DNA polymerase β mRNA and protein expression in Xiphophorus fish
Herein we report Xiphophorus DNA polymerase β (XiphPolβ) mRNA and protein expression levels in brain, liver, gill, and testes tissues from Xiphophorus maculatus, Xiphophorus helleri, and Xiphophorus couchianus parental line fish and two different tumor-bearing Xiphophorus interspecies hybrids. Polym...
Gespeichert in:
| Veröffentlicht in: | Comparative biochemistry and physiology. Toxicology & pharmacology 2004-07, Vol.138 (3), p.325-334 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 334 |
|---|---|
| container_issue | 3 |
| container_start_page | 325 |
| container_title | Comparative biochemistry and physiology. Toxicology & pharmacology |
| container_volume | 138 |
| creator | Heater, Sheila J. Oehlers, Leon P. Rains, J. Douglas Walter, Ronald B. |
| description | Herein we report Xiphophorus DNA polymerase β (XiphPolβ) mRNA and protein expression levels in brain, liver, gill, and testes tissues from Xiphophorus maculatus, Xiphophorus helleri, and Xiphophorus couchianus parental line fish and two different tumor-bearing Xiphophorus interspecies hybrids. Polymerase β protein levels in the Xiphophorus tissues were measured by Western blot, and mRNA was measured with a quantitative real time RT-PCR method which employed cRNA construction to produce accurate calibration curves. We found significant differences in both mRNA and protein levels between the tumor-bearing hybrid animals and the three parental species. However, there were no significant differences in either mRNA levels or protein expression observed between the parental species. Thus, interspecies hybridization results in dysregulation of Polβ expression and this may manifest a modulation in DNA repair capability and susceptibility to latent tumorigenesis. |
| doi_str_mv | 10.1016/j.cca.2004.09.002 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67060097</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1532045604001711</els_id><sourcerecordid>67060097</sourcerecordid><originalsourceid>FETCH-LOGICAL-c264t-15eb245acffdea8deefcfd13c4887aec2e3e2098405396694385b3652c3fafb63</originalsourceid><addsrcrecordid>eNp9kNtKAzEQhoMotlYfwBvZK-92nWw22V28KvUIRUEUvAtpdkJT9mSyFftaPojPZEoL3gkDc-Cfn5mPkHMKCQUqrlaJ1ipJAbIEygQgPSBjWuRFTAUvD0PNWRpDxsWInHi_AgCeUXFMRpRzxvISxmR68zSN-q7eNOiUx-jnO2pewki1VdS7bkDbRvjVO_Tedm0UunfbL7sQbu0jY_3ylBwZVXs82-cJebu7fZ09xPPn-8fZdB7rVGRDTDku0owrbUyFqqgQjTYVZTorilyhTpFhCmWRAWelEGXGCr5ggqeaGWUWgk3I5c43nPWxRj_IxnqNda1a7NZeihwEQJkHId0Jteu8d2hk72yj3EZSkFtuciUDN7nlJqGUgVvYudibrxcNVn8be1BBcL0TYHjx06KTXltsNVbWoR5k1dl_7H8B1rF-KQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67060097</pqid></control><display><type>article</type><title>DNA polymerase β mRNA and protein expression in Xiphophorus fish</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><creator>Heater, Sheila J. ; Oehlers, Leon P. ; Rains, J. Douglas ; Walter, Ronald B.</creator><creatorcontrib>Heater, Sheila J. ; Oehlers, Leon P. ; Rains, J. Douglas ; Walter, Ronald B.</creatorcontrib><description>Herein we report Xiphophorus DNA polymerase β (XiphPolβ) mRNA and protein expression levels in brain, liver, gill, and testes tissues from Xiphophorus maculatus, Xiphophorus helleri, and Xiphophorus couchianus parental line fish and two different tumor-bearing Xiphophorus interspecies hybrids. Polymerase β protein levels in the Xiphophorus tissues were measured by Western blot, and mRNA was measured with a quantitative real time RT-PCR method which employed cRNA construction to produce accurate calibration curves. We found significant differences in both mRNA and protein levels between the tumor-bearing hybrid animals and the three parental species. However, there were no significant differences in either mRNA levels or protein expression observed between the parental species. Thus, interspecies hybridization results in dysregulation of Polβ expression and this may manifest a modulation in DNA repair capability and susceptibility to latent tumorigenesis.