Identification of CCDC62‐2 as a novel cancer/testis antigen and its immunogenicity
Cancer/testis (CT) antigens are expressed in normal germ line tissues and various cancers. They are considered promising target molecules for immunotherapy for patients with various cancers. To identify CT antigens, we performed serological identification of antigens by recombinant expression clonin...
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| Veröffentlicht in: | International journal of cancer 2009-05, Vol.124 (10), p.2347-2352 |
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| creator | Domae, Shohei Nakamura, Yoichi Nakamura, Yurika Uenaka, Akiko Wada, Hisashi Nakata, Masao Oka, Mikio Kishimoto, Koji Tsukamoto, Goichi Yoshihama, Yasuto Matsuoka, Junji Gochi, Akira Kohno, Shigeru Saika, Takashi Sasaki, Akira Nakayama, Eiichi Ono, Toshiro |
| description | Cancer/testis (CT) antigens are expressed in normal germ line tissues and various cancers. They are considered promising target molecules for immunotherapy for patients with various cancers. To identify CT antigens, we performed serological identification of antigens by recombinant expression cloning. The humoral immune response of cancer patients against a newly defined antigen was analyzed. A testicular cDNA library was immunoscreened with serum obtained from a gastric adenocarcinoma patient whose primary cancer had regressed once and most liver metastasies had disappeared transiently. We isolated 55 positive cDNA clones comprising 23 different genes. They included 4 genes with testis‐specific expression profiles in the Unigene database, including coiled‐coil domain containing 62 (CCDC62). RT‐PCR analysis showed that the expression of 2 splice variants of CCDC62 was restricted to the testis in normal adult tissues. In malignant tissues, CCDC62 variant 2 (CCDC62‐2) was aberrantly expressed in a variety of cancers, including stomach cancer. A serological survey of 191 cancer patients with a range of different cancers by ELISA revealed antibodies to CCDC62‐2 in 13 patients, including stomach cancer. None of the 41 healthy donor serum samples were reactive in the same test. The serum reaction against CCDC62‐2 was confirmed by western blot. CCDC62‐2 is a CT antigen that is immunogenic in cancer patients. © 2008 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/ijc.24220 |
| format | Article |
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They are considered promising target molecules for immunotherapy for patients with various cancers. To identify CT antigens, we performed serological identification of antigens by recombinant expression cloning. The humoral immune response of cancer patients against a newly defined antigen was analyzed. A testicular cDNA library was immunoscreened with serum obtained from a gastric adenocarcinoma patient whose primary cancer had regressed once and most liver metastasies had disappeared transiently. We isolated 55 positive cDNA clones comprising 23 different genes. They included 4 genes with testis‐specific expression profiles in the Unigene database, including coiled‐coil domain containing 62 (CCDC62). RT‐PCR analysis showed that the expression of 2 splice variants of CCDC62 was restricted to the testis in normal adult tissues. In malignant tissues, CCDC62 variant 2 (CCDC62‐2) was aberrantly expressed in a variety of cancers, including stomach cancer. A serological survey of 191 cancer patients with a range of different cancers by ELISA revealed antibodies to CCDC62‐2 in 13 patients, including stomach cancer. None of the 41 healthy donor serum samples were reactive in the same test. The serum reaction against CCDC62‐2 was confirmed by western blot. CCDC62‐2 is a CT antigen that is immunogenic in cancer patients. © 2008 Wiley‐Liss, Inc.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.24220</identifier><identifier>PMID: 19165854</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adenocarcinoma - immunology ; Aged ; antibody response ; Antigens, Neoplasm - biosynthesis ; Antigens, Neoplasm - genetics ; Antigens, Neoplasm - immunology ; Base Sequence ; Biological and medical sciences ; Blotting, Western ; cancer/testis antigen ; CCDC62‐2 ; DNA Primers ; DNA, Complementary ; Enzyme-Linked Immunosorbent Assay ; Humans ; Male ; Medical sciences ; Recombinant Proteins - biosynthesis ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Messenger - genetics ; SEREX ; Stomach Neoplasms - immunology ; Testis - immunology ; Transcription Factors - biosynthesis ; Transcription Factors - genetics ; Transcription Factors - immunology ; Tumors</subject><ispartof>International journal of cancer, 2009-05, Vol.124 (10), p.2347-2352</ispartof><rights>Copyright © 2009 Wiley‐Liss, Inc.