Optimal Conditions for Successful In Vitro Fertilization and Subsequent Embryonic Development in Sprague-Dawley Rats
The present study was conducted to determine the optimal conditions for successful in vitro fertilization (IVF) in Sprague-Dawley (SD) rats. The IVF of oocytes from SD and Wistar rats was compared in different fertilization media (mR1ECM, IVF-20, and modified Krebs-Ringer bicarbonate solution [mKRB]...
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| Veröffentlicht in: | Biology of reproduction 2004-12, Vol.71 (6), p.1974-1979 |
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| container_end_page | 1979 |
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| container_issue | 6 |
| container_start_page | 1974 |
| container_title | Biology of reproduction |
| container_volume | 71 |
| creator | JIANG, Jin-Yi TSANG, Benjamin K |
| description | The present study was conducted to determine the optimal conditions for successful in vitro fertilization (IVF) in Sprague-Dawley
(SD) rats. The IVF of oocytes from SD and Wistar rats was compared in different fertilization media (mR1ECM, IVF-20, and modified
Krebs-Ringer bicarbonate solution [mKRB]), and IVF conditions were then optimized for oocytes of the SD strain. Results showed
that in mR1ECM medium, fertilization rates were markedly lower in SD rats (15%) than in the Wistar strain (73%), although
this response was significantly improved by increasing the NaCl concentration. In addition, fertilization rates in SD rats
were higher in modified IVF-20 (73%) than in IVF-20 (18%) and mKRB (53%). In contrast, fertilization rates in Wistar rats
were higher in IVF-20 and modified IVF-20 than in mKRB (78%, 74%, and 36%, respectively). Further investigation concerning
the effects of the NaCl supplementation (10â 40 mM) in IVF-20 on the fertilization of oocytes in the SD strain indicated that
significantly higher percentages of oocytes were fertilized in IVF-20 supplemented with 30 mM NaCl (66%) and developed to
the blastocyst stage (47%) in vitro. After transfer, embryos derived from this IVF system developed to term at a percentage
comparable to that of in vivo-fertilized controls. In conclusion, differences exist in optimal IVF conditions between rat
strains, and a modified culture medium has been successfully developed for assessment of the developmental competence of oocytes
in SD rats. |
| doi_str_mv | 10.1095/biolreprod.104.032839 |
| format | Article |
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(SD) rats. The IVF of oocytes from SD and Wistar rats was compared in different fertilization media (mR1ECM, IVF-20, and modified
Krebs-Ringer bicarbonate solution [mKRB]), and IVF conditions were then optimized for oocytes of the SD strain. Results showed
that in mR1ECM medium, fertilization rates were markedly lower in SD rats (15%) than in the Wistar strain (73%), although
this response was significantly improved by increasing the NaCl concentration. In addition, fertilization rates in SD rats
were higher in modified IVF-20 (73%) than in IVF-20 (18%) and mKRB (53%). In contrast, fertilization rates in Wistar rats
were higher in IVF-20 and modified IVF-20 than in mKRB (78%, 74%, and 36%, respectively). Further investigation concerning
the effects of the NaCl supplementation (10â 40 mM) in IVF-20 on the fertilization of oocytes in the SD strain indicated that
significantly higher percentages of oocytes were fertilized in IVF-20 supplemented with 30 mM NaCl (66%) and developed to
the blastocyst stage (47%) in vitro. After transfer, embryos derived from this IVF system developed to term at a percentage
comparable to that of in vivo-fertilized controls. In conclusion, differences exist in optimal IVF conditions between rat
strains, and a modified culture medium has been successfully developed for assessment of the developmental competence of oocytes
