Immunolocalization of Actin in Paramecium Cells

We have selected a conserved immunogenic region from several actin genes of Paramecium, recently cloned in our laboratory, to prepare antibodies for Western blots and immunolocalization. According to cell fractionation analysis, most actin is structure-bound. Immunofluorescence shows signal enriched...

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Veröffentlicht in:	The journal of histochemistry and cytochemistry 2004-12, Vol.52 (12), p.1543-1559
Hauptverfasser:	Kissmehl, Roland, Sehring, Ivonne M, Wagner, Erika, Plattner, Helmut
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins - genetics Actins - immunology Actins - metabolism Amino Acid Sequence Animals Antibody Specificity Blotting, Western Cell Fractionation Immunohistochemistry Molecular Sequence Data Paramecium - metabolism Paramecium - ultrastructure Sequence Alignment
Online-Zugang:	Volltext
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