Fluorescence in situ hybridization for intracellular localization of nifH mRNA

Few reports on in situ mRNA detection in bacteria have been published, even though a major aim in environmental microbiology is to link function/activity to the identity of the organisms. This study reports a reliable approach for the in situ detection of nifH mRNA using fluorescence hybridization b...

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Veröffentlicht in:	Systematic and applied microbiology 2009-05, Vol.32 (3), p.186-192
Hauptverfasser:	Pilhofer, Martin, Pavlekovic, Marko, Lee, Natuschka M., Ludwig, Wolfgang, Schleifer, Karl-Heinz
Format:	Artikel
Sprache:	eng
Schlagworte:	Azotobacter vinelandii - classification Azotobacter vinelandii - enzymology Azotobacter vinelandii - genetics Azotobacter vinelandii - isolation & purification Bacterial Typing Techniques Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Catalyzed reporter deposition Diazotrophs Fluorescence in situ hybridization (FISH) Fundamental and applied biological sciences. Psychology In Situ Hybridization, Fluorescence - methods Klebsiella oxytoca - classification Klebsiella oxytoca - enzymology Klebsiella oxytoca - genetics Klebsiella oxytoca - isolation & purification Microbiology mRNA FISH Nitrogen Fixation Oligonucleotide Probes Oxidoreductases - genetics Oxidoreductases - metabolism RNA, Messenger - genetics RNA, Messenger - metabolism
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