in SD rats.</description><identifier>ISSN: 0006-3363</identifier><identifier>EISSN: 1529-7268</identifier><identifier>DOI: 10.1095/biolreprod.104.032839</identifier><identifier>PMID: 15317689</identifier><identifier>CODEN: BIREBV</identifier><language>eng</language><publisher>Madison, WI: Society for the Study of Reproduction</publisher><subject>Animals ; Biological and medical sciences ; Culture Media - pharmacology ; Early stages. Segmentation. Gastrulation. Neurulation ; Embryo Culture Techniques ; Embryology: invertebrates and vertebrates. Teratology ; Female ; Fertility ; Fertilization in Vitro ; Fundamental and applied biological sciences. Psychology ; Isotonic Solutions - pharmacology ; Male ; Oocytes - drug effects ; Osmolar Concentration ; Rats ; Rats, Sprague-Dawley - embryology ; Rats, Sprague-Dawley - physiology ; Rats, Wistar ; Sodium Chloride - pharmacology ; Species Specificity ; Vertebrates: reproduction</subject><ispartof>Biology of reproduction, 2004-12, Vol.71 (6), p.1974-1979</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16305484$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15317689$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>JIANG, Jin-Yi</creatorcontrib><creatorcontrib>TSANG, Benjamin K</creatorcontrib><title>Optimal Conditions for Successful In Vitro Fertilization and Subsequent Embryonic Development in Sprague-Dawley Rats</title><title>Biology of reproduction</title><addtitle>Biol Reprod</addtitle><description>The present study was conducted to determine the optimal conditions for successful in vitro fertilization (IVF) in Sprague-Dawley
(SD) rats. The IVF of oocytes from SD and Wistar rats was compared in different fertilization media (mR1ECM, IVF-20, and modified
Krebs-Ringer bicarbonate solution [mKRB]), and IVF conditions were then optimized for oocytes of the SD strain. Results showed
that in mR1ECM medium, fertilization rates were markedly lower in SD rats (15%) than in the Wistar strain (73%), although
this response was significantly improved by increasing the NaCl concentration. In addition, fertilization rates in SD rats
were higher in modified IVF-20 (73%) than in IVF-20 (18%) and mKRB (53%). In contrast, fertilization rates in Wistar rats
were higher in IVF-20 and modified IVF-20 than in mKRB (78%, 74%, and 36%, respectively). Further investigation concerning
the effects of the NaCl supplementation (10â 40 mM) in IVF-20 on the fertilization of oocytes in the SD strain indicated that
significantly higher percentages of oocytes were fertilized in IVF-20 supplemented with 30 mM NaCl (66%) and developed to
the blastocyst stage (47%) in vitro. After transfer, embryos derived from this IVF system developed to term at a percentage
comparable to that of in vivo-fertilized controls. In conclusion, differences exist in optimal IVF conditions between rat
strains, and a modified culture medium has been successfully developed for assessment of the developmental competence of oocytes
in SD rats.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Culture Media - pharmacology</subject><subject>Early stages. Segmentation. Gastrulation. Neurulation</subject><subject>Embryo Culture Techniques</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>Fertility</subject><subject>Fertilization in Vitro</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isotonic Solutions - pharmacology</subject><subject>Male</subject><subject>Oocytes - drug effects</subject><subject>Osmolar Concentration</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley - embryology</subject><subject>Rats, Sprague-Dawley - physiology</subject><subject>Rats, Wistar</subject><subject>Sodium Chloride - pharmacology</subject><subject>Species Specificity</subject><subject>Vertebrates: reproduction</subject><issn>0006-3363</issn><issn>1529-7268</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpFkMFu1DAQhi1URJfCI1D50t5S7Nix4yPabaFSpUoUuEaOM-m6cuxgO42Wp8cVi3oazejT_48-hD5RckWJaj73NrgIcwxD2fkVYXXL1Bu0oU2tKlmL9gRtCCGiYkywU_Q-pSdCKGc1e4dOacOoFK3aoHw_Zztph7fBDzbb4BMeQ8QPizGQ0rg4fOvxL5tjwDcQs3X2j37BsPZDofoEvxfwGV9PfTwEbw3ewTO4ME8vV-vxwxz14wLVTq8ODvi7zukDejtql-DjcZ6hnzfXP7bfqrv7r7fbL3fVvvyfq5ENjAOjwHsJLbSNGg0j0EqiFAEFYCRhhJO6aRrZGzkMLeF1zYVQouajYmfo8l9u0VS-TLmbbDLgnPYQltSJksSp5AU8P4JLP8HQzbE4iYfuv6cCXBwBnYx2Y9Te2PTKCUYa3vLXxr193K82QpeKXFdiWbeuq6Sd6KgqjX8BHJWIOw</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>JIANG, Jin-Yi</creator><creator>TSANG, Benjamin K</creator><general>Society for the Study of Reproduction</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>20041201</creationdate><title>Optimal Conditions for Successful