Food allergy/hypersensitivity: Antigenicity or timing?
Abstract Mechanisms involved in the induction of oral tolerance (OT) or systemic immunization through the oral rout are still poorly understood. In our previous studies, we have shown that when normal mice eat peanuts they become tolerant, with no gut alterations. Conversely, if immunized with peanu...
Gespeichert in:
Veröffentlicht in: | Immunobiology (1979) 2009-04, Vol.214 (4), p.269-278 |
---|---|
Hauptverfasser: | , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 278 |
---|---|
container_issue | 4 |
container_start_page | 269 |
container_title | Immunobiology (1979) |
container_volume | 214 |
creator | Paschoal, Patrícia Olaya Campos, Sylvia M.N Pedruzzi, Monique M.B Garrido, Valéria Bisso, Mônica Antunes, Danielle M.F Nobrega, Alberto F Teixeira, Gerlinde |
description | Abstract Mechanisms involved in the induction of oral tolerance (OT) or systemic immunization through the oral rout are still poorly understood. In our previous studies, we have shown that when normal mice eat peanuts they become tolerant, with no gut alterations. Conversely, if immunized with peanut proteins prior to a challenge diet (CD) containing peanuts they develop chronic inflammation of the gut. Our aim is to evaluate the consequences of the introduction of a novel protein in the diet of animals presenting antigen-specific gut inflammation. Adult, female C57BL/6J mice were divided in control (C) and experimental (E) groups. C1–C3 received peanut protein immunization, animals of the control groups C4 were sham immunized, and control group C5 received ovalbumin (OVA) immunization. The experimental group was immunized with peanut protein extract. Before initial exposure to a 30-day peanut containing CD, the experimental group was divided into 5 groups (E1–E5). OVA feeding began 7 days prior CD (E1) on day 0 (E2), 7 (E3), 14 (E4) and 21 (E5) during CD. Our results show that oral exposure to a novel protein (OVA) in the absence of gut inflammation (E1) leads to low levels of systemic antibody (Ab) titers, comparable to tolerant animals. Conversely, as off initial induction of inflammation, groups submitted to OVA (OT) protocol develop increasingly higher systemic Ab titers similar to animals of the immune control group. In conclusion, our protocol indicates that timing is more important than the antigenicity when a novel protein is offered, in the diet. |
doi_str_mv | 10.1016/j.imbio.2008.09.007 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67077946</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>1_s2_0_S0171298508001290</els_id><sourcerecordid>20530977</sourcerecordid><originalsourceid>FETCH-LOGICAL-c443t-8e01e2d20afd9300e66887285e0f1ca0b09a9702208e6985234cf4669d515c193</originalsourceid><addsrcrecordid>eNqFkV1LwzAUhoMobn78AkF25V3rSdrmQ1ARcSoIXqjXoUtPZ2bbzKQT-u_N3EDwxqtw4HlPDs9LyAmFlALl54vUtjPrUgYgU1ApgNghYyqFTDIm1C4ZAxU0YUoWI3IQwgKAKibkPhlRFYkiz8eET52rJmXToJ8P5-_DEn3ALtjeftl-uJjcdL2dY2dNnCbOT3rb2m5-fUT26rIJeLx9D8nb9O719iF5er5_vL15SkyeZ30iESiyikFZVyoDQM6lFEwWCDU1JcxAlUoAYyCRxztZlps651xVBS1MvPKQnG32Lr37XGHodWuDwaYpO3SroLkAIVTO_wUZFBkoISKYbUDjXQgea730ti39oCnotVe90D9e9dqrBqWj15g63a5fzVqsfjNbkRG43AAYbXxZ9DoYi53Byno0va6c_eeDqz9509iovWw-cMCwcCvfRdGa6sA06Jd1tetmQcZWmYLsG7BknV4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>20530977</pqid></control><display><type>article</type><title>Food allergy/hypersensitivity: Antigenicity or timing?</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Paschoal, Patrícia Olaya ; Campos, Sylvia M.N ; Pedruzzi, Monique M.B ; Garrido, Valéria ; Bisso, Mônica ; Antunes, Danielle M.F ; Nobrega, Alberto F ; Teixeira, Gerlinde</creator><creatorcontrib>Paschoal, Patrícia Olaya ; Campos, Sylvia M.N ; Pedruzzi, Monique M.B ; Garrido, Valéria ; Bisso, Mônica ; Antunes, Danielle M.F ; Nobrega, Alberto F ; Teixeira, Gerlinde</creatorcontrib><description>Abstract Mechanisms involved in the induction of oral tolerance (OT) or systemic immunization through the oral rout are still poorly understood. In our previous studies, we have shown that when normal mice eat peanuts they become tolerant, with no gut alterations. Conversely, if immunized with