Production of monoclonal antibodies and development of an antigen capture ELISA directed against the envelope glycoprotein GP of Ebola virus

Ebola virus (EBOV) causes severe outbreaks of Ebola hemorrhagic fever in endemic regions of Africa and is considered to be of impact for other parts of the world as an imported viral disease. To develop a new diagnostic test, monoclonal antibodies to EBOV were produced from mice immunized with inact...

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Veröffentlicht in:	Medical microbiology and immunology 2004-11, Vol.193 (4), p.181-187
Hauptverfasser:	LUCHT, Andreas, GRUNOW, Roland, OTTERBEIN, Christian, MÖLLER, Peggy, FELDMANN, Heinz, BECKER, Stephan
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibodies, Monoclonal - immunology Antibodies, Viral - immunology Applied microbiology Biological and medical sciences Diagnostic tests Ebola virus Ebolavirus Ebolavirus - immunology Ebolavirus - isolation & purification Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Fever Fundamental and applied biological sciences. Psychology Glycoproteins HeLa Cells Hemorrhage Hemorrhagic fever Hemorrhagic Fever, Ebola - diagnosis Human viral diseases Humans Immunofluorescence Infectious diseases Marburg disease, ebola disease Medical diagnosis Medical sciences Mice Mice, Inbred BALB C Microbiology Miscellaneous Monoclonal antibodies Sensitivity and Specificity Sodium Dodecyl Sulfate - pharmacology Surface-Active Agents - pharmacology Tropical viral diseases Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Viral diseases Viral Envelope Proteins - analysis Viral Envelope Proteins - immunology Virology Virus Inactivation
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