Silicon–hydroxyapatite bioactive coatings (Si–HA) from diatomaceous earth and silica. Study of adhesion and proliferation of osteoblast-like cells
The aim of this study consisted on investigating the influence of silicon substituted hydroxyapatite (Si–HA) coatings over the human osteoblast-like cell line (SaOS-2) behaviour. Diatomaceous earth and silica, together with commercial hydroxyapatite were respectively the silicon and HA sources used...
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| Veröffentlicht in: | Journal of materials science. Materials in medicine 2009-05, Vol.20 (5), p.1131-1136 |
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| creator | López-Álvarez, M. Solla, E. L. González, P. Serra, J. León, B. Marques, A. P. Reis, R. L. |
| description | The aim of this study consisted on investigating the influence of silicon substituted hydroxyapatite (Si–HA) coatings over the human osteoblast-like cell line (SaOS-2) behaviour. Diatomaceous earth and silica, together with commercial hydroxyapatite were respectively the silicon and HA sources used to produce the Si–HA coatings. HA coatings with 0 wt% of silicon were used as control of the experiment. Pulsed laser deposition (PLD) was the selected technique to deposit the coatings. The Si–HA thin films were characterized by Fourier Transformed Infrared Spectroscopy (FTIR) demonstrating the efficient transfer of Si to the HA structure. The in vitro cell culture was established to assess the cell attachment, proliferation and osteoblastic activity respectively by, Scanning Electron Microscopy (SEM), DNA and alkaline phosphatase (ALP) quantification. The SEM analysis demonstrated a similar adhesion behaviour of the cells on the tested materials and the maintenance of the typical osteoblastic morphology along the time of culture. The Si–HA coatings did not evidence any type of cytotoxic behaviour when compared with HA coatings. Moreover, both the proliferation rate and osteoblastic activity results showed a slightly better performance on the Si–HA coatings from diatoms than on the Si–HA from silica. |
| doi_str_mv | 10.1007/s10856-008-3658-0 |
| format | Article |
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Study of adhesion and proliferation of osteoblast-like cells</title><source>MEDLINE</source><source>SpringerLink Journals - AutoHoldings</source><creator>López-Álvarez, M. ; Solla, E. L. ; González, P. ; Serra, J. ; León, B. ; Marques, A. P. ; Reis, R. L.</creator><creatorcontrib>López-Álvarez, M. ; Solla, E. L. ; González, P. ; Serra, J. ; León, B. ; Marques, A. P. ; Reis, R. L.</creatorcontrib><description>The aim of this study consisted on investigating the influence of silicon substituted hydroxyapatite (Si–HA) coatings over the human osteoblast-like cell line (SaOS-2) behaviour. Diatomaceous earth and silica, together with commercial hydroxyapatite were respectively the silicon and HA sources used to produce the Si–HA coatings. HA coatings with 0 wt% of silicon were used as control of the experiment. Pulsed laser deposition (PLD) was the selected technique to deposit the coatings. The Si–HA thin films were characterized by Fourier Transformed Infrared Spectroscopy (FTIR) demonstrating the efficient transfer of Si to the HA structure. The in vitro cell culture was established to assess the cell attachment, proliferation and osteoblastic activity respectively by, Scanning Electron Microscopy (SEM), DNA and alkaline phosphatase (ALP) quantification. The SEM analysis demonstrated a similar adhesion behaviour of the cells on the tested materials and the maintenance of the typical osteoblastic morphology along the time of culture. The Si–HA coatings did not evidence any type of cytotoxic behaviour when compared with HA coatings. Moreover, both the proliferation rate and osteoblastic activity results showed a slightly better performance on the Si–HA coatings from diatoms than on the Si–HA from silica.