Temporal and spatial characterization of cellular constituents during neointimal hyperplasia after vascular injury: Potential contribution of bone-marrow-derived progenitors to arterial remodeling
Exuberant smooth muscle cells (SMCs) hyperplasia is the major cause of postangioplasty restenosis. We suggested that circulating smooth muscle progenitor cells might contribute to lesion formation after vascular injury. We extensively investigated the cellular constituents during neointimal formatio...
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| Veröffentlicht in: | Cardiovascular pathology 2004-11, Vol.13 (6), p.306-312 |
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| creator | Shoji, Makoto Sata, Masataka Fukuda, Daiju Tanaka, Kimie Sato, Takatoshi Iso, Yoshitaka Shibata, Masayuki Suzuki, Hiroshi Koba, Shinji Geshi, Eiichi Katagiri, Takashi |
| description | Exuberant smooth muscle cells (SMCs) hyperplasia is the major cause of postangioplasty restenosis. We suggested that circulating smooth muscle progenitor cells might contribute to lesion formation after vascular injury.
We extensively investigated the cellular constituents during neointimal formation after mechanical vascular injury.
A large wire was inserted into the mouse femoral artery, causing complete endothelial denudation and marked enlargement of the lumen with massive apoptosis of medial SMCs. At 2 h, the injured artery remained dilated with a thin media containing very few cells. A scanning electron microscopy showed fibrin and platelet deposition at the luminal side. One week after the injury, CD45-positive hematopoietic cells accumulated at the luminal side. Those CD45-positive cells gradually disappeared, whereas neointimal hyperplasia was formed with α-smooth muscle actin (SMA) positive cells. Bone marrow cells and peripheral mononuclear cells differentiated into α-SMA-positive cells in the presence of PDGF and basic FGF. Moreover, in bone marrow chimeric mice, bone-marrow-derived cells substantially contributed to neointimal hyperplasia after wire injury.
These results suggest that early accumulation of hematopoietic cells may play a role in the pathogenesis of SMC hyperplasia under certain circumstances. |
| doi_str_mv | 10.1016/j.carpath.2004.08.004 |
| format | Article |
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We extensively investigated the cellular constituents during neointimal formation after mechanical vascular injury.
A large wire was inserted into the mouse femoral artery, causing complete endothelial denudation and marked enlargement of the lumen with massive apoptosis of medial SMCs. At 2 h, the injured artery remained dilated with a thin media containing very few cells. A scanning electron microscopy showed fibrin and platelet deposition at the luminal side. One week after the injury, CD45-positive hematopoietic cells accumulated at the luminal side. Those CD45-positive cells gradually disappeared, whereas neointimal hyperplasia was formed with α-smooth muscle actin (SMA) positive cells. Bone marrow cells and peripheral mononuclear cells differentiated into α-SMA-positive cells in the presence of PDGF and basic FGF. Moreover, in bone marrow chimeric mice, bone-marrow-derived cells substantially contributed to neointimal hyperplasia after wire injury.
