Biosynthesis of salicylic acid in fungus elicited Catharanthus roseus cells
Feeding experiments using [1- 13C]- d-glucose to Catharanthus roseus (L.) G.Don cell suspension cultures were performed in order to study the salicylic acid (SA) biosynthetic pathway and that of 2,3-dihydroxybenzoic acid (2,3-DHBA) as a comparison. Relatively high- and non-symmetrical enrichment rat...
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| Veröffentlicht in: | Phytochemistry (Oxford) 2009-03, Vol.70 (4), p.532-539 |
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| creator | Mustafa, Natali R. Kim, Hye Kyong Choi, Young Hae Erkelens, Cornelis Lefeber, Alfons W.M. Spijksma, Gerwin Heijden, Robert van der Verpoorte, Robert |
| description | Feeding experiments using [1-
13C]-
d-glucose to
Catharanthus roseus (L.) G.Don cell suspension cultures were performed in order to study the salicylic acid (SA) biosynthetic pathway and that of 2,3-dihydroxybenzoic acid (2,3-DHBA) as a comparison. Relatively high- and non-symmetrical enrichment ratios at C-2 and C-6, and a lower enrichment ratio at C-7 were observed in both SA and 2,3-DHBA detected by
13C NMR inverse gated spectrometry leading to the conclusion that the isochorismate pathway is responsible for the biosynthesis of both compounds rather than the phenylpropanoid pathway.
Feeding experiments using [1-
13C]-
d-glucose to
Catharanthus roseus (L.) G.Don cell suspension cultures followed by elicitation with
Pythium aphanidermatum extract were performed in order to study the salicylic acid (SA) biosynthetic pathway and that of 2,3-dihydroxybenzoic acid (2,3-DHBA) as a comparison. A strongly labeled C-7 and a symmetrical partitioning of the label between C-2 and C-6 would occur if SA was synthesized from phenylalanine. In case of the isochorismate pathway, a relatively lower incorporation at C-7 and a non-symmetrical incorporation at C-2 and C-6 would be obtained. Relatively, high- and non-symmetrical enrichment ratios at C-2 and C-6, and a lower enrichment ratio at C-7 were observed in both SA and 2,3-DHBA detected by
13C NMR inverse gated spectrometry leading to the conclusion that the isochorismate pathway is responsible for the biosynthesis of both compounds. However, different enrichment ratios of the labeled carbons in SA and 2,3-DHBA indicate the use of different isochorismate pools, which means that their biosynthesis is separated in time and/or space. |
| doi_str_mv | 10.1016/j.phytochem.2009.01.009 |
| format | Article |
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13C]-
d-glucose to
Catharanthus roseus (L.) G.Don cell suspension cultures were performed in order to study the salicylic acid (SA) biosynthetic pathway and that of 2,3-dihydroxybenzoic acid (2,3-DHBA) as a comparison. Relatively high- and non-symmetrical enrichment ratios at C-2 and C-6, and a lower enrichment ratio at C-7 were observed in both SA and 2,3-DHBA detected by
13C NMR inverse gated spectrometry leading to the conclusion that the isochorismate pathway is responsible for the biosynthesis of both compounds rather than the phenylpropanoid pathway.