In Vitro Fertilization and Subsequent Embryonic Development in Sprague-Dawley Rats</title><author>JIANG, Jin-Yi ; TSANG, Benjamin K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h268t-f3d34e31e4b7e8e859fc30e870990e9eec70304025557bc7dd804224669624f93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Culture Media - pharmacology</topic><topic>Early stages. Segmentation. Gastrulation. Neurulation</topic><topic>Embryo Culture Techniques</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>Fertility</topic><topic>Fertilization in Vitro</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isotonic Solutions - pharmacology</topic><topic>Male</topic><topic>Oocytes - drug effects</topic><topic>Osmolar Concentration</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley - embryology</topic><topic>Rats, Sprague-Dawley - physiology</topic><topic>Rats, Wistar</topic><topic>Sodium Chloride - pharmacology</topic><topic>Species Specificity</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JIANG, Jin-Yi</creatorcontrib><creatorcontrib>TSANG, Benjamin K</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Biology of reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JIANG, Jin-Yi</au><au>TSANG, Benjamin K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Optimal Conditions for Successful In Vitro Fertilization and Subsequent Embryonic Development in Sprague-Dawley Rats</atitle><jtitle>Biology of reproduction</jtitle><addtitle>Biol Reprod</addtitle><date>2004-12-01</date><risdate>2004</risdate><volume>71</volume><issue>6</issue><spage>1974</spage><epage>1979</epage><pages>1974-1979</pages><issn>0006-3363</issn><eissn>1529-7268</eissn><coden>BIREBV</coden><abstract>The present study was conducted to determine the optimal conditions for successful in vitro fertilization (IVF) in Sprague-Dawley
(SD) rats. The IVF of oocytes from SD and Wistar rats was compared in different fertilization media (mR1ECM, IVF-20, and modified
Krebs-Ringer bicarbonate solution [mKRB]), and IVF conditions were then optimized for oocytes of the SD strain. Results showed
that in mR1ECM medium, fertilization rates were markedly lower in SD rats (15%) than in the Wistar strain (73%), although
this response was significantly improved by increasing the NaCl concentration. In addition, fertilization rates in SD rats
were higher in modified IVF-20 (73%) than in IVF-20 (18%) and mKRB (53%). In contrast, fertilization rates in Wistar rats
were higher in IVF-20 and modified IVF-20 than in mKRB (78%, 74%, and 36%, respectively). Further investigation concerning
the effects of the NaCl supplementation (10â 40 mM) in IVF-20 on the fertilization of oocytes in the SD strain indicated that
significantly higher percentages of oocytes were fertilized in IVF-20 supplemented with 30 mM NaCl (66%) and developed to
the blastocyst stage (47%) in vitro. After transfer, embryos derived from this IVF system developed to term at a percentage
comparable to that of in vivo-fertilized controls. In conclusion, differences exist in optimal IVF conditions between rat
strains, and a modified culture medium has been successfully developed for assessment of the developmental competence of oocytes
in SD rats.</abstract><cop>Madison, WI</cop><pub>Society for the Study of Reproduction</pub><pmid>15317689</pmid><doi>10.1095/biolreprod.104.032839</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; BioOne Complete; Oxford University Press Journals All Titles (1996-Current); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological and medical sciences Culture Media - pharmacology Early stages. Segmentation. Gastrulation. Neurulation Embryo Culture Techniques Embryology: invertebrates and vertebrates. Teratology Female Fertility Fertilization in Vitro Fundamental and applied biological sciences. Psychology Isotonic Solutions - pharmacology Male Oocytes - drug effects Osmolar Concentration Rats Rats, Sprague-Dawley - embryology Rats, Sprague-Dawley - physiology Rats, Wistar Sodium Chloride - pharmacology Species Specificity Vertebrates: reproduction |
| title | Optimal Conditions for Successful In Vitro Fertilization and Subsequent Embryonic Development in Sprague-Dawley Rats |
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