Identification and characterization of linear B-cell epitopes of β-globulin, a major allergen of sesame seeds
The increased consumption of foods containing sesame seeds is paralleled by an increase in reported sesame-induced allergic reactions. This study aimed at identifying and characterizing the linear B-cell epitopes of the 14-kd β-globulin, the major allergen of sesame seed. A peptide containing 71 ami...
Gespeichert in:
Veröffentlicht in: | Journal of Allergy and Clinical Immunology 2004-11, Vol.114 (5), p.1151-1158 |
---|---|
Hauptverfasser: | , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 1158 |
---|---|
container_issue | 5 |
container_start_page | 1151 |
container_title | Journal of Allergy and Clinical Immunology |
container_volume | 114 |
creator | Wolff, Neomi Yannai, Shmuel Karin, Nathan Levy, Yael Reifen, Ram Dalal, Ilan Cogan, Uri |
description | The increased consumption of foods containing sesame seeds is paralleled by an increase in reported sesame-induced allergic reactions.
This study aimed at identifying and characterizing the linear B-cell epitopes of the 14-kd β-globulin, the major allergen of sesame seed.
A peptide containing 71 amino acids (peptide B) was previously identified by us as the IgE-binding region on β-globulin. To determine the amino acid sequence of the IgE-binding sites on peptide B, we synthesized overlapping peptides 20 and 10 amino acid residues long that span the entire length of peptide B, which were offset from each other by 10 and 2 amino acid residues, respectively. Sera from 20 subjects given diagnoses of allergy to sesame β-globulin served to identify the epitopes by using the dot-blot test.
At least 9 different IgE-recognition sites were identified on peptide B. Three of them, numbers 2, 3, and 13 (corresponding to amino acids 46-55, 48-57, and 76-86, respectively, in the β-globulin sequence), appeared to be immunodominant IgE-binding epitopes. Also, these peptides were best recognized in terms of intensity of response. There was no obvious sequence motif shared by the 9 different IgE-binding epitopes of β-globulin. However, approximately 60% of the amino acids represented in the epitopes are hydrophobic residues.
Identification of the IgE-binding epitopes might provide a better understanding of the functional role the allergens play in the disease and might have implications for immunodiagnosis and probably immunotherapy. |
doi_str_mv | 10.1016/j.jaci.2004.07.038 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67068568</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0091674904020834</els_id><sourcerecordid>67068568</sourcerecordid><originalsourceid>FETCH-LOGICAL-c413t-36d064f9da03daa186769c588544c3f61d0da32ad765946d7277f2c679ef5f503</originalsourceid><addsrcrecordid>eNqFkU2O1DAQhS0EYpqGC7BA2cCKhHLs2InEBkb8jDQSG1hbNXZ5cJTEjZ1GgmNxEM6EQ7c0O1iVqup7pdJ7jD3l0HDg6tXYjGhD0wLIBnQDor_HdhwGXau-7e6zHcDAa6XlcMEe5TxC6UU_PGQXvOuEkq3cseXK0bIGHyyuIS4VLq6yXzGhXSmFn6dh9NUUFsJUva0tTVNFh7DGA-Vt8_tXfTvFm2MhXlZYzTjGVOE0Ubqlv9JMGWcqhVx-zB54nDI9Odc9-_L-3efLj_X1pw9Xl2-uayu5WGuhHCjpB4cgHCLvlVaD7fq-k9IKr7gDh6JFp1U3SOV0q7VvrdID-c53IPbsxenuIcVvR8qrmUPeXseF4jEbpUH1ner_C_JB9RKgLWB7Am2KOSfy5pDCjOmH4WC2OMxotjjMFocBbUocRfTsfP14M5O7k5z9L8DzM4DZ4uQTLjbkO061GmSxZM9enzgqpn0PlEy2gRZLLiSyq3Ex_OuPPxNzqR4</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>19684002</pqid></control><display><type>article</type><title>Identification and characterization of linear B-cell epitopes of β-globulin, a major allergen of sesame seeds</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><source>IngentaConnect Free/Open Access Journals</source><source>Access via ScienceDirect (Elsevier)</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Wiley Online Library (Open Access Collection)</source><source>PubMed Central</source><creator>Wolff, Neomi ; Yannai, Shmuel ; Karin, Nathan ; Levy, Yael ; Reifen, Ram ; Dalal, Ilan ; Cogan, Uri</creator><creatorcontrib>Wolff, Neomi ; Yannai, Shmuel ; Karin, Nathan ; Levy, Yael ; Reifen, Ram ; Dalal, Ilan ; Cogan, Uri</creatorcontrib><description>The increased consumption of foods containing sesame seeds is paralleled by an increase in reported sesame-induced allergic reactions.
