-free medium (supplemented IPL-41 me...

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Veröffentlicht in:	Biologicals 2009-04, Vol.37 (2), p.108-118
Hauptverfasser:	Batista, Fabiana R.X., Moraes, Ângela M., Büntemeyer, Heino, Noll, Thomas
Format:	Artikel
Sprache:	eng
Schlagworte:	Aeration Amino Acids - metabolism Animals Carbohydrate Metabolism - drug effects Cell Culture Techniques Cell Line - metabolism Cell Line - physiology Cell Proliferation - drug effects Culture Media, Serum-Free - pharmacology Drosophila melanogaster Fed-batch culture Growth kinetics Hydrogen-Ion Concentration Lactic Acid - metabolism Oxygen Consumption - drug effects Rabies virus Rabies virus - genetics Rabies virus - metabolism Recombinant Proteins - genetics Recombinant Proteins - metabolism RVGP S2 cells Time Factors Viral Fusion Proteins - isolation & purification Viral Fusion Proteins - metabolism
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