Optimized large-scale production of high titer lentivirus vector pseudotypes

The goal of the present study was to develop an efficient transient transfection method for large-scale production of high titer lentivirus vector stocks of eight different pseudotypes. The envelope genes used for this purpose were those from VSV-G, Mokola, Rabies, MLV-Ampho, MLV-10A1, LCMV-WE, and...

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Veröffentlicht in:	Journal of virological methods 2004-12, Vol.122 (2), p.131-139
Hauptverfasser:	Sena-Esteves, Miguel, Tebbets, Jessica C., Steffens, Sabine, Crombleholme, Timothy, Flake, Alan W.
Format:	Artikel
Sprache:	eng
Schlagworte:	10A1 Amphotropic Biological and medical sciences Cell Line Ebolavirus - genetics Fundamental and applied biological sciences. Psychology Genetic Vectors - genetics Genetic Vectors - physiology HIV-1 - genetics HIV-1 - physiology LCMV Lentivirus Lentivirus - genetics Lentivirus - physiology Lymphocytic choriomeningitis virus - genetics Microbiology Mokola Plasmids Pseudotype Rabies Techniques used in virology Transduction, Genetic - methods Transfection Vector Vesicular stomatitis Indiana virus - genetics Viral Envelope Proteins - genetics Viral Fusion Proteins - genetics Virology VSV-G
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