This study aimed at identifying and characterizing the linear B-cell epitopes of the 14-kd β-globulin, the major allergen of sesame seed.
A peptide containing 71 amino acids (peptide B) was previously identified by us as the IgE-binding region on β-globulin. To determine the amino acid sequence of the IgE-binding sites on peptide B, we synthesized overlapping peptides 20 and 10 amino acid residues long that span the entire length of peptide B, which were offset from each other by 10 and 2 amino acid residues, respectively. Sera from 20 subjects given diagnoses of allergy to sesame β-globulin served to identify the epitopes by using the dot-blot test.
At least 9 different IgE-recognition sites were identified on peptide B. Three of them, numbers 2, 3, and 13 (corresponding to amino acids 46-55, 48-57, and 76-86, respectively, in the β-globulin sequence), appeared to be immunodominant IgE-binding epitopes. Also, these peptides were best recognized in terms of intensity of response. There was no obvious sequence motif shared by the 9 different IgE-binding epitopes of β-globulin. However, approximately 60% of the amino acids represented in the epitopes are hydrophobic residues.
Identification of the IgE-binding epitopes might provide a better understanding of the functional role the allergens play in the disease and might have implications for immunodiagnosis and probably immunotherapy.</description><identifier>ISSN: 0091-6749</identifier><identifier>EISSN: 1097-6825</identifier><identifier>EISSN: 1365-2567</identifier><identifier>DOI: 10.1016/j.jaci.2004.07.038</identifier><identifier>PMID: 15536424</identifier><identifier>CODEN: JACIBY</identifier><language>eng</language><publisher>New York, NY: Mosby, Inc</publisher><subject>Adolescent ; Adult ; Allergenicity ; Allergens - immunology ; Amino Acid Sequence ; Beta-Globulins - immunology ; Biological and medical sciences ; Child ; Child, Preschool ; epitopes ; Epitopes, B-Lymphocyte ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Humans ; IgE ; Immunoglobulin E - immunology ; Immunopathology ; Infant ; major allergen ; Medical sciences ; Molecular Sequence Data ; peptide synthesis ; Seeds - immunology ; sesame seeds ; Sesamum - immunology</subject><ispartof>Journal of Allergy and Clinical Immunology, 2004-11, Vol.114 (5), p.1151-1158</ispartof><rights>2004 American Academy of Allergy, Asthma and Immunology</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-36d064f9da03daa186769c588544c3f61d0da32ad765946d7277f2c679ef5f503</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jaci.2004.07.038$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16270441$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15536424$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolff, Neomi</creatorcontrib><creatorcontrib>Yannai, Shmuel</creatorcontrib><creatorcontrib>Karin, Nathan</creatorcontrib><creatorcontrib>Levy, Yael</creatorcontrib><creatorcontrib>Reifen, Ram</creatorcontrib><creatorcontrib>Dalal, Ilan</creatorcontrib><creatorcontrib>Cogan, Uri</creatorcontrib><title>Identification and characterization of linear B-cell epitopes of β-globulin, a major allergen of sesame seeds</title><title>Journal of Allergy and Clinical Immunology</title><addtitle>J Allergy Clin Immunol</addtitle><description>The increased consumption of foods containing sesame seeds is paralleled by an increase in reported sesame-induced allergic reactions.
This study aimed at identifying and characterizing the linear B-cell epitopes of the 14-kd β-globulin, the major allergen of sesame seed.
A peptide containing 71 amino acids (peptide B) was previously identified by us as the IgE-binding region on β-globulin. To determine the amino acid sequence of the IgE-binding sites on peptide B, we synthesized overlapping peptides 20 and 10 amino acid residues long that span the entire length of peptide B, which were offset from each other by 10 and 2 amino acid residues, respectively. Sera from 20 subjects given diagnoses of allergy to sesame β-globulin served to identify the epitopes by using the dot-blot test.