peanut proteins prior to a challenge diet (CD) containing peanuts they develop chronic inflammation of the gut. Our aim is to evaluate the consequences of the introduction of a novel protein in the diet of animals presenting antigen-specific gut inflammation. Adult, female C57BL/6J mice were divided in control (C) and experimental (E) groups. C1–C3 received peanut protein immunization, animals of the control groups C4 were sham immunized, and control group C5 received ovalbumin (OVA) immunization. The experimental group was immunized with peanut protein extract. Before initial exposure to a 30-day peanut containing CD, the experimental group was divided into 5 groups (E1–E5). OVA feeding began 7 days prior CD (E1) on day 0 (E2), 7 (E3), 14 (E4) and 21 (E5) during CD. Our results show that oral exposure to a novel protein (OVA) in the absence of gut inflammation (E1) leads to low levels of systemic antibody (Ab) titers, comparable to tolerant animals. Conversely, as off initial induction of inflammation, groups submitted to OVA (OT) protocol develop increasingly higher systemic Ab titers similar to animals of the immune control group. In conclusion, our protocol indicates that timing is more important than the antigenicity when a novel protein is offered, in the diet.</description><identifier>ISSN: 0171-2985</identifier><identifier>EISSN: 1878-3279</identifier><identifier>DOI: 10.1016/j.imbio.2008.09.007</identifier><identifier>PMID: 19327544</identifier><language>eng</language><publisher>Netherlands: Elsevier GmbH</publisher><subject>Administration, Oral ; Advanced Basic Science ; Allergy and Immunology ; Animals ; Antibody Formation ; Antigens, Plant - administration & dosage ; Antigens, Plant - immunology ; Arachis - immunology ; Arachis hypogaea ; Diet ; Epitopes ; Female ; Food allergy ; Food Hypersensitivity - immunology ; Food Hypersensitivity - physiopathology ; Humans ; Immune Tolerance ; Immunity, Mucosal - immunology ; Immunization ; Intestines - immunology ; Intestines - pathology ; Mice ; Mice, Inbred C57BL ; Oral tolerance ; Ovalbumin ; Ovalbumin - administration & dosage ; Ovalbumin - immunology ; Peanuts ; Time Factors</subject><ispartof>Immunobiology (1979), 2009-04, Vol.214 (4), p.269-278</ispartof><rights>Elsevier GmbH</rights><rights>2008 Elsevier GmbH</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c443t-8e01e2d20afd9300e66887285e0f1ca0b09a9702208e6985234cf4669d515c193</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.imbio.2008.09.007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19327544$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Paschoal, Patrícia Olaya</creatorcontrib><creatorcontrib>Campos, Sylvia M.N</creatorcontrib><creatorcontrib>Pedruzzi, Monique M.B</creatorcontrib><creatorcontrib>Garrido, Valéria</creatorcontrib><creatorcontrib>Bisso, Mônica</creatorcontrib><creatorcontrib>Antunes, Danielle M.F</creatorcontrib><creatorcontrib>Nobrega, Alberto F</creatorcontrib><creatorcontrib>Teixeira, Gerlinde</creatorcontrib><title>Food allergy/hypersensitivity: Antigenicity or timing?</title><title>Immunobiology (1979)</title><addtitle>Immunobiology</addtitle><description>Abstract Mechanisms involved in the induction of oral tolerance (OT) or systemic immunization through the oral rout are still poorly understood. In our previous studies, we have shown that when normal mice eat peanuts they become tolerant, with no gut alterations. Conversely, if immunized with peanut proteins prior to a challenge diet (CD) containing peanuts they develop chronic inflammation of the gut. Our aim is to evaluate the consequences of the introduction of a novel protein in the diet of animals presenting antigen-specific gut inflammation. Adult, female C57BL/6J mice were divided in control (C) and experimental (E) groups. C1–C3 received peanut protein immunization, animals of the control groups C4 were sham immunized, and control group C5 received ovalbumin (OVA) immunization. The experimental group was immunized with peanut protein extract. Before initial exposure to a 30-day peanut containing CD, the experimental group was divided into 5 groups (E1–E5). OVA feeding began 7 days prior CD (E1) on day 0 (E2), 7 (E3), 14 (E4) and 21 (E5) during CD. Our results show that oral exposure to a novel protein (OVA) in the absence of gut inflammation (E1) leads to low levels of systemic antibody (Ab) titers, comparable to tolerant animals. Conversely, as off initial induction of inflammation, groups submitted to OVA (OT) protocol develop increasingly higher systemic Ab titers similar to animals of the immune control group. In conclusion, our protocol indicates that timing is more important than the antigenicity when a novel protein is offered, in the diet.