Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells
As an example of the cost-effective large-scale generation of small-interfering RNA (siRNAs), we have created transgenic tobacco plants that produce siRNAs targeted to the mRNA of the non-structural protein NS1 from the influenza A virus subtype H1N1. We have investigated if these siRNAs, specifical...
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| Veröffentlicht in: | FEBS letters 2004-11, Vol.577 (3), p.345-350 |
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| container_issue | 3 |
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| container_title | FEBS letters |
| container_volume | 577 |
| creator | Zhou, Yuanxiang Chan, Jack H. Chan, Annie Y. Chak, Regina K.F. Wong, Elaine Y.L. Chye, Mee-Len Peiris, Joseph S.M. Poon, Leo L.M. Lam, Eric |
| description | As an example of the cost-effective large-scale generation of small-interfering RNA (siRNAs), we have created transgenic tobacco plants that produce siRNAs targeted to the mRNA of the non-structural protein NS1 from the influenza A virus subtype H1N1. We have investigated if these siRNAs, specifically targeted to the 5
′-portion of the
NS1 transcripts (
5mNS1), would suppress viral propagation in mammalian cells. Agroinfiltration of transgenic tobacco with an
Agrobacterium strain harboring a
5mNS1-expressing binary vector caused a reduction in
5mNS1 transcripts in the siRNA-accumulating transgenic plants. Further, H1N1 infection of siRNA-transfected mammalian cells resulted in significant suppression of viral replication. These results demonstrate that plant-derived siRNAs can inhibit viral propagation through RNA interference and could potentially be applied in control of viral-borne diseases. |
| doi_str_mv | 10.1016/j.febslet.2004.10.027 |
| format | Article |
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′-portion of the
NS1 transcripts (
5mNS1), would suppress viral propagation in mammalian cells. Agroinfiltration of transgenic tobacco with an
Agrobacterium strain harboring a
5mNS1-expressing binary vector caused a reduction in
5mNS1 transcripts in the siRNA-accumulating transgenic plants. Further, H1N1 infection of siRNA-transfected mammalian cells resulted in significant suppression of viral replication. These results demonstrate that plant-derived siRNAs can inhibit viral propagation through RNA interference and could potentially be applied in control of viral-borne diseases.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/j.febslet.2004.10.027</identifier><identifier>PMID: 15556607</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>Agrobacterium ; Animals ; Anti-viral ; Base Sequence ; Cell Line ; Codon ; Dogs ; EYFP, gene encoding the enhanced yellow fluorescent protein ; Gene encoding the non-structural protein NS1 ; Gene Targeting ; Hemagglutination ; Influenza A virus ; Influenza A virus - genetics ; Influenza virus ; Mammalian cells ; MDCK, Madin–Darby canine kidney ; Nicotiana - chemistry ; Nicotiana - genetics ; NS1, gene encoding the non-structural protein NS1 ; Orthomyxoviridae Infections ; Plants, Genetically Modified - chemistry ; PTGS, post-transcriptional gene silencing ; Rhizobium - genetics ; RNA Interference ; RNA, Messenger - metabolism ; RNA, Small Interfering - chemistry ; RNA, Small Interfering - genetics ; RNA, Small Interfering - metabolism ; RNAi, RNA interference ; siRNA, small-interfering RNA ; Small-interfering RNA ; Transgenic plant ; Virus Replication</subject><ispartof>FEBS letters, 2004-11, Vol.577 (3), p.345-350</ispartof><rights>2004</rights><rights>FEBS Letters 577 (2004) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c570X-26e1f7af190ddbc28a885d77ce5b6a9bd3b461e028bd77b73d377db4b2cd041e3</citedby><cites>FETCH-LOGICAL-c570X-26e1f7af190ddbc28a885d77ce5b6a9bd3b461e028bd77b73d377db4b2cd041e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.febslet.2004.10.027$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.febslet.2004.10.027$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,1416,1432,3548,27922,27923,45572,45573,45993,46407,46831</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15556607$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhou, Yuanxiang</creatorcontrib><creatorcontrib>Chan, Jack H.