These results suggest that early accumulation of hematopoietic cells may play a role in the pathogenesis of SMC hyperplasia under certain circumstances.</description><identifier>ISSN: 1054-8807</identifier><identifier>EISSN: 1879-1336</identifier><identifier>DOI: 10.1016/j.carpath.2004.08.004</identifier><identifier>PMID: 15556776</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Actins - genetics ; Actins - metabolism ; Animals ; Bone Marrow Cells - cytology ; Bone Marrow Cells - metabolism ; Cell Differentiation ; Cell Proliferation ; Cells, Cultured ; Disease Models, Animal ; Femoral Artery - injuries ; Femoral Artery - metabolism ; Femoral Artery - ultrastructure ; Hematopoietic Stem Cells - metabolism ; Hematopoietic Stem Cells - pathology ; Hyperplasia ; Immunohistochemistry ; Injury ; Leukocyte Common Antigens - metabolism ; Leukocytes, Mononuclear - cytology ; Leukocytes, Mononuclear - metabolism ; Male ; Mice ; Mice, Inbred C3H ; Microscopy, Electron, Scanning ; Muscle, Smooth, Vascular - metabolism ; Muscle, Smooth, Vascular - pathology ; Muscle, Smooth, Vascular - physiopathology ; Myocytes, Smooth Muscle - metabolism ; Myocytes, Smooth Muscle - ultrastructure ; Neointima ; Progenitor ; Proliferation ; RNA, Messenger - metabolism ; Smooth muscle cell ; Tunica Intima - metabolism ; Tunica Intima - pathology ; Tunica Intima - physiopathology</subject><ispartof>Cardiovascular pathology, 2004-11, Vol.13 (6), p.306-312</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-2357de61859323ff25f17c52d1115e721dea64e8acd5fdb60a36d6d485b67b903</citedby><cites>FETCH-LOGICAL-c427t-2357de61859323ff25f17c52d1115e721dea64e8acd5fdb60a36d6d485b67b903</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.carpath.2004.08.004$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15556776$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shoji, Makoto</creatorcontrib><creatorcontrib>Sata, Masataka</creatorcontrib><creatorcontrib>Fukuda, Daiju</creatorcontrib><creatorcontrib>Tanaka, Kimie</creatorcontrib><creatorcontrib>Sato, Takatoshi</creatorcontrib><creatorcontrib>Iso, Yoshitaka</creatorcontrib><creatorcontrib>Shibata, Masayuki</creatorcontrib><creatorcontrib>Suzuki, Hiroshi</creatorcontrib><creatorcontrib>Koba, Shinji</creatorcontrib><creatorcontrib>Geshi, Eiichi</creatorcontrib><creatorcontrib>Katagiri, Takashi</creatorcontrib><title>Temporal and spatial characterization of cellular constituents during neointimal hyperplasia after vascular injury: Potential contribution of bone-marrow-derived progenitors to arterial remodeling</title><title>Cardiovascular pathology</title><addtitle>Cardiovasc Pathol</addtitle><description>Exuberant smooth muscle cells (SMCs) hyperplasia is the major cause of postangioplasty restenosis. We suggested that circulating smooth muscle progenitor cells might contribute to lesion formation after vascular injury.
We extensively investigated the cellular constituents during neointimal formation after mechanical vascular injury.
A large wire was inserted into the mouse femoral artery, causing complete endothelial denudation and marked enlargement of the lumen with massive apoptosis of medial SMCs. At 2 h, the injured artery remained dilated with a thin media containing very few cells. A scanning electron microscopy showed fibrin and platelet deposition at the luminal side. One week after the injury, CD45-positive hematopoietic cells accumulated at the luminal side. Those CD45-positive cells gradually disappeared, whereas neointimal hyperplasia was formed with α-smooth muscle actin (SMA) positive cells. Bone marrow cells and peripheral mononuclear cells differentiated into α-SMA-positive cells in the presence of PDGF and basic FGF. Moreover, in bone marrow chimeric mice, bone-marrow-derived cells substantially contributed to neointimal hyperplasia after wire injury.
