The pur6 gene of the puromycin biosynthetic gene cluster from Streptomyces alboniger encodes a tyrosinyl-aminonucleoside synthetase
The pur6 gene of the puromycin biosynthetic gene ( pur) cluster from Streptomyces alboniger is shown to be essential for puromycin biosynthesis. Cell lysates from this mycelial bacterium were active in linking l-tyrosine to both 3 ′-amino-3 ′-deoxyadenosine and N 6, N 6-dimethyl-3 ′-amino-3 ′-deoxya...
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| Veröffentlicht in: | FEBS letters 2004-11, Vol.577 (3), p.371-375 |
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| creator | Ángel Rubio, Miguel Barrado, Patricia Carlos Espinosa, Juan Jiménez, Antonio Lobato, Marı́a Fernández |
| description | The
pur6 gene of the puromycin biosynthetic gene (
pur) cluster from
Streptomyces alboniger is shown to be essential for puromycin biosynthesis. Cell lysates from this mycelial bacterium were active in linking
l-tyrosine to both 3
′-amino-3
′-deoxyadenosine and
N
6,
N
6-dimethyl-3
′-amino-3
′-deoxyadenosine with a peptide-like bond. Identical reactions were performed by cell lysates from
Streptomyces lividans or
Escherichia coli transformants that expressed
pur6 from a variety of plasmid constructs. Physicochemical and biochemical analyses suggested that their products were tridemethyl puromycin and
O-demethylpuromycin, respectively. Therefore, it appears that Pur6 is the tyrosinyl-aminonucleoside synthetase of the puromycin biosynthetic pathway. |
| doi_str_mv | 10.1016/j.febslet.2004.09.087 |
| format | Article |
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pur6 gene of the puromycin biosynthetic gene (
pur) cluster from
Streptomyces alboniger is shown to be essential for puromycin biosynthesis. Cell lysates from this mycelial bacterium were active in linking
l-tyrosine to both 3
′-amino-3
′-deoxyadenosine and
N
6,
N
6-dimethyl-3
′-amino-3
′-deoxyadenosine with a peptide-like bond. Identical reactions were performed by cell lysates from
Streptomyces lividans or
Escherichia coli transformants that expressed
pur6 from a variety of plasmid constructs. Physicochemical and biochemical analyses suggested that their products were tridemethyl puromycin and
O-demethylpuromycin, respectively. Therefore, it appears that Pur6 is the tyrosinyl-aminonucleoside synthetase of the puromycin biosynthetic pathway.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2004.09.087</identifier><identifier>PMID: 15556612</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Chemistry, Physical - methods ; dA, deoxyadenosine ; Escherichia coli ; Escherichia coli - genetics ; Gene Deletion ; Gene Expression ; Genes, Bacterial ; Kinetics ; Molecular Structure ; Multigene Family ; ODMP, O-demethylpuromycin ; ORF, open reading frame ; Pac, puromycin N-acetyltransferase ; Plasmids ; pur cluster ; pur6 gene ; Puromycin - biosynthesis ; Puromycin Aminonucleoside - chemistry ; Puromycin Aminonucleoside - metabolism ; puromycin aminonucleoside, N 6,N 6-dimethyl-3′-amino-3′-deoxyadenosine ; Streptomyces ; Streptomyces - genetics ; Streptomyces - metabolism ; Streptomyces alboniger ; Streptomyces lividans ; Streptomyces lividans - genetics ; TDMP, tridemethylpuromycin ; Tyrosinyl-aminonucleoside synthetase</subject><ispartof>FEBS letters, 2004-11, Vol.577 (3), p.371-375</ispartof><rights>2004</rights><rights>FEBS Letters 577 (2004) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4572-a339d68fac587bfede198aa0a6963afeb1805e8342c238eb1603be19f4e17ad73</citedby><cites>FETCH-LOGICAL-c4572-a339d68fac587bfede198aa0a6963afeb1805e8342c238eb1603be19f4e17ad73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2004.09.087$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.febslet.2004.09.087$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1417,1433,3550,27924,27925,45574,45575,45995,46409,46833</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15556612$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ángel Rubio, Miguel</creatorcontrib><creatorcontrib>Barrado, Patricia</creatorcontrib><creatorcontrib>Carlos Espinosa, Juan</creatorcontrib><creatorcontrib>Jiménez, Antonio</creatorcontrib><creatorcontrib>Lobato, Marı́a Fernández</creatorcontrib><title>The pur6 gene of the puromycin biosynthetic gene cluster from Streptomyces alboniger encodes a tyrosinyl-aminonucleoside synthetase</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>The