</rights><rights>2009 INIST-CNRS</rights><rights>(c) 2008 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4850-cc383f44c91e23c7c6388ed0656d8ffeed1c1aca280fa2425c6cb1e4cdd544133</citedby><cites>FETCH-LOGICAL-c4850-cc383f44c91e23c7c6388ed0656d8ffeed1c1aca280fa2425c6cb1e4cdd544133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.24220$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.24220$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21303804$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19165854$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Domae, Shohei</creatorcontrib><creatorcontrib>Nakamura, Yoichi</creatorcontrib><creatorcontrib>Nakamura, Yurika</creatorcontrib><creatorcontrib>Uenaka, Akiko</creatorcontrib><creatorcontrib>Wada, Hisashi</creatorcontrib><creatorcontrib>Nakata, Masao</creatorcontrib><creatorcontrib>Oka, Mikio</creatorcontrib><creatorcontrib>Kishimoto, Koji</creatorcontrib><creatorcontrib>Tsukamoto, Goichi</creatorcontrib><creatorcontrib>Yoshihama, Yasuto</creatorcontrib><creatorcontrib>Matsuoka, Junji</creatorcontrib><creatorcontrib>Gochi, Akira</creatorcontrib><creatorcontrib>Kohno, Shigeru</creatorcontrib><creatorcontrib>Saika, Takashi</creatorcontrib><creatorcontrib>Sasaki, Akira</creatorcontrib><creatorcontrib>Nakayama, Eiichi</creatorcontrib><creatorcontrib>Ono, Toshiro</creatorcontrib><title>Identification of CCDC62‐2 as a novel cancer/testis antigen and its immunogenicity</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>Cancer/testis (CT) antigens are expressed in normal germ line tissues and various cancers. They are considered promising target molecules for immunotherapy for patients with various cancers. To identify CT antigens, we performed serological identification of antigens by recombinant expression cloning. The humoral immune response of cancer patients against a newly defined antigen was analyzed. A testicular cDNA library was immunoscreened with serum obtained from a gastric adenocarcinoma patient whose primary cancer had regressed once and most liver metastasies had disappeared transiently. We isolated 55 positive cDNA clones comprising 23 different genes. They included 4 genes with testis‐specific expression profiles in the Unigene database, including coiled‐coil domain containing 62 (CCDC62). RT‐PCR analysis showed that the expression of 2 splice variants of CCDC62 was restricted to the testis in normal adult tissues. In malignant tissues, CCDC62 variant 2 (CCDC62‐2) was aberrantly expressed in a variety of cancers, including stomach cancer. A serological survey of 191 cancer patients with a range of different cancers by ELISA revealed antibodies to CCDC62‐2 in 13 patients, including stomach cancer. None of the 41 healthy donor serum samples were reactive in the same test. The serum reaction against CCDC62‐2 was confirmed by western blot. 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They are considered promising target molecules for immunotherapy for patients with various cancers. To identify CT antigens, we performed serological identification of antigens by recombinant expression cloning. The humoral immune response of cancer patients against a newly defined antigen was analyzed. A testicular cDNA library was immunoscreened with serum obtained from a gastric adenocarcinoma patient whose primary cancer had regressed once and most liver metastasies had disappeared transiently. We isolated 55 positive cDNA clones comprising 23 different genes. They included 4 genes with testis‐specific expression profiles in the Unigene database, including coiled‐coil domain containing 62 (CCDC62). RT‐PCR analysis showed that the expression of 2 splice variants of CCDC62 was restricted to the testis in normal adult tissues. In malignant tissues, CCDC62 variant 2 (CCDC62‐2) was aberrantly expressed in a variety of cancers, including stomach cancer. A serological survey of 191 cancer patients with a range of different cancers by ELISA revealed antibodies to CCDC62‐2 in 13 patients, including stomach cancer. None of the 41 healthy donor serum samples were reactive in the same test. The serum reaction against CCDC62‐2 was confirmed by western blot. CCDC62‐2 is a CT antigen that is immunogenic in cancer patients. © 2008 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>19165854</pmid><doi>10.1002/ijc.24220</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adenocarcinoma - immunology Aged antibody response Antigens, Neoplasm - biosynthesis Antigens, Neoplasm - genetics Antigens, Neoplasm - immunology Base Sequence Biological and medical sciences Blotting, Western cancer/testis antigen CCDC62‐2 DNA Primers DNA, Complementary Enzyme-Linked Immunosorbent Assay Humans Male Medical sciences Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Recombinant Proteins - immunology Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - genetics SEREX Stomach Neoplasms - immunology Testis - immunology Transcription Factors - biosynthesis Transcription Factors - genetics Transcription Factors - immunology Tumors |
| title | Identification of CCDC62‐2 as a novel cancer/testis antigen and its immunogenicity |
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