Nuclear Progesterone Receptor Expression in the Human Fetal Membranes and Decidua at Term Before and After Labor
To explore how progesterone affects human pregnancy, we identified the progesterone target cells within the fetal membranes (amnion, chorion, and decidua) at term by assessing the extent of expression and localization of the nuclear progesterone receptors, progesterone receptor-A and progesterone re...
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| Veröffentlicht in: | Reproductive sciences (Thousand Oaks, Calif.) Calif.), 2009-04, Vol.16 (4), p.357-363 |
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| container_title | Reproductive sciences (Thousand Oaks, Calif.) |
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| creator | Merlino, Amy Welsh, Toni Erdonmez, Tan Madsen, Gemma Zakar, Tamas Smith, Roger Mercer, Brian Mesiano, Sam |
| description | To explore how progesterone affects human pregnancy, we identified the progesterone target cells within the fetal membranes (amnion, chorion, and decidua) at term by assessing the extent of expression and localization of the nuclear progesterone receptors, progesterone receptor-A and progesterone receptor-B. Fetal membranes (separated into amnion and chorion—decidua) were obtained after term cesarean deliveries performed before (n = 7) and after (n = 7) labor onset. Nuclear progesterone receptor expression was determined by the abundance of nuclear progesterone receptor mRNAs (by quantitative reverse transcriptase—polymerase chain reaction) and proteins (by western blotting). Localization of nPRs was determined by immunohistochemistry. Progesterone receptor-A and progesterone receptor-B mRNA and protein levels were highest in the chorion—decidua and did not change in association with labor. Nuclear progesterone receptor mRNAs and proteins were barely detectable in amnion. Nuclear progesterone receptor immunostaining was detected only in the nucleus of decidual cells. These findings suggest that the decidua, and not the amnion and chorion, is a direct target for nuclear progesterone receptor—mediated progesterone actions during human pregnancy. |
| doi_str_mv | 10.1177/1933719108328616 |
| format | Article |
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Fetal membranes (separated into amnion and chorion—decidua) were obtained after term cesarean deliveries performed before (n = 7) and after (n = 7) labor onset. Nuclear progesterone receptor expression was determined by the abundance of nuclear progesterone receptor mRNAs (by quantitative reverse transcriptase—polymerase chain reaction) and proteins (by western blotting). Localization of nPRs was determined by immunohistochemistry. Progesterone receptor-A and progesterone receptor-B mRNA and protein levels were highest in the chorion—decidua and did not change in association with labor. Nuclear progesterone receptor mRNAs and proteins were barely detectable in amnion. Nuclear progesterone receptor immunostaining was detected only in the nucleus of decidual cells. These findings suggest that the decidua, and not the amnion and chorion, is a direct target for nuclear progesterone receptor—mediated progesterone actions during human pregnancy.</description><identifier>ISSN: 1933-7191</identifier><identifier>EISSN: 1933-7205</identifier><identifier>DOI: 10.1177/1933719108328616</identifier><identifier>PMID: 19196877</identifier><language>eng</language><publisher>Los Angeles, CA: SAGE Publications</publisher><subject>Amnion - chemistry ; Biological and medical sciences ; Blotting, Western ; Cell Nucleus - chemistry ; Chorion - chemistry ; Decidua - chemistry ; Delivery. Postpartum. Lactation ; Embryology ; Extraembryonic Membranes - chemistry ; Female ; Gene Expression ; Gynecology. Andrology. Obstetrics ; Humans ; Immunohistochemistry ; Labor, Obstetric ; Medical sciences ; Medicine & Public Health ; Obstetrics/Perinatology/Midwifery ; Parturition - metabolism ; Pregnancy ; Receptors, Progesterone - analysis ; Receptors, Progesterone - genetics ; Reproductive Medicine ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - analysis</subject><ispartof>Reproductive sciences (Thousand Oaks, Calif.), 2009-04, Vol.16 (4), p.357-363</ispartof><rights>Society for Reproductive Investigation 