Pair-wise detection of eight common DNA polymorphisms of the human lipoprotein lipase gene by PCR-RFLP. Findings of the Olivetti Heart Study
We developed a rapid procedure to analyse simultaneously two different DNA polymorphisms of the human LPL gene by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). The method involves PCR amplification of the gene fragments encompassing two polymorphic sites, direct dige...
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| Veröffentlicht in: | Nutrition, metabolism, and cardiovascular diseases metabolism, and cardiovascular diseases, 2004-08, Vol.14 (4), p.205-210 |
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| creator | Feliciello, I. Capossela, S. Cantile, M. Barbato, A. Cappuccio, F.P. Strazzullo, P. Chinali, G. |
| description | We developed a rapid procedure to analyse simultaneously two different DNA polymorphisms of the human LPL gene by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). The method involves PCR amplification of the gene fragments encompassing two polymorphic sites, direct digestion in the same PCR-tube of the amplification mixture with two restriction enzymes, and the analysis of the resulting DNA fragments by gel electrophoresis. In 422 participants of the 1994 follow-up examination of the Olivetti Heart Study, a total of eight common LPL gene polymorphisms have been analysed in pairs by this procedure: −93 T/G and D9N; V108V and T361T; N291S and
PvuII; HindIII and S447X. Two of these polymorphisms (V108V and T361T) were analysed for the first time.
This method is suitable for the routine analysis of clinical samples of varying DNA content and practically halves the times and costs of screening for these LPL polymorphisms. |
| doi_str_mv | 10.1016/S0939-4753(04)80006-1 |
| format | Article |
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PvuII; HindIII and S447X. Two of these polymorphisms (V108V and T361T) were analysed for the first time.
This method is suitable for the routine analysis of clinical samples of varying DNA content and practically halves the times and costs of screening for these LPL polymorphisms.</description><identifier>ISSN: 0939-4753</identifier><identifier>EISSN: 1590-3729</identifier><identifier>DOI: 10.1016/S0939-4753(04)80006-1</identifier><identifier>PMID: 15553598</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Adult ; Aged ; Base Sequence ; DNA - analysis ; DNA Primers ; Exons ; Gene Amplification ; Gene Frequency ; genotyping ; Humans ; Introns ; Lipoprotein Lipase - genetics ; Lipoprotein lipase gene polymorphisms ; Male ; Middle Aged ; Molecular Sequence Data ; Olivetti Heart Study ; Polymerase Chain Reaction ; Polymorphism, Restriction Fragment Length ; restriction fragment length polymorphism</subject><ispartof>Nutrition, metabolism, and cardiovascular diseases, 2004-08, Vol.14 (4), p.205-210</ispartof><rights>2004 Elsevier Science</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-34904cf984ae33539200fd5bc422ed98e3d0187c29d70636fa010683b0d38cca3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0939-4753(04)80006-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15553598$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Feliciello, I.</creatorcontrib><creatorcontrib>Capossela, S.</creatorcontrib><creatorcontrib>Cantile, M.</creatorcontrib><creatorcontrib>Barbato, A.</creatorcontrib><creatorcontrib>Cappuccio, F.P.</creatorcontrib><creatorcontrib>Strazzullo, P.</creatorcontrib><creatorcontrib>Chinali, G.</creatorcontrib><title>Pair-wise detection of eight common DNA polymorphisms of the human lipoprotein lipase gene by PCR-RFLP. Findings of the Olivetti Heart Study</title><title>Nutrition, metabolism, and cardiovascular diseases</title><addtitle>Nutr Metab Cardiovasc Dis</addtitle><description>We developed a rapid procedure to analyse simultaneously two different DNA polymorphisms of the human LPL gene by PCR-RFLP (Polymerase Chain Reaction-Restriction Fragment Length Polymorphism). The method involves PCR amplification of the gene fragments encompassing two polymorphic sites, direct digestion in the same PCR-tube of the amplification mixture with two restriction enzymes, and the analysis of the resulting DNA fragments by gel electrophoresis. In 422 participants of the 1994 follow-up examination of the Olivetti Heart Study, a total of eight common LPL gene polymorphisms have been analysed in pairs by this procedure: −93 T/G and D9N; V108V and T361T; N291S and
PvuII; HindIII and S447X. Two of these polymorphisms (V108V and T361T) were analysed for the first time.
