Extracellular pressure stimulates colon cancer cell adhesion in vitro and to surgical wounds by Src (sarcoma protein) activation
We hypothesized that pressure stimulates colon cancer cell adhesion to surgical wounds. We quantitated adhesion of murine 26/51 transplantable colon cancer cells by cell counting or chromium 51–labeling. Tumor cells were added to murine surgical wounds after 30 minutes preincubation under ambient or...
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| Veröffentlicht in: | The American journal of surgery 2004-11, Vol.188 (5), p.467-473 |
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| creator | van der Voort van Zyp, Jochem Thamilselvan, Vijayalakshmi Walsh, Mary Polin, Lisa Basson, Marc D. |
| description | We hypothesized that pressure stimulates colon cancer cell adhesion to surgical wounds.
We quantitated adhesion of murine 26/51 transplantable colon cancer cells by cell counting or chromium 51–labeling. Tumor cells were added to murine surgical wounds after 30 minutes preincubation under ambient or 15 mm Hg increased pressure. Src activation was assayed by immunoblotting for phosphorylated Src and inhibited by 4-amino-5-(4chlorophenyl)-7-(t-butyl)pyrazolo-[3-4-d]pyrimidine (PP2).
Pressure stimulated colon 26/51 cell adhesion to murine wounds by 43% to 52% (n = 9,
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| doi_str_mv | 10.1016/j.amjsurg.2004.07.005 |
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We quantitated adhesion of murine 26/51 transplantable colon cancer cells by cell counting or chromium 51–labeling. Tumor cells were added to murine surgical wounds after 30 minutes preincubation under ambient or 15 mm Hg increased pressure. Src activation was assayed by immunoblotting for phosphorylated Src and inhibited by 4-amino-5-(4chlorophenyl)-7-(t-butyl)pyrazolo-[3-4-d]pyrimidine (PP2).
Pressure stimulated colon 26/51 cell adhesion to murine wounds by 43% to 52% (n = 9,
P <0.05 each). Pressure stimulated Src phosphorylation by 39% ± 4% (n = 5,
P = 0.004) in colon 26 cells. The Src inhibitor PP2 (20 μmol/L) did not inhibit Src phosphorylation at ambient pressure but prevented pressure stimulation of Src phosphorylation. Src blockade by PP2 did not affect basal adhesion of either tumor to murine wounds but completely blocked pressure stimulation of adhesion (n = 4,
P <0.001 each).
Increased pressure may activate cancer adhesion to surgical wounds via Src. Src antagonists might inhibit this process.</description><identifier>ISSN: 0002-9610</identifier><identifier>EISSN: 1879-1883</identifier><identifier>DOI: 10.1016/j.amjsurg.2004.07.005</identifier><identifier>PMID: 15546552</identifier><identifier>CODEN: AJSUAB</identifier><language>eng</language><publisher>New York, NY: Elsevier Inc</publisher><subject>Animals ; Biological and medical sciences ; Blotting, Western ; Calcineurin - pharmacology ; Cell adhesion & migration ; Cell Adhesion - drug effects ; Cell Adhesion - physiology ; Cell signaling ; Colon ; Colonic Neoplasms - drug therapy ; Colonic Neoplasms - pathology ; Colorectal cancer ; Disease Models, Animal ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; General aspects ; Male ; Medical sciences ; Mice ; Mice, Inbred BALB C ; Mice, Inbred C57BL ; Mouse ; Neoplasm Seeding ; Neoplasm Transplantation ; Pressure ; Probability ; Proteins ; Proto-Oncogene Proteins pp60(c-src) - drug effects ; Proto-Oncogene Proteins pp60(c-src) - physiology ; Rodents ; Sensitivity and Specificity ; Signal Transduction - drug effects ; Signal Transduction - physiology ; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus ; Transplantable tumors ; Tumor Cells, Cultured ; Tumors ; Wound implantation</subject><ispartof>The American journal of surgery, 2004-11, Vol.188 (5), p.467-473</ispartof><rights>2004</rights><rights>2005 INIST-CNRS</rights><rights>Copyright Elsevier Limited Nov 2004</rights><rights>Copyright Elsevier Limited Jan 2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c447t-b2112cb3742d1628125309f7ef32f45ced9faf67ef5b2bc5dcebc7a75662151e3</citedby><cites>FETCH-LOGICAL-c447t-b2112cb3742d1628125309f7ef32f45ced9faf67ef5b2bc5dcebc7a75662151e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/1444598127?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>310,311,315,782,786,791,792,3552,23937,23938,25147,27931,27932,46002,64392,64394,64396,72476</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16310655$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15546552$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>van der Voort van Zyp, Jochem</creatorcontrib><creatorcontrib>Thamilselvan, Vijayalakshmi</creatorcontrib><creatorcontrib>Walsh, Mary</creatorcontrib><creatorcontrib>Polin, Lisa</creatorcontrib><creatorcontrib>Basson, Marc D.</creatorcontrib><title>Extracellular pressure stimulates colon cancer cell adhesion in vitro and to surgical wounds by Src (sarcoma protein) activation</title><title>The American journal of surgery</title><addtitle>Am J Surg</addtitle><description>We hypothesized that pressure stimulates colon cancer cell adhesion to surgical wounds.