</description><subject>Administration, Oral</subject><subject>Advanced Basic Science</subject><subject>Allergy and Immunology</subject><subject>Animals</subject><subject>Antibody Formation</subject><subject>Antigens, Plant - administration & dosage</subject><subject>Antigens, Plant - immunology</subject><subject>Arachis - immunology</subject><subject>Arachis hypogaea</subject><subject>Diet</subject><subject>Epitopes</subject><subject>Female</subject><subject>Food allergy</subject><subject>Food Hypersensitivity - immunology</subject><subject>Food Hypersensitivity - physiopathology</subject><subject>Humans</subject><subject>Immune Tolerance</subject><subject>Immunity, Mucosal - immunology</subject><subject>Immunization</subject><subject>Intestines - immunology</subject><subject>Intestines - pathology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Oral tolerance</subject><subject>Ovalbumin</subject><subject>Ovalbumin - administration & dosage</subject><subject>Ovalbumin - immunology</subject><subject>Peanuts</subject><subject>Time Factors</subject><issn>0171-2985</issn><issn>1878-3279</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkV1LwzAUhoMobn78AkF25V3rSdrmQ1ARcSoIXqjXoUtPZ2bbzKQT-u_N3EDwxqtw4HlPDs9LyAmFlALl54vUtjPrUgYgU1ApgNghYyqFTDIm1C4ZAxU0YUoWI3IQwgKAKibkPhlRFYkiz8eET52rJmXToJ8P5-_DEn3ALtjeftl-uJjcdL2dY2dNnCbOT3rb2m5-fUT26rIJeLx9D8nb9O719iF5er5_vL15SkyeZ30iESiyikFZVyoDQM6lFEwWCDU1JcxAlUoAYyCRxztZlps651xVBS1MvPKQnG32Lr37XGHodWuDwaYpO3SroLkAIVTO_wUZFBkoISKYbUDjXQgea730ti39oCnotVe90D9e9dqrBqWj15g63a5fzVqsfjNbkRG43AAYbXxZ9DoYi53Byno0va6c_eeDqz9509iovWw-cMCwcCvfRdGa6sA06Jd1tetmQcZWmYLsG7BknV4</recordid><startdate>20090401</startdate><enddate>20090401</enddate><creator>Paschoal, Patrícia Olaya</creator><creator>Campos, Sylvia M.N</creator><creator>Pedruzzi, Monique M.B</creator><creator>Garrido, Valéria</creator><creator>Bisso, Mônica</creator><creator>Antunes, Danielle M.F</creator><creator>Nobrega, Alberto F</creator><creator>Teixeira, Gerlinde</creator><general>Elsevier GmbH</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20090401</creationdate><title>Food allergy/hypersensitivity: Antigenicity or timing?</title><author>Paschoal, Patrícia Olaya ; Campos, Sylvia M.N ; Pedruzzi, Monique M.B ; Garrido, Valéria ; Bisso, Mônica ; Antunes, Danielle M.F ; Nobrega, Alberto F ; Teixeira, Gerlinde</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c443t-8e01e2d20afd9300e66887285e0f1ca0b09a9702208e6985234cf4669d515c193</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Administration, Oral</topic><topic>Advanced Basic Science</topic><topic>Allergy and Immunology</topic><topic>Animals</topic><topic>Antibody Formation</topic><topic>Antigens, Plant - administration & dosage</topic><topic>Antigens, Plant - immunology</topic><topic>Arachis - immunology</topic><topic>Arachis hypogaea</topic><topic>Diet</topic><topic>Epitopes</topic><topic>Female</topic><topic>Food allergy</topic><topic>Food Hypersensitivity - immunology</topic><topic>Food Hypersensitivity - physiopathology</topic><topic>Humans</topic><topic>Immune Tolerance</topic><topic>Immunity, Mucosal - immunology</topic><topic>Immunization</topic><topic>Intestines - immunology</topic><topic>Intestines - pathology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Oral tolerance</topic><topic>Ovalbumin</topic><topic>Ovalbumin - administration & dosage</topic><topic>Ovalbumin - immunology</topic><topic>Peanuts</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Paschoal, Patrícia Olaya</creatorcontrib><creatorcontrib>Campos, Sylvia M.N</creatorcontrib><creatorcontrib>Pedruzzi, Monique M.B</creatorcontrib><creatorcontrib>Garrido, Valéria</creatorcontrib><creatorcontrib>Bisso, Mônica</creatorcontrib><creatorcontrib>Antunes, Danielle M.F</creatorcontrib><creatorcontrib>Nobrega, Alberto F</creatorcontrib><creatorcontrib>Teixeira, Gerlinde</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Immunobiology (1979)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Paschoal, Patrícia Olaya</au><au>Campos, Sylvia M.N</au><au>Pedruzzi, Monique M.B</au><au>Garrido, Valéria</au><au>Bisso, Mônica</au><au>Antunes, Danielle M.F</au><au>Nobrega, Alberto F</au><au>Teixeira, Gerlinde</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Food allergy/hypersensitivity: Antigenicity or timing?</atitle><jtitle>Immunobiology (1979)</jtitle><addtitle>Immunobiology</addtitle><date>2009-04-01</date><risdate>2009</risdate><volume>214</volume><issue>4</issue><spage>269</spage><epage>278</epage><pages>269-278</pages><issn>0171-2985</issn><eissn>1878-3279</eissn><abstract>Abstract Mechanisms involved in the induction of oral tolerance (OT) or systemic immunization through the oral rout are still poorly understood. In our previous studies, we have shown that when normal mice eat peanuts they become tolerant, with no gut alterations. Conversely, if immunized with peanut proteins prior to a challenge diet (CD) containing peanuts they develop chronic inflammation of the gut. Our aim is to evaluate the consequences of the introduction of a novel protein in the diet of animals presenting antigen-specific gut inflammation. Adult, female C57BL/6J mice were divided in control (C) and experimental (E) groups. C1–C3 received peanut protein immunization, animals of the control groups C4 were sham immunized, and control group C5 received ovalbumin (OVA) immunization. The experimental group was immunized with peanut protein extract. Before initial exposure to a 30-day peanut containing CD, the experimental group was divided into 5 groups (E1–E5). OVA feeding began 7 days prior CD (E1) on day 0 (E2), 7 (E3), 14 (E4) and 21 (E5) during CD. Our results show that oral exposure to a novel protein (OVA) in the absence of gut inflammation (E1) leads to low levels of systemic antibody (Ab) titers, comparable to tolerant animals. Conversely, as off initial induction of inflammation, groups submitted to OVA (OT) protocol develop increasingly higher systemic Ab titers similar to animals of the immune control group. In conclusion, our protocol indicates that timing is more important than the antigenicity when a novel protein is offered, in the diet.</abstract><cop>Netherlands</cop><pub>Elsevier GmbH</pub><pmid>19327544</pmid><doi>10.1016/j.imbio.2008.09.007</doi><tpages>10</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0171-2985 |
ispartof | Immunobiology (1979), 2009-04, Vol.214 (4), p.269-278 |
issn | 0171-2985 1878-3279 |
language | eng |
recordid | cdi_proquest_miscellaneous_67077946 |
source | MEDLINE; Access via ScienceDirect (Elsevier) |
subjects | Administration, Oral Advanced Basic Science Allergy and Immunology Animals Antibody Formation Antigens, Plant - administration & dosage Antigens, Plant - immunology Arachis - immunology Arachis hypogaea Diet Epitopes Female Food allergy Food Hypersensitivity - immunology Food Hypersensitivity - physiopathology Humans Immune Tolerance Immunity, Mucosal - immunology Immunization Intestines - immunology Intestines - pathology Mice Mice, Inbred C57BL Oral tolerance Ovalbumin Ovalbumin - administration & dosage Ovalbumin - immunology Peanuts Time Factors |
title | Food allergy/hypersensitivity: Antigenicity or timing? |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T00%3A53%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Food%20allergy/hypersensitivity:%20Antigenicity%20or%20timing?&rft.jtitle=Immunobiology%20(1979)&rft.au=Paschoal,%20Patr%C3%ADcia%20Olaya&rft.date=2009-04-01&rft.volume=214&rft.issue=4&rft.spage=269&rft.epage=278&rft.pages=269-278&rft.issn=0171-2985&rft.eissn=1878-3279&rft_id=info:doi/10.1016/j.imbio.2008.09.007&rft_dat=%3Cproquest_cross%3E20530977%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=20530977&rft_id=info:pmid/19327544&rft_els_id=1_s2_0_S0171298508001290&rfr_iscdi=true |