Processing of pro-thrombostasin by a recombinant subtilisin-like proprotein convertase derived from the salivary glands of horn flies ( Haematobia irritans)
Thrombostasin (TS) is a thrombin inhibitor found in the salivary glands of horn flies ( Haematobia irritans). It is produced as an inactive form with a 76-amino acid propeptide in the N-terminus preceding the mature TS. A minimal recognition sequence by subtilisin-like proprotein convertases, Arg-Xa...
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| Veröffentlicht in: | Insect biochemistry and molecular biology 2004-12, Vol.34 (12), p.1289-1295 |
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| container_title | Insect biochemistry and molecular biology |
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| creator | Zhang, Dunhua Cupp, Mary S. Cupp, Ed W. |
| description | Thrombostasin (TS) is a thrombin inhibitor found in the salivary glands of horn flies (
Haematobia irritans). It is produced as an inactive form with a 76-amino acid propeptide in the N-terminus preceding the mature TS. A minimal recognition sequence by subtilisin-like proprotein convertases, Arg-Xaa-Xaa-Arg, is localized C-terminal to the propeptide. This study demonstrated that a gene cloned from the salivary glands of the horn fly encodes a new convertase, subsequently named horn fly proprotein convertase (HFPC), and that the recombinant HFPC expressed in insect HighFive cell culture specifically cleaves recombinant pro-thrombostasin, produced in
E. coli, at the expected site. The relative cleavage efficiency of rHFPC was compared with that of recombinant human furin, a commercially available proprotein convertase. The result indicated that this newly identified proprotein convertase is of importance for the proteolytic maturation of thrombostasin, a protein secreted in horn fly saliva and used by the insect to counteract its host's haemostatic response. |
| doi_str_mv | 10.1016/j.ibmb.2004.08.007 |
| format | Article |
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Haematobia irritans). It is produced as an inactive form with a 76-amino acid propeptide in the N-terminus preceding the mature TS. A minimal recognition sequence by subtilisin-like proprotein convertases, Arg-Xaa-Xaa-Arg, is localized C-terminal to the propeptide. This study demonstrated that a gene cloned from the salivary glands of the horn fly encodes a new convertase, subsequently named horn fly proprotein convertase (HFPC), and that the recombinant HFPC expressed in insect HighFive cell culture specifically cleaves recombinant pro-thrombostasin, produced in
E. coli, at the expected site. The relative cleavage efficiency of rHFPC was compared with that of recombinant human furin, a commercially available proprotein convertase. The result indicated that this newly identified proprotein convertase is of importance for the proteolytic maturation of thrombostasin, a protein secreted in horn fly saliva and used by the insect to counteract its host's haemostatic response.</description><identifier>ISSN: 0965-1748</identifier><identifier>EISSN: 1879-0240</identifier><identifier>DOI: 10.1016/j.ibmb.2004.08.007</identifier><identifier>PMID: 15544942</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Amino Acid Sequence ; amino acid sequences ; Animals ; Base Sequence ; Cloning, Molecular ; complementary DNA ; Escherichia coli - genetics ; gene expression ; Haematobia irritans ; HFPC ; Horn fly ; Insect Proteins - metabolism ; Molecular Sequence Data ; Muscidae ; Muscidae - enzymology ; Muscidae - genetics ; Muscidae - metabolism ; nucleotide sequences ; post-translational modification ; Proprotein Convertases - chemistry ; Proprotein Convertases - genetics ; Proprotein Convertases - metabolism ; protein cleavage ; Protein Precursors - metabolism ; proteinase inhibitors ; proteinases ; proteolysis ; proteolytic processing ; prothrombostasin ; recombinant proteins ; Recombinant Proteins - metabolism ; salivary glands ; Salivary Glands - enzymology ; Subtilisin-like proprotein convertase ; Thrombostasin</subject><ispartof>Insect biochemistry and molecular biology, 2004-12, Vol.34 (12), p.1289-1295</ispartof><rights>2004</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c407t-77d328d6a76c87bec06c9deffd176e7713f91ca7f2b893217160158919e0c3213</citedby><cites>FETCH-LOGICAL-c407t-77d328d6a76c87bec06c9deffd176e7713f91ca7f2b893217160158919e0c3213</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ibmb.2004.08.007$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15544942$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zhang, Dunhua</creatorcontrib><creatorcontrib>Cupp, Mary S.