2012</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c473t-7952d6bf0bbc17798b39cf5a963883d8d949724a94a3ecc9f59361e76b0a3aed3</citedby><cites>FETCH-LOGICAL-c473t-7952d6bf0bbc17798b39cf5a963883d8d949724a94a3ecc9f59361e76b0a3aed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1177/1933719108328616$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1177/1933719108328616$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,776,780,21798,27901,27902,41464,42533,43597,43598,51294</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21304631$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19196877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Merlino, Amy</creatorcontrib><creatorcontrib>Welsh, Toni</creatorcontrib><creatorcontrib>Erdonmez, Tan</creatorcontrib><creatorcontrib>Madsen, Gemma</creatorcontrib><creatorcontrib>Zakar, Tamas</creatorcontrib><creatorcontrib>Smith, Roger</creatorcontrib><creatorcontrib>Mercer, Brian</creatorcontrib><creatorcontrib>Mesiano, Sam</creatorcontrib><title>Nuclear Progesterone Receptor Expression in the Human Fetal Membranes and Decidua at Term Before and After Labor</title><title>Reproductive sciences (Thousand Oaks, Calif.)</title><addtitle>Reprod. Sci</addtitle><addtitle>Reprod Sci</addtitle><description>To explore how progesterone affects human pregnancy, we identified the progesterone target cells within the fetal membranes (amnion, chorion, and decidua) at term by assessing the extent of expression and localization of the nuclear progesterone receptors, progesterone receptor-A and progesterone receptor-B. Fetal membranes (separated into amnion and chorion—decidua) were obtained after term cesarean deliveries performed before (n = 7) and after (n = 7) labor onset. Nuclear progesterone receptor expression was determined by the abundance of nuclear progesterone receptor mRNAs (by quantitative reverse transcriptase—polymerase chain reaction) and proteins (by western blotting). Localization of nPRs was determined by immunohistochemistry. Progesterone receptor-A and progesterone receptor-B mRNA and protein levels were highest in the chorion—decidua and did not change in association with labor. Nuclear progesterone receptor mRNAs and proteins were barely detectable in amnion. Nuclear progesterone receptor immunostaining was detected only in the nucleus of decidual cells. These findings suggest that the decidua, and not the amnion and chorion, is a direct target for nuclear progesterone receptor—mediated progesterone actions during human pregnancy.</description><subject>Amnion - chemistry</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Cell Nucleus - chemistry</subject><subject>Chorion - chemistry</subject><subject>Decidua - chemistry</subject><subject>Delivery. Postpartum. Lactation</subject><subject>Embryology</subject><subject>Extraembryonic Membranes - chemistry</subject><subject>Female</subject><subject>Gene Expression</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Labor, Obstetric</subject><subject>Medical sciences</subject><subject>Medicine & Public Health</subject><subject>Obstetrics/Perinatology/Midwifery</subject><subject>Parturition - metabolism</subject><subject>Pregnancy</subject><subject>Receptors, Progesterone - analysis</subject><subject>Receptors, Progesterone - genetics</subject><subject>Reproductive Medicine</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - analysis</subject><issn>1933-7191</issn><issn>1933-7205</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkEtPhTAQhRuj8b13ZbrRHdpSaOnStybXR4yuyVCGKwYotpDov7d6URMXxlWbOd857RxCdjg74FypQ66FUFxzlok4k1wukfWPUaRili5_3YO-Rja8f2YsTXScrZK1MNIyU2qd9DejaRAcvXN2jn5AZzuk92iwH6yjZ6-9Q-9r29G6o8MT0suxhY6e4wANvca2cNChp9CV9BRNXY5AYaAP6Fp6jJV1-CkdVSGYzqCwbousVNB43J7OTfJ4fvZwchnNbi-uTo5mkUmUGCKl07iURcWKwoRNdVYIbaoUtBRZJsqs1IlWcQI6AYHG6CrVQnJUsmAgAEuxSfYXub2zL2PYLG9rb7Bpwn_t6HOpOJOKqQCyBWic9d5hlfeubsG95ZzlHy3nv1sOlt0peyxaLH8MU60B2JsA8AaaKpRkav_NxVywRAoeOL7gfJC6Obr82Y6uC7389Xg0eWCO_-DfAW4PoBM</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Merlino, Amy</creator><creator>Welsh, Toni</creator><creator>Erdonmez, Tan</creator><creator>Madsen, Gemma</creator><creator>Zakar, Tamas</creator><creator>Smith, Roger</creator><creator>Mercer, Brian</creator><creator>Mesiano, Sam</creator><general>SAGE Publications</general><general>Springer International Publishing</general><general>Sage Publications</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090401</creationdate><title>Nuclear Progesterone Receptor Expression in the Human Fetal Membranes and Decidua at Term Before and After Labor</title><author>Merlino, Amy ; Welsh, Toni ; Erdonmez, Tan ; Madsen, Gemma ; Zakar, Tamas ; Smith, Roger ; Mercer, Brian ; Mesiano, Sam</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c473t-7952d6bf0bbc17798b39cf5a963883d8d949724a94a3ecc9f59361e76b0a3aed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amnion - chemistry</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Cell Nucleus - chemistry</topic><topic>Chorion - chemistry</topic><topic>Decidua - chemistry</topic><topic>Delivery. Postpartum. Lactation</topic><topic>Embryology</topic><topic>Extraembryonic Membranes - chemistry</topic><topic>Female</topic><topic>Gene Expression</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Labor, Obstetric</topic><topic>Medical sciences</topic><topic>Medicine & Public Health</topic><topic>Obstetrics/Perinatology/Midwifery</topic><topic>Parturition - metabolism</topic><topic>Pregnancy</topic><topic>Receptors, Progesterone - analysis</topic><topic>Receptors, Progesterone - genetics</topic><topic>Reproductive Medicine</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - analysis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Merlino, Amy</creatorcontrib><creatorcontrib>Welsh, Toni</creatorcontrib><creatorcontrib>Erdonmez, Tan</creatorcontrib><creatorcontrib>Madsen, Gemma</creatorcontrib><creatorcontrib>Zakar, Tamas</creatorcontrib><creatorcontrib>Smith, Roger</creatorcontrib><creatorcontrib>Mercer, Brian</creatorcontrib><creatorcontrib>Mesiano, Sam</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Reproductive sciences (Thousand Oaks, Calif.)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Merlino, Amy</au><au>Welsh, Toni</au><au>Erdonmez, Tan</au><au>Madsen, Gemma</au><au>Zakar, Tamas</au><au>Smith, Roger</au><au>Mercer, Brian</au><au>Mesiano, Sam</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear Progesterone Receptor Expression in the Human Fetal Membranes and Decidua at Term Before and After Labor</atitle><jtitle>Reproductive sciences (Thousand Oaks, Calif.)</jtitle><stitle>Reprod. Sci</stitle><addtitle>Reprod Sci</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>16</volume><issue>4</issue><spage>357</spage><epage>363</epage><pages>357-363</pages><issn>1933-7191</issn><eissn>1933-7205</eissn><abstract>To explore how progesterone affects human pregnancy, we identified the progesterone target cells within the fetal membranes (amnion, chorion, and decidua) at term by assessing the extent of expression and localization of the nuclear progesterone receptors, progesterone receptor-A and progesterone receptor-B. Fetal membranes (separated into amnion and chorion—decidua) were obtained after term cesarean deliveries performed before (n = 7) and after (n = 7) labor onset. Nuclear progesterone receptor expression was determined by the abundance of nuclear progesterone receptor mRNAs (by quantitative reverse transcriptase—polymerase chain reaction) and proteins (by western blotting). Localization of nPRs was determined by immunohistochemistry. Progesterone receptor-A and progesterone receptor-B mRNA and protein levels were highest in the chorion—decidua and did not change in association with labor. Nuclear progesterone receptor mRNAs and proteins were barely detectable in amnion. Nuclear progesterone receptor immunostaining was detected only in the nucleus of decidual cells. These findings suggest that the decidua, and not the amnion and chorion, is a direct target for nuclear progesterone receptor—mediated progesterone actions during human pregnancy.</abstract><cop>Los Angeles, CA</cop><pub>SAGE Publications</pub><pmid>19196877</pmid><doi>10.1177/1933719108328616</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; SAGE Complete; Alma/SFX Local Collection |
| subjects | Amnion - chemistry Biological and medical sciences Blotting, Western Cell Nucleus - chemistry Chorion - chemistry Decidua - chemistry Delivery. Postpartum. Lactation Embryology Extraembryonic Membranes - chemistry Female Gene Expression Gynecology. Andrology. Obstetrics Humans Immunohistochemistry Labor, Obstetric Medical sciences Medicine & Public Health Obstetrics/Perinatology/Midwifery Parturition - metabolism Pregnancy Receptors, Progesterone - analysis Receptors, Progesterone - genetics Reproductive Medicine Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - analysis |
| title | Nuclear Progesterone Receptor Expression in the Human Fetal Membranes and Decidua at Term Before and After Labor |
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