</description><identifier>ISSN: 0957-4530</identifier><identifier>EISSN: 1573-4838</identifier><identifier>DOI: 10.1007/s10856-008-3658-0</identifier><identifier>PMID: 19089599</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Alkaline Phosphatase - metabolism ; Bacillariophyceae ; Biomaterials ; Biomedical engineering ; Biomedical Engineering and Bioengineering ; Biomedical materials ; Biophysics ; Cell Adhesion ; Cell adhesion & migration ; Cell Line ; Cell Proliferation ; Ceramics ; Ceramics - chemistry ; Chemistry and Materials Science ; Coated Materials, Biocompatible - chemistry ; Composites ; Diatomaceous Earth - chemistry ; DNA - metabolism ; Durapatite - chemistry ; Durapatite - isolation & purification ; Glass ; Humans ; Lasers ; Materials Science ; Materials Testing ; Microscopy, Electron, Scanning ; Natural Materials ; Osteoblasts - cytology ; Osteoblasts - metabolism ; Osteogenesis ; Polymer Sciences ; Regenerative Medicine/Tissue Engineering ; Silica ; Silicon - chemistry ; Silicon - isolation & purification ; Silicon Dioxide - chemistry ; Spectroscopy, Fourier Transform Infrared ; Surfaces and Interfaces ; Thin film coatings ; Thin Films</subject><ispartof>Journal of materials science. 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L.</creatorcontrib><creatorcontrib>González, P.</creatorcontrib><creatorcontrib>Serra, J.</creatorcontrib><creatorcontrib>León, B.</creatorcontrib><creatorcontrib>Marques, A. P.</creatorcontrib><creatorcontrib>Reis, R. L.</creatorcontrib><title>Silicon–hydroxyapatite bioactive coatings (Si–HA) from diatomaceous earth and silica. Study of adhesion and proliferation of osteoblast-like cells</title><title>Journal of materials science. Materials in medicine</title><addtitle>J Mater Sci: Mater Med</addtitle><addtitle>J Mater Sci Mater Med</addtitle><description>The aim of this study consisted on investigating the influence of silicon substituted hydroxyapatite (Si–HA) coatings over the human osteoblast-like cell line (SaOS-2) behaviour. Diatomaceous earth and silica, together with commercial hydroxyapatite were respectively the silicon and HA sources used to produce the Si–HA coatings. HA coatings with 0 wt% of silicon were used as control of the experiment. Pulsed laser deposition (PLD) was the selected technique to deposit the coatings. The Si–HA thin films were characterized by Fourier Transformed Infrared Spectroscopy (FTIR) demonstrating the efficient transfer of Si to the HA structure. The in vitro cell culture was established to assess the cell attachment, proliferation and osteoblastic activity respectively by, Scanning Electron Microscopy (SEM), DNA and alkaline phosphatase (ALP) quantification. The SEM analysis demonstrated a similar adhesion behaviour of the cells on the tested materials and the maintenance of the typical osteoblastic morphology along the time of culture. The Si–HA coatings did not evidence any type of cytotoxic behaviour when compared with HA coatings. Moreover, both the proliferation rate and osteoblastic activity results showed a slightly better performance on the Si–HA coatings from diatoms than on the Si–HA from silica.</description><subject>Alkaline Phosphatase - metabolism</subject><subject>Bacillariophyceae</subject><subject>Biomaterials</subject><subject>Biomedical engineering</subject><subject>Biomedical Engineering and Bioengineering</subject><subject>Biomedical materials</subject><subject>Biophysics</subject><subject>Cell Adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell Line</subject><subject>Cell Proliferation</subject><subject>Ceramics</subject><subject>Ceramics - chemistry</subject><subject>Chemistry and Materials Science</subject><subject>Coated Materials, Biocompatible - chemistry</subject><subject>Composites</subject><subject>Diatomaceous Earth - chemistry</subject><subject>DNA - metabolism</subject><subject>Durapatite - chemistry</subject><subject>Durapatite - isolation & purification</subject><subject>Glass</subject><subject>Humans</subject><subject>Lasers</subject><subject>Materials Science</subject><subject>Materials Testing</subject><subject>Microscopy, Electron, Scanning</subject><subject>Natural Materials</subject><subject>Osteoblasts - cytology</subject><subject>Osteoblasts - metabolism</subject><subject>Osteogenesis</subject><subject>Polymer Sciences</subject><subject>Regenerative Medicine/Tissue Engineering</subject><subject>Silica</subject><subject>Silicon - chemistry</subject><subject>Silicon - isolation & purification</subject><subject>Silicon Dioxide - chemistry</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Surfaces and Interfaces</subject><subject>Thin film coatings</subject><subject>Thin Films</subject><issn>0957-4530</issn><issn>1573-4838</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFks-KFDEQxoMo7rj6AF4keBA99Frp_OnOcVnUFRY8jJ5DOknvZO3ujEl6cW6-g-AD-iSm7YEFQfZUUPWrr6qoD6HnBM4IQPM2EWi5qADaigreVvAAbQhvaMVa2j5EG5C8qRincIKepHQDAExy_hidEAmt5FJu0K-tH7wJ0-8fP3cHG8P3g97r7LPDnQ_aZH_rsAklM10n_HrrC3d5_gb3MYzYep3DqI0Lc8JOx7zDerI4LYr6DG_zbA849FjbnUs-TH-r-xgG37tYJEumVEPKLnSDTrka_NcyzQ1Deooe9XpI7tkxnqIv7999vrisrj59-HhxflUZLniutBWiM4RJx5sebNMBaSgnQlpmXC06K6zpoGEdB2YEs5IbQ4SjwFpBLGX0FL1adcta32aXshp9WjbQ03KVEg3IhlB5L0gF5ZK27b1gTWpBgfACvvwHvAlznMq1qq5J-dA6lqyQiSGl6Hq1j37U8aAIqMUDavWAKh5QiwcUlJ4XR-G5G5296zg-vQD1CqRSmq5dvJv8f9U_0Mu_yA</recordid><startdate>20090501</startdate><enddate>20090501</enddate><creator>López-Álvarez, M.</creator><creator>Solla, E. 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Materials in medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>López-Álvarez, M.</au><au>Solla, E. L.</au><au>González, P.</au><au>Serra, J.</au><au>León, B.</au><au>Marques, A. P.</au><au>Reis, R. L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silicon–hydroxyapatite bioactive coatings (Si–HA) from diatomaceous earth and silica. Study of adhesion and proliferation of osteoblast-like cells</atitle><jtitle>Journal of materials science. Materials in medicine</jtitle><stitle>J Mater Sci: Mater Med</stitle><addtitle>J Mater Sci Mater Med</addtitle><date>2009-05-01</date><risdate>2009</risdate><volume>20</volume><issue>5</issue><spage>1131</spage><epage>1136</epage><pages>1131-1136</pages><issn>0957-4530</issn><eissn>1573-4838</eissn><abstract>The aim of this study consisted on investigating the influence of silicon substituted hydroxyapatite (Si–HA) coatings over the human osteoblast-like cell line (SaOS-2) behaviour. Diatomaceous earth and silica, together with commercial hydroxyapatite were respectively the silicon and HA sources used to produce the Si–HA coatings. HA coatings with 0 wt% of silicon were used as control of the experiment. Pulsed laser deposition (PLD) was the selected technique to deposit the coatings. The Si–HA thin films were characterized by Fourier Transformed Infrared Spectroscopy (FTIR) demonstrating the efficient transfer of Si to the HA structure. The in vitro cell culture was established to assess the cell attachment, proliferation and osteoblastic activity respectively by, Scanning Electron Microscopy (SEM), DNA and alkaline phosphatase (ALP) quantification. The SEM analysis demonstrated a similar adhesion behaviour of the cells on the tested materials and the maintenance of the typical osteoblastic morphology along the time of culture. The Si–HA coatings did not evidence any type of cytotoxic behaviour when compared with HA coatings. Moreover, both the proliferation rate and osteoblastic activity results showed a slightly better performance on the Si–HA coatings from diatoms than on the Si–HA from silica.</abstract><cop>Boston</cop><pub>Springer US</pub><pmid>19089599</pmid><doi>10.1007/s10856-008-3658-0</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Alkaline Phosphatase - metabolism Bacillariophyceae Biomaterials Biomedical engineering Biomedical Engineering and Bioengineering Biomedical materials Biophysics Cell Adhesion Cell adhesion & migration Cell Line Cell Proliferation Ceramics Ceramics - chemistry Chemistry and Materials Science Coated Materials, Biocompatible - chemistry Composites Diatomaceous Earth - chemistry DNA - metabolism Durapatite - chemistry Durapatite - isolation & purification Glass Humans Lasers Materials Science Materials Testing Microscopy, Electron, Scanning Natural Materials Osteoblasts - cytology Osteoblasts - metabolism Osteogenesis Polymer Sciences Regenerative Medicine/Tissue Engineering Silica Silicon - chemistry Silicon - isolation & purification Silicon Dioxide - chemistry Spectroscopy, Fourier Transform Infrared Surfaces and Interfaces Thin film coatings Thin Films |
| title | Silicon–hydroxyapatite bioactive coatings (Si–HA) from diatomaceous earth and silica. Study of adhesion and proliferation of osteoblast-like cells |
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