Feeding experiments using [1-
13C]-
d-glucose to
Catharanthus roseus (L.) G.Don cell suspension cultures followed by elicitation with
Pythium aphanidermatum extract were performed in order to study the salicylic acid (SA) biosynthetic pathway and that of 2,3-dihydroxybenzoic acid (2,3-DHBA) as a comparison. A strongly labeled C-7 and a symmetrical partitioning of the label between C-2 and C-6 would occur if SA was synthesized from phenylalanine. In case of the isochorismate pathway, a relatively lower incorporation at C-7 and a non-symmetrical incorporation at C-2 and C-6 would be obtained. Relatively, high- and non-symmetrical enrichment ratios at C-2 and C-6, and a lower enrichment ratio at C-7 were observed in both SA and 2,3-DHBA detected by
13C NMR inverse gated spectrometry leading to the conclusion that the isochorismate pathway is responsible for the biosynthesis of both compounds. However, different enrichment ratios of the labeled carbons in SA and 2,3-DHBA indicate the use of different isochorismate pools, which means that their biosynthesis is separated in time and/or space.</description><identifier>ISSN: 0031-9422</identifier><identifier>EISSN: 1873-3700</identifier><identifier>DOI: 10.1016/j.phytochem.2009.01.009</identifier><identifier>PMID: 19251288</identifier><language>eng</language><publisher>Amsterdam: Elsevier Ltd</publisher><subject>2,3-Dihydroxybenzoic acid ; benozoic acid 2-hydroxylase ; biochemical pathways ; Biological and medical sciences ; Carbon Isotopes ; Catharanthus - metabolism ; Catharanthus - microbiology ; Catharanthus roseus ; Catharanthus roseus cell suspension cultures ; cell suspension culture ; Cells, Cultured ; Chemical constitution ; cultured cells ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Hydroxybenzoates - chemistry ; Hydroxybenzoates - metabolism ; Isochorismate pathway ; isochorismate pyruvate-lyase ; isochorismate synthase ; Medical sciences ; Nuclear Magnetic Resonance, Biomolecular ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; phenylalanine ammonia-lyase ; Phenylalanine pathway ; Plant physiology and development ; Pythium - physiology ; Pythium aphanidermatum ; salicylic acid ; Salicylic Acid - chemistry ; Salicylic Acid - metabolism ; Salicylic acid biosynthesis</subject><ispartof>Phytochemistry (Oxford), 2009-03, Vol.70 (4), p.532-539</ispartof><rights>2009 Elsevier Ltd</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c520t-12011a6c83b549f63aff751a68565b074e2753556e59b7479d94371c4791d92e3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0031942209000351$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21415557$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19251288$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mustafa, Natali R.</creatorcontrib><creatorcontrib>Kim, Hye Kyong</creatorcontrib><creatorcontrib>Choi, Young Hae</creatorcontrib><creatorcontrib>Erkelens, Cornelis</creatorcontrib><creatorcontrib>Lefeber, Alfons W.M.</creatorcontrib><creatorcontrib>Spijksma, Gerwin</creatorcontrib><creatorcontrib>Heijden, Robert van der</creatorcontrib><creatorcontrib>Verpoorte, Robert</creatorcontrib><title>Biosynthesis of salicylic acid in fungus elicited Catharanthus roseus cells</title><title>Phytochemistry (Oxford)</title><addtitle>Phytochemistry</addtitle><description>Feeding experiments using [1-
13C]-
d-glucose to
Catharanthus roseus (L.) G.Don cell suspension cultures were performed in order to study the salicylic acid (SA) biosynthetic pathway and that of 2,3-dihydroxybenzoic acid (2,3-DHBA) as a comparison. Relatively high- and non-symmetrical enrichment ratios at C-2 and C-6, and a lower enrichment ratio at C-7 were observed in both SA and 2,3-DHBA detected by
13C NMR inverse gated spectrometry leading to the conclusion that the isochorismate pathway is responsible for the biosynthesis of both compounds rather than the phenylpropanoid pathway.
Feeding experiments using [1-
13C]-
d-glucose to
Catharanthus roseus (L.) G.Don cell suspension cultures followed by elicitation with
Pythium aphanidermatum extract were performed in order to study the salicylic acid (SA) biosynthetic pathway and that of 2,3-dihydroxybenzoic acid (2,3-DHBA) as a comparison. A strongly labeled C-7 and a symmetrical partitioning of the label between C-2 and C-6 would occur if SA was synthesized from phenylalanine. In case of the isochorismate pathway, a relatively lower incorporation at C-7 and a non-symmetrical incorporation at C-2 and C-6 would be obtained. Relatively, high- and non-symmetrical enrichment ratios at C-2 and C-6, and a lower enrichment ratio at C-7 were observed in both SA and 2,3-DHBA detected by
