Radio-protective effect of vitamin E on spermatogenesis in mice exposed to gamma-irradiation: a flow cytometric study
To investigate the effect of vitamin E on the radioprotection of spermatogenesis and chromatin condensation of spermatozoa during passage through the epididymis in mice exposed to irradiation. Adult outbred male ICR mice were orally administered natural vitamin E (VE, D-alpha-tocopheryl acetate) at...
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Veröffentlicht in: | Asian journal of andrology 2004-12, Vol.6 (4), p.331-336 |
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creator | Songthaveesin, C Saikhun, J Kitiyanant, Y Pavasuthipaisit, K |
description | To investigate the effect of vitamin E on the radioprotection of spermatogenesis and chromatin condensation of spermatozoa during passage through the epididymis in mice exposed to irradiation.
Adult outbred male ICR mice were orally administered natural vitamin E (VE, D-alpha-tocopheryl acetate) at 400 IU/kg for 7 days before exposure to 1 Gy of gamma-irradiation. The animals were sacrificed at day 1, 7, 14, 21, 28, 35 and 70 post-irradiation (IR) and the percentage of testicular germ cells and epididymal sperm chromatin condensation was analyzed using flow cytometry.
Serum D-alpha-tocopheryl acetate levels were 47.4+/-3.2 microg/dL in the treated group, yet it could not be detected in the control group. The testicular weight of irradiated mice pretreated with VE+IR was significantly (P |
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Adult outbred male ICR mice were orally administered natural vitamin E (VE, D-alpha-tocopheryl acetate) at 400 IU/kg for 7 days before exposure to 1 Gy of gamma-irradiation. The animals were sacrificed at day 1, 7, 14, 21, 28, 35 and 70 post-irradiation (IR) and the percentage of testicular germ cells and epididymal sperm chromatin condensation was analyzed using flow cytometry.
Serum D-alpha-tocopheryl acetate levels were 47.4+/-3.2 microg/dL in the treated group, yet it could not be detected in the control group. The testicular weight of irradiated mice pretreated with VE+IR was significantly (P<0.05) higher than that of those without VE treatment (IR) at day 14 and 21 post-irradiation. The percentage of primary spermatocytes (4C) in the VE+IR group was comparable to the controls but significantly (P<0.05) higher than those in the IR group from day 7 to 35 post-irradiation. The percentage of round spermatids (1C) in the VE+IR group was also significantly (P<0.05) higher than those in the IR group at day 28 post-irradiation. The primary spermatocytes : spermatogonia ratio in the IR group was significantly (P<0.05) declined at day 7 to 35 post-irradiation when compared to the VE+IR and control groups. The round spermatid : spermatogonia ratio in the VE+IR group was significantly (P<0.05) higher than that of the IR group at day 14 and 28 post-irradiation. The chromatin condensation of epididymal spermatozoa measured by propidium iodide uptake was not affected by 1 Gy of gamma-irradiation.
The administration of VE prior to irradiation protects spermatogenic cells from radiation.</description><identifier>ISSN: 1008-682X</identifier><identifier>PMID: 15546025</identifier><language>eng</language><publisher>China</publisher><subject>Animals ; Antioxidants - pharmacology ; Chromatin - drug effects ; Chromatin - radiation effects ; Flow Cytometry ; Gamma Rays ; Germ Cells - drug effects ; Male ; Mice ; Mice, Inbred ICR ; Organ Size - drug effects ; Propidium ; Radiation-Protective Agents ; Spermatids - drug effects ; Spermatids - radiation effects ; Spermatogenesis - drug effects ; Spermatogenesis - radiation effects ; Testis - cytology ; Testis - drug effects ; Testis - radiation effects ; Vitamin E - blood ; Vitamin E - pharmacology</subject><ispartof>Asian journal of andrology, 2004-12, Vol.6 (4), p.331-336</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15546025$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Songthaveesin, C</creatorcontrib><creatorcontrib>Saikhun, J</creatorcontrib><creatorcontrib>Kitiyanant, Y</creatorcontrib><creatorcontrib>Pavasuthipaisit, K</creatorcontrib><title>Radio-protective effect of vitamin E on spermatogenesis in mice exposed to gamma-irradiation: a flow cytometric study</title><title>Asian journal of andrology</title><addtitle>Asian J Androl</addtitle><description>To investigate the effect of vitamin E on the radioprotection of spermatogenesis and chromatin condensation of spermatozoa during passage through the epididymis in mice exposed to irradiation.