pur6 gene of the puromycin biosynthetic gene (
pur) cluster from
Streptomyces alboniger is shown to be essential for puromycin biosynthesis. Cell lysates from this mycelial bacterium were active in linking
l-tyrosine to both 3
′-amino-3
′-deoxyadenosine and
N
6,
N
6-dimethyl-3
′-amino-3
′-deoxyadenosine with a peptide-like bond. Identical reactions were performed by cell lysates from
Streptomyces lividans or
Escherichia coli transformants that expressed
pur6 from a variety of plasmid constructs. Physicochemical and biochemical analyses suggested that their products were tridemethyl puromycin and
O-demethylpuromycin, respectively. Therefore, it appears that Pur6 is the tyrosinyl-aminonucleoside synthetase of the puromycin biosynthetic pathway.</description><subject>Chemistry, Physical - methods</subject><subject>dA, deoxyadenosine</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Gene Deletion</subject><subject>Gene Expression</subject><subject>Genes, Bacterial</subject><subject>Kinetics</subject><subject>Molecular Structure</subject><subject>Multigene Family</subject><subject>ODMP, O-demethylpuromycin</subject><subject>ORF, open reading frame</subject><subject>Pac, puromycin N-acetyltransferase</subject><subject>Plasmids</subject><subject>pur cluster</subject><subject>pur6 gene</subject><subject>Puromycin - biosynthesis</subject><subject>Puromycin Aminonucleoside - chemistry</subject><subject>Puromycin Aminonucleoside - metabolism</subject><subject>puromycin aminonucleoside, N 6,N 6-dimethyl-3′-amino-3′-deoxyadenosine</subject><subject>Streptomyces</subject><subject>Streptomyces - genetics</subject><subject>Streptomyces - metabolism</subject><subject>Streptomyces alboniger</subject><subject>Streptomyces lividans</subject><subject>Streptomyces lividans - genetics</subject><subject>TDMP, tridemethylpuromycin</subject><subject>Tyrosinyl-aminonucleoside synthetase</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1TAQRi0Eope2jwDyil2Cf2LHXiGoWopUiUXL2nKcSfFVEl_sBJR1X7yOEollWVkzPv5sz0HoPSUlJVR-OpYdNKmHqWSEVCXRJVH1K3SgquYFr6R6jQ6E0KoQteZn6F1KR5JrRfVbdEaFEFJSdkBPD78An-Yo8SOMgEOHp60RhsX5ETc-pGXMvcm7DXH9nCaIuMsIvp8inKaVhYRt34TRP-Y9GF1o1w6elhiSH5e-sIMfwzi7HnKjBbzH2gQX6E1n-wSX-3qOft5cP1zdFnc_vn2_-nJXuErUrLCc61aqzjqh6qaDFqhW1hIrteQ2T4MqIkDxijnGVS4l4U1mugpobduan6OPW-4pht8zpMkMPjnoeztCmJORNdGKVexFkGqmNREig2IDXf5litCZU_SDjYuhxKyazNHsmsyqyRBtsqZ87sN-wdwM0P47tXvJwO0G_PU9LP-Xam6uv7L71fmqnFSEMqnWqM9bFOTR_vEQTXI-C4LWR3CTaYN_4bXPzS6_wg</recordid><startdate>20041119</startdate><enddate>20041119</enddate><creator>Ángel Rubio, Miguel</creator><creator>Barrado, Patricia</creator><creator>Carlos Espinosa, Juan</creator><creator>Jiménez, Antonio</creator><creator>Lobato, Marı́a Fernández</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20041119</creationdate><title>The pur6 gene of the puromycin biosynthetic gene cluster from Streptomyces alboniger encodes a tyrosinyl-aminonucleoside synthetase</title><author>Ángel Rubio, Miguel ; Barrado, Patricia ; Carlos Espinosa, Juan ; Jiménez, Antonio ; Lobato, Marı́a Fernández</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4572-a339d68fac587bfede198aa0a6963afeb1805e8342c238eb1603be19f4e17ad73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Chemistry, Physical - methods</topic><topic>dA, deoxyadenosine</topic><topic>Escherichia coli</topic><topic>Escherichia