Combined Smooth Muscle and Melanocytic Differentiation in Lymphangioleiomyomatosis
Pulmonary lymphangioleiomyomatosis (LAM) is characterized by abnormal proliferation of immature-looking smooth muscle (SM)-like cells (LAM cells), leading to lung destruction and cyst formation. In addition to expressing some SM markers, scattered LAM cells express the melanocytic maker gp100, which...
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description | Pulmonary lymphangioleiomyomatosis (LAM) is characterized by abnormal proliferation of immature-looking smooth muscle (SM)-like cells (LAM cells), leading to lung destruction and cyst formation. In addition to expressing some SM markers, scattered LAM cells express the melanocytic maker gp100, which is recognized by antibody HMB45, suggesting that at least a few LAM cells may have melanocytic differentiation. Here we immunostained 26 LAM samples for several melanocyte-related proteins. These studies showed that all LAM cells express tetraspanin CD63, a melanoma-associated protein that belongs to the transmembrane 4 superfamily. The majority of LAM cells also immunoreacted with PNL2, an antibody against a yet uncharacterized melanocytic antigen. Furthermore, we examined the co-expression of PNL2 and Ki-67, an indicator of cell proliferation, and found that PNL2-positive LAM cells showed a significantly lower proliferation rate compared with their negative counterparts. Our findings shed new light on the nature of the LAM cells by demonstrating their combined SM and melanocytic differentiation and the existence of subpopulations with different proliferative potential. Furthermore, these studies provided two new antibodies useful in the diagnosis of LAM. |
doi_str_mv | 10.1369/jhc.4A6438.2004 |
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In addition to expressing some SM markers, scattered LAM cells express the melanocytic maker gp100, which is recognized by antibody HMB45, suggesting that at least a few LAM cells may have melanocytic differentiation. Here we immunostained 26 LAM samples for several melanocyte-related proteins. These studies showed that all LAM cells express tetraspanin CD63, a melanoma-associated protein that belongs to the transmembrane 4 superfamily. The majority of LAM cells also immunoreacted with PNL2, an antibody against a yet uncharacterized melanocytic antigen. Furthermore, we examined the co-expression of PNL2 and Ki-67, an indicator of cell proliferation, and found that PNL2-positive LAM cells showed a significantly lower proliferation rate compared with their negative counterparts. Our findings shed new light on the nature of the LAM cells by demonstrating their combined SM and melanocytic differentiation and the existence of subpopulations with different proliferative potential. Furthermore, these studies provided two new antibodies useful in the diagnosis of LAM.</description><identifier>ISSN: 0022-1554</identifier><identifier>EISSN: 1551-5044</identifier><identifier>DOI: 10.1369/jhc.4A6438.2004</identifier><identifier>PMID: 15557209</identifier><language>eng</language><publisher>Los Angeles, CA: Histochemical Soc</publisher><subject>Actins - biosynthesis ; Adult ; Aged ; Antigens, CD - biosynthesis ; Cell Differentiation ; Cell Proliferation ; Humans ; Immunohistochemistry ; Ki-67 Antigen - biosynthesis ; Lung Neoplasms - pathology ; Lymphangioleiomyomatosis - pathology ; Melanocytes - metabolism ; Melanocytes - pathology ; Middle Aged ; Myocytes, Smooth Muscle - metabolism ; Myocytes, Smooth Muscle - pathology ; Platelet Membrane Glycoproteins - biosynthesis ; Tetraspanin 30</subject><ispartof>The journal of histochemistry and cytochemistry, 2004-12, Vol.52 (12), p.1537-1542</ispartof><rights>2004 Authors</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c364t-ed3ea186e33dfae23ee3e64b1df39fd5f31fdbe4eb48b97fc8d7ef6c02918f623</citedby><cites>FETCH-LOGICAL-c364t-ed3ea186e33dfae23ee3e64b1df39fd5f31fdbe4eb48b97fc8d7ef6c02918f623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://journals.sagepub.com/doi/pdf/10.1369/jhc.4A6438.2004$$EPDF$$P50$$Gsage$$H</linktopdf><linktohtml>$$Uhttps://journals.sagepub.com/doi/10.1369/jhc.4A6438.2004$$EHTML$$P50$$Gsage$$H</linktohtml><link.rule.ids>314,778,782,21806,27911,27912,43608,43609</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15557209$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhe, Xiaoning</creatorcontrib><creatorcontrib>Schuger, Lucia</creatorcontrib><title>Combined Smooth Muscle and Melanocytic Differentiation in Lymphangioleiomyomatosis</title><title>The journal of histochemistry and cytochemistry</title><addtitle>J Histochem Cytochem</addtitle><description>Pulmonary lymphangioleiomyomatosis (LAM) is characterized by abnormal proliferation of immature-looking smooth muscle (SM)-like cells (LAM cells), leading to lung destruction and cyst formation. In addition to expressing some SM markers, scattered LAM cells express the melanocytic maker gp100, which is recognized by antibody HMB45, suggesting that at least a few LAM cells may have melanocytic differentiation. Here we immunostained 26 LAM samples for several melanocyte-related proteins. These studies showed that all LAM cells express tetraspanin CD63, a melanoma-associated protein that belongs to the transmembrane 4 superfamily. The majority of LAM cells also immunoreacted with PNL2, an antibody against a yet uncharacterized melanocytic antigen. Furthermore, we examined the co-expression of PNL2 and Ki-67, an indicator of cell proliferation, and found that PNL2-positive LAM cells showed a significantly lower proliferation rate compared with their negative counterparts. Our findings shed new light on the nature of the LAM cells by demonstrating their combined SM and melanocytic differentiation and the existence of subpopulations with different proliferative potential. Furthermore, these studies provided two new antibodies useful in the diagnosis of LAM.