</creatorcontrib><creatorcontrib>Cupp, Ed W.</creatorcontrib><title>Processing of pro-thrombostasin by a recombinant subtilisin-like proprotein convertase derived from the salivary glands of horn flies ( Haematobia irritans)</title><title>Insect biochemistry and molecular biology</title><addtitle>Insect Biochem Mol Biol</addtitle><description>Thrombostasin (TS) is a thrombin inhibitor found in the salivary glands of horn flies (
Haematobia irritans). It is produced as an inactive form with a 76-amino acid propeptide in the N-terminus preceding the mature TS. A minimal recognition sequence by subtilisin-like proprotein convertases, Arg-Xaa-Xaa-Arg, is localized C-terminal to the propeptide. This study demonstrated that a gene cloned from the salivary glands of the horn fly encodes a new convertase, subsequently named horn fly proprotein convertase (HFPC), and that the recombinant HFPC expressed in insect HighFive cell culture specifically cleaves recombinant pro-thrombostasin, produced in
E. coli, at the expected site. The relative cleavage efficiency of rHFPC was compared with that of recombinant human furin, a commercially available proprotein convertase. The result indicated that this newly identified proprotein convertase is of importance for the proteolytic maturation of thrombostasin, a protein secreted in horn fly saliva and used by the insect to counteract its host's haemostatic response.</description><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>Escherichia coli - genetics</subject><subject>gene expression</subject><subject>Haematobia irritans</subject><subject>HFPC</subject><subject>Horn fly</subject><subject>Insect Proteins - metabolism</subject><subject>Molecular Sequence Data</subject><subject>Muscidae</subject><subject>Muscidae - enzymology</subject><subject>Muscidae - genetics</subject><subject>Muscidae - metabolism</subject><subject>nucleotide sequences</subject><subject>post-translational modification</subject><subject>Proprotein Convertases - chemistry</subject><subject>Proprotein Convertases - genetics</subject><subject>Proprotein Convertases - metabolism</subject><subject>protein cleavage</subject><subject>Protein Precursors - metabolism</subject><subject>proteinase inhibitors</subject><subject>proteinases</subject><subject>proteolysis</subject><subject>proteolytic processing</subject><subject>prothrombostasin</subject><subject>recombinant proteins</subject><subject>Recombinant Proteins - metabolism</subject><subject>salivary glands</subject><subject>Salivary Glands - enzymology</subject><subject>Subtilisin-like proprotein convertase</subject><subject>Thrombostasin</subject><issn>0965-1748</issn><issn>1879-0240</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc-KFDEQxoMo7rj6Ah40J9FDt5X-l27YiyzqCgsKuueQTiozGbs7a5IZ2HfxYa1mBrwpBEIqv-9LVT7GXgooBYju_b704zyWFUBTQl8CyEdsI3o5FFA18JhtYOjaQsimv2DPUtoDgU0rn7IL0bZNMzTVhv3-FoPBlPyy5cHx-xiKvIthHkPKmqp8fOCaRzRU8oteMk-HMfvJ010x-Z-4SmhlJNaE5YiRdMgtRn9Eyx158bxDnvTkjzo-8O2kF5vWx3YhLtxNHhN_y280zjqH0WvuY_RZL-ndc_bE6Snhi_N-ye4-ffxxfVPcfv385frDbWEakLmQ0tZVbzstO9PLEQ10ZrDonBWyQylF7QZhtHTV2A91JaToQLT9IAYEQ-f6kr05-dIgvw6Yspp9MjhRpxgOSXUSpBBC_hcUkj6b3AmsTqCJIaWITt1HP9P4SoBaw1N7tYan1vAU9IrCI9Grs_thnNH-lZzTIuD1CXA6KL2NPqm77xWIGijpqpU1EVcnAum7jh6jSsbjYtB6yjArG_y_OvgDNqW3JQ</recordid><startdate>20041201</startdate><enddate>20041201</enddate><creator>Zhang, Dunhua</creator><creator>Cupp, Mary S.</creator><creator>Cupp, Ed W.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>7X8</scope></search><sort><creationdate>20041201</creationdate><title>Processing of pro-thrombostasin by a recombinant subtilisin-like proprotein convertase derived from the salivary glands of horn flies ( Haematobia irritans)</title><author>Zhang, Dunhua ; Cupp, Mary S. ; Cupp, Ed W.