TIRF microscopy evanescent field calibration using tilted fluorescent microtubules

Total internal reflection fluorescence microscopy has become a powerful tool to study the dynamics of sub-cellular structures and single molecules near substrate surfaces. However, the penetration depth of the evanescent field, that is, the distance at which the excitation intensity has exponentiall...

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Veröffentlicht in:	Journal of microscopy (Oxford) 2009-04, Vol.234 (1), p.38-46
Hauptverfasser:	GELL, C, BERNDT, M, ENDERLEIN, J, DIEZ, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Brain Chemistry Calibration Evanescent field microscope calibration Microscopy, Fluorescence - methods Microscopy, Fluorescence - standards Microtubules penetration depth Swine TIRF Tubulin - isolation & purification
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