This method is suitable for the routine analysis of clinical samples of varying DNA content and practically halves the times and costs of screening for these LPL polymorphisms.</description><subject>Adult</subject><subject>Aged</subject><subject>Base Sequence</subject><subject>DNA - analysis</subject><subject>DNA Primers</subject><subject>Exons</subject><subject>Gene Amplification</subject><subject>Gene Frequency</subject><subject>genotyping</subject><subject>Humans</subject><subject>Introns</subject><subject>Lipoprotein Lipase - genetics</subject><subject>Lipoprotein lipase gene polymorphisms</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Olivetti Heart Study</subject><subject>Polymerase Chain Reaction</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>restriction fragment length polymorphism</subject><issn>0939-4753</issn><issn>1590-3729</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkdtu1DAQhi1ERZfCI4B8heAiZRw7B1-hasvSSiu6auHa8tqTXaMkDrbTat-BhyZ7ULnkamak75_TT8g7BpcMWPn5ASSXmagK_hHEpxoAyoy9IDNWSMh4lcuXZPaMnJPXMf4C4BVw8Yqcs6IoeCHrGfmz0i5kTy4itZjQJOd76huKbrNN1Pium-rr71d08O2u82HYutjFPZG2SLdjp3vausEPwSd0h1xPvTbYI13v6Gp-n90vlqtLunC9df3mWXrXukdMydEb1CHRhzTa3Rty1ug24ttTvCA_F19_zG-y5d232_nVMjO8ZCnjQoIwjayFRs4LLnOAxhZrI_IcrayRW2B1ZXJpKyh52WhgUNZ8DZbXxmh-QT4c-05b_x4xJtW5aLBtdY9-jKqsoBasLCewOIIm-BgDNmoIrtNhpxiovQ3qYIPa_1iBUAcbFJt0708DxnWH9p_q9PcJ-HIEcDrz0WFQ0TjsDVoXJhOU9e4_I_4CXGOXtA</recordid><startdate>20040801</startdate><enddate>20040801</enddate><creator>Feliciello, I.</creator><creator>Capossela, S.</creator><creator>Cantile, M.</creator><creator>Barbato, A.</creator><creator>Cappuccio, F.P.</creator><creator>Strazzullo, P.</creator><creator>Chinali, G.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20040801</creationdate><title>Pair-wise detection of eight common DNA polymorphisms of the human lipoprotein lipase gene by PCR-RFLP. 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In 422 participants of the 1994 follow-up examination of the Olivetti Heart Study, a total of eight common LPL gene polymorphisms have been analysed in pairs by this procedure: −93 T/G and D9N; V108V and T361T; N291S and
PvuII; HindIII and S447X. Two of these polymorphisms (V108V and T361T) were analysed for the first time.
This method is suitable for the routine analysis of clinical samples of varying DNA content and practically halves the times and costs of screening for these LPL polymorphisms.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15553598</pmid><doi>10.1016/S0939-4753(04)80006-1</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Adult Aged Base Sequence DNA - analysis DNA Primers Exons Gene Amplification Gene Frequency genotyping Humans Introns Lipoprotein Lipase - genetics Lipoprotein lipase gene polymorphisms Male Middle Aged Molecular Sequence Data Olivetti Heart Study Polymerase Chain Reaction Polymorphism, Restriction Fragment Length restriction fragment length polymorphism |
| title | Pair-wise detection of eight common DNA polymorphisms of the human lipoprotein lipase gene by PCR-RFLP. Findings of the Olivetti Heart Study |
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