Urinary steroids in young women with eating disorders

Urinary steroid components were measured after enzyme hydrolysis and methoxym-silyl derivatization by capillary gas chromatography in young women with eating disorders. Using three internal standards, programmed temperature from 50 to 300 °C and flame ionization detection, on ULTRA-1 capillary colum...

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Veröffentlicht in:	Journal of biochemical and biophysical methods 2004-10, Vol.61 (1), p.199-205
Hauptverfasser:	Poór, Viktória, Bíró, Ildikó, Bufa, Anita, Gáti, Ágnes, Fenyvesi, Ilona, Juricskay, Susan, Tényi, Tamás, Kilár, Ferenc
Format:	Artikel
Sprache:	eng
Schlagworte:	Adult Biomarkers - urine Capillary gas chromatography Chromatography, Gas - methods Cortisol Dehydroepiandrosterone Eating disorders Female Humans Steroids - urine Urinalysis - methods Urinary steroids Women's Health
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Beschreibung
Zusammenfassung:	Urinary steroid components were measured after enzyme hydrolysis and methoxym-silyl derivatization by capillary gas chromatography in young women with eating disorders. Using three internal standards, programmed temperature from 50 to 300 °C and flame ionization detection, on ULTRA-1 capillary column the separation of 28 steroid components is possible. Fifteen young women with different eating disorders, i.e. anorexia nervosa, bulimia nervosa and bulimarexia (aged 16–28 years) and 15 healthy women (aged 22–26 years) collected 24-h urine. A significant decrease of dehydroepiandrosterone ( p
ISSN:	0165-022X 1872-857X
DOI:	10.1016/j.jbbm.2004.09.004