Label-free direct detection of MiRNAs with silicon nanowire biosensors
MicroRNA (miRNA), an 18–24-nucleotide (nt) noncoding RNA molecule in the genes of humans, plants and animals, is emerging as a key player in gene regulation. As a result, label-free, rapid, and sensitive detection for miRNA is of great significance. In this work, a label-free and direct hybridizatio...
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| Veröffentlicht in: | Biosensors & bioelectronics 2009-04, Vol.24 (8), p.2504-2508 |
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| creator | Zhang, Guo-Jun Chua, Jay Huiyi Chee, Ru-Ern Agarwal, Ajay Wong, She Mein |
| description | MicroRNA (miRNA), an 18–24-nucleotide (nt) noncoding RNA molecule in the genes of humans, plants and animals, is emerging as a key player in gene regulation. As a result, label-free, rapid, and sensitive detection for miRNA is of great significance. In this work, a label-free and direct hybridization assay for ultrasensitive detection of miRNA using silicon nanowires (SiNWs) device has been developed. Peptide nucleic acids (PNAs), which serve as a receptor to recognize miRNA directly without labeling the target miRNA, are immobilized on the surface of the SiNW device. Resistance change measured before and after hybridization correlates directly to concentrations of the hybridized target miRNA. Concentration-dependent measurements indicate that a detection limit of 1fM was obtained using the optimized assay. The technique enables identification of fully matched versus mismatched miRNA sequences. Furthermore, the SiNW device is capable of detecting miRNA in total RNA extracted from Hela cells. This approach paves a way for label-free, early detection of miRNA as a biomarker in cancer diagnostics with very high sensitivity and good specificity. |
| doi_str_mv | 10.1016/j.bios.2008.12.035 |
| format | Article |
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As a result, label-free, rapid, and sensitive detection for miRNA is of great significance. In this work, a label-free and direct hybridization assay for ultrasensitive detection of miRNA using silicon nanowires (SiNWs) device has been developed. Peptide nucleic acids (PNAs), which serve as a receptor to recognize miRNA directly without labeling the target miRNA, are immobilized on the surface of the SiNW device. Resistance change measured before and after hybridization correlates directly to concentrations of the hybridized target miRNA. Concentration-dependent measurements indicate that a detection limit of 1fM was obtained using the optimized assay. The technique enables identification of fully matched versus mismatched miRNA sequences. Furthermore, the SiNW device is capable of detecting miRNA in total RNA extracted from Hela cells. 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Technologies</subject><subject>MicroRNA</subject><subject>MicroRNAs - analysis</subject><subject>MicroRNAs - chemistry</subject><subject>MicroRNAs - genetics</subject><subject>Nanotubes - chemistry</subject><subject>Nanotubes - ultrastructure</subject><subject>Peptide nucleic acid</subject><subject>Peptide Nucleic Acids - chemistry</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Silicon - chemistry</subject><subject>Silicon nanowires</subject><subject>Staining and Labeling</subject><subject>Various methods and equipments</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9rFDEUgENR7Fr9B3ooc9HbjEkmmSTQSynWCqtCqeeQeXnBLLOTNpm1-N-bZRd7q6cHj-_9-h4h54x2jLLh06YbYyodp1R3jHe0lydkxbTqW8F7-YqsqJFDK4ehPyVvS9lQShUz9A05ZYZpTaVekZu1G3FqQ0ZsfMwIS-NxqSGmuUmh-Rbvvl-V5ikuv5oSpwg1Pbs5PVW22Y_HuaRc3pHXwU0F3x_jGfl58_n--rZd__jy9fpq3YJgcmk996EftZTgPQrnqBq0HCGAEVwYLUEDgBi5MUxp1zMdzAhCaeWdAwisPyMfD30fcnrcYVnsNhbAaXIzpl2xg6L1Lv5_kFOhBqP6CvIDCDmVkjHYhxy3Lv-xjNq9Zrux-zvtXrNl3FbNteji2H03btE_lxy9VuDDEXAF3BSymyGWfxxnghplTOUuDxxWab8jZlsg4gx4-IX1Kb60x1_DCJuT</recordid><startdate>20090415</startdate><enddate>20090415</enddate><creator>Zhang, Guo-Jun</creator><creator>Chua, Jay Huiyi</creator><creator>Chee, Ru-Ern</creator><creator>Agarwal, Ajay</creator><creator>Wong, She Mein</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20090415</creationdate><title>Label-free direct detection of MiRNAs with silicon nanowire biosensors</title><author>Zhang, Guo-Jun ; Chua, Jay Huiyi ; Chee, Ru-Ern ; Agarwal, Ajay ; Wong, She Mein</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c415t-d2df3b855cdde4aa07685bcfc9424985c8ccc4b299178a318f9bc4787daaccf13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biosensors</topic><topic>Biotechnology</topic><topic>Electric Conductivity</topic><topic>Electrochemistry - instrumentation</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fundamental and applied biological sciences. 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As a result, label-free, rapid, and sensitive detection for miRNA is of great significance. In this work, a label-free and direct hybridization assay for ultrasensitive detection of miRNA using silicon nanowires (SiNWs) device has been developed. Peptide nucleic acids (PNAs), which serve as a receptor to recognize miRNA directly without labeling the target miRNA, are immobilized on the surface of the SiNW device. Resistance change measured before and after hybridization correlates directly to concentrations of the hybridized target miRNA. Concentration-dependent measurements indicate that a detection limit of 1fM was obtained using the optimized assay. The technique enables identification of fully matched versus mismatched miRNA sequences. Furthermore, the SiNW device is capable of detecting miRNA in total RNA extracted from Hela cells. 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| subjects | Biological and medical sciences Biosensing Techniques - instrumentation Biosensors Biotechnology Electric Conductivity Electrochemistry - instrumentation Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology Hybridization In Situ Hybridization - instrumentation In Situ Hybridization - methods Label-free detection Methods. Procedures. Technologies MicroRNA MicroRNAs - analysis MicroRNAs - chemistry MicroRNAs - genetics Nanotubes - chemistry Nanotubes - ultrastructure Peptide nucleic acid Peptide Nucleic Acids - chemistry Reproducibility of Results Sensitivity and Specificity Silicon - chemistry Silicon nanowires Staining and Labeling Various methods and equipments |
| title | Label-free direct detection of MiRNAs with silicon nanowire biosensors |
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