</description><identifier>ISSN: 1532-0456</identifier><identifier>EISSN: 1878-1659</identifier><identifier>DOI: 10.1016/j.cca.2004.09.002</identifier><identifier>PMID: 15533790</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Base excision repair ; Base Sequence ; Cyprinodontiformes - genetics ; Cyprinodontiformes - metabolism ; DNA Polymerase beta - genetics ; DNA Polymerase beta - metabolism ; DNA polymerase β ; DNA, Complementary - genetics ; Fish ; Gene expression ; Gene Expression Profiling ; Interspecies hybrids ; Melanoma ; Molecular Sequence Data ; mRNA expression ; Real time RT-PCR ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Western blot</subject><ispartof>Comparative biochemistry and physiology. Toxicology & pharmacology, 2004-07, Vol.138 (3), p.325-334</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c264t-15eb245acffdea8deefcfd13c4887aec2e3e2098405396694385b3652c3fafb63</citedby><cites>FETCH-LOGICAL-c264t-15eb245acffdea8deefcfd13c4887aec2e3e2098405396694385b3652c3fafb63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.cca.2004.09.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15533790$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Heater, Sheila J.</creatorcontrib><creatorcontrib>Oehlers, Leon P.</creatorcontrib><creatorcontrib>Rains, J. Douglas</creatorcontrib><creatorcontrib>Walter, Ronald B.</creatorcontrib><title>DNA polymerase β mRNA and protein expression in Xiphophorus fish</title><title>Comparative biochemistry and physiology. Toxicology & pharmacology</title><addtitle>Comp Biochem Physiol C Toxicol Pharmacol</addtitle><description>Herein we report Xiphophorus DNA polymerase β (XiphPolβ) mRNA and protein expression levels in brain, liver, gill, and testes tissues from Xiphophorus maculatus, Xiphophorus helleri, and Xiphophorus couchianus parental line fish and two different tumor-bearing Xiphophorus interspecies hybrids. Polymerase β protein levels in the Xiphophorus tissues were measured by Western blot, and mRNA was measured with a quantitative real time RT-PCR method which employed cRNA construction to produce accurate calibration curves. We found significant differences in both mRNA and protein levels between the tumor-bearing hybrid animals and the three parental species. However, there were no significant differences in either mRNA levels or protein expression observed between the parental species. Thus, interspecies hybridization results in dysregulation of Polβ expression and this may manifest a modulation in DNA repair capability and susceptibility to latent tumorigenesis.</description><subject>Animals</subject><subject>Base excision repair</subject><subject>Base Sequence</subject><subject>Cyprinodontiformes - genetics</subject><subject>Cyprinodontiformes - metabolism</subject><subject>DNA Polymerase beta - genetics</subject><subject>DNA Polymerase beta - metabolism</subject><subject>DNA polymerase β</subject><subject>DNA, Complementary - genetics</subject><subject>Fish</subject><subject>Gene expression</subject><subject>Gene Expression Profiling</subject><subject>Interspecies hybrids</subject><subject>Melanoma</subject><subject>Molecular Sequence Data</subject><subject>mRNA expression</subject><subject>Real time RT-PCR</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Western blot</subject><issn>1532-0456</issn><issn>1878-1659</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2004</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kNtKAzEQhoMotlYfwBvZK-92nWw22V28KvUIRUEUvAtpdkJT9mSyFftaPojPZEoL3gkDc-Cfn5mPkHMKCQUqrlaJ1ipJAbIEygQgPSBjWuRFTAUvD0PNWRpDxsWInHi_AgCeUXFMRpRzxvISxmR68zSN-q7eNOiUx-jnO2pewki1VdS7bkDbRvjVO_Tedm0UunfbL7sQbu0jY_3ylBwZVXs82-cJebu7fZ09xPPn-8fZdB7rVGRDTDku0owrbUyFqqgQjTYVZTorilyhTpFhCmWRAWelEGXGCr5ggqeaGWUWgk3I5c43nPWxRj_IxnqNda1a7NZeihwEQJkHId0Jteu8d2hk72yj3EZSkFtuciUDN7nlJqGUgVvYudibrxcNVn8be1BBcL0TYHjx06KTXltsNVbWoR5k1dl_7H8B1rF-KQ</recordid><startdate>200407</startdate><enddate>200407</enddate><creator>Heater, Sheila J.</creator><creator>Oehlers, Leon P.</creator><creator>Rains, J. Douglas</creator><creator>Walter, Ronald B.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200407</creationdate><title>DNA polymerase β mRNA and protein expression in Xiphophorus fish</title><author>Heater, Sheila J. ; Oehlers, Leon P. ; Rains, J. Douglas ; Walter, Ronald B.