Adult outbred male ICR mice were orally administered natural vitamin E (VE, D-alpha-tocopheryl acetate) at 400 IU/kg for 7 days before exposure to 1 Gy of gamma-irradiation. The animals were sacrificed at day 1, 7, 14, 21, 28, 35 and 70 post-irradiation (IR) and the percentage of testicular germ cells and epididymal sperm chromatin condensation was analyzed using flow cytometry.
Serum D-alpha-tocopheryl acetate levels were 47.4+/-3.2 microg/dL in the treated group, yet it could not be detected in the control group. The testicular weight of irradiated mice pretreated with VE+IR was significantly (P<0.05) higher than that of those without VE treatment (IR) at day 14 and 21 post-irradiation. The percentage of primary spermatocytes (4C) in the VE+IR group was comparable to the controls but significantly (P<0.05) higher than those in the IR group from day 7 to 35 post-irradiation. The percentage of round spermatids (1C) in the VE+IR group was also significantly (P<0.05) higher than those in the IR group at day 28 post-irradiation. The primary spermatocytes : spermatogonia ratio in the IR group was significantly (P<0.05) declined at day 7 to 35 post-irradiation when compared to the VE+IR and control groups. The round spermatid : spermatogonia ratio in the VE+IR group was significantly (P<0.05) higher than that of the IR group at day 14 and 28 post-irradiation. The chromatin condensation of epididymal spermatozoa measured by propidium iodide uptake was not affected by 1 Gy of gamma-irradiation.
The administration of VE prior to irradiation protects spermatogenic cells from radiation.</description><subject>Animals</subject><subject>Antioxidants - pharmacology</subject><subject>Chromatin - drug effects</subject><subject>Chromatin - radiation effects</subject><subject>Flow Cytometry</subject><subject>Gamma Rays</subject><subject>Germ Cells - drug effects</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred ICR</subject><subject>Organ Size - drug effects</subject><subject>Propidium</subject><subject>Radiation-Protective Agents</subject><subject>Spermatids - drug effects</subject><subject>Spermatids - radiation effects</subject><subject>Spermatogenesis - drug effects</subject><subject>Spermatogenesis - radiation effects</subject><subject>Testis - cytology</subject><subject>Testis - drug effects</subject><subject>Testis - radiation effects</subject><subject>Vitamin E - blood</subject><subject>Vitamin E - pharmacology</subject><issn>1008-682X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo1kMtKxDAUhrNQnHH0FSQrd4Vcml7cyTBeYEAQBXclTU6GSNPUJB3t2xtwXJ3D4fs_OP8ZWlNCmqJq2McKXcb4SQjjtG0v0IoKUVaEiTWaX6W2vpiCT6CSPQIGY_KGvcFHm6SzI95hP-I4QXAy-QOMEG3E-e6syvjP5CNonDw-SOdkYUPISpmsH--wxGbw31gtyTtIwSoc06yXK3Ru5BDh-jQ36P1h97Z9KvYvj8_b-30xMdKmou4pAaVpX2tDTV_2rCKlIdwAiJIT3hLFGqCi5rwpVQtc8ZqIhoFWggAYvkG3f97839cMMXXORgXDIEfwc-yqmuRsxTJ4cwLn3oHupmCdDEv3XxT_BTQsZck</recordid><startdate>200412</startdate><enddate>200412</enddate><creator>Songthaveesin, C</creator><creator>Saikhun, J</creator><creator>Kitiyanant, Y</creator><creator>Pavasuthipaisit, K</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>200412</creationdate><title>Radio-protective effect of vitamin E on spermatogenesis in mice exposed to gamma-irradiation: a flow cytometric study</title><author>Songthaveesin, C ; Saikhun, J ; Kitiyanant, Y ; Pavasuthipaisit, K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p209t-7b10ecd1b7df1fb4b2604f03fee5430390c28e1573384c9e3c370582edc50eef3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Antioxidants - pharmacology</topic><topic>Chromatin - drug effects</topic><topic>Chromatin - radiation effects</topic><topic>Flow Cytometry</topic><topic>Gamma Rays</topic><topic>Germ Cells - drug effects</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred ICR</topic><topic>Organ Size - drug