13C NMR inverse gated spectrometry leading to the conclusion that the isochorismate pathway is responsible for the biosynthesis of both compounds. However, different enrichment ratios of the labeled carbons in SA and 2,3-DHBA indicate the use of different isochorismate pools, which means that their biosynthesis is separated in time and/or space.</description><subject>2,3-Dihydroxybenzoic acid</subject><subject>benozoic acid 2-hydroxylase</subject><subject>biochemical pathways</subject><subject>Biological and medical sciences</subject><subject>Carbon Isotopes</subject><subject>Catharanthus - metabolism</subject><subject>Catharanthus - microbiology</subject><subject>Catharanthus roseus</subject><subject>Catharanthus roseus cell suspension cultures</subject><subject>cell suspension culture</subject><subject>Cells, Cultured</subject><subject>Chemical constitution</subject><subject>cultured cells</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Hydroxybenzoates - chemistry</subject><subject>Hydroxybenzoates - metabolism</subject><subject>Isochorismate pathway</subject><subject>isochorismate pyruvate-lyase</subject><subject>isochorismate synthase</subject><subject>Medical sciences</subject><subject>Nuclear Magnetic Resonance, Biomolecular</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>phenylalanine ammonia-lyase</subject><subject>Phenylalanine pathway</subject><subject>Plant physiology and development</subject><subject>Pythium - physiology</subject><subject>Pythium aphanidermatum</subject><subject>salicylic acid</subject><subject>Salicylic Acid - chemistry</subject><subject>Salicylic Acid - metabolism</subject><subject>Salicylic acid biosynthesis</subject><issn>0031-9422</issn><issn>1873-3700</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE-P0zAQxS0EYsvCV2BzgVvCjB3b8XGp-CdW4gB7tlxnsnWVJiVOVuq3Z6pWy3EP1rOefuN5fkLcIFQIaD7tqsP2OI9xS_tKArgKsGJ5IVbYWFUqC_BSrAAUlq6W8kq8yXkHAFob81pcoZMaZdOsxM_PaczHYd5STrkYuyKHPsUjnyLE1BZpKLpleFhyQeylmdpiHeZtmALPsDuNmVgi9X1-K151oc_07qLX4v7rlz_r7-Xdr28_1rd3ZdQS5hIlIAYTG7XRteuMCl1nNTuNNnoDtiZpteKkpN3G1ta1rlYWI9-wdZLUtfh4fvcwjX8XyrPfp3xKEAYal-yNBQNg3LOgBN00qCyD9gxG_k-eqPOHKe3DdPQI_lS43_mnwv2pcA_oWXjy_WXFstlT-3_u0jADHy5AyDH0HRcXU37iJNaotT5FuDlzXRh9eJiYuf_NTSnejlI5ycTtmSDu9jHR5HNMNERq00Rx9u2Yno37D_X4qvs</recordid><startdate>20090301</startdate><enddate>20090301</enddate><creator>Mustafa, Natali R.</creator><creator>Kim, Hye Kyong</creator><creator>Choi, Young Hae</creator><creator>Erkelens, Cornelis</creator><creator>Lefeber, Alfons W.M.</creator><creator>Spijksma, Gerwin</creator><creator>Heijden, Robert van der</creator><creator>Verpoorte, Robert</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>M7N</scope><scope>7X8</scope></search><sort><creationdate>20090301</creationdate><title>Biosynthesis of salicylic acid in fungus elicited Catharanthus roseus cells</title><author>Mustafa, Natali R. ; Kim, Hye Kyong ; Choi, Young Hae ; Erkelens, Cornelis ; Lefeber, Alfons W.M. ; Spijksma, Gerwin ; Heijden, Robert van der ; Verpoorte, Robert</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c520t-12011a6c83b549f63aff751a68565b074e2753556e59b7479d94371c4791d92e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>2,3-Dihydroxybenzoic acid</topic><topic>benozoic acid 2-hydroxylase</topic><topic>biochemical pathways</topic><topic>Biological and medical sciences</topic><topic>Carbon Isotopes</topic><topic>Catharanthus - metabolism</topic><topic>Catharanthus - microbiology</topic><topic>Catharanthus roseus</topic><topic>Catharanthus roseus cell suspension cultures</topic><topic>cell suspension culture</topic><topic>Cells, Cultured</topic><topic>Chemical constitution</topic><topic>cultured cells</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Hydroxybenzoates - chemistry</topic><topic>Hydroxybenzoates - metabolism</topic><topic>Isochorismate pathway</topic><topic>isochorismate pyruvate-lyase</topic><topic>isochorismate synthase</topic><topic>Medical sciences</topic><topic>Nuclear Magnetic Resonance, Biomolecular</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>phenylalanine ammonia-lyase</topic><topic>Phenylalanine pathway</topic><topic>Plant physiology and development</topic><topic>Pythium - physiology</topic><topic>Pythium aphanidermatum</topic><topic>salicylic acid</topic><topic>Salicylic Acid - chemistry</topic><topic>Salicylic Acid - metabolism</topic><topic>Salicylic acid biosynthesis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mustafa, Natali R.