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-77d328d6a76c87bec06c9deffd176e7713f91ca7f2b893217160158919e0c3213</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>Escherichia coli - genetics</topic><topic>gene expression</topic><topic>Haematobia irritans</topic><topic>HFPC</topic><topic>Horn fly</topic><topic>Insect Proteins - metabolism</topic><topic>Molecular Sequence Data</topic><topic>Muscidae</topic><topic>Muscidae - enzymology</topic><topic>Muscidae - genetics</topic><topic>Muscidae - metabolism</topic><topic>nucleotide sequences</topic><topic>post-translational modification</topic><topic>Proprotein Convertases - chemistry</topic><topic>Proprotein Convertases - genetics</topic><topic>Proprotein Convertases - metabolism</topic><topic>protein cleavage</topic><topic>Protein Precursors - metabolism</topic><topic>proteinase inhibitors</topic><topic>proteinases</topic><topic>proteolysis</topic><topic>proteolytic processing</topic><topic>prothrombostasin</topic><topic>recombinant proteins</topic><topic>Recombinant Proteins - metabolism</topic><topic>salivary glands</topic><topic>Salivary Glands - enzymology</topic><topic>Subtilisin-like proprotein convertase</topic><topic>Thrombostasin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zhang, Dunhua</creatorcontrib><creatorcontrib>Cupp, Mary S.</creatorcontrib><creatorcontrib>Cupp, Ed W.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>MEDLINE - Academic</collection><jtitle>Insect biochemistry and molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zhang, Dunhua</au><au>Cupp, Mary S.</au><au>Cupp, Ed W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Processing of pro-thrombostasin by a recombinant subtilisin-like proprotein convertase derived from the salivary glands of horn flies ( Haematobia irritans)</atitle><jtitle>Insect biochemistry and molecular biology</jtitle><addtitle>Insect Biochem Mol Biol</addtitle><date>2004-12-01</date><risdate>2004</risdate><volume>34</volume><issue>12</issue><spage>1289</spage><epage>1295</epage><pages>1289-1295</pages><issn>0965-1748</issn><eissn>1879-0240</eissn><abstract>Thrombostasin (TS) is a thrombin inhibitor found in the salivary glands of horn flies (
Haematobia irritans). It is produced as an inactive form with a 76-amino acid propeptide in the N-terminus preceding the mature TS. A minimal recognition sequence by subtilisin-like proprotein convertases, Arg-Xaa-Xaa-Arg, is localized C-terminal to the propeptide. This study demonstrated that a gene cloned from the salivary glands of the horn fly encodes a new convertase, subsequently named horn fly proprotein convertase (HFPC), and that the recombinant HFPC expressed in insect HighFive cell culture specifically cleaves recombinant pro-thrombostasin, produced in
E. coli, at the expected site. The relative cleavage efficiency of rHFPC was compared with that of recombinant human furin, a commercially available proprotein convertase. The result indicated that this newly identified proprotein convertase is of importance for the proteolytic maturation of thrombostasin, a protein secreted in horn fly saliva and used by the insect to counteract its host's haemostatic response.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>15544942</pmid><doi>10.1016/j.ibmb.2004.08.007</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Amino Acid Sequence amino acid sequences Animals Base Sequence Cloning, Molecular complementary DNA Escherichia coli - genetics gene expression Haematobia irritans HFPC Horn fly Insect Proteins - metabolism Molecular Sequence Data Muscidae Muscidae - enzymology Muscidae - genetics Muscidae - metabolism nucleotide sequences post-translational modification Proprotein Convertases - chemistry Proprotein Convertases - genetics Proprotein Convertases - metabolism protein cleavage Protein Precursors - metabolism proteinase inhibitors proteinases proteolysis proteolytic processing prothrombostasin recombinant proteins Recombinant Proteins - metabolism salivary glands Salivary Glands - enzymology Subtilisin-like proprotein convertase Thrombostasin |
| title | Processing of pro-thrombostasin by a recombinant subtilisin-like proprotein convertase derived from the salivary glands of horn flies ( Haematobia irritans) |
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