</creatorcontrib><creatorcontrib>Chan, Annie Y.</creatorcontrib><creatorcontrib>Chak, Regina K.F.</creatorcontrib><creatorcontrib>Wong, Elaine Y.L.</creatorcontrib><creatorcontrib>Chye, Mee-Len</creatorcontrib><creatorcontrib>Peiris, Joseph S.M.</creatorcontrib><creatorcontrib>Poon, Leo L.M.</creatorcontrib><creatorcontrib>Lam, Eric</creatorcontrib><title>Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>As an example of the cost-effective large-scale generation of small-interfering RNA (siRNAs), we have created transgenic tobacco plants that produce siRNAs targeted to the mRNA of the non-structural protein NS1 from the influenza A virus subtype H1N1. We have investigated if these siRNAs, specifically targeted to the 5
′-portion of the
NS1 transcripts (
5mNS1), would suppress viral propagation in mammalian cells. Agroinfiltration of transgenic tobacco with an
Agrobacterium strain harboring a
5mNS1-expressing binary vector caused a reduction in
5mNS1 transcripts in the siRNA-accumulating transgenic plants. Further, H1N1 infection of siRNA-transfected mammalian cells resulted in significant suppression of viral replication. These results demonstrate that plant-derived siRNAs can inhibit viral propagation through RNA interference and could potentially be applied in control of viral-borne diseases.</description><subject>Agrobacterium</subject><subject>Animals</subject><subject>Anti-viral</subject><subject>Base Sequence</subject><subject>Cell Line</subject><subject>Codon</subject><subject>Dogs</subject><subject>EYFP, gene encoding the enhanced yellow fluorescent protein</subject><subject>Gene encoding the non-structural protein NS1</subject><subject>Gene Targeting</subject><subject>Hemagglutination</subject><subject>Influenza A virus</subject><subject>Influenza A virus - genetics</subject><subject>Influenza virus</subject><subject>Mammalian cells</subject><subject>MDCK, Madin–Darby canine kidney</subject><subject>Nicotiana - chemistry</subject><subject>Nicotiana - genetics</subject><subject>NS1, gene encoding the non-structural protein NS1</subject><subject>Orthomyxoviridae Infections</subject><subject>Plants, Genetically Modified - chemistry</subject><subject>PTGS, post-transcriptional gene silencing</subject><subject>Rhizobium - genetics</subject><subject>RNA Interference</subject><subject>RNA, Messenger - metabolism</subject><subject>RNA, Small Interfering - chemistry</subject><subject>RNA, Small Interfering - genetics</subject><subject>RNA, Small Interfering - metabolism</subject><subject>RNAi, RNA interference</subject><subject>siRNA, small-interfering RNA</subject><subject>Small-interfering RNA</subject><subject>Transgenic plant</subject><subject>Virus Replication</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUtv1DAUhS0EokPhJ4C8Ypep7cSPrFCpWopUgQRF6grLj5vKo8QJdjJV-fU4mpG6LCvrXp1zfHQ_hN5TsqWEirPdtgObe5i3jJCm7LaEyRdoQ5Wsq7oR6iXaEEKbisu2PkFvct6RMivavkYnlHMuBJEb9Ps2mZjvIQaHp97EufKQwh48zuHHt_OMnYk4L9OUIGc8pXEy92YOY8Rjh0Ps-gXiX4P3IS25zHgww2D6UEwO-j6_Ra8602d4d3xP0a-ry9uL6-rm-5evF-c3leOS3FVMAO2k6WhLvLeOKaMU91I64FaY1vraNoICYcqWrZW1r6X0trHMedJQqE_Rx0NuafhngTzrIeS1gYkwLlkLSVrOuHpWSFslWVuLIuQHoUtjzgk6PaUwmPSoKdErAb3TRwJ6JbCuC4Hi-3D8YLED-CfX8eRFcH0QPIQeHv8vVV9dfmY_V5wrTdIQygS9K1GfDlFQTrsPkHR2AaIDHxK4WfsxPNP2H4TCsS4</recordid><startdate>20041119</startdate><enddate>20041119</enddate><creator>Zhou, Yuanxiang</creator><creator>Chan, Jack H.</creator><creator>Chan, Annie Y.</creator><creator>Chak, Regina K.F.</creator><creator>Wong, Elaine Y.L.</creator><creator>Chye, Mee-Len</creator><creator>Peiris, Joseph S.M.</creator><creator>Poon, Leo L.M.</creator><creator>Lam, Eric</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20041119</creationdate><title>Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells</title><author>Zhou, Yuanxiang ; Chan, Jack H. ; Chan, Annie Y. ; Chak, Regina K.F. ; Wong, Elaine Y.L. ; Chye, Mee-Len ; Peiris, Joseph S.M. ; Poon, Leo L.M. ; Lam, Eric</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c570X-26e1f7af190ddbc28a885d77ce5b6a9bd3b461e028bd77b73d377db4b2cd041e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Agrobacterium</topic><topic>Animals</topic><topic>Anti-viral</topic><topic>Base Sequence</topic><topic>Cell Line</topic><topic>Codon</topic><topic>Dogs</topic><topic>EYFP, gene encoding the enhanced