At least 9 different IgE-recognition sites were identified on peptide B. Three of them, numbers 2, 3, and 13 (corresponding to amino acids 46-55, 48-57, and 76-86, respectively, in the β-globulin sequence), appeared to be immunodominant IgE-binding epitopes. Also, these peptides were best recognized in terms of intensity of response. There was no obvious sequence motif shared by the 9 different IgE-binding epitopes of β-globulin. However, approximately 60% of the amino acids represented in the epitopes are hydrophobic residues.
Identification of the IgE-binding epitopes might provide a better understanding of the functional role the allergens play in the disease and might have implications for immunodiagnosis and probably immunotherapy.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Allergenicity</subject><subject>Allergens - immunology</subject><subject>Amino Acid Sequence</subject><subject>Beta-Globulins - immunology</subject><subject>Biological and medical sciences</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>epitopes</subject><subject>Epitopes, B-Lymphocyte</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>IgE</subject><subject>Immunoglobulin E - immunology</subject><subject>Immunopathology</subject><subject>Infant</subject><subject>major allergen</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>peptide synthesis</subject><subject>Seeds - immunology</subject><subject>sesame seeds</subject><subject>Sesamum - immunology</subject><issn>0091-6749</issn><issn>1097-6825</issn><issn>1365-2567</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU2O1DAQhS0EYpqGC7BA2cCKhHLs2InEBkb8jDQSG1hbNXZ5cJTEjZ1GgmNxEM6EQ7c0O1iVqup7pdJ7jD3l0HDg6tXYjGhD0wLIBnQDor_HdhwGXau-7e6zHcDAa6XlcMEe5TxC6UU_PGQXvOuEkq3cseXK0bIGHyyuIS4VLq6yXzGhXSmFn6dh9NUUFsJUva0tTVNFh7DGA-Vt8_tXfTvFm2MhXlZYzTjGVOE0Ubqlv9JMGWcqhVx-zB54nDI9Odc9-_L-3efLj_X1pw9Xl2-uayu5WGuhHCjpB4cgHCLvlVaD7fq-k9IKr7gDh6JFp1U3SOV0q7VvrdID-c53IPbsxenuIcVvR8qrmUPeXseF4jEbpUH1ner_C_JB9RKgLWB7Am2KOSfy5pDCjOmH4WC2OMxotjjMFocBbUocRfTsfP14M5O7k5z9L8DzM4DZ4uQTLjbkO061GmSxZM9enzgqpn0PlEy2gRZLLiSyq3Ex_OuPPxNzqR4</recordid><startdate>20041101</startdate><enddate>20041101</enddate><creator>Wolff, Neomi</creator><creator>Yannai, Shmuel</creator><creator>Karin, Nathan</creator><creator>Levy, Yael</creator><creator>Reifen, Ram</creator><creator>Dalal, Ilan</creator><creator>Cogan, Uri</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20041101</creationdate><title>Identification and characterization of linear B-cell epitopes of β-globulin, a major allergen of sesame seeds</title><author>Wolff, Neomi ; Yannai, Shmuel ; Karin, Nathan ; Levy, Yael ; Reifen, Ram ; Dalal, Ilan ; Cogan, Uri</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c413t-36d064f9da03daa186769c588544c3f61d0da32ad765946d7277f2c679ef5f503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Allergenicity</topic><topic>Allergens - immunology</topic><topic>Amino Acid Sequence</topic><topic>Beta-Globulins - immunology</topic><topic>Biological and medical sciences</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>epitopes</topic><topic>Epitopes, B-Lymphocyte</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>IgE</topic><topic>Immunoglobulin E - immunology</topic><topic>Immunopathology</topic><topic>Infant</topic><topic>major allergen</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>peptide synthesis</topic><topic>Seeds - immunology</topic><topic>sesame seeds</topic><topic>Sesamum - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolff, Neomi</creatorcontrib><creatorcontrib>Yannai, Shmuel</creatorcontrib><creatorcontrib>Karin, Nathan</creatorcontrib><creatorcontrib>Levy, Yael</creatorcontrib><creatorcontrib>Reifen, Ram</creatorcontrib><creatorcontrib>Dalal, Ilan</creatorcontrib><creatorcontrib>Cogan, Uri</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Allergy and Clinical Immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolff, Neomi</au><au>Yannai, Shmuel</au><au>Karin, Nathan</au><au>Levy, Yael</au><au>Reifen, Ram</au><au>Dalal, Ilan</au><au>Cogan, Uri</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification and characterization of linear B-cell epitopes of β-globulin, a major allergen of sesame seeds</atitle><jtitle>Journal of Allergy and Clinical Immunology</jtitle><addtitle>J Allergy Clin Immunol</addtitle><date>2004-11-01</date><risdate>2004</risdate><volume>114</volume><issue>5</issue><spage>1151</spage><epage>1158</epage><pages>1151-1158</pages><issn>0091-6749</issn><eissn>1097-6825</eissn><eissn>1365-2567</eissn><coden>JACIBY</coden><abstract>The increased consumption of foods containing sesame seeds is paralleled by an increase in reported sesame-induced allergic reactions.