</creatorcontrib><creatorcontrib>Kim, Hye Kyong</creatorcontrib><creatorcontrib>Choi, Young Hae</creatorcontrib><creatorcontrib>Erkelens, Cornelis</creatorcontrib><creatorcontrib>Lefeber, Alfons W.M.</creatorcontrib><creatorcontrib>Spijksma, Gerwin</creatorcontrib><creatorcontrib>Heijden, Robert van der</creatorcontrib><creatorcontrib>Verpoorte, Robert</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><jtitle>Phytochemistry (Oxford)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mustafa, Natali R.</au><au>Kim, Hye Kyong</au><au>Choi, Young Hae</au><au>Erkelens, Cornelis</au><au>Lefeber, Alfons W.M.</au><au>Spijksma, Gerwin</au><au>Heijden, Robert van der</au><au>Verpoorte, Robert</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Biosynthesis of salicylic acid in fungus elicited Catharanthus roseus cells</atitle><jtitle>Phytochemistry (Oxford)</jtitle><addtitle>Phytochemistry</addtitle><date>2009-03-01</date><risdate>2009</risdate><volume>70</volume><issue>4</issue><spage>532</spage><epage>539</epage><pages>532-539</pages><issn>0031-9422</issn><eissn>1873-3700</eissn><abstract>Feeding experiments using [1-
13C]-
d-glucose to
Catharanthus roseus (L.) G.Don cell suspension cultures were performed in order to study the salicylic acid (SA) biosynthetic pathway and that of 2,3-dihydroxybenzoic acid (2,3-DHBA) as a comparison. Relatively high- and non-symmetrical enrichment ratios at C-2 and C-6, and a lower enrichment ratio at C-7 were observed in both SA and 2,3-DHBA detected by
13C NMR inverse gated spectrometry leading to the conclusion that the isochorismate pathway is responsible for the biosynthesis of both compounds rather than the phenylpropanoid pathway.
Feeding experiments using [1-
13C]-
d-glucose to
Catharanthus roseus (L.) G.Don cell suspension cultures followed by elicitation with
Pythium aphanidermatum extract were performed in order to study the salicylic acid (SA) biosynthetic pathway and that of 2,3-dihydroxybenzoic acid (2,3-DHBA) as a comparison. A strongly labeled C-7 and a symmetrical partitioning of the label between C-2 and C-6 would occur if SA was synthesized from phenylalanine. In case of the isochorismate pathway, a relatively lower incorporation at C-7 and a non-symmetrical incorporation at C-2 and C-6 would be obtained. Relatively, high- and non-symmetrical enrichment ratios at C-2 and C-6, and a lower enrichment ratio at C-7 were observed in both SA and 2,3-DHBA detected by
13C NMR inverse gated spectrometry leading to the conclusion that the isochorismate pathway is responsible for the biosynthesis of both compounds. However, different enrichment ratios of the labeled carbons in SA and 2,3-DHBA indicate the use of different isochorismate pools, which means that their biosynthesis is separated in time and/or space.</abstract><cop>Amsterdam</cop><pub>Elsevier Ltd</pub><pmid>19251288</pmid><doi>10.1016/j.phytochem.2009.01.009</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Phytochemistry (Oxford), 2009-03, Vol.70 (4), p.532-539 |
| issn | 0031-9422 1873-3700 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 2,3-Dihydroxybenzoic acid benozoic acid 2-hydroxylase biochemical pathways Biological and medical sciences Carbon Isotopes Catharanthus - metabolism Catharanthus - microbiology Catharanthus roseus Catharanthus roseus cell suspension cultures cell suspension culture Cells, Cultured Chemical constitution cultured cells Fundamental and applied biological sciences. Psychology General pharmacology Hydroxybenzoates - chemistry Hydroxybenzoates - metabolism Isochorismate pathway isochorismate pyruvate-lyase isochorismate synthase Medical sciences Nuclear Magnetic Resonance, Biomolecular Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments phenylalanine ammonia-lyase Phenylalanine pathway Plant physiology and development Pythium - physiology Pythium aphanidermatum salicylic acid Salicylic Acid - chemistry Salicylic Acid - metabolism Salicylic acid biosynthesis |
| title | Biosynthesis of salicylic acid in fungus elicited Catharanthus roseus cells |
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