coli - genetics</topic><topic>Gene Deletion</topic><topic>Gene Expression</topic><topic>Genes, Bacterial</topic><topic>Kinetics</topic><topic>Molecular Structure</topic><topic>Multigene Family</topic><topic>ODMP, O-demethylpuromycin</topic><topic>ORF, open reading frame</topic><topic>Pac, puromycin N-acetyltransferase</topic><topic>Plasmids</topic><topic>pur cluster</topic><topic>pur6 gene</topic><topic>Puromycin - biosynthesis</topic><topic>Puromycin Aminonucleoside - chemistry</topic><topic>Puromycin Aminonucleoside - metabolism</topic><topic>puromycin aminonucleoside, N 6,N 6-dimethyl-3′-amino-3′-deoxyadenosine</topic><topic>Streptomyces</topic><topic>Streptomyces - genetics</topic><topic>Streptomyces - metabolism</topic><topic>Streptomyces alboniger</topic><topic>Streptomyces lividans</topic><topic>Streptomyces lividans - genetics</topic><topic>TDMP, tridemethylpuromycin</topic><topic>Tyrosinyl-aminonucleoside synthetase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ángel Rubio, Miguel</creatorcontrib><creatorcontrib>Barrado, Patricia</creatorcontrib><creatorcontrib>Carlos Espinosa, Juan</creatorcontrib><creatorcontrib>Jiménez, Antonio</creatorcontrib><creatorcontrib>Lobato, Marı́a Fernández</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ángel Rubio, Miguel</au><au>Barrado, Patricia</au><au>Carlos Espinosa, Juan</au><au>Jiménez, Antonio</au><au>Lobato, Marı́a Fernández</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The pur6 gene of the puromycin biosynthetic gene cluster from Streptomyces alboniger encodes a tyrosinyl-aminonucleoside synthetase</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2004-11-19</date><risdate>2004</risdate><volume>577</volume><issue>3</issue><spage>371</spage><epage>375</epage><pages>371-375</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>The
pur6 gene of the puromycin biosynthetic gene (
pur) cluster from
Streptomyces alboniger is shown to be essential for puromycin biosynthesis. Cell lysates from this mycelial bacterium were active in linking
l-tyrosine to both 3
′-amino-3
′-deoxyadenosine and
N
6,
N
6-dimethyl-3
′-amino-3
′-deoxyadenosine with a peptide-like bond. Identical reactions were performed by cell lysates from
Streptomyces lividans or
Escherichia coli transformants that expressed
pur6 from a variety of plasmid constructs. Physicochemical and biochemical analyses suggested that their products were tridemethyl puromycin and
O-demethylpuromycin, respectively. Therefore, it appears that Pur6 is the tyrosinyl-aminonucleoside synthetase of the puromycin biosynthetic pathway.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>15556612</pmid><doi>10.1016/j.febslet.2004.09.087</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2004-11, Vol.577 (3), p.371-375 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67098242 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; ScienceDirect Journals (5 years ago - present); Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Chemistry, Physical - methods dA, deoxyadenosine Escherichia coli Escherichia coli - genetics Gene Deletion Gene Expression Genes, Bacterial Kinetics Molecular Structure Multigene Family ODMP, O-demethylpuromycin ORF, open reading frame Pac, puromycin N-acetyltransferase Plasmids pur cluster pur6 gene Puromycin - biosynthesis Puromycin Aminonucleoside - chemistry Puromycin Aminonucleoside - metabolism puromycin aminonucleoside, N 6,N 6-dimethyl-3′-amino-3′-deoxyadenosine Streptomyces Streptomyces - genetics Streptomyces - metabolism Streptomyces alboniger Streptomyces lividans Streptomyces lividans - genetics TDMP, tridemethylpuromycin Tyrosinyl-aminonucleoside synthetase |
| title | The pur6 gene of the puromycin biosynthetic gene cluster from Streptomyces alboniger encodes a tyrosinyl-aminonucleoside synthetase |
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