yellow fluorescent protein</topic><topic>Gene encoding the non-structural protein NS1</topic><topic>Gene Targeting</topic><topic>Hemagglutination</topic><topic>Influenza A virus</topic><topic>Influenza A virus - genetics</topic><topic>Influenza virus</topic><topic>Mammalian cells</topic><topic>MDCK, Madin–Darby canine kidney</topic><topic>Nicotiana - chemistry</topic><topic>Nicotiana - genetics</topic><topic>NS1, gene encoding the non-structural protein NS1</topic><topic>Orthomyxoviridae Infections</topic><topic>Plants, Genetically Modified - chemistry</topic><topic>PTGS, post-transcriptional gene silencing</topic><topic>Rhizobium - genetics</topic><topic>RNA Interference</topic><topic>RNA, Messenger - metabolism</topic><topic>RNA, Small Interfering - chemistry</topic><topic>RNA, Small Interfering - genetics</topic><topic>RNA, Small Interfering - metabolism</topic><topic>RNAi, RNA interference</topic><topic>siRNA, small-interfering RNA</topic><topic>Small-interfering RNA</topic><topic>Transgenic plant</topic><topic>Virus Replication</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhou, Yuanxiang</creatorcontrib><creatorcontrib>Chan, Jack H.</creatorcontrib><creatorcontrib>Chan, Annie Y.</creatorcontrib><creatorcontrib>Chak, Regina K.F.</creatorcontrib><creatorcontrib>Wong, Elaine Y.L.</creatorcontrib><creatorcontrib>Chye, Mee-Len</creatorcontrib><creatorcontrib>Peiris, Joseph S.M.</creatorcontrib><creatorcontrib>Poon, Leo L.M.</creatorcontrib><creatorcontrib>Lam, Eric</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhou, Yuanxiang</au><au>Chan, Jack H.</au><au>Chan, Annie Y.</au><au>Chak, Regina K.F.</au><au>Wong, Elaine Y.L.</au><au>Chye, Mee-Len</au><au>Peiris, Joseph S.M.</au><au>Poon, Leo L.M.</au><au>Lam, Eric</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>2004-11-19</date><risdate>2004</risdate><volume>577</volume><issue>3</issue><spage>345</spage><epage>350</epage><pages>345-350</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>As an example of the cost-effective large-scale generation of small-interfering RNA (siRNAs), we have created transgenic tobacco plants that produce siRNAs targeted to the mRNA of the non-structural protein NS1 from the influenza A virus subtype H1N1. We have investigated if these siRNAs, specifically targeted to the 5
′-portion of the
NS1 transcripts (
5mNS1), would suppress viral propagation in mammalian cells. Agroinfiltration of transgenic tobacco with an
Agrobacterium strain harboring a
5mNS1-expressing binary vector caused a reduction in
5mNS1 transcripts in the siRNA-accumulating transgenic plants. Further, H1N1 infection of siRNA-transfected mammalian cells resulted in significant suppression of viral replication. These results demonstrate that plant-derived siRNAs can inhibit viral propagation through RNA interference and could potentially be applied in control of viral-borne diseases.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>15556607</pmid><doi>10.1016/j.febslet.2004.10.027</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 2004-11, Vol.577 (3), p.345-350 |
| issn | 0014-5793 1873-3468 |
| language | eng |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Wiley Free Content; ScienceDirect Journals (5 years ago - present); Wiley Online Library All Journals; Alma/SFX Local Collection |
| subjects | Agrobacterium Animals Anti-viral Base Sequence Cell Line Codon Dogs EYFP, gene encoding the enhanced yellow fluorescent protein Gene encoding the non-structural protein NS1 Gene Targeting Hemagglutination Influenza A virus Influenza A virus - genetics Influenza virus Mammalian cells MDCK, Madin–Darby canine kidney Nicotiana - chemistry Nicotiana - genetics NS1, gene encoding the non-structural protein NS1 Orthomyxoviridae Infections Plants, Genetically Modified - chemistry PTGS, post-transcriptional gene silencing Rhizobium - genetics RNA Interference RNA, Messenger - metabolism RNA, Small Interfering - chemistry RNA, Small Interfering - genetics RNA, Small Interfering - metabolism RNAi, RNA interference siRNA, small-interfering RNA Small-interfering RNA Transgenic plant Virus Replication |
| title | Transgenic plant-derived siRNAs can suppress propagation of influenza virus in mammalian cells |
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