</description><subject>Actins - biosynthesis</subject><subject>Adult</subject><subject>Aged</subject><subject>Antigens, CD - biosynthesis</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Humans</subject><subject>Immunohistochemistry</subject><subject>Ki-67 Antigen - biosynthesis</subject><subject>Lung Neoplasms - pathology</subject><subject>Lymphangioleiomyomatosis - pathology</subject><subject>Melanocytes - metabolism</subject><subject>Melanocytes - pathology</subject><subject>Middle Aged</subject><subject>Myocytes, Smooth Muscle - metabolism</subject><subject>Myocytes, Smooth Muscle - pathology</subject><subject>Platelet Membrane Glycoproteins - biosynthesis</subject><subject>Tetraspanin 30</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtLAzEQh4MotlbP3mRPenFrssm-jlKfUBF8nEM2mXRTNpu62aX0vzdlC96EgYHhmx8zH0KXBM8Jzcq7dS3n7D5jtJgnGLMjNCVpSuIUM3aMphgnSRwGbILOvF9jTBhLi1M0CbM0T3A5RR8LZyvTgoo-rXN9Hb0NXjYQiVZFb9CI1sldb2T0YLSGDtreiN64NjJttNzZTS3alXENGGd3zoreeePP0YkWjYeLQ5-h76fHr8VLvHx_fl3cL2NJM9bHoCgIUmRAqdICEgpAIWMVUZqWWqWaEq0qYFCxoipzLQuVg84kTkpS6CyhM3Q95m469zOA77k1XkITjgY3eJ7luCzDkwG8G0HZOe870HzTGSu6HSeY7zXyoJGPGvleY9i4OkQPlQX1xx-8BeB2BLxYAV-7oWvDq__k3Yx4bVb11nTAvRVNE9IJ3263acJJqJTm9BduBowL</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>Zhe, Xiaoning</creator><creator>Schuger, Lucia</creator><general>Histochemical Soc</general><general>SAGE Publications</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041201</creationdate><title>Combined Smooth Muscle and Melanocytic Differentiation in Lymphangioleiomyomatosis</title><author>Zhe, Xiaoning ; Schuger, Lucia</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c364t-ed3ea186e33dfae23ee3e64b1df39fd5f31fdbe4eb48b97fc8d7ef6c02918f623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Actins - biosynthesis</topic><topic>Adult</topic><topic>Aged</topic><topic>Antigens, CD - biosynthesis</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Humans</topic><topic>Immunohistochemistry</topic><topic>Ki-67 Antigen - biosynthesis</topic><topic>Lung Neoplasms - pathology</topic><topic>Lymphangioleiomyomatosis - pathology</topic><topic>Melanocytes - metabolism</topic><topic>Melanocytes - pathology</topic><topic>Middle Aged</topic><topic>Myocytes, Smooth Muscle - metabolism</topic><topic>Myocytes, Smooth Muscle - pathology</topic><topic>Platelet Membrane Glycoproteins - biosynthesis</topic><topic>Tetraspanin 30</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhe, Xiaoning</creatorcontrib><creatorcontrib>Schuger, Lucia</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhe, Xiaoning</au><au>Schuger, Lucia</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Combined Smooth Muscle and Melanocytic Differentiation in Lymphangioleiomyomatosis</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>2004-12-01</date><risdate>2004</risdate><volume>52</volume><issue>12</issue><spage>1537</spage><epage>1542</epage><pages>1537-1542</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><abstract>Pulmonary lymphangioleiomyomatosis (LAM) is characterized by abnormal proliferation of immature-looking smooth muscle (SM)-like cells (LAM cells), leading to lung destruction and cyst formation. In addition to expressing some SM markers, scattered LAM cells express the melanocytic maker gp100, which is recognized by antibody HMB45, suggesting that at least a few LAM cells may have melanocytic differentiation. Here we immunostained 26 LAM samples for several melanocyte-related proteins. These studies showed that all LAM cells express tetraspanin CD63, a melanoma-associated protein that belongs to the transmembrane 4 superfamily. The majority of LAM cells also immunoreacted with PNL2, an antibody against a yet uncharacterized melanocytic antigen. Furthermore, we examined the co-expression of PNL2 and Ki-67, an indicator of cell proliferation, and found that PNL2-positive LAM cells showed a significantly lower proliferation rate compared with their negative counterparts. Our findings shed new light on the nature of the LAM cells by demonstrating their combined SM and melanocytic differentiation and the existence of subpopulations with different proliferative potential. Furthermore, these studies provided two new antibodies useful in the diagnosis of LAM.</abstract><cop>Los Angeles, CA</cop><pub>Histochemical Soc</pub><pmid>15557209</pmid><doi>10.1369/jhc.4A6438.2004</doi><tpages>6</tpages></addata></record> |
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subjects | Actins - biosynthesis Adult Aged Antigens, CD - biosynthesis Cell Differentiation Cell Proliferation Humans Immunohistochemistry Ki-67 Antigen - biosynthesis Lung Neoplasms - pathology Lymphangioleiomyomatosis - pathology Melanocytes - metabolism Melanocytes - pathology Middle Aged Myocytes, Smooth Muscle - metabolism Myocytes, Smooth Muscle - pathology Platelet Membrane Glycoproteins - biosynthesis Tetraspanin 30 |
title | Combined Smooth Muscle and Melanocytic Differentiation in Lymphangioleiomyomatosis |
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