We quantitated adhesion of murine 26/51 transplantable colon cancer cells by cell counting or chromium 51–labeling. Tumor cells were added to murine surgical wounds after 30 minutes preincubation under ambient or 15 mm Hg increased pressure. Src activation was assayed by immunoblotting for phosphorylated Src and inhibited by 4-amino-5-(4chlorophenyl)-7-(t-butyl)pyrazolo-[3-4-d]pyrimidine (PP2).
Pressure stimulated colon 26/51 cell adhesion to murine wounds by 43% to 52% (n = 9,
P <0.05 each). Pressure stimulated Src phosphorylation by 39% ± 4% (n = 5,
P = 0.004) in colon 26 cells. The Src inhibitor PP2 (20 μmol/L) did not inhibit Src phosphorylation at ambient pressure but prevented pressure stimulation of Src phosphorylation. Src blockade by PP2 did not affect basal adhesion of either tumor to murine wounds but completely blocked pressure stimulation of adhesion (n = 4,
P <0.001 each).
Increased pressure may activate cancer adhesion to surgical wounds via Src. Src antagonists might inhibit this process.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Calcineurin - pharmacology</subject><subject>Cell adhesion & migration</subject><subject>Cell Adhesion - drug effects</subject><subject>Cell Adhesion - physiology</subject><subject>Cell signaling</subject><subject>Colon</subject><subject>Colonic Neoplasms - drug therapy</subject><subject>Colonic Neoplasms - pathology</subject><subject>Colorectal cancer</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>General aspects</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred C57BL</subject><subject>Mouse</subject><subject>Neoplasm Seeding</subject><subject>Neoplasm Transplantation</subject><subject>Pressure</subject><subject>Probability</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins pp60(c-src) - drug effects</subject><subject>Proto-Oncogene Proteins pp60(c-src) - physiology</subject><subject>Rodents</subject><subject>Sensitivity and Specificity</subject><subject>Signal Transduction - drug effects</subject><subject>Signal Transduction - physiology</subject><subject>Stomach. Duodenum. Small intestine. Colon. Rectum. Anus</subject><subject>Transplantable tumors</subject><subject>Tumor Cells, Cultured</subject><subject>Tumors</subject><subject>Wound implantation</subject><issn>0002-9610</issn><issn>1879-1883</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>8G5</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>GUQSH</sourceid><sourceid>M2O</sourceid><recordid>eNqFkc2L1jAQxoso7uvqn6AERNFDa5Lmoz0tsqwfsOBBPYc0nWpK26xJ-ure_NOd8hYWPOgpzPCbJ8_MUxRPGa0YZerNWNl5TGv8VnFKRUV1Ram8VxxYo9uSNU19vzhQSnnZKkbPikcpjVgyJuqHxRmTUigp-aH4ffUrR-tgmtbJRnITIaEokJT9jJ0MibgwhYU4uziIZCOJ7b9D8tj0Czn6HAOxS09yIJsf7-xEfoZ16RPpbsnn6MirZKMLs0X5kMEvr4l12R9tRo3HxYPBTgme7O958fXd1ZfLD-X1p_cfL99el04IncuOM8ZdV2vBe6Z4w7isaTtoGGo-COmgbwc7KKxlxzsnewed01ZLpTiTDOrz4uVJFz38WCFlM_u0bWMXCGsyStOmVQ1D8Plf4BjWuKA3w4QQssW_9T8pWjNOa64VUvJEuRhSijCYm-hnG28RMluMZjR7jGaL0VBtMEace7arr90M_d3UnhsCL3bAJrz3EDEdn-44VTOKIHIXJw7wtEcP0STnAZPsfQSXTR_8f6z8AZNMvwE</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>van der Voort van Zyp, Jochem</creator><creator>Thamilselvan, Vijayalakshmi</creator><creator>Walsh, Mary</creator><creator>Polin, Lisa</creator><creator>Basson, Marc D.