These results suggest that early accumulation of hematopoietic cells may play a role in the pathogenesis of SMC hyperplasia under certain circumstances.</description><subject>Actins - genetics</subject><subject>Actins - metabolism</subject><subject>Animals</subject><subject>Bone Marrow Cells - cytology</subject><subject>Bone Marrow Cells - metabolism</subject><subject>Cell Differentiation</subject><subject>Cell Proliferation</subject><subject>Cells, Cultured</subject><subject>Disease Models, Animal</subject><subject>Femoral Artery - injuries</subject><subject>Femoral Artery - metabolism</subject><subject>Femoral Artery - ultrastructure</subject><subject>Hematopoietic Stem Cells - metabolism</subject><subject>Hematopoietic Stem Cells - pathology</subject><subject>Hyperplasia</subject><subject>Immunohistochemistry</subject><subject>Injury</subject><subject>Leukocyte Common Antigens - metabolism</subject><subject>Leukocytes, Mononuclear - cytology</subject><subject>Leukocytes, Mononuclear - metabolism</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Microscopy, Electron, Scanning</subject><subject>Muscle, Smooth, Vascular - metabolism</subject><subject>Muscle, Smooth, Vascular - pathology</subject><subject>Muscle, Smooth, Vascular - physiopathology</subject><subject>Myocytes, Smooth Muscle - metabolism</subject><subject>Myocytes, Smooth Muscle - ultrastructure</subject><subject>Neointima</subject><subject>Progenitor</subject><subject>Proliferation</subject><subject>RNA, Messenger - metabolism</subject><subject>Smooth muscle cell</subject><subject>Tunica Intima - metabolism</subject><subject>Tunica Intima - pathology</subject><subject>Tunica Intima - physiopathology</subject><issn>1054-8807</issn><issn>1879-1336</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUcmO1DAQjRCIWeATQD5xS7CTeGkuCI1gQBoJDsPZcuzKtFuJHcpOo-b7-DDc0404cqpS6S1V9arqFaMNo0y83TXW4GLytmkp7RuqmlKeVJdMyU3Nuk48LT3lfa0UlRfVVUo7Sqnq-_55dcE450JKcVn9vod5iWgmYoIjqQj60tutQWMzoP9VBjGQOBIL07ROBomNIWWfVwg5EbeiDw8kQPQh-7lwt4cFcJlM8oaYsWiQvUn2kenDbsXDO_It5kJ-NIohox_WvyZDDFDPBjH-rF2x34MjC8YHCD5HTCRHYvC4V-EizNHBVOxfVM9GMyV4ea7X1fdPH-9vPtd3X2-_3Hy4q23fyly3HZcOBFN807XdOLZ8ZNLy1jHGOMiWOTCiB2Ws46MbBDWdcML1ig9CDhvaXVdvTrplpR8rpKxnn45_MeX-NWkhqdq08gjkJ6DFmBLCqBcsz8GDZlQf49M7fY5PH-PTVOlSCu_12WAdZnD_WOe8CuD9CQDlzL0H1Ml6CBacR7BZu-j_Y_EHO2u2Kw</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Shoji, Makoto</creator><creator>Sata, Masataka</creator><creator>Fukuda, Daiju</creator><creator>Tanaka, Kimie</creator><creator>Sato, Takatoshi</creator><creator>Iso, Yoshitaka</creator><creator>Shibata, Masayuki</creator><creator>Suzuki, Hiroshi</creator><creator>Koba, Shinji</creator><creator>Geshi, Eiichi</creator><creator>Katagiri, Takashi</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Temporal and spatial characterization of cellular constituents during neointimal hyperplasia after vascular injury: Potential contribution of bone-marrow-derived progenitors to arterial remodeling</title><author>Shoji, Makoto ; Sata, Masataka ; Fukuda, Daiju ; Tanaka, Kimie ; Sato, Takatoshi ; Iso, Yoshitaka ; Shibata, Masayuki ; Suzuki, Hiroshi ; Koba, Shinji ; Geshi, Eiichi ; Katagiri, Takashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-2357de61859323ff25f17c52d1115e721dea64e8acd5fdb60a36d6d485b67b903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Actins - genetics</topic><topic>Actins - metabolism</topic><topic>Animals</topic><topic>Bone Marrow Cells - cytology</topic><topic>Bone Marrow Cells - metabolism</topic><topic>Cell Differentiation</topic><topic>Cell Proliferation</topic><topic>Cells, Cultured</topic><topic>Disease Models, Animal</topic><topic>Femoral Artery - injuries</topic><topic>Femoral Artery - metabolism</topic><topic>Femoral Artery - ultrastructure</topic><topic>Hematopoietic Stem Cells - metabolism</topic><topic>Hematopoietic Stem Cells - pathology</topic><topic>Hyperplasia</topic><topic>Immunohistochemistry</topic><topic>Injury</topic><topic>Leukocyte Common Antigens - metabolism</topic><topic>Leukocytes, Mononuclear - cytology</topic><topic>Leukocytes, Mononuclear - metabolism</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Microscopy, Electron, Scanning</topic><topic>Muscle, Smooth, Vascular - metabolism</topic><topic>Muscle, Smooth, Vascular - pathology</topic><topic>Muscle, Smooth, Vascular - physiopathology</topic><topic>Myocytes, Smooth Muscle - metabolism</topic><topic>Myocytes, Smooth Muscle - ultrastructure</topic><topic>Neointima</topic><topic>Progenitor</topic><topic>Proliferation</topic><topic>RNA, Messenger - metabolism</topic><topic>Smooth muscle cell</topic><topic>Tunica Intima - metabolism</topic><topic>Tunica Intima - pathology</topic><topic>Tunica Intima - physiopathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shoji, Makoto</creatorcontrib><creatorcontrib>Sata, Masataka</creatorcontrib><creatorcontrib>Fukuda, Daiju</creatorcontrib><creatorcontrib>Tanaka, Kimie</creatorcontrib><creatorcontrib>Sato, Takatoshi</creatorcontrib><creatorcontrib>Iso, Yoshitaka</creatorcontrib><creatorcontrib>Shibata, Masayuki</creatorcontrib><creatorcontrib>Suzuki, Hiroshi</creatorcontrib><creatorcontrib>Koba, Shinji</creatorcontrib><creatorcontrib>Geshi, Eiichi</creatorcontrib><creatorcontrib>Katagiri, Takashi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cardiovascular pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shoji, Makoto</au><au>Sata, Masataka</au><au>Fukuda, Daiju</au><au>Tanaka, Kimie</au><au>Sato, Takatoshi</au><au>Iso, Yoshitaka</au><au>Shibata, Masayuki</au><au>Suzuki, Hiroshi</au><au>Koba, Shinji</au><au>Geshi, Eiichi</au><au>Katagiri, Takashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Temporal and spatial characterization of cellular constituents during neointimal hyperplasia after vascular injury: Potential contribution of bone-marrow-derived progenitors to arterial remodeling</atitle><jtitle>Cardiovascular pathology</jtitle><addtitle>Cardiovasc Pathol</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>13</volume><issue>6</issue><spage>306</spage><epage>312</epage><pages>306-312</pages><issn>1054-8807</issn><eissn>1879-1336</eissn><abstract>Exuberant smooth muscle cells (SMCs) hyperplasia is the major cause of postangioplasty restenosis. We suggested that circulating smooth muscle progenitor cells might contribute to lesion formation after vascular injury.
We extensively investigated the cellular constituents during neointimal formation after mechanical vascular injury.
A large wire was inserted into the mouse femoral artery, causing complete endothelial denudation and marked enlargement of the lumen with massive apoptosis of medial SMCs. At 2 h, the injured artery remained dilated with a thin media containing very few cells. A scanning electron microscopy showed fibrin and platelet deposition at the luminal side. One week after the injury, CD45-positive hematopoietic cells accumulated at the luminal side. Those CD45-positive cells gradually disappeared, whereas neointimal hyperplasia was formed with α-smooth muscle actin (SMA) positive cells. Bone marrow cells and peripheral mononuclear cells differentiated into α-SMA-positive cells in the presence of PDGF and basic FGF. Moreover, in bone marrow chimeric mice, bone-marrow-derived cells substantially contributed to neointimal hyperplasia after wire injury.
These results suggest that early accumulation of hematopoietic cells may play a role in the pathogenesis of SMC hyperplasia under certain circumstances.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15556776</pmid><doi>10.1016/j.carpath.2004.08.004</doi><tpages>7</tpages></addata></record> |
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| subjects | Actins - genetics Actins - metabolism Animals Bone Marrow Cells - cytology Bone Marrow Cells - metabolism Cell Differentiation Cell Proliferation Cells, Cultured Disease Models, Animal Femoral Artery - injuries Femoral Artery - metabolism Femoral Artery - ultrastructure Hematopoietic Stem Cells - metabolism Hematopoietic Stem Cells - pathology Hyperplasia Immunohistochemistry Injury Leukocyte Common Antigens - metabolism Leukocytes, Mononuclear - cytology Leukocytes, Mononuclear - metabolism Male Mice Mice, Inbred C3H Microscopy, Electron, Scanning Muscle, Smooth, Vascular - metabolism Muscle, Smooth, Vascular - pathology Muscle, Smooth, Vascular - physiopathology Myocytes, Smooth Muscle - metabolism Myocytes, Smooth Muscle - ultrastructure Neointima Progenitor Proliferation RNA, Messenger - metabolism Smooth muscle cell Tunica Intima - metabolism Tunica Intima - pathology Tunica Intima - physiopathology |
| title | Temporal and spatial characterization of cellular constituents during neointimal hyperplasia after vascular injury: Potential contribution of bone-marrow-derived progenitors to arterial remodeling |
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