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c264t-15eb245acffdea8deefcfd13c4887aec2e3e2098405396694385b3652c3fafb63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2004</creationdate><topic>Animals</topic><topic>Base excision repair</topic><topic>Base Sequence</topic><topic>Cyprinodontiformes - genetics</topic><topic>Cyprinodontiformes - metabolism</topic><topic>DNA Polymerase beta - genetics</topic><topic>DNA Polymerase beta - metabolism</topic><topic>DNA polymerase β</topic><topic>DNA, Complementary - genetics</topic><topic>Fish</topic><topic>Gene expression</topic><topic>Gene Expression Profiling</topic><topic>Interspecies hybrids</topic><topic>Melanoma</topic><topic>Molecular Sequence Data</topic><topic>mRNA expression</topic><topic>Real time RT-PCR</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Western blot</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Heater, Sheila J.</creatorcontrib><creatorcontrib>Oehlers, Leon P.</creatorcontrib><creatorcontrib>Rains, J. Douglas</creatorcontrib><creatorcontrib>Walter, Ronald B.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Comparative biochemistry and physiology. Toxicology & pharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Heater, Sheila J.</au><au>Oehlers, Leon P.</au><au>Rains, J. Douglas</au><au>Walter, Ronald B.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>DNA polymerase β mRNA and protein expression in Xiphophorus fish</atitle><jtitle>Comparative biochemistry and physiology. Toxicology & pharmacology</jtitle><addtitle>Comp Biochem Physiol C Toxicol Pharmacol</addtitle><date>2004-07</date><risdate>2004</risdate><volume>138</volume><issue>3</issue><spage>325</spage><epage>334</epage><pages>325-334</pages><issn>1532-0456</issn><eissn>1878-1659</eissn><abstract>Herein we report Xiphophorus DNA polymerase β (XiphPolβ) mRNA and protein expression levels in brain, liver, gill, and testes tissues from Xiphophorus maculatus, Xiphophorus helleri, and Xiphophorus couchianus parental line fish and two different tumor-bearing Xiphophorus interspecies hybrids. Polymerase β protein levels in the Xiphophorus tissues were measured by Western blot, and mRNA was measured with a quantitative real time RT-PCR method which employed cRNA construction to produce accurate calibration curves. We found significant differences in both mRNA and protein levels between the tumor-bearing hybrid animals and the three parental species. However, there were no significant differences in either mRNA levels or protein expression observed between the parental species. Thus, interspecies hybridization results in dysregulation of Polβ expression and this may manifest a modulation in DNA repair capability and susceptibility to latent tumorigenesis.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15533790</pmid><doi>10.1016/j.cca.2004.09.002</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1532-0456 |
| ispartof | Comparative biochemistry and physiology. Toxicology & pharmacology, 2004-07, Vol.138 (3), p.325-334 |
| issn | 1532-0456 1878-1659 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67060097 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Base excision repair Base Sequence Cyprinodontiformes - genetics Cyprinodontiformes - metabolism DNA Polymerase beta - genetics DNA Polymerase beta - metabolism DNA polymerase β DNA, Complementary - genetics Fish Gene expression Gene Expression Profiling Interspecies hybrids Melanoma Molecular Sequence Data mRNA expression Real time RT-PCR RNA, Messenger - genetics RNA, Messenger - metabolism Western blot |
| title | DNA polymerase β mRNA and protein expression in Xiphophorus fish |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-03T03%3A33%3A13IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=DNA%20polymerase%20%CE%B2%20mRNA%20and%20protein%20expression%20in%20Xiphophorus%20fish&rft.jtitle=Comparative%20biochemistry%20and%20physiology.%20Toxicology%20&%20pharmacology&rft.au=Heater,%20Sheila%20J.&rft.date=2004-07&rft.volume=138&rft.issue=3&rft.spage=325&rft.epage=334&rft.pages=325-334&rft.issn=1532-0456&rft.eissn=1878-1659&rft_id=info:doi/10.1016/j.cca.2004.09.002&rft_dat=%3Cproquest_cross%3E67060097%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67060097&rft_id=info:pmid/15533790&rft_els_id=S1532045604001711&rfr_iscdi=true |