</creator><general>Elsevier Inc</general><general>Elsevier</general><general>Elsevier Limited</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Extracellular pressure stimulates colon cancer cell adhesion in vitro and to surgical wounds by Src (sarcoma protein) activation</title><author>van der Voort van Zyp, Jochem ; Thamilselvan, Vijayalakshmi ; Walsh, Mary ; Polin, Lisa ; Basson, Marc D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c447t-b2112cb3742d1628125309f7ef32f45ced9faf67ef5b2bc5dcebc7a75662151e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Calcineurin - pharmacology</topic><topic>Cell adhesion & migration</topic><topic>Cell Adhesion - drug effects</topic><topic>Cell Adhesion - physiology</topic><topic>Cell signaling</topic><topic>Colon</topic><topic>Colonic Neoplasms - drug therapy</topic><topic>Colonic Neoplasms - pathology</topic><topic>Colorectal cancer</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>General aspects</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred C57BL</topic><topic>Mouse</topic><topic>Neoplasm Seeding</topic><topic>Neoplasm Transplantation</topic><topic>Pressure</topic><topic>Probability</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins pp60(c-src) - drug effects</topic><topic>Proto-Oncogene Proteins pp60(c-src) - physiology</topic><topic>Rodents</topic><topic>Sensitivity and Specificity</topic><topic>Signal Transduction - drug effects</topic><topic>Signal Transduction - physiology</topic><topic>Stomach. Duodenum. Small intestine. Colon. Rectum. 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We quantitated adhesion of murine 26/51 transplantable colon cancer cells by cell counting or chromium 51–labeling. Tumor cells were added to murine surgical wounds after 30 minutes preincubation under ambient or 15 mm Hg increased pressure. Src activation was assayed by immunoblotting for phosphorylated Src and inhibited by 4-amino-5-(4chlorophenyl)-7-(t-butyl)pyrazolo-[3-4-d]pyrimidine (PP2).
Pressure stimulated colon 26/51 cell adhesion to murine wounds by 43% to 52% (n = 9,
P <0.05 each). Pressure stimulated Src phosphorylation by 39% ± 4% (n = 5,
P = 0.004) in colon 26 cells. The Src inhibitor PP2 (20 μmol/L) did not inhibit Src phosphorylation at ambient pressure but prevented pressure stimulation of Src phosphorylation. Src blockade by PP2 did not affect basal adhesion of either tumor to murine wounds but completely blocked pressure stimulation of adhesion (n = 4,
P <0.001 each).
Increased pressure may activate cancer adhesion to surgical wounds via Src. Src antagonists might inhibit this process.</abstract><cop>New York, NY</cop><pub>Elsevier Inc</pub><pmid>15546552</pmid><doi>10.1016/j.amjsurg.2004.07.005</doi><tpages>7</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Blotting, Western Calcineurin - pharmacology Cell adhesion & migration Cell Adhesion - drug effects Cell Adhesion - physiology Cell signaling Colon Colonic Neoplasms - drug therapy Colonic Neoplasms - pathology Colorectal cancer Disease Models, Animal Female Gastroenterology. Liver. Pancreas. Abdomen General aspects Male Medical sciences Mice Mice, Inbred BALB C Mice, Inbred C57BL Mouse Neoplasm Seeding Neoplasm Transplantation Pressure Probability Proteins Proto-Oncogene Proteins pp60(c-src) - drug effects Proto-Oncogene Proteins pp60(c-src) - physiology Rodents Sensitivity and Specificity Signal Transduction - drug effects Signal Transduction - physiology Stomach. Duodenum. Small intestine. Colon. Rectum. Anus Transplantable tumors Tumor Cells, Cultured Tumors Wound implantation |
| title | Extracellular pressure stimulates colon cancer cell adhesion in vitro and to surgical wounds by Src (sarcoma protein) activation |
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