This study aimed at identifying and characterizing the linear B-cell epitopes of the 14-kd β-globulin, the major allergen of sesame seed.
A peptide containing 71 amino acids (peptide B) was previously identified by us as the IgE-binding region on β-globulin. To determine the amino acid sequence of the IgE-binding sites on peptide B, we synthesized overlapping peptides 20 and 10 amino acid residues long that span the entire length of peptide B, which were offset from each other by 10 and 2 amino acid residues, respectively. Sera from 20 subjects given diagnoses of allergy to sesame β-globulin served to identify the epitopes by using the dot-blot test.
At least 9 different IgE-recognition sites were identified on peptide B. Three of them, numbers 2, 3, and 13 (corresponding to amino acids 46-55, 48-57, and 76-86, respectively, in the β-globulin sequence), appeared to be immunodominant IgE-binding epitopes. Also, these peptides were best recognized in terms of intensity of response. There was no obvious sequence motif shared by the 9 different IgE-binding epitopes of β-globulin. However, approximately 60% of the amino acids represented in the epitopes are hydrophobic residues.
Identification of the IgE-binding epitopes might provide a better understanding of the functional role the allergens play in the disease and might have implications for immunodiagnosis and probably immunotherapy.</abstract><cop>New York, NY</cop><pub>Mosby, Inc</pub><pmid>15536424</pmid><doi>10.1016/j.jaci.2004.07.038</doi><tpages>8</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0091-6749 |
ispartof | Journal of Allergy and Clinical Immunology, 2004-11, Vol.114 (5), p.1151-1158 |
issn | 0091-6749 1097-6825 1365-2567 |
language | eng |
recordid | cdi_proquest_miscellaneous_67068568 |
source | MEDLINE; Access via Wiley Online Library; IngentaConnect Free/Open Access Journals; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals; Wiley Online Library (Open Access Collection); PubMed Central |
subjects | Adolescent Adult Allergenicity Allergens - immunology Amino Acid Sequence Beta-Globulins - immunology Biological and medical sciences Child Child, Preschool epitopes Epitopes, B-Lymphocyte Fundamental and applied biological sciences. Psychology Fundamental immunology Humans IgE Immunoglobulin E - immunology Immunopathology Infant major allergen Medical sciences Molecular Sequence Data peptide synthesis Seeds - immunology sesame seeds Sesamum - immunology |
title | Identification and characterization of linear B-cell epitopes of β-globulin, a major allergen of sesame seeds |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T11%3A26%3A07IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20and%20characterization%20of%20linear%20B-cell%20epitopes%20of%20%CE%B2-globulin,%20a%20major%20allergen%20of%20sesame%20seeds&rft.jtitle=Journal%20of%20Allergy%20and%20Clinical%20Immunology&rft.au=Wolff,%20Neomi&rft.date=2004-11-01&rft.volume=114&rft.issue=5&rft.spage=1151&rft.epage=1158&rft.pages=1151-1158&rft.issn=0091-6749&rft.eissn=1097-6825&rft.coden=JACIBY&rft_id=info:doi/10.1016/j.jaci.2004.07.038&rft_dat=%3Cproquest_cross%3E67068568%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=19684002&rft_id=info:pmid/15536424&rft_els_id=S0091674904020834&rfr_iscdi=true |