effects</topic><topic>Propidium</topic><topic>Radiation-Protective Agents</topic><topic>Spermatids - drug effects</topic><topic>Spermatids - radiation effects</topic><topic>Spermatogenesis - drug effects</topic><topic>Spermatogenesis - radiation effects</topic><topic>Testis - cytology</topic><topic>Testis - drug effects</topic><topic>Testis - radiation effects</topic><topic>Vitamin E - blood</topic><topic>Vitamin E - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Songthaveesin, C</creatorcontrib><creatorcontrib>Saikhun, J</creatorcontrib><creatorcontrib>Kitiyanant, Y</creatorcontrib><creatorcontrib>Pavasuthipaisit, K</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Asian journal of andrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Songthaveesin, C</au><au>Saikhun, J</au><au>Kitiyanant, Y</au><au>Pavasuthipaisit, K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Radio-protective effect of vitamin E on spermatogenesis in mice exposed to gamma-irradiation: a flow cytometric study</atitle><jtitle>Asian journal of andrology</jtitle><addtitle>Asian J Androl</addtitle><date>2004-12</date><risdate>2004</risdate><volume>6</volume><issue>4</issue><spage>331</spage><epage>336</epage><pages>331-336</pages><issn>1008-682X</issn><abstract>To investigate the effect of vitamin E on the radioprotection of spermatogenesis and chromatin condensation of spermatozoa during passage through the epididymis in mice exposed to irradiation.
Adult outbred male ICR mice were orally administered natural vitamin E (VE, D-alpha-tocopheryl acetate) at 400 IU/kg for 7 days before exposure to 1 Gy of gamma-irradiation. The animals were sacrificed at day 1, 7, 14, 21, 28, 35 and 70 post-irradiation (IR) and the percentage of testicular germ cells and epididymal sperm chromatin condensation was analyzed using flow cytometry.
Serum D-alpha-tocopheryl acetate levels were 47.4+/-3.2 microg/dL in the treated group, yet it could not be detected in the control group. The testicular weight of irradiated mice pretreated with VE+IR was significantly (P<0.05) higher than that of those without VE treatment (IR) at day 14 and 21 post-irradiation. The percentage of primary spermatocytes (4C) in the VE+IR group was comparable to the controls but significantly (P<0.05) higher than those in the IR group from day 7 to 35 post-irradiation. The percentage of round spermatids (1C) in the VE+IR group was also significantly (P<0.05) higher than those in the IR group at day 28 post-irradiation. The primary spermatocytes : spermatogonia ratio in the IR group was significantly (P<0.05) declined at day 7 to 35 post-irradiation when compared to the VE+IR and control groups. The round spermatid : spermatogonia ratio in the VE+IR group was significantly (P<0.05) higher than that of the IR group at day 14 and 28 post-irradiation. The chromatin condensation of epididymal spermatozoa measured by propidium iodide uptake was not affected by 1 Gy of gamma-irradiation.
The administration of VE prior to irradiation protects spermatogenic cells from radiation.</abstract><cop>China</cop><pmid>15546025</pmid><tpages>6</tpages></addata></record> |
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subjects | Animals Antioxidants - pharmacology Chromatin - drug effects Chromatin - radiation effects Flow Cytometry Gamma Rays Germ Cells - drug effects Male Mice Mice, Inbred ICR Organ Size - drug effects Propidium Radiation-Protective Agents Spermatids - drug effects Spermatids - radiation effects Spermatogenesis - drug effects Spermatogenesis - radiation effects Testis - cytology Testis - drug effects Testis - radiation effects Vitamin E - blood Vitamin E - pharmacology |
title | Radio-protective effect of vitamin E on spermatogenesis in mice exposed to gamma-irradiation: a flow cytometric study |
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