Detection of stx1 , stx2 , and eae genes of enterohemorrhagic Escherichia coli using SYBR Green in a real-time polymerase chain reaction

Abstract We report a multiplex real-time polymerase chain reaction method for detecting enterohemorrhagic Escherichia coli (EHEC) from strains or stool specimens. This assay detected the virulence genes stx1 , stx2 , and eae , without the use of probes. The method, which was validated on a collectio...

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Veröffentlicht in:	Diagnostic microbiology and infectious disease 2009-05, Vol.64 (1), p.98-101
Hauptverfasser:	Chassagne, Laurent, Pradel, Nathalie, Robin, Frédéric, Livrelli, Valérie, Bonnet, Richard, Delmas, Julien
Format:	Artikel
Sprache:	eng
Schlagworte:	Adhesins, Bacterial - genetics Child eae EHEC Enterohemorrhagic Escherichia coli - genetics Escherichia coli Escherichia coli Infections - microbiology Escherichia coli Proteins - genetics Feces - microbiology Humans Infectious Disease Internal Medicine Organic Chemicals - metabolism PCR Polymerase Chain Reaction - methods Real-time Shiga Toxin 1 - genetics Shiga Toxin 2 - genetics Staining and Labeling STEC Stools stx SYBR Green Virulence Factors - genetics
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Zusammenfassung:	Abstract We report a multiplex real-time polymerase chain reaction method for detecting enterohemorrhagic Escherichia coli (EHEC) from strains or stool specimens. This assay detected the virulence genes stx1 , stx2 , and eae , without the use of probes. The method, which was validated on a collection of 143 EHEC strains, is simple, rapid, cost-effective, and sensitive.
ISSN:	0732-8893 1879-0070
DOI